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-Structure paper
タイトル | Rhoptry secretion system structure and priming in Plasmodium falciparum revealed using in situ cryo-electron tomography. |
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ジャーナル・号・ページ | Nat Microbiol, Vol. 7, Issue 8, Page 1230-1238, Year 2022 |
掲載日 | 2022年7月11日 |
著者 | Matthew Martinez / William David Chen / Marta Mendonça Cova / Petra Molnár / Shrawan Kumar Mageswaran / Amandine Guérin / Audrey R Odom John / Maryse Lebrun / Yi-Wei Chang / |
PubMed 要旨 | Apicomplexan parasites secrete contents of the rhoptries, club-shaped organelles in the apical region, into host cells to permit their invasion and establishment of infection. The rhoptry secretory ...Apicomplexan parasites secrete contents of the rhoptries, club-shaped organelles in the apical region, into host cells to permit their invasion and establishment of infection. The rhoptry secretory apparatus (RSA), which is critical for rhoptry secretion, was recently discovered in Toxoplasma and Cryptosporidium. It is unknown whether a similar molecular machinery exists in the malaria parasite Plasmodium. In this study, we use in situ cryo-electron tomography to investigate the rhoptry secretion system in P. falciparum merozoites. We identify the presence of an RSA at the cell apex and a morphologically distinct apical vesicle docking the tips of the two rhoptries to the RSA. We also discover two additional rhoptry organizations that lack the apical vesicle. Using subtomogram averaging, we reveal different conformations of the RSA structure corresponding to different rhoptry organizations. Our results highlight previously unknown steps in the process of rhoptry secretion and indicate a regulatory role for the conserved apical vesicle in host invasion by apicomplexan parasites. |
リンク | Nat Microbiol / PubMed:35817892 / PubMed Central |
手法 | EM (サブトモグラム平均) / EM (トモグラフィー) |
解像度 | 43.0 - 50.0 Å |
構造データ | EMDB-26670: Rhoptry secretory apparatus of Plasmodium falciparum with an associated apical vesicle EMDB-26671: Rhoptry secretory apparatus of Plasmodium falciparum with an associated rhoptry tip from a non-fused rhoptry pair EMDB-26672: Rhoptry secretory apparatus of Plasmodium falciparum with an associated rhoptry tip from a fused rhoptry pair EMDB-26745: Plasmodium falciparum merozoite with two rhoptries and an associated apical vesicle EMDB-26746: Plasmodium falciparum merozoite with two rhoptries that lack an apical vesicle EMDB-26747: Plasmodium falciparum merozoite with a fused rhoptry pair |
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