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- EMDB-35279: Cryo-EM structure of a Chaetomium thermophilum pre-60S ribosomal ... -

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Basic information

Entry
Database: EMDB / ID: EMD-35279
TitleCryo-EM structure of a Chaetomium thermophilum pre-60S ribosomal subunit - State Mak16
Map datalocal resolution filtered map
Sample
  • Complex: pre-60S ribosome
    • Complex: ribosome
      • RNA: x 2 types
      • Protein or peptide: x 30 types
  • Ligand: x 1 types
KeywordsRibosome / Ribosome biogenesis / pre-60S ribosome
Function / homology
Function and homology information


dolichyl-diphosphooligosaccharide-protein glycotransferase / dolichyl-diphosphooligosaccharide-protein glycotransferase activity / rRNA primary transcript binding / maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / preribosome, small subunit precursor / protein glycosylation / preribosome, large subunit precursor / ribonucleoprotein complex binding / maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / cytosolic ribosome ...dolichyl-diphosphooligosaccharide-protein glycotransferase / dolichyl-diphosphooligosaccharide-protein glycotransferase activity / rRNA primary transcript binding / maturation of 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / preribosome, small subunit precursor / protein glycosylation / preribosome, large subunit precursor / ribonucleoprotein complex binding / maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / cytosolic ribosome / rRNA processing / large ribosomal subunit / ribosome biogenesis / cytosolic large ribosomal subunit / RNA helicase activity / rRNA binding / RNA helicase / ribosome / structural constituent of ribosome / ribonucleoprotein complex / translation / nucleolus / ATP hydrolysis activity / RNA binding / nucleoplasm / ATP binding / membrane / metal ion binding / nucleus / cytoplasm
Similarity search - Function
Ribosomal RNA processing protein 1-like / Nucleolar protein,Nop52 / Mak16 protein / Mak16 protein C-terminal region / : / STT3/PglB/AglB core domain / Eukaryotic rRNA processing / Eukaryotic rRNA processing protein EBP2 / : / Ribosome biogenesis protein BRX1 ...Ribosomal RNA processing protein 1-like / Nucleolar protein,Nop52 / Mak16 protein / Mak16 protein C-terminal region / : / STT3/PglB/AglB core domain / Eukaryotic rRNA processing / Eukaryotic rRNA processing protein EBP2 / : / Ribosome biogenesis protein BRX1 / DDX18/Has1, DEAD-box helicase domain / Ribosome biogenesis protein Nop16 / Ribosome biogenesis protein Nop16 / Oligosaccharyl transferase, STT3 subunit / Oligosaccharyl transferase STT3, N-terminal / Domain of unknown function DUF4217 / Domain of unknown function (DUF4217) / DUF4217 / BOP1, N-terminal domain / WD repeat BOP1/Erb1 / BOP1NT (NUC169) domain / BOP1NT (NUC169) domain / U3 snoRNP protein/Ribosome production factor 1 / Pescadillo / Pescadillo N-terminus / Brix domain / Brix domain / Brix domain profile. / Brix / DEAD-box subfamily ATP-dependent helicases signature. / ATP-dependent RNA helicase DEAD-box, conserved site / RNA helicase, DEAD-box type, Q motif / Ribosomal L28e/Mak16 / DEAD-box RNA helicase Q motif profile. / Ribosomal L28e protein family / BRCA1 C Terminus (BRCT) domain / : / Ribosomal protein L23/L25, N-terminal / Ribosomal protein L35Ae, conserved site / Ribosomal protein L23, N-terminal domain / Ribosomal protein L13e, conserved site / Ribosomal protein L13e signature. / Ribosomal Protein L6, KOW domain / Ribosomal protein L13e / Ribosomal protein L13e / Ribosomal protein L14e domain / 60S ribosomal protein L6E / Ribosomal protein L35A / Ribosomal protein L36e / Ribosomal protein L36e domain superfamily / Ribosomal protein L36e / Ribosomal protein L35A superfamily / Ribosomal protein L7A/L8 / 60S ribosomal protein L4, C-terminal domain / 60S ribosomal protein L18a/ L20, eukaryotes / Ribosomal protein L7, eukaryotic / Ribosomal protein L6e / Ribosomal protein L14 / Ribosomal protein L30, N-terminal / Ribosomal protein L14, KOW motif / Ribosomal L30 N-terminal domain / Ribosomal protein L14 / Ribosomal protein L35Ae / Ribosomal protein L1, 3-layer alpha/beta-sandwich / 50S ribosomal protein L18Ae/60S ribosomal protein L20 and L18a / Ribosomal protein 50S-L18Ae/60S-L20/60S-L18A / 60S ribosomal protein L4 C-terminal domain / Ribosomal proteins 50S-L18Ae/60S-L20/60S-L18A / Ribosomal protein L6e signature. / Ribosomal protein L15e, conserved site / Ribosomal protein L21e / Ribosomal protein L21e, conserved site / Ribosomal protein L21 superfamily / Ribosomal protein 60S L18 and 50S L18e / Ribosomal protein L4/L1e, eukaryotic/archaeal, conserved site / Ribosomal protein L37e, conserved site / Ribosomal protein L21e / Ribosomal protein L36e signature. / Ribosomal protein L37e / Ribosomal_L15e / Ribosomal protein L15e / Ribosomal protein L15e core domain superfamily / Ribosomal protein L32e / Ribosomal protein L32e superfamily / Ribosomal_L32e / Ribosomal protein L37ae/L37e / Ribosomal protein L22/L17, eukaryotic/archaeal / Ribosomal protein L35Ae signature. / Ribosomal protein L18/L18-A/B/e, conserved site / Ribosomal protein L18e signature. / Ribosomal protein L26/L24, eukaryotic/archaeal / Ribosomal protein L13, eukaryotic/archaeal / Ribosomal protein L4, eukaryotic and archaeal type / Ribosomal protein L18e / Ribosomal protein L1-like / Ribosomal protein L1/ribosomal biogenesis protein / Ribosomal protein L37e / Ribosomal L15 / Ribosomal protein L32 / Ribosomal protein L1p/L10e family
Similarity search - Domain/homology
Brix domain-containing protein / 60S ribosomal protein L26-like protein / ATP-dependent RNA helicase / Ribosomal protein L15 / 60S ribosomal protein L6 / dolichyl-diphosphooligosaccharide--protein glycotransferase / 60S ribosomal protein l21-like protein / Ribosomal protein L37 / Putative RNA-binding protein / 60S ribosomal protein L14-like protein ...Brix domain-containing protein / 60S ribosomal protein L26-like protein / ATP-dependent RNA helicase / Ribosomal protein L15 / 60S ribosomal protein L6 / dolichyl-diphosphooligosaccharide--protein glycotransferase / 60S ribosomal protein l21-like protein / Ribosomal protein L37 / Putative RNA-binding protein / 60S ribosomal protein L14-like protein / Ribosomal RNA-processing protein 1 / Brix domain-containing protein / 60S ribosomal protein L25-like protein / 60S ribosomal protein L32-like protein / Uncharacterized protein / Nucleolar protein 16 / 60S ribosomal protein L13 / Ribosomal protein L18-like protein / 60S ribosomal protein L8 / 60S ribosomal protein L36 / 60S ribosomal protein L20 / Ribosome biogenesis protein ERB1 / 60S ribosomal protein l33-like protein / 60S ribosomal protein L4-like protein / Protein MAK16 / 60S ribosomal protein l7-like protein / rRNA processing protein / 60S ribosomal protein l17-like protein / 60S ribosomal protein L16-like protein / Pescadillo homolog
Similarity search - Component
Biological speciesChaetomium thermophilum (fungus)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.0 Å
AuthorsLau B / Huang Z / Beckmann R / Hurt E / Cheng J
Funding support1 items
OrganizationGrant numberCountry
Not funded
CitationJournal: EMBO Rep / Year: 2023
Title: Mechanism of 5S RNP recruitment and helicase-surveilled rRNA maturation during pre-60S biogenesis.
Authors: Benjamin Lau / Zixuan Huang / Nikola Kellner / Shuangshuang Niu / Otto Berninghausen / Roland Beckmann / Ed Hurt / Jingdong Cheng /
Abstract: Ribosome biogenesis proceeds along a multifaceted pathway from the nucleolus to the cytoplasm that is extensively coupled to several quality control mechanisms. However, the mode by which 5S ...Ribosome biogenesis proceeds along a multifaceted pathway from the nucleolus to the cytoplasm that is extensively coupled to several quality control mechanisms. However, the mode by which 5S ribosomal RNA is incorporated into the developing pre-60S ribosome, which in humans links ribosome biogenesis to cell proliferation by surveillance by factors such as p53-MDM2, is poorly understood. Here, we report nine nucleolar pre-60S cryo-EM structures from Chaetomium thermophilum, one of which clarifies the mechanism of 5S RNP incorporation into the early pre-60S. Successive assembly states then represent how helicases Dbp10 and Spb4, and the Pumilio domain factor Puf6 act in series to surveil the gradual folding of the nearby 25S rRNA domain IV. Finally, the methyltransferase Spb1 methylates a universally conserved guanine nucleotide in the A-loop of the peptidyl transferase center, thereby licensing further maturation. Our findings provide insight into the hierarchical action of helicases in safeguarding rRNA tertiary structure folding and coupling to surveillance mechanisms that culminate in local RNA modification.
History
DepositionFeb 7, 2023-
Header (metadata) releaseMay 17, 2023-
Map releaseMay 17, 2023-
UpdateJul 19, 2023-
Current statusJul 19, 2023Processing site: PDBc / Status: Released

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Structure visualization

Supplemental images

emd_35279.png

Downloads & links

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Map

FileDownload / File: emd_35279.map.gz / Format: CCP4 / Size: 282.6 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
Annotationlocal resolution filtered map
Voxel sizeX=Y=Z: 1.045 Å
Density
Contour LevelBy AUTHOR: 0.02
Minimum - Maximum-0.2242767 - 0.4486226
Average (Standard dev.)-0.000047342517 (±0.0075569814)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions420420420
Spacing420420420
CellA=B=C: 438.9 Å
α=β=γ: 90.0 °

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Supplemental data

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Additional map: cry-em map filtered by DeepEMhancer

Fileemd_35279_additional_1.map
Annotationcry-em map filtered by DeepEMhancer
Projections & Slices
AxesZYX

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Histogram

Histogram (log scale)

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Half map: #1

Fileemd_35279_half_map_1.map
Projections & Slices
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Histogram

Histogram (log scale)

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Half map: #2

Fileemd_35279_half_map_2.map
Projections & Slices
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Sample components

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Entire : pre-60S ribosome

EntireName: pre-60S ribosome
Components
  • Complex: pre-60S ribosome
    • Complex: ribosome
      • RNA: RNA (3341-MER)
      • RNA: RNA (306-MER)
      • Protein or peptide: Brix domain-containing protein
      • Protein or peptide: Ribosome biogenesis protein C8F11.04
      • Protein or peptide: Ribosome biogenesis protein ERB1
      • Protein or peptide: RNA helicaseHelicase
      • Protein or peptide: Putative RNA-binding protein
      • Protein or peptide: Pescadillo homolog
      • Protein or peptide: 60S ribosomal protein l7-like proteinRibosome
      • Protein or peptide: Nucleolar protein 16Nucleolus
      • Protein or peptide: rRNA-processing protein EBP2
      • Protein or peptide: 60S ribosomal protein L4-like proteinRibosome
      • Protein or peptide: 60S ribosomal protein L6
      • Protein or peptide: 60S ribosomal protein L8
      • Protein or peptide: 60S ribosomal protein L13
      • Protein or peptide: 60S ribosomal protein L14-like proteinRibosome
      • Protein or peptide: Ribosomal protein L15
      • Protein or peptide: 60S ribosomal protein L16-like proteinRibosome
      • Protein or peptide: 60S ribosomal protein l17-like proteinRibosome
      • Protein or peptide: Ribosomal protein L18-like proteinRibosome
      • Protein or peptide: 60S ribosomal protein L20
      • Protein or peptide: 60S ribosomal protein l21-like proteinRibosome
      • Protein or peptide: 60S ribosomal protein L25-like proteinRibosome
      • Protein or peptide: 60S ribosomal protein L26-like proteinRibosome
      • Protein or peptide: 60S ribosomal protein L32-like proteinRibosome
      • Protein or peptide: 60S ribosomal protein l33-like proteinRibosome
      • Protein or peptide: dolichyl-diphosphooligosaccharide--protein glycotransferase
      • Protein or peptide: 60S ribosomal protein L36
      • Protein or peptide: Ribosomal protein L37
      • Protein or peptide: Ribosomal RNA-processing protein 1
      • Protein or peptide: Brix domain-containing protein
      • Protein or peptide: Protein MAK16
  • Ligand: ZINC ION

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Supramolecule #1: pre-60S ribosome

SupramoleculeName: pre-60S ribosome / type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#32
Source (natural)Organism: Chaetomium thermophilum (fungus)

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Supramolecule #2: ribosome

SupramoleculeName: ribosome / type: complex / ID: 2 / Parent: 1 / Macromolecule list: #1-#32
Source (natural)Organism: Chaetomium thermophilum (fungus)

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Macromolecule #1: RNA (3341-MER)

MacromoleculeName: RNA (3341-MER) / type: rna / ID: 1 / Details: 25S rRNA / Number of copies: 1
Source (natural)Organism: Chaetomium thermophilum (fungus) / Strain: DSM 1495 / CBS 144.50 / IMI 039719
Molecular weightTheoretical: 1.0799771249999999 MDa
SequenceString: GGUUGACCUC GGAUCAGGUA GGAGGACCCG CUGAACUUAA GCAUAUCAAU AAGCGGAGGA AAAGAAACCA ACAGGGAUUG CCCUAGUAA CGGCGAGUGA AGCGGCAACA GCUCAAAUUU GAAAGCUGGC UUCGGCCCGC GUUGUAAUUU GGAGAGGAUG C UUUGGGCG ...String:
GGUUGACCUC GGAUCAGGUA GGAGGACCCG CUGAACUUAA GCAUAUCAAU AAGCGGAGGA AAAGAAACCA ACAGGGAUUG CCCUAGUAA CGGCGAGUGA AGCGGCAACA GCUCAAAUUU GAAAGCUGGC UUCGGCCCGC GUUGUAAUUU GGAGAGGAUG C UUUGGGCG AGGCUCCUUC UGAGUUCCCU GGAACGGGAC GCCACAGAGG GUGAGAGCCC CGUAUAGUUG GAAGCCAAGC CU GUGUAAA GCUCCUUCGA CGAGUCGAGU AGUUUGGGAA UGCUGCUCAA AAUGGGAGGU AAAUUUCUUC UAAAGCUAAA UAC CGGCCA GAGACCGAUA GCGCACAAGU AGAGUGAUCG AAAGAUGAAA AGCACUUUGA AAAGAGGGUU AAAUAGCACG UGAA AUUGU UGAAAGGGAA GCGCUUGUGA CCAGACUUGC GCCCGGCGGA UCAUCCGGUG UUCUCACCGG UGCACUCCGC CGGGC UCAG GCCAGCAUCG GUUCUGGCGG GGGGAUAAAG GCCCAGGGAA UGUGGCUCCU CCGGGAGUGU UAUAGCCCUG GGUGUA AUA CCCUCGCCGG GACCGAGGAC CGCGCUCUGC AAGGAUGCUG GCGUAAUGGU CACCAGCGAC CCGUCUUGAA ACACGGA CC AAGGAGUCAA GGUUUUGCGC GAGUGUUUGG GUGUAAAACC CGCACGCGUA AUGAAAGUGA ACGUAGGUGA GAGCUUCG G CGCAUCAUCG ACCGAUCCUG AUGUAUUCGG AUGGAUUUGA GUAGGAGCGU UAAGCCUUGG ACCCGAAAGA UGGUGAACU AUGCUUGGAU AGGGUGAAGC CAGAGGAAAC UCUGGUGGAG GCUCGCAGCG GUUCUGACGU GCAAAUCGAU CGUCAAAUCU GAGCAUGGG GGCGAAAGAC UAAUCGAACC AUCUAGUAGC UGGUUACCGC CGAAGUUUCC CUCAGGAUAG CAGUGUCGAC C UUCAGUUU UAUGAGGUAA AGCGAAUGAU UAGGGACUCG GGGGCGAUUU UUAGCCUUCA UCCAUUCUCA AACUUUAAAU AU GUAAGAA GCCCUUGUUA CUUAACUGAA CGUGGGCAUU CGAAUGUAUC GACACUAGUG GGCCAUUUUU GGUAAGCAGA ACU GGCGAU GCGGGAUGAA CCGAACGCGG GGUUAAGGUG CCGGAGUGGA CGCUCAUCAG ACACCACAAA AGGCGUUAGU ACAU CUUGA CAGCAGGACG GUGGCCAUGG AAGUCGGAAU CCGCUAAGGA CUGUGUAACA ACUCACCUGC CGAAUGUACU AGCCC UGAA AAUGGAUGGC GCUCAAGCGU CCCACCCAUA CCCCGCCCUC AGGGUAGAAA CGAUGCCCUG AGGAGUAGGC GGCCGU GGA GGUCAGUGAC GAAGCCUAGG GCGUGAGCCC GGGUCGAACG GCCUCUAGUG CAGAUCUUGG UGGUAGUAGC AAAUACU UC AAUGAGAACU UGAAGGACCG AAGUGGGGAA AGGUUCCAUG UGAACAGCGG UUGGACAUGG GUUAGUCGAU CCUAAGCC A UAGGGAAGUU CCGUUUCAAA GGGGCACUCG UGCCCCGUGU GGCGAAAGGG AAGCCGGUUA AUAUUCCGGC ACCUGGAUG UGGGUUUUGC GCGGCAACGC AACUGAACGC GGAGACGACG GCGGGGGCCC CGGGCAGAGU UCUCUUUUCU UCUUAACGGU CUAUCACCC UGGAAACAGU UUGUCUGGAG AUAGGGUUUA AUGGCCGGAA GAGCCCGACA CUUCUGUCGG GUCCGGUGCG C UCUCGACG UCCCUUGAAA AUCCGCGGGA GGGAAUAAUU CUCACGCCAG GUCGUACUCA UAACCGCAGC AGGUCCCCAA GG UGAACAG CCUCUGGUUG AUAGAACAAU GUAGAUAAGG GAAGUCGGCA AAAUAGAUCC GUAACUUCGG GAAAAGGAUU GGC UCUAAG GGUUGGGCAC GUUGGGCUUU GGGCGGACGC CCUGGGAGCA GAGGGCCUCU AGCCGGGCAA CCGGCCGGCG GCCC UCAGC ACCCGGGGUU GAAGCCCUUA GCAGGCUUCG GCCGUCCGGC GUGCGGUUAA CAACCAACUU AGAACUGGUA CGGAC AGGG GGAAUCUGAC UGUCUAAUUA AAACAUAGCA UUGCGAUGGC CAGAAAGUGG UGUUGACGCA AUGUGAUUUC UGCCCA GUG CUCUGAAUGU CAAAGUGAAG AAAUUCAACC AAGCGCGGGU AAACGGCGGG AGUAACUAUG ACUCUCUUAA GGUAGCC AA AUGCCUCGUC AUCUAAUUAG UGACGCGCAU GAAUGGAUUA ACGAGAUUCC CACUGUCCCU AUCUACUAUC UAGCGAAA C CACAGCCAAG GGAACGGGCU UGGCAAAAUC AGCGGGGAAA GAAGACCCUG UUGAGCUUGA CUCUAGUUUG ACAUUGUGA AAAGACAUAG GAGGUGUAGA AUAGGUGGGA GCUUCGGCGC CAGUGAAAUA CCACUACUCC UAUUGUUUUU UUACUUAUUC AAUGAAGCG GGGCUGGACU UGCGUCCAAC UUCUGGAGUU AAGGUCCUUC GCGGGCCGAC CCGGGUUGAA GACAUUGUCA G GUGGGGAG UUUGGCUGGG GCGGCACAUC UGUUAAACCA UAACGCAGGU GUCCUAAGGG GGGCUCAUGG AGAACAGAAA UC UCCAGUA GAACAAAAGG GUAAAAGUCC CCUUGAUUUU GAUUUUCAGU GUGAAUACAA ACCAUGAAAG UGUGGCCUAU CGA UCCUUU AGUCCCUCGA AAUUUGAGGC UAGAGGUGCC AGAAAAGUUA CCACAGGGAU AACUGGCUUG UGGCGGCCAA GCGU UCAUA GCGACGUCGC UUUUUGAUCC UUCGAUGUCG GCUCUUCCUA UCAUACCGAA GCAGAAUUCG GUAAGCGUUG GAUUG UUCA CCCACUAAUA GGGAACGUGA GCUGGGUUUA GACCGUCGUG AGACAGGUUA GUUUUACCCU ACUGAUGAAC UCGUCG CAA UGGUAAUUCA GCUUAGUACG AGAGGAACCG CUGAUUCAGA UAAUUGGUUU UUGCGGUUGU CCGACCGGGC AGUGCCG CG AAGCUACCAU CUGCUGGAUA AUGGCUGAAC GCCUCUAAGU CAGAAUCCAU GCCAGAACGC GACGAUACUA CCCGCACG U UGUAGACGUA UAAGAAUAGG CUCCGGCCUC GUAUCCUAGC AGGCGAUUCC UCCGCCGGCC UCGAAGUGGC CGUCGGUAA UUCGCGUAUU GCAAUUUAGA CACGCGCGGG AUCAAAUCCU UUGCAGACGA CUUAGAUGUG CGAAAGGGUC CUGUAAGCAG UAGAGUAGC CUUGUUGUUA CGAUCUGCUG AGGGUAAGCC CUCCUUCGCC UAGAUUUCCC AG

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Macromolecule #2: RNA (306-MER)

MacromoleculeName: RNA (306-MER) / type: rna / ID: 2 / Details: 5.8S rRNA / Number of copies: 1
Source (natural)Organism: Chaetomium thermophilum (fungus) / Strain: DSM 1495 / CBS 144.50 / IMI 039719
Molecular weightTheoretical: 98.523078 KDa
SequenceString: AAACUUUCAA CAACGGAUCU CUUGGUUCUG GCAUCGAUGA AGAACGCAGC GAAAUGCGAU AAGUAAUGUG AAUUGCAGAA UUCCGUGAA UCAUCGAAUC UUUGAACGCA CAUUGCGCCC GCCGGUAUUC CGGCGGGCAU GCCUGUUCGA GCGUCAUUUC A ACCAUCAA ...String:
AAACUUUCAA CAACGGAUCU CUUGGUUCUG GCAUCGAUGA AGAACGCAGC GAAAUGCGAU AAGUAAUGUG AAUUGCAGAA UUCCGUGAA UCAUCGAAUC UUUGAACGCA CAUUGCGCCC GCCGGUAUUC CGGCGGGCAU GCCUGUUCGA GCGUCAUUUC A ACCAUCAA GCCCUGGGCU UGUGUUGGGG ACCCGCGGCU GCUCGCGGGC CCUGAAAAGC AGUGGCGGGC UCGCUAGUCA CA CCGAGCG UAGUAGAACU UCUAUUCGAU CUCGCUCAGG GCGUGCGGCG GGUGCCAGCC GUAAAACCC

GENBANK: GENBANK: GQ922527.1

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Macromolecule #3: Brix domain-containing protein

MacromoleculeName: Brix domain-containing protein / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chaetomium thermophilum (fungus) / Strain: DSM 1495 / CBS 144.50 / IMI 039719
Molecular weightTheoretical: 36.48202 KDa
SequenceString: MAAVYKTIAK VTGSKEDKDK EDRSNIIAER KNKQRVLVLS SRGVTYRHRH LLNDLASMLP HGRKDAKFDT KSRLYELCEL AELYNCNNV LFFEARKGKD LYMWFSKVPN GPTVKFYAQN LHTMEELHFQ GNCLKGSRPI LSFDAAFEQE PYLKVIKELF L HTFGVPQG ...String:
MAAVYKTIAK VTGSKEDKDK EDRSNIIAER KNKQRVLVLS SRGVTYRHRH LLNDLASMLP HGRKDAKFDT KSRLYELCEL AELYNCNNV LFFEARKGKD LYMWFSKVPN GPTVKFYAQN LHTMEELHFQ GNCLKGSRPI LSFDAAFEQE PYLKVIKELF L HTFGVPQG HKKSKPFIDH VLSFSVADGK IWVRNYEIRE VEKVKGEADK REEGETEKPK AAKPGSKDTD INLIEIGPRF VL TPIIIQE GSFGGPILYE NKRFISPNKI RAELRKAKAA RHHARMEQQR DLLARKRQLG LDESTPKKKD DLDTRELFA

UniProtKB: Brix domain-containing protein

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Macromolecule #4: Ribosome biogenesis protein C8F11.04

MacromoleculeName: Ribosome biogenesis protein C8F11.04 / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chaetomium thermophilum (fungus) / Strain: DSM 1495 / CBS 144.50 / IMI 039719
Molecular weightTheoretical: 43.700527 KDa
SequenceString: MAPSTAVAKK ETDAVIPVDP DQTLKACKAL LAHIKKAAAA PRPDGKQNLL ADEESTVAET PIWLTLTTKK HIHDSHRLQP GKIILPHPL NTSEEISVCL ITADPQRFYK NAVADEFPED LRAKIGRVID ISHLKAKFKA YEAQRKLFSE HDVFLADTRI I NRLPKALG ...String:
MAPSTAVAKK ETDAVIPVDP DQTLKACKAL LAHIKKAAAA PRPDGKQNLL ADEESTVAET PIWLTLTTKK HIHDSHRLQP GKIILPHPL NTSEEISVCL ITADPQRFYK NAVADEFPED LRAKIGRVID ISHLKAKFKA YEAQRKLFSE HDVFLADTRI I NRLPKALG KTFYKTTTKR PIPVVLMAQR EKVNGKRVPA PKGKKEKRDP LENANARPIP EIVAEIRKAI GAALVHLSPS TN TAIKVGY ANWEPEKLAA NIETVIRELV ERFVPQKWQN VRNFYVKGPE TAALPIYQTD ELWLDESKVV PDGQEPARAL PGK REKANI GKKRKPLEDA SQPALEETGK DERPKKKAKK TLPESNDDKL DKAIAERKEQ LKKQKAAAKK VAADI

UniProtKB: Uncharacterized protein

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Macromolecule #5: Ribosome biogenesis protein ERB1

MacromoleculeName: Ribosome biogenesis protein ERB1 / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chaetomium thermophilum (fungus) / Strain: DSM 1495 / CBS 144.50 / IMI 039719
Molecular weightTheoretical: 90.838023 KDa
SequenceString: MGSKIVEKKR KSRDSDSESD NELGDGLFDG VLSQSEDEED YIPSSEVDED DDDDADESAS EDSDDSNDSE DDEVEEDDEA LLSDEIPSE GESEKDQDLA ESKESKQDQD KEPSEPEILE PFVDPPRKED EELEDRNYRI EKDANGGIRY VYDEIDPVYD S DDTDYNVP ...String:
MGSKIVEKKR KSRDSDSESD NELGDGLFDG VLSQSEDEED YIPSSEVDED DDDDADESAS EDSDDSNDSE DDEVEEDDEA LLSDEIPSE GESEKDQDLA ESKESKQDQD KEPSEPEILE PFVDPPRKED EELEDRNYRI EKDANGGIRY VYDEIDPVYD S DDTDYNVP VNTIGNIPLS FYDSYPHIGY DINGKKIMRP ATGDALQNLL DSIEVPEGWT GLTDPNTGKP LNLSRDELEL IR KVQQGLI PDDVEDPYPD TVEWFTSVEE KMPLSAAPEP KRRFIPSKNE AKQIMKLVRA IREGRILPYK PPEEREREEL EKE EEFYDL WQNEEPQPPN PMHIPAPKLP PPGYDLSYNP PPEYLPTKEE REEWEKMDPE DREKDYLPTK YDSLRKVPAW GNFV KERFE RCMDLYLAPR VRKNRLNIDP NSLLPKLPSP DELKPFPTVQ QTIFRGHEGR VRSVAIDPTG VALATGGDDG TVRVW ELLT GRQVWSVKLN GDEAVNTVRW RPTKDTFILA AAAGEDIFLM IPTHPSVTPA LDQASRDILN AGFGHATNGK QQANLP PGK EPPGKWARPG TRLEDEGVLL RITVRSTIKA ISWHRRGDHF ATVSPSGQRS SVAIHTLSKH LTQIPFRKLN GLAQTAS FH PLRPLFFVAT QRSIRCYDLQ KLELVKIVQP GAKWISSFDV HPGGDNLVVG SYDKRLLWHD LDLSNRPYKT MRFHTEAI R AVRFHKGGLP LFADASDDGS LQIFHGKVPN DQLENPTIVP VKMLKGHKVV NKLGVLDIDW HPREPWCVSA GADGTARLW M

UniProtKB: Ribosome biogenesis protein ERB1

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Macromolecule #6: RNA helicase

MacromoleculeName: RNA helicase / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chaetomium thermophilum (fungus) / Strain: DSM 1495 / CBS 144.50 / IMI 039719
Molecular weightTheoretical: 66.830867 KDa
SequenceString: MASESSKKRK FKDSKGPKPD EAVAKPSKFK KLKRDPTPEE RETSDSESGN SDGQHEPDQV DSDGEDAVSD DGSFDEKVAH QDEDGSRPE NGTDLEDVPK NDNLLPPPTN AQEFSELNLS EKTTKAIAEM GFTKMTEIQR RAIPPALAGK DVLGAAKTGS G KTLAFLIP ...String:
MASESSKKRK FKDSKGPKPD EAVAKPSKFK KLKRDPTPEE RETSDSESGN SDGQHEPDQV DSDGEDAVSD DGSFDEKVAH QDEDGSRPE NGTDLEDVPK NDNLLPPPTN AQEFSELNLS EKTTKAIAEM GFTKMTEIQR RAIPPALAGK DVLGAAKTGS G KTLAFLIP AVEMLSSLRF KPRNGTGAIV VTPTRELALQ IFGVARELMK YHSQTYGVVI GGANRRAEAE KLGKGVNLLI AT PGRLLDH LQNTPFVFKN LKSLIIDEAD RILEIGFEDE MRQIVKILPK EDRQTMLFSA TQTTKVEDLA RISLRPGPLY INV DEEKKY STVEGLEQGY VVVEADKRFL LLFSFLKKMA KKKIIVFFSS CNSVKYYSEL LQYIDLPVLD LHGKQKQQKR TNTF FEFCN AKSGTLICTD VAARGLDIPQ VDWIVQFDPP DDPRDYIHRV GRTARGNNGK GRSLLFLQPC ELGFLAHLKA AKVPV VEYD FPKNKILNVQ SQLEKLISTN YYLNQSAKEG YRSYIHAYAS HSLRSVFDVH KLDLVKVAKS FGFSTPPRVD ITLSAS LSR DKKPQGRRAY GSQPRQGGRY KKVGDIPDPQ PNTFG

UniProtKB: ATP-dependent RNA helicase

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Macromolecule #7: Putative RNA-binding protein

MacromoleculeName: Putative RNA-binding protein / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chaetomium thermophilum (fungus) / Strain: DSM 1495 / CBS 144.50 / IMI 039719
Molecular weightTheoretical: 45.884414 KDa
SequenceString: MAAELRKKKS KSAVADADAA ISKKEKATKV TKATPKAEKR KAPEEASPVA IKKQKADKDA VVKKAAVAAE DKIAEKPVKS VLKKETSAK AKEAAKPKKK QEKVEEEEEE ALENADAAFG SDEESELDEQ TKALMDTLDD GEESDEDESK QVSTFKKGQD V GKIPTPKK ...String:
MAAELRKKKS KSAVADADAA ISKKEKATKV TKATPKAEKR KAPEEASPVA IKKQKADKDA VVKKAAVAAE DKIAEKPVKS VLKKETSAK AKEAAKPKKK QEKVEEEEEE ALENADAAFG SDEESELDEQ TKALMDTLDD GEESDEDESK QVSTFKKGQD V GKIPTPKK APQKQTNGDS KPAGDSQPGV MYIARLPHGF YEHELRGYFS QFGEITRLRV VRNKKTGASR HRAFIEFADA EV ADIAART MDKYLLFGHI LTCKIVPPAQ VHPDLFKGAN RRFKVVPWNK MAGRQLERPL SESQWQVKVA KEEQRRAARA EKL KEMGYE FEAPALKVPK AKPVLENGEE EKKAIETAPA PAVEEKESKE VAKRQAEEEV EEKAPAPKTK KVKEDKPAKE AATI SAPKK GKKSKKTKS

UniProtKB: Putative RNA-binding protein

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Macromolecule #8: Pescadillo homolog

MacromoleculeName: Pescadillo homolog / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chaetomium thermophilum (fungus) / Strain: DSM 1495 / CBS 144.50 / IMI 039719
Molecular weightTheoretical: 77.16725 KDa
SequenceString: MGKAKKKGKS GAARNYMTRT QAVKKLQLSL PDFRKLCIWK GIYPREPRDR RKVNKSATAS TTFYYTKDIQ YLLHEPLLQK FREQKALEK KISRALGRGD VSNAARLERN ANLPEKTGKP RYTLNHIIRE RYPTFQDALR DLDDCLSMLF LFANLPSTTA V PAKMIARC ...String:
MGKAKKKGKS GAARNYMTRT QAVKKLQLSL PDFRKLCIWK GIYPREPRDR RKVNKSATAS TTFYYTKDIQ YLLHEPLLQK FREQKALEK KISRALGRGD VSNAARLERN ANLPEKTGKP RYTLNHIIRE RYPTFQDALR DLDDCLSMLF LFANLPSTTA V PAKMIARC ERLCHEFQHY LIVTHSLRKS FLSIKGIYYQ ANIQGEDILW LVPYKFNQRI VGDVDFRIMG TFVEFYMTLL GF VNYRLYT SIGLKYPPKF DQVKDDQGAE LAAFSLEGLN LASQNGEQKA ITNGEEHGPD PKVQAEVDKL VAKLREEEQQ ANG DKTDEK GEENEGDDKP SDAIDKFEPV APGGDVLPQP SYSSSDPSQL FANFTFFLSR ETPRQPLEFI LRAFGCKRIG WDAV LGEGA FTTDESDPRI THQIIDRPVI RAAVSEDGDG EDNQTSQKLA PNGRYPGRIY VQPQWVWDSI NDEELKPPEL YAPGA QLPP HLSPFVKPTQ GQYDPTKPLE EQQTEAEALE AELEDAQAQQ EGSDEESGSE VENDMSVASD EEDEQEDDFG GFSDED EEQ SDEGSEEGDE EEDDDEEEEA TLERQRELEA ELAGKAVSKG KPLDPKVKAK LEAKKALERK KKQEAEELER AKGMLSK KK RKLFEQMQYS NAKKNAEDAK LRAKRRRIEK EMAAKKA

UniProtKB: Pescadillo homolog

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Macromolecule #9: 60S ribosomal protein l7-like protein

MacromoleculeName: 60S ribosomal protein l7-like protein / type: protein_or_peptide / ID: 9 / Number of copies: 2 / Enantiomer: LEVO
Source (natural)Organism: Chaetomium thermophilum (fungus) / Strain: DSM 1495 / CBS 144.50 / IMI 039719
Molecular weightTheoretical: 28.821816 KDa
SequenceString: MSSTTVPTQN DILVPETLLK KRKSQEKARA ERAAALEKRK QANKEKRQVI FKRAEKYVKE YREQEREKIR LARIAKQQGS FHIPAEAKL VFVIRIKGIN KIPPKPRKIL QLLRLRQINN GVFVKVTKAT AEMIKIVEPW VAYGYPNLKS VRELIYKRGY G KVNGQRIP ...String:
MSSTTVPTQN DILVPETLLK KRKSQEKARA ERAAALEKRK QANKEKRQVI FKRAEKYVKE YREQEREKIR LARIAKQQGS FHIPAEAKL VFVIRIKGIN KIPPKPRKIL QLLRLRQINN GVFVKVTKAT AEMIKIVEPW VAYGYPNLKS VRELIYKRGY G KVNGQRIP LTDNAIIEEN LGKYGIICIE DLIHEIFTVG PNFKQAANFL WPFKLSNPNG GFRPRKFKHF IEGGDLGNRE EH INALIRA MN

UniProtKB: 60S ribosomal protein l7-like protein

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Macromolecule #10: Nucleolar protein 16

MacromoleculeName: Nucleolar protein 16 / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chaetomium thermophilum (fungus) / Strain: DSM 1495 / CBS 144.50 / IMI 039719
Molecular weightTheoretical: 26.960477 KDa
SequenceString: MGRELQKRKR RSSRPTIRMP NRRKKALNPA GNNIIAKSWN KKETLSQNYT RFGLVAKLGK ATGGTAPGNK ALLKDLNDPL AIKSGDQGL IKVSEVKVER DEQGRIKRVV RDNNPLNDPL NDLDSDSESD AVPQQQQQEQ PVLNNHIRQH DLELENVAAD V DDESKPEV ...String:
MGRELQKRKR RSSRPTIRMP NRRKKALNPA GNNIIAKSWN KKETLSQNYT RFGLVAKLGK ATGGTAPGNK ALLKDLNDPL AIKSGDQGL IKVSEVKVER DEQGRIKRVV RDNNPLNDPL NDLDSDSESD AVPQQQQQEQ PVLNNHIRQH DLELENVAAD V DDESKPEV LRALEREATR PVEKTVRHQS EREREWLQRL VDKHGDDVAA MARDRKLNPY QQTASDIKRR LKKAGLLSA

UniProtKB: Nucleolar protein 16

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Macromolecule #11: rRNA-processing protein EBP2

MacromoleculeName: rRNA-processing protein EBP2 / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chaetomium thermophilum (fungus) / Strain: DSM 1495 / CBS 144.50 / IMI 039719
Molecular weightTheoretical: 50.255562 KDa
SequenceString: MAKKGATQSP KAGSKPNSQT GDAQSKNQKA DRRKSNEPVV EKVAEKEEQR EEETEESEED ESEDEQLQIR ANGNNESDSE EDSDENSED EDESDDDSGD EHPTHHIDFE AIDDSDSESE LDSDEENDKK QDSNKAQNGM SEDKMDVDKD GEDKSGDEED E EEEIDVEE ...String:
MAKKGATQSP KAGSKPNSQT GDAQSKNQKA DRRKSNEPVV EKVAEKEEQR EEETEESEED ESEDEQLQIR ANGNNESDSE EDSDENSED EDESDDDSGD EHPTHHIDFE AIDDSDSESE LDSDEENDKK QDSNKAQNGM SEDKMDVDKD GEDKSGDEED E EEEIDVEE LEPEDEEDEE QIAAGTRTRQ TINNKEGLLT ALRRIQLDIN PKTTPFSFHQ SVVASKRTED SIPQIEDDLQ RE LAFMNQA LEAARLGRSL LRKEGVPFTR PTDYFAEMVR TDATMEKVKQ KLIEEATAKK AAAEARKQRD LKKFGKAVQV QKQ LERAKQ KREALDKINQ LKRKRAEGGS AALGATEADD PFDVAVDNEL SRDSKPKKRR ADGDHPVPNP KRQKKNAKYG FGGK KRGSK SGDAISSGDL SGFSVKRMKY GGGAGGGKKK ANRPGKARRK AAAAKR

UniProtKB: rRNA processing protein

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Macromolecule #12: 60S ribosomal protein L4-like protein

MacromoleculeName: 60S ribosomal protein L4-like protein / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chaetomium thermophilum (fungus) / Strain: DSM 1495 / CBS 144.50 / IMI 039719
Molecular weightTheoretical: 39.439938 KDa
SequenceString: MASRPTVTVF GADGKPTGAT EVLPKVFSAP IRPDIVKHVH TGMAKNKRQP YAVSEKAGHQ TSAESWGTGR AVARIPRVSG GGTHRAGQG AFGNMCRSGR MFAPTKIWRK WHVKINQGQK RFATASALAA SAVAPLLMAR GHQVSTVPEV PLVVDSAAVA G DAVAKTAA ...String:
MASRPTVTVF GADGKPTGAT EVLPKVFSAP IRPDIVKHVH TGMAKNKRQP YAVSEKAGHQ TSAESWGTGR AVARIPRVSG GGTHRAGQG AFGNMCRSGR MFAPTKIWRK WHVKINQGQK RFATASALAA SAVAPLLMAR GHQVSTVPEV PLVVDSAAVA G DAVAKTAA AYKLLKAIGA GPDVEKVKKS KKLRAGKGKM RGRRHRQRRG PLIVYSPEHD GKELVKGFRN IPGVETCPVD AL NLLQLAP GGHLGRFIVW TSAAIKQLDA VYESKKGFFL PANIVSQADL SRLINSTEIQ SVLRAPKGEA RTKRACVQKK NPL RNKQIM LRLNPYASTF AKEKLGEVKA EEGKPPKVPA SFKELLHEA

UniProtKB: 60S ribosomal protein L4-like protein

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Macromolecule #13: 60S ribosomal protein L6

MacromoleculeName: 60S ribosomal protein L6 / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chaetomium thermophilum (fungus) / Strain: DSM 1495 / CBS 144.50 / IMI 039719
Molecular weightTheoretical: 22.132994 KDa
SequenceString: MSAAPTTKTF GKGTRTVPAP SEKAQKWYPA EDEAQPKKVR KAVRPWTPRK SLQPGTVLIL LAGRFRGKRV VLLKCLDQGV LLVTGPFKI NGVPLRRVNA RYVIATSVKV DLTGVDQAKI DEVAQPKYFT AEKAKEKASE EAFFKQGEKP QKKPVSSTRA A DQKAIDKA ...String:
MSAAPTTKTF GKGTRTVPAP SEKAQKWYPA EDEAQPKKVR KAVRPWTPRK SLQPGTVLIL LAGRFRGKRV VLLKCLDQGV LLVTGPFKI NGVPLRRVNA RYVIATSVKV DLTGVDQAKI DEVAQPKYFT AEKAKEKASE EAFFKQGEKP QKKPVSSTRA A DQKAIDKA LIANIKKVDM LASYLASSFS LRKGDKPHLM KF

UniProtKB: 60S ribosomal protein L6

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Macromolecule #14: 60S ribosomal protein L8

MacromoleculeName: 60S ribosomal protein L8 / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chaetomium thermophilum (fungus) / Strain: DSM 1495 / CBS 144.50 / IMI 039719
Molecular weightTheoretical: 29.74449 KDa
SequenceString: MPPKSGKKVA PAPFPQGKAG AKKAPKNPLL EKRPRNFGIG QDIQPKRNLS RMVKWPEYIR LQRQKKILRM RLKVPPAIAQ FQYTLDKNL AAQAFKLLNK YRPETKQEKK ERLLREATAI KEGKKKEDVS KKPYTVKYGL NHVVGLIENK KASLVLIAND V DPIELVVF ...String:
MPPKSGKKVA PAPFPQGKAG AKKAPKNPLL EKRPRNFGIG QDIQPKRNLS RMVKWPEYIR LQRQKKILRM RLKVPPAIAQ FQYTLDKNL AAQAFKLLNK YRPETKQEKK ERLLREATAI KEGKKKEDVS KKPYTVKYGL NHVVGLIENK KASLVLIAND V DPIELVVF LPALCRKMGI PYAIIKGKAR LGTLVHKKTA AVVAITEVRS EDKNELAKLI SAVKEGYLEK VEDTRKRWGG GI MGFKAQK REEKRKKSLE TAIKV

UniProtKB: 60S ribosomal protein L8

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Macromolecule #15: 60S ribosomal protein L13

MacromoleculeName: 60S ribosomal protein L13 / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chaetomium thermophilum (fungus) / Strain: DSM 1495 / CBS 144.50 / IMI 039719
Molecular weightTheoretical: 24.19118 KDa
SequenceString: MAIKHNQQIP NNHFRKHWQR RVRCHFDQPG KKVTRRLARR AKAAALAPRP VDKLRPIVRC PTVKYNRRTR LGRGFTLEEL KAAGIPRLL APTIGIAVDH RRKNLSEESL AANVQRLKDY KARLILFPRK SNKPKKADTP KDQQTAETTT SLRTSFGVEQ P LAPGFTEI ...String:
MAIKHNQQIP NNHFRKHWQR RVRCHFDQPG KKVTRRLARR AKAAALAPRP VDKLRPIVRC PTVKYNRRTR LGRGFTLEEL KAAGIPRLL APTIGIAVDH RRKNLSEESL AANVQRLKDY KARLILFPRK SNKPKKADTP KDQQTAETTT SLRTSFGVEQ P LAPGFTEI SKSEIPAGIE GGAYRALRKA RSDARLVGVR EKRAKEKAEA EANKK

UniProtKB: 60S ribosomal protein L13

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Macromolecule #16: 60S ribosomal protein L14-like protein

MacromoleculeName: 60S ribosomal protein L14-like protein / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chaetomium thermophilum (fungus) / Strain: DSM 1495 / CBS 144.50 / IMI 039719
Molecular weightTheoretical: 16.111901 KDa
SequenceString:
MAEINVEATS WRRVEVGRVL KLENGSLAAI VEIIDHKRVL ADGPSSDPKL ATPRGIVPLS RALLTPIVIP KLPRGARTGA VKKAWEAYG VDAKWKETNW AKKQLQQERR QSLTDFDRFK VMRLKKQRRF EERKALAKIK AAA

UniProtKB: 60S ribosomal protein L14-like protein

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Macromolecule #17: Ribosomal protein L15

MacromoleculeName: Ribosomal protein L15 / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chaetomium thermophilum (fungus) / Strain: DSM 1495 / CBS 144.50 / IMI 039719
Molecular weightTheoretical: 24.307287 KDa
SequenceString: MGALKYLEEL SKKKQSDVVR FLLRVRCWEY RQLNVIHRAS RPSRPDKARR LGYKAKQGYV IYRVRVRRGG RKRPVPKGAT YGKPTNQGV NQLKYQRSLR ATAEERVGRR CSNLRVLNSY WVNQDSTYKY YEVILVDPNH KAIRRDPRIN WICNPVHKHR E CRGLTSTG ...String:
MGALKYLEEL SKKKQSDVVR FLLRVRCWEY RQLNVIHRAS RPSRPDKARR LGYKAKQGYV IYRVRVRRGG RKRPVPKGAT YGKPTNQGV NQLKYQRSLR ATAEERVGRR CSNLRVLNSY WVNQDSTYKY YEVILVDPNH KAIRRDPRIN WICNPVHKHR E CRGLTSTG KKSRGLNKGH RFNKTRAGRR KTWKRHNTLS LWRYR

UniProtKB: Ribosomal protein L15

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Macromolecule #18: 60S ribosomal protein L16-like protein

MacromoleculeName: 60S ribosomal protein L16-like protein / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chaetomium thermophilum (fungus) / Strain: DSM 1495 / CBS 144.50 / IMI 039719
Molecular weightTheoretical: 22.993195 KDa
SequenceString: MSSFESVVVI DGKGHLLGRL ASIVAKQLLS GQKIVVVRCE ALNISGEFFR AKLKYHAYLR KMTRYNPTRG GPFHFRAPSR IFYKAVRGM IPHKTARGAA ALERLKVFEG VPPPYDKKKK MVVPQALRVL RLQPGRKYCT VGRLSHEVGW KYQDVVARLE E RRKAKGAA ...String:
MSSFESVVVI DGKGHLLGRL ASIVAKQLLS GQKIVVVRCE ALNISGEFFR AKLKYHAYLR KMTRYNPTRG GPFHFRAPSR IFYKAVRGM IPHKTARGAA ALERLKVFEG VPPPYDKKKK MVVPQALRVL RLQPGRKYCT VGRLSHEVGW KYQDVVARLE E RRKAKGAA YYERKKLAAR QLSEAKKAAA AKVDPKVSEA LAAYGY

UniProtKB: 60S ribosomal protein L16-like protein

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Macromolecule #19: 60S ribosomal protein l17-like protein

MacromoleculeName: 60S ribosomal protein l17-like protein / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chaetomium thermophilum (fungus) / Strain: DSM 1495 / CBS 144.50 / IMI 039719
Molecular weightTheoretical: 21.072207 KDa
SequenceString:
MVRYAATEIA PTKSARARGS YLRVSFKNTR ETAQAINGWK LQRALTFLQN VIDKKEAVPM RRYAGSTGRT AQGKQWGVSR ARWPVKSAQ FLIGLLKNAE ANADAKGLDT GNLIIKHIQV NQAPKQRRRT YRAHGRINPY MSNPCHIELI LTEAEETVQK S EAVVRDVE SHLSSRQRGV RIRRALTAA

UniProtKB: 60S ribosomal protein l17-like protein

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Macromolecule #20: Ribosomal protein L18-like protein

MacromoleculeName: Ribosomal protein L18-like protein / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chaetomium thermophilum (fungus) / Strain: DSM 1495 / CBS 144.50 / IMI 039719
Molecular weightTheoretical: 24.195535 KDa
SequenceString: MDLVCAFIGP LFAPGARRLN LEEIAAESSS GIDLRWKHVR SSHRKAPKSD NVYLKLLVKL YRFLARRTEA PFNKVVLRRL FMSRINRPP VSLSRIARNL KNGNEKKTVV VVATVTDDNR LLQVPKMEVA ALRFTAKARA RIEAAGGRTL TLDQLALEKP T GANTLLLR ...String:
MDLVCAFIGP LFAPGARRLN LEEIAAESSS GIDLRWKHVR SSHRKAPKSD NVYLKLLVKL YRFLARRTEA PFNKVVLRRL FMSRINRPP VSLSRIARNL KNGNEKKTVV VVATVTDDNR LLQVPKMEVA ALRFTAKARA RIEAAGGRTL TLDQLALEKP T GANTLLLR GPKNAREAVK HFGFGPHKHK KPYVRSKGRK FERARGRRRS RGFKV

UniProtKB: Ribosomal protein L18-like protein

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Macromolecule #21: 60S ribosomal protein L20

MacromoleculeName: 60S ribosomal protein L20 / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chaetomium thermophilum (fungus) / Strain: DSM 1495 / CBS 144.50 / IMI 039719
Molecular weightTheoretical: 20.341893 KDa
SequenceString:
MGRLQEYQVI GRHLPTEANP NPALYRMRIF APNEVVAKSR FWYFLRGLKK VKKATGEIVS VNVISEKHPL KVKNFGIWLR YDSRSGTHN MYKEYRETSR VAAVEAMYAD MAARHRARFR SIHILKVVEL EKTEDVKRPY IKQLVAKNLS FPLPHRVPKI S TKKIFSAK RPSTFS

UniProtKB: 60S ribosomal protein L20

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Macromolecule #22: 60S ribosomal protein l21-like protein

MacromoleculeName: 60S ribosomal protein l21-like protein / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chaetomium thermophilum (fungus) / Strain: DSM 1495 / CBS 144.50 / IMI 039719
Molecular weightTheoretical: 18.159227 KDa
SequenceString:
MGHAAGLRSG TRYAFSRGFR KHGQIPLSTY LRTYRVGDIV DIKVNGAVQK GMPHKFYHGK TGVVYNVTKS AVGVIVYKRV KHRYIEKRI NVRIEHVKPS RSREDFLRRV KENAELKKKA KAEGVPVQLK RQPAMPREAH TVSIADNKPV TLAPVAYETT I

UniProtKB: 60S ribosomal protein l21-like protein

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Macromolecule #23: 60S ribosomal protein L25-like protein

MacromoleculeName: 60S ribosomal protein L25-like protein / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chaetomium thermophilum (fungus) / Strain: DSM 1495 / CBS 144.50 / IMI 039719
Molecular weightTheoretical: 17.175334 KDa
SequenceString:
MAPKDVKKGG ASKAAKGAQA KKAAQAALKG VHSHKKTKVR KSTTFHRPKT LVLSRAPKYP RKSIPHEPRL DEHKIIIHPL NTEGALKKI EEQNTLVFIV DVKANKAQIK QALKKLYDID TVKINTLIRP DGTKKAFARL TPDVDALDIA ATKLGLV

UniProtKB: 60S ribosomal protein L25-like protein

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Macromolecule #24: 60S ribosomal protein L26-like protein

MacromoleculeName: 60S ribosomal protein L26-like protein / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chaetomium thermophilum (fungus) / Strain: DSM 1495 / CBS 144.50 / IMI 039719
Molecular weightTheoretical: 15.565322 KDa
SequenceString:
MKVRPTVSSS RRKARKAHFS APSSVRRVIM SAPLSKELRE KYNVRSIPIR KGDEVQIVRG AHKDKEGKVT SVYRLKYVIH VERVTREKA TGQTVPIGIH PSNVVITKLH LDKDRENILA RIKAGREQVA KAKGKKTAA

UniProtKB: 60S ribosomal protein L26-like protein

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Macromolecule #25: 60S ribosomal protein L32-like protein

MacromoleculeName: 60S ribosomal protein L32-like protein / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chaetomium thermophilum (fungus) / Strain: DSM 1495 / CBS 144.50 / IMI 039719
Molecular weightTheoretical: 15.095985 KDa
SequenceString:
MVAAKKHVPI VKKRTKRFMR HQSDRFKCLD SAWRKPKGID NRVRRRFKGN LAMPSIGYGS NKKTKHMMPS GHKAFLVHNV KDVELLLMH NRTYAAEIAH NVSSRKRIDI ITRAKQLGVK VTNAKAKVTT EV

UniProtKB: 60S ribosomal protein L32-like protein

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Macromolecule #26: 60S ribosomal protein l33-like protein

MacromoleculeName: 60S ribosomal protein l33-like protein / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chaetomium thermophilum (fungus) / Strain: DSM 1495 / CBS 144.50 / IMI 039719
Molecular weightTheoretical: 12.330337 KDa
SequenceString:
MPSEAGHRLY VKGRHLSYQR GRRNTHPKTS LIKIEGVDDT AAANFYLGKR VAYVYRAQKE VRGTKIRVIW GKITRPHGNS GVVRAKFTH PLPARSFGAS VRIMLYPSSI

UniProtKB: 60S ribosomal protein l33-like protein

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Macromolecule #27: dolichyl-diphosphooligosaccharide--protein glycotransferase

MacromoleculeName: dolichyl-diphosphooligosaccharide--protein glycotransferase
type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
EC number: dolichyl-diphosphooligosaccharide-protein glycotransferase
Source (natural)Organism: Chaetomium thermophilum (fungus) / Strain: DSM 1495 / CBS 144.50 / IMI 039719
Molecular weightTheoretical: 105.169227 KDa
SequenceString: MSSNGKVKAG QLWSKNKEEL TKILGELKTE LSQLRIQKIS SSGAKLNKIH DLRKSIARVL TVINAKQRAQ LRLFYKNKKY LPLDLRPKL TRALRRRLSK EDASRVLEKT KKRLTHFPQR KYAVKAATYS EGPALSIYHS HQREARCSLV QCCTFEPELR E SLLLLPVA ...String:
MSSNGKVKAG QLWSKNKEEL TKILGELKTE LSQLRIQKIS SSGAKLNKIH DLRKSIARVL TVINAKQRAQ LRLFYKNKKY LPLDLRPKL TRALRRRLSK EDASRVLEKT KKRLTHFPQR KYAVKAATYS EGPALSIYHS HQREARCSLV QCCTFEPELR E SLLLLPVA ASLKGPNFTR RERQHRNPSE ATMSAAEPLK LLASAGKGKS TRSVLRVAIL VLIAGAAVAS RLFSVIRFES II HEFDPWF NFRATKYLVA NGFYKFWDWF DDRTWHPLGR VTGGTLYPGL MVTSGVIYHL LRFLTVPVDI RNICVLLAPG FSG LTAIAA YLLTNEMTTS PSAGLLAAAF MGIAPGYISR SVAGSYDNEA IAIFLLVFTF FLWIKALKQG SMLWGALCAL FYGY MVASW GGYAFITCLL PLHSFVLICM GRYSTRLYVA YTTWYALGTL ASMQIPFVGF LPVKTSEHMP ALGIFGFLQL LAFLD YVRS TISSRQFQTF LWLFAGGIFG LGLLGLVIAT SAGLIAPWSG RFYSLWDTGY AKIHIPIIAS VSEHQPTAWP AFFFDL NML VWLFPVGVYL CFQQLGDEHV FIIVYALFGS YFAGVMVRLM LTLTPVVCVA AAIAFSSLLD TYLNLKTPNP GQAQATE DA GKKKSGLKAA SKPAVGVYAL WGKTMMISGL TIYLLLFVLH CTWVTSNAYS SPSVVLASRL PDGSQHIIDD YREAYQWL R QNTREDAKIM SWWDYGYQIG GMADRPTLVD NNTWNNTHIA TVGKAMASRE EVSYPIMRQH EVDYVLVVFG GLLGYSGDD INKFLWMVRI AEGIWPDEVS ERAFFTPRGE YRVDAEATDT MKNSLMYKMC YYNYNNLFPP GQAVDRMRGV RLPEVGPTLN TLEEAFTSE NWIIRIYKVK DLDNLGRDHA SAAAFERGHK KKKATKKRGP RVLRVE

UniProtKB: dolichyl-diphosphooligosaccharide--protein glycotransferase

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Macromolecule #28: 60S ribosomal protein L36

MacromoleculeName: 60S ribosomal protein L36 / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chaetomium thermophilum (fungus) / Strain: DSM 1495 / CBS 144.50 / IMI 039719
Molecular weightTheoretical: 12.784053 KDa
SequenceString:
MSEDATPKAP VERTGLIRGL NKGHKTTRRV LKERPSRRKG AQSKRTQFVR SLIREVAGLA PYERRVIELL RNGKDKRARK FSKKKLGTF GRAKRKCEEL QRIIAESRRA H

UniProtKB: 60S ribosomal protein L36

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Macromolecule #29: Ribosomal protein L37

MacromoleculeName: Ribosomal protein L37 / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chaetomium thermophilum (fungus) / Strain: DSM 1495 / CBS 144.50 / IMI 039719
Molecular weightTheoretical: 10.660455 KDa
SequenceString:
MTKGTSSFGK RHNKTHGLCR RCGRRSLHNQ KKVCASCGYP AAKTRKYNWS EKAKRRKVTG TGRMRYLSTV PRRFKNGFRT GVPKGARGP AVSTSS

UniProtKB: Ribosomal protein L37

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Macromolecule #30: Ribosomal RNA-processing protein 1

MacromoleculeName: Ribosomal RNA-processing protein 1 / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chaetomium thermophilum (fungus) / Strain: DSM 1495 / CBS 144.50 / IMI 039719
Molecular weightTheoretical: 32.128604 KDa
SequenceString: MTSQEKSMPF IKHLASSDRK VRTAALNSLH AFLSARQVAS ALTTLDVLKL WKGLFYALWM CDRAIPQQNL CNELADLIWQ LPRESVATW LRGFWATMAR EWTGIDVLRM EKFLLLVRRV LGASFKWMKK DGSLGKRTHD GQEKAVKTGA WDQSKVDEVL G LLAEWPFS ...String:
MTSQEKSMPF IKHLASSDRK VRTAALNSLH AFLSARQVAS ALTTLDVLKL WKGLFYALWM CDRAIPQQNL CNELADLIWQ LPRESVATW LRGFWATMAR EWTGIDVLRM EKFLLLVRRV LGASFKWMKK DGSLGKRTHD GQEKAVKTGA WDQSKVDEVL G LLAEWPFS LAEEVRITQS SEKGGEIVQK IPVGMRLHVL DIWVDEVERV GLLNEDEEEA RMIVQRISDM VDALEQTTKS PA VRTRSKD SLGDDRLPAN RRPNSSQDHD TKDMGDSDSW EGFDD

UniProtKB: Ribosomal RNA-processing protein 1

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Macromolecule #31: Brix domain-containing protein

MacromoleculeName: Brix domain-containing protein / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chaetomium thermophilum (fungus) / Strain: DSM 1495 / CBS 144.50 / IMI 039719
Molecular weightTheoretical: 49.471938 KDa
SequenceString: MAASKSKDLS RVSLARTIGN KLKRQQLYIQ AKKANSRAKH EERHRRRKEE AKNPELKQKR LEKNQPASID KKRIWDDLDD DSLGAVVDV AELKRRRLEV AEAAAAAEAD AAAREDQEVD DKNDENEKRD DDIDSMLGSD DEDEEDEGAK KEKEEEMQRQ R AQRQPSIA ...String:
MAASKSKDLS RVSLARTIGN KLKRQQLYIQ AKKANSRAKH EERHRRRKEE AKNPELKQKR LEKNQPASID KKRIWDDLDD DSLGAVVDV AELKRRRLEV AEAAAAAEAD AAAREDQEVD DKNDENEKRD DDIDSMLGSD DEDEEDEGAK KEKEEEMQRQ R AQRQPSIA PSTTSTNLDL TPDSLTSQFP NLFADEPPPV PKILVTTGIN GTIHKEAEEI ASIFPNATYI RRTAHRYAYK YS VREIAKF AKNRGYTALL VVQEDQKRPS RLVVVHLNGE GVPPGPTLTY TIRNYAPGKA IPGHGRATNH YPELLLNNFK TPL GLLAAK SMHILFPSKP ELAGRQVVTL HNQRDYIFFR RHRYVFREAR PTEKNVVGAD GKEMEGVKGI RAGLQEIGPR MTLK LRRVD KGVGRAGSEG PDAVKWEWKA KMEKKRTRFN L

UniProtKB: Brix domain-containing protein

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Macromolecule #32: Protein MAK16

MacromoleculeName: Protein MAK16 / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Chaetomium thermophilum (fungus) / Strain: DSM 1495 / CBS 144.50 / IMI 039719
Molecular weightTheoretical: 39.464891 KDa
SequenceString: MSDEIVWQII NQQFCAFKLK TTKGQNFCRN EYSVSGLCNR QSCPLANSRY ATVRQSPKGT IYLYIKTIER SHMPSKWWEK IKLSQNYQK ALEQIDQRLQ FFPKFLIHKC KQRLTRLVQV AIRMRRIAAE EARLGEKLVP KMAPKIKKRE EARERKALAA A KLERTIER ...String:
MSDEIVWQII NQQFCAFKLK TTKGQNFCRN EYSVSGLCNR QSCPLANSRY ATVRQSPKGT IYLYIKTIER SHMPSKWWEK IKLSQNYQK ALEQIDQRLQ FFPKFLIHKC KQRLTRLVQV AIRMRRIAAE EARLGEKLVP KMAPKIKKRE EARERKALAA A KLERTIER ELLDRLRQGA YGDQPLNCNP QIWKKVLNAL EAEGEGVRDK DHDKGIEADE EGLESAEESE VEYEREEENE ED NVVEYVS DFEESEDELG DLEDWLGSDD EEFDDEDDDD EDDDDEDDDE SEEDDEEELK KKKAGDKRKR GKAVKPKARK RKE LEIERE EADRAKLLAF

UniProtKB: Protein MAK16

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Macromolecule #33: ZINC ION

MacromoleculeName: ZINC ION / type: ligand / ID: 33 / Number of copies: 2 / Formula: ZN
Molecular weightTheoretical: 65.409 Da

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7.4
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELDBright-field microscopy / Nominal defocus max: 2.5 µm / Nominal defocus min: 0.8 µm
Image recordingFilm or detector model: GATAN K2 SUMMIT (4k x 4k) / Detector mode: COUNTING / Average electron dose: 44.0 e/Å2
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Startup modelType of model: EMDB MAP
EMDB ID:

3891

Initial angle assignmentType: OTHER / Details: Relion
Final angle assignmentType: OTHER / Details: Relion
Final reconstructionResolution.type: BY AUTHOR / Resolution: 3.0 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 83243
FSC plot (resolution estimation)

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