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- EMDB-26670: Rhoptry secretory apparatus of Plasmodium falciparum with an asso... -
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Open data
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Basic information
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Title | Rhoptry secretory apparatus of Plasmodium falciparum with an associated apical vesicle | |||||||||
![]() | Rhoptry secretory apparatus of Plasmodium falciparum with an associated apical vesicle | |||||||||
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![]() | Exocytosis Membrane Parasite / CELL INVASION | |||||||||
Biological species | ![]() ![]() ![]() | |||||||||
Method | subtomogram averaging / ![]() | |||||||||
![]() | Martinez M / Chang Y-W | |||||||||
Funding support | ![]()
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![]() | ![]() Title: Rhoptry secretion system structure and priming in Plasmodium falciparum revealed using in situ cryo-electron tomography. Authors: Matthew Martinez / William David Chen / Marta Mendonça Cova / Petra Molnár / Shrawan Kumar Mageswaran / Amandine Guérin / Audrey R Odom John / Maryse Lebrun / Yi-Wei Chang / ![]() ![]() Abstract: Apicomplexan parasites secrete contents of the rhoptries, club-shaped organelles in the apical region, into host cells to permit their invasion and establishment of infection. The rhoptry secretory ...Apicomplexan parasites secrete contents of the rhoptries, club-shaped organelles in the apical region, into host cells to permit their invasion and establishment of infection. The rhoptry secretory apparatus (RSA), which is critical for rhoptry secretion, was recently discovered in Toxoplasma and Cryptosporidium. It is unknown whether a similar molecular machinery exists in the malaria parasite Plasmodium. In this study, we use in situ cryo-electron tomography to investigate the rhoptry secretion system in P. falciparum merozoites. We identify the presence of an RSA at the cell apex and a morphologically distinct apical vesicle docking the tips of the two rhoptries to the RSA. We also discover two additional rhoptry organizations that lack the apical vesicle. Using subtomogram averaging, we reveal different conformations of the RSA structure corresponding to different rhoptry organizations. Our results highlight previously unknown steps in the process of rhoptry secretion and indicate a regulatory role for the conserved apical vesicle in host invasion by apicomplexan parasites. #1: ![]() Title: In situ structure and priming mechanism of the rhoptry secretion system in Plasmodium revealed by cryo-electron tomography Authors: Martinez M / Chen WD / Cova MM / Molnar P / Mageswaran SK / Guerin A / Odom John AR / Lebrun M / Chang Y-W | |||||||||
History |
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Structure visualization
Supplemental images |
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Downloads & links
-EMDB archive
Map data | ![]() | 2.5 MB | ![]() | |
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Header (meta data) | ![]() ![]() | 18.9 KB 18.9 KB | Display Display | ![]() |
FSC (resolution estimation) | ![]() | 3.3 KB | Display | ![]() |
Images | ![]() | 49.4 KB | ||
Masks | ![]() | 2.8 MB | ![]() | |
Filedesc metadata | ![]() | 5 KB | ||
Others | ![]() ![]() | 1.3 MB 1.3 MB | ||
Archive directory | ![]() ![]() | HTTPS FTP |
-Related structure data
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Links
EMDB pages | ![]() ![]() |
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Map
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Annotation | Rhoptry secretory apparatus of Plasmodium falciparum with an associated apical vesicle | ||||||||||||||||||||
Voxel size | X=Y=Z: 10.6 Å | ||||||||||||||||||||
Density |
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Symmetry | Space group: 1 | ||||||||||||||||||||
Details | EMDB XML:
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-Supplemental data
-Mask #1
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Projections & Slices |
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Density Histograms |
-Half map: Rhoptry secretory apparatus of Plasmodium falciparum with an...
File | emd_26670_half_map_1.map | ||||||||||||
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Annotation | Rhoptry secretory apparatus of Plasmodium falciparum with an associated apical vesicle | ||||||||||||
Projections & Slices |
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Density Histograms |
-Half map: Rhoptry secretory apparatus of Plasmodium falciparum with an...
File | emd_26670_half_map_2.map | ||||||||||||
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Annotation | Rhoptry secretory apparatus of Plasmodium falciparum with an associated apical vesicle | ||||||||||||
Projections & Slices |
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Density Histograms |
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Sample components
-Entire : Plasmodium falciparum merozoite
Entire | Name: Plasmodium falciparum merozoite |
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Components |
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-Supramolecule #1: Plasmodium falciparum merozoite
Supramolecule | Name: Plasmodium falciparum merozoite / type: cell / ID: 1 / Parent: 0 / Macromolecule list: #1-#2 |
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-Supramolecule #2: Rhoptry secretory apparatus
Supramolecule | Name: Rhoptry secretory apparatus / type: complex / ID: 2 / Parent: 1 / Macromolecule list: #1 Details: 8-fold symmetric protein structure, anchored into the plasma membrane, which docks the associated apical vesicle to the plasma membrane |
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Source (natural) | Organism: ![]() ![]() ![]() |
-Supramolecule #3: Apical vesicle
Supramolecule | Name: Apical vesicle / type: organelle_or_cellular_component / ID: 3 / Parent: 1 / Macromolecule list: #2 Details: A small vesicle at the rhoptry tips that is docked at the rhoptry secretory apparatus. |
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Source (natural) | Organism: ![]() ![]() ![]() |
-Experimental details
-Structure determination
Method | ![]() |
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![]() | subtomogram averaging |
Aggregation state | cell |
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Sample preparation
Buffer | pH: 7.4 Details: RPMI medium modified with 27 mM NaHCO3, 11 mM glucose, 5 mM HEPES, 0.01 mM thymidine, 1 mM sodium pyruvate, 0.37 mM hypoxanthine, 10 ug/mL gentamicin, and 5 g/L Albumax |
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Grid | Model: Quantifoil R2/2 / Material: COPPER / Mesh: 200 / Support film - Material: CARBON / Support film - topology: HOLEY / Pretreatment - Type: GLOW DISCHARGE |
Vitrification | Cryogen name: ETHANE-PROPANE / Chamber humidity: 95 % / Chamber temperature: 297 K / Instrument: LEICA EM GP Details: Blot for 4 or 6 seconds from the front before plunging. |
Details | Mechanically isolated merozoites from a highly synchronous culture of Plasmodium falciparum schizonts |
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Electron microscopy
Microscope | FEI TITAN KRIOS |
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Electron beam | Acceleration voltage: 300 kV / Electron source: ![]() |
Electron optics | C2 aperture diameter: 100.0 µm / Illumination mode: FLOOD BEAM / Imaging mode: BRIGHT FIELD![]() |
Specialist optics | Phase plate: VOLTA PHASE PLATE / Energy filter - Name: GIF Bioquantum / Energy filter - Slit width: 20 eV |
Sample stage | Specimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN |
Image recording | Film or detector model: GATAN K3 (6k x 4k) / Number grids imaged: 9 / Average exposure time: 0.45 sec. / Average electron dose: 2.5 e/Å2 Details: Images were collected in dose-fractionation mode with 0.1 second exposure per frame, for a total of 0.4 or 0.5 seconds per tilt image |
Experimental equipment | ![]() Model: Titan Krios / Image courtesy: FEI Company |