EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Molecular basis of differential receptor usage for naturally occurring CD55-binding and -nonbinding coxsackievirus B3 strains.
ジャーナル・号・ページ	Proc Natl Acad Sci U S A, Vol. 119, Issue 4, Year 2022
掲載日	2022年1月25日
著者	Qingling Wang / Qian Yang / Congcong Liu / Guoqing Wang / Hao Song / Guijun Shang / Ruchao Peng / Xiao Qu / Sheng Liu / Yingzi Cui / Peiyi Wang / Wenbo Xu / Xin Zhao / Jianxun Qi / Mengsu Yang / George F Gao /
PubMed 要旨	Receptor usage defines cell tropism and contributes to cell entry and infection. Coxsackievirus B (CVB) engages coxsackievirus and adenovirus receptor (CAR), and selectively utilizes the decay- ...Receptor usage defines cell tropism and contributes to cell entry and infection. Coxsackievirus B (CVB) engages coxsackievirus and adenovirus receptor (CAR), and selectively utilizes the decay-accelerating factor (DAF; CD55) to infect cells. However, the differential receptor usage mechanism for CVB remains elusive. This study identified VP3-234 residues (234Q/N/V/D/E) as critical population selection determinants during CVB3 virus evolution, contributing to diverse binding affinities to CD55. Cryoelectron microscopy (cryo-EM) structures of CD55-binding/nonbinding isolates and their complexes with CD55 or CAR were obtained under both neutral and acidic conditions, and the molecular mechanism of VP3-234 residues determining CD55 affinity/specificity for naturally occurring CVB3 strains was elucidated. Structural and biochemical studies in vitro revealed the dynamic entry process of CVB3 and the function of the uncoating receptor CAR with different pH preferences. This work provides detailed insight into the molecular mechanism of CVB infection and contributes to an in-depth understanding of enterovirus attachment receptor usage.
リンク	Proc Natl Acad Sci U S A / PubMed:35046043 / PubMed Central
手法	EM (単粒子)
解像度	2.2 - 3.68 Å
構造データ	32179 7vxh EMDB-32179, PDB-7vxh: Coxsackievirus B3 full particle at pH7.4 (VP3-234Q) 手法: EM (単粒子) / 解像度: 2.95 Å 32189 7vxz EMDB-32189, PDB-7vxz: Coxsackievirus B3 at pH7.4 (VP3-234Q) incubation with coxsackievirus and adenovirus receptor for 20min 手法: EM (単粒子) / 解像度: 3.19 Å 32190 7vy0 EMDB-32190, PDB-7vy0: Coxsackievirus B3 full particle at pH7.4 (VP3-234N) 手法: EM (単粒子) / 解像度: 2.7 Å 32194 7vy5 EMDB-32194, PDB-7vy5: Coxsackievirus B3 (VP3-234Q) incubation with CD55 at pH7.4 手法: EM (単粒子) / 解像度: 3.15 Å 32195 7vy6 EMDB-32195, PDB-7vy6: Coxsackievirus B3(VP3-234N) incubate with CD55 at pH7.4 手法: EM (単粒子) / 解像度: 3.02 Å 32207 7vyk EMDB-32207, PDB-7vyk: Coxsackievirus B3 at pH7.4 (VP3-234Q) incubation with coxsackievirus and adenovirus receptor for 10min 手法: EM (単粒子) / 解像度: 2.79 Å 32208 7vyl EMDB-32208, PDB-7vyl: Coxsackievirus B3 at pH5.5 (VP3-234Q) incubation with coxsackievirus and adenovirus receptor for 20min 手法: EM (単粒子) / 解像度: 2.79 Å 32209 7vym EMDB-32209, PDB-7vym: Coxsackievirus B3 at pH7.4 (VP3-234E) incubation with coxsackievirus and adenovirus receptor for 10min 手法: EM (単粒子) / 解像度: 3.68 Å 32250 7w14 EMDB-32250, PDB-7w14: Coxsackievirus B3 at pH7.4 (VP3-234E) incubation with coxsackievirus and adenovirus receptor for 20min 手法: EM (単粒子) / 解像度: 2.2 Å 32251 7w17 EMDB-32251, PDB-7w17: Coxsackievirus B3 full particle at pH7.4 (VP3-234E) 手法: EM (単粒子) / 解像度: 2.5 Å
化合物	ChemComp-PLM: PALMITIC ACID / パルミチン酸
由来	coxsackievirus b3 (コクサッキーウイルス) homo sapiens (ヒト)
キーワード	VIRUS / CVB3 / VP3-234Q / full particle / coxsackievirus and adenovirus receptor / 20min / VP3-234N / CD55 / VP3-234E