EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Visualizing the Assembly Pathway of Nucleolar Pre-60S Ribosomes.
ジャーナル・号・ページ	Cell, Vol. 171, Issue 7, Page 1599-1610.e14, Year 2017
掲載日	2017年12月14日
著者	Lukas Kater / Matthias Thoms / Clara Barrio-Garcia / Jingdong Cheng / Sherif Ismail / Yasar Luqman Ahmed / Gert Bange / Dieter Kressler / Otto Berninghausen / Irmgard Sinning / Ed Hurt / Roland Beckmann /
PubMed 要旨	Eukaryotic 60S ribosomal subunits are comprised of three rRNAs and ∼50 ribosomal proteins. The initial steps of their formation take place in the nucleolus, but, owing to a lack of structural ...Eukaryotic 60S ribosomal subunits are comprised of three rRNAs and ∼50 ribosomal proteins. The initial steps of their formation take place in the nucleolus, but, owing to a lack of structural information, this process is poorly understood. Using cryo-EM, we solved structures of early 60S biogenesis intermediates at 3.3 Å to 4.5 Å resolution, thereby providing insights into their sequential folding and assembly pathway. Besides revealing distinct immature rRNA conformations, we map 25 assembly factors in six different assembly states. Notably, the Nsa1-Rrp1-Rpf1-Mak16 module stabilizes the solvent side of the 60S subunit, and the Erb1-Ytm1-Nop7 complex organizes and connects through Erb1's meandering N-terminal extension, eight assembly factors, three ribosomal proteins, and three 25S rRNA domains. Our structural snapshots reveal the order of integration and compaction of the six major 60S domains within early nucleolar 60S particles developing stepwise from the solvent side around the exit tunnel to the central protuberance.
リンク	Cell / PubMed:29245012 / PubMed Central
手法	EM (単粒子) / X線回折
解像度	2.24 - 4.3 Å
構造データ	3888 6em3 EMDB-3888: State A (Nsa1-TAP Flag-Ytm1) - Visualizing the assembly pathway of nucleolar pre-60S ribosomes PDB-6em3: State A architectural model (Nsa1-TAP Flag-Ytm1) - Visualizing the assembly pathway of nucleolar pre-60S ribosomes 手法: EM (単粒子) / 解像度: 4.2 Å 3889 6em4 EMDB-3889: State B (Nsa1-TAP Flag-Ytm1) - Visualizing the assembly pathway of nucleolar pre-60S ribosomes PDB-6em4: State B architectural model (Nsa1-TAP Flag-Ytm1) - Visualizing the assembly pathway of nucleolar pre-60S ribosomes 手法: EM (単粒子) / 解像度: 4.1 Å 3890 6em5 EMDB-3890: State D (Nsa1-TAP Flag-Ytm1) - Visualizing the assembly pathway of nucleolar pre-60S ribosomes PDB-6em5: State D architectural model (Nsa1-TAP Flag-Ytm1) - Visualizing the assembly pathway of nucleolar pre-60S ribosomes 手法: EM (単粒子) / 解像度: 4.2 Å 3891 6elz EMDB-3891, PDB-6elz: State E (TAP-Flag-Ytm1 E80A) - Visualizing the assembly pathway of nucleolar pre-60S ribosomes 手法: EM (単粒子) / 解像度: 3.3 Å EMDB-3892: State F (Rix1-TAP Rpf2-Flag) - Visualizing the assembly pathway of nucleolar pre-60S ribosomes 手法: EM (単粒子) / 解像度: 3.3 Å 3893 6em1 EMDB-3893, PDB-6em1: State C (Nsa1-TAP Flag-Ytm1) - Visualizing the assembly pathway of nucleolar pre-60S ribosomes 手法: EM (単粒子) / 解像度: 3.6 Å PDB-6emf: Crystal structure of Rrp1 from Chaetomium thermophilum in space group C2 手法: X-RAY DIFFRACTION / 解像度: 2.65 Å PDB-6emg: Crystal structure of Rrp1 from Chaetomium thermophilum in space group P6322 手法: X-RAY DIFFRACTION / 解像度: 2.24 Å PDB-6en7: Crystal structure of the ribosome assembly factor Nsa1 手法: X-RAY DIFFRACTION / 解像度: 2.403 Å
化合物	ChemComp-ZN: Unknown entry ChemComp-EDO: 1,2-ETHANEDIOL / エチレングリコ-ル / エチレングリコール ChemComp-PRO: PROLINE / プロリン / プロリン ChemComp-HOH: WATER / 水 ChemComp-PO4: PHOSPHATE ION / ホスファ-ト / リン酸塩 ChemComp-EPE: 4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID / HEPES / pH緩衝剤*YM / HEPES
由来	saccharomyces cerevisiae (パン酵母) saccharomyces cerevisiae s288c (パン酵母) saccharomyces cerevisiae (strain atcc 204508 / s288c) (パン酵母) chaetomium thermophilum (strain dsm 1495 / cbs 144.50 / imi 039719) (菌類)
キーワード	RIBOSOME (リボソーム) / Large Subunit Biogenesis Nucleolus / Ribosome biogenesis (リボソーム生合成) / translation (翻訳 (生物学)) / CHAPERONE (シャペロン) / biogenesis (自然発生説)