EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structural basis for heparan sulfate co-polymerase action by the EXT1-2 complex.
ジャーナル・号・ページ	Nat Chem Biol, Vol. 19, Issue 5, Page 565-574, Year 2023
掲載日	2023年1月2日
著者	Hua Li / Digantkumar Chapla / Robert A Amos / Annapoorani Ramiah / Kelley W Moremen / Huilin Li /
PubMed 要旨	Heparan sulfate (HS) proteoglycans are extended (-GlcAβ1,4GlcNAcα1,4-) co-polymers containing decorations of sulfation and epimerization that are linked to cell surface and extracellular matrix ...Heparan sulfate (HS) proteoglycans are extended (-GlcAβ1,4GlcNAcα1,4-) co-polymers containing decorations of sulfation and epimerization that are linked to cell surface and extracellular matrix proteins. In mammals, HS repeat units are extended by an obligate heterocomplex of two exostosin family members, EXT1 and EXT2, where each protein monomer contains distinct GT47 (GT-B fold) and GT64 (GT-A fold) glycosyltransferase domains. In this study, we generated human EXT1-EXT2 (EXT1-2) as a functional heterocomplex and determined its structure in the presence of bound donor and acceptor substrates. Structural data and enzyme activity of catalytic site mutants demonstrate that only two of the four glycosyltransferase domains are major contributors to co-polymer syntheses: the EXT1 GT-B fold β1,4GlcA transferase domain and the EXT2 GT-A fold α1,4GlcNAc transferase domain. The two catalytic sites are over 90 Å apart, indicating that HS is synthesized by a dissociative process that involves a single catalytic site on each monomer.
リンク	Nat Chem Biol / PubMed:36593275 / PubMed Central
手法	EM (単粒子)
解像度	2.8 - 3.4 Å
構造データ	25035 7sch EMDB-25035: Cryo-EM 3D map of the human Exostosin-1 and Exostosin-2 heterodimer PDB-7sch: Cryo-EM structure of the human Exostosin-1 and Exostosin-2 heterodimer 手法: EM (単粒子) / 解像度: 3.1 Å 25036 7scj EMDB-25036: Cryo-EM 3D map of the human Exostosin-1 and Exostosin-2 heterodimer in complex with a 4-sugar oligosaccharide acceptor analog PDB-7scj: Cryo-EM structure of the human Exostosin-1 and Exostosin-2 heterodimer in complex with a 4-sugar oligosaccharide acceptor analog 手法: EM (単粒子) / 解像度: 3.4 Å 25037 7sck EMDB-25037: Cryo-EM 3D map of the human Exostosin-1 and Exostosin-2 heterodimer in complex with a 7-sugar oligosaccharide acceptor analog PDB-7sck: Cryo-EM structure of the human Exostosin-1 and Exostosin-2 heterodimer in complex with a 7-sugar oligosaccharide acceptor analog 手法: EM (単粒子) / 解像度: 2.8 Å 26701 7uqx EMDB-26701, PDB-7uqx: Cryo-EM structure of the human Exostosin-1 and Exostosin-2 heterodimer in complex with UDP-GlcNAc 手法: EM (単粒子) / 解像度: 3.3 Å 26702 7uqy EMDB-26702, PDB-7uqy: Cryo-EM structure of the human Exostosin-1 and Exostosin-2 heterodimer in complex with UDP-GlcA 手法: EM (単粒子) / 解像度: 3.0 Å
化合物	ChemComp-NAG: 2-acetamido-2-deoxy-beta-D-glucopyranose / N-アセチル-β-D-グルコサミン ChemComp-UDP: URIDINE-5'-DIPHOSPHATE / UDP / UDPYM ChemComp-MN*: Unknown entry
由来	homo sapiens (ヒト)
キーワード	TRANSFERASE / exostosin1 / exostosin2 / glycosyltransferase / heparan sulfate / acceptor