EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Mechanism of 5S RNP recruitment and helicase-surveilled rRNA maturation during pre-60S biogenesis.
ジャーナル・号・ページ	EMBO Rep, Vol. 24, Issue 7, Page e56910, Year 2023
掲載日	2023年7月5日
著者	Benjamin Lau / Zixuan Huang / Nikola Kellner / Shuangshuang Niu / Otto Berninghausen / Roland Beckmann / Ed Hurt / Jingdong Cheng /
PubMed 要旨	Ribosome biogenesis proceeds along a multifaceted pathway from the nucleolus to the cytoplasm that is extensively coupled to several quality control mechanisms. However, the mode by which 5S ...Ribosome biogenesis proceeds along a multifaceted pathway from the nucleolus to the cytoplasm that is extensively coupled to several quality control mechanisms. However, the mode by which 5S ribosomal RNA is incorporated into the developing pre-60S ribosome, which in humans links ribosome biogenesis to cell proliferation by surveillance by factors such as p53-MDM2, is poorly understood. Here, we report nine nucleolar pre-60S cryo-EM structures from Chaetomium thermophilum, one of which clarifies the mechanism of 5S RNP incorporation into the early pre-60S. Successive assembly states then represent how helicases Dbp10 and Spb4, and the Pumilio domain factor Puf6 act in series to surveil the gradual folding of the nearby 25S rRNA domain IV. Finally, the methyltransferase Spb1 methylates a universally conserved guanine nucleotide in the A-loop of the peptidyl transferase center, thereby licensing further maturation. Our findings provide insight into the hierarchical action of helicases in safeguarding rRNA tertiary structure folding and coupling to surveillance mechanisms that culminate in local RNA modification.
リンク	EMBO Rep / PubMed:37129998 / PubMed Central
手法	EM (単粒子)
解像度	2.7 - 3.6 Å
構造データ	35279 8i9p EMDB-35279, PDB-8i9p: Cryo-EM structure of a Chaetomium thermophilum pre-60S ribosomal subunit - State Mak16 手法: EM (単粒子) / 解像度: 3.0 Å 35281 8i9r EMDB-35281, PDB-8i9r: Cryo-EM structure of a Chaetomium thermophilum pre-60S ribosomal subunit - State 5S RNP 手法: EM (単粒子) / 解像度: 3.1 Å 35283 8i9t EMDB-35283, PDB-8i9t: Cryo-EM structure of a Chaetomium thermophilum pre-60S ribosomal subunit - State Dbp10-1 手法: EM (単粒子) / 解像度: 3.6 Å 35285 8i9v EMDB-35285, PDB-8i9v: Cryo-EM structure of a Chaetomium thermophilum pre-60S ribosomal subunit - State Dbp10-2 手法: EM (単粒子) / 解像度: 3.1 Å 35286 8i9w EMDB-35286, PDB-8i9w: Cryo-EM structure of a Chaetomium thermophilum pre-60S ribosomal subunit - Dbp10-3 手法: EM (単粒子) / 解像度: 3.1 Å 35287 8i9x EMDB-35287, PDB-8i9x: Cryo-EM structure of a Chaetomium thermophilum pre-60S ribosomal subunit - Ytm1-1 手法: EM (単粒子) / 解像度: 2.8 Å 35288 8i9y EMDB-35288, PDB-8i9y: Cryo-EM structure of a Chaetomium thermophilum pre-60S ribosomal subunit - Ytm1-2 手法: EM (単粒子) / 解像度: 3.1 Å 35289 8i9z EMDB-35289, PDB-8i9z: Cryo-EM structure of a Chaetomium thermophilum pre-60S ribosomal subunit - State Spb4 手法: EM (単粒子) / 解像度: 2.7 Å 35290 8ia0 EMDB-35290, PDB-8ia0: Cryo-EM structure of a Chaetomium thermophilum pre-60S ribosomal subunit - State Puf6 手法: EM (単粒子) / 解像度: 2.7 Å
化合物	ChemComp-ZN: Unknown entry ChemComp-GTP: GUANOSINE-5'-TRIPHOSPHATE / GTP / GTP, エネルギー貯蔵分子YM ChemComp-ADP: ADENOSINE-5'-DIPHOSPHATE / ADP / ADP, エネルギー貯蔵分子YM ChemComp-MG: Unknown entry / マグネシウムジカチオン
由来	chaetomium thermophilum (菌類)
キーワード	RIBOSOME / Ribosome biogenesis / pre-60S ribosome