EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Virion structure and genome delivery mechanism of sacbrood honeybee virus.
ジャーナル・号・ページ	Proc Natl Acad Sci U S A, Vol. 115, Issue 30, Page 7759-7764, Year 2018
掲載日	2018年7月24日
著者	Michaela Procházková / Tibor Füzik / Karel Škubník / Jana Moravcová / Zorica Ubiparip / Antonín Přidal / Pavel Plevka /
PubMed 要旨	Infection by sacbrood virus (SBV) from the family Iflaviridae is lethal to honey bee larvae but only rarely causes the collapse of honey bee colonies. Despite the negative effect of SBV on honey ...Infection by sacbrood virus (SBV) from the family Iflaviridae is lethal to honey bee larvae but only rarely causes the collapse of honey bee colonies. Despite the negative effect of SBV on honey bees, the structure of its particles and mechanism of its genome delivery are unknown. Here we present the crystal structure of SBV virion and show that it contains 60 copies of a minor capsid protein (MiCP) attached to the virion surface. No similar MiCPs have been previously reported in any of the related viruses from the order Picornavirales. The location of the MiCP coding sequence within the SBV genome indicates that the MiCP evolved from a C-terminal extension of a major capsid protein by the introduction of a cleavage site for a virus protease. The exposure of SBV to acidic pH, which the virus likely encounters during cell entry, induces the formation of pores at threefold and fivefold axes of the capsid that are 7 Å and 12 Å in diameter, respectively. This is in contrast to vertebrate picornaviruses, in which the pores along twofold icosahedral symmetry axes are currently considered the most likely sites for genome release. SBV virions lack VP4 subunits that facilitate the genome delivery of many related dicistroviruses and picornaviruses. MiCP subunits induce liposome disruption in vitro, indicating that they are functional analogs of VP4 subunits and enable the virus genome to escape across the endosome membrane into the cell cytoplasm.
リンク	Proc Natl Acad Sci U S A / PubMed:29987012 / PubMed Central
手法	EM (単粒子) / X線回折
解像度	2.1 - 7.25 Å
構造データ	3863 5oyp EMDB-3863, PDB-5oyp: Sacbrood virus of honeybee 手法: EM (単粒子) / 解像度: 3.22 Å 3865 6egv EMDB-3865, PDB-6egv: Sacbrood virus of honeybee 手法: EM (単粒子) / 解像度: 3.18 Å 3866 6egx EMDB-3866, PDB-6egx: Sacbrood virus of honeybee - expansion state I 手法: EM (単粒子) / 解像度: 4.06 Å 3867 6eh1 EMDB-3867, PDB-6eh1: Sacbrood virus of honeybee - expansion state II 手法: EM (単粒子) / 解像度: 7.25 Å 3881 6eiw EMDB-3881, PDB-6eiw: Sacbrood virus of honeybee empty particle 手法: EM (単粒子) / 解像度: 3.87 Å PDB-5lsf: Sacbrood honeybee virus 手法: X-RAY DIFFRACTION / 解像度: 2.1 Å
化合物	ChemComp-CA: Unknown entry / カルシウムジカチオン ChemComp-HOH: WATER
由来	sacbrood virus (ウイルス)
キーワード	VIRUS / Iflaviridae / external VP4 / native particle / icosahedral virus particle / iflavirus / full particle / empty particle / expanded particle