EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Architecture and assembly mechanism of native glycine receptors.
ジャーナル・号・ページ	Nature, Vol. 599, Issue 7885, Page 513-517, Year 2021
掲載日	2021年9月23日
著者	Hongtao Zhu / Eric Gouaux /
PubMed 要旨	Glycine receptors (GlyRs) are pentameric, 'Cys-loop' receptors that form chloride-permeable channels and mediate fast inhibitory signalling throughout the central nervous system. In the spinal cord ...Glycine receptors (GlyRs) are pentameric, 'Cys-loop' receptors that form chloride-permeable channels and mediate fast inhibitory signalling throughout the central nervous system. In the spinal cord and brainstem, GlyRs regulate locomotion and cause movement disorders when mutated. However, the stoichiometry of native GlyRs and the mechanism by which they are assembled remain unclear, despite extensive investigation. Here we report cryo-electron microscopy structures of native GlyRs from pig spinal cord and brainstem, revealing structural insights into heteromeric receptors and their predominant subunit stoichiometry of 4α:1β. Within the heteromeric pentamer, the β(+)-α(-) interface adopts a structure that is distinct from the α(+)-α(-) and α(+)-β(-) interfaces. Furthermore, the β-subunit contains a unique phenylalanine residue that resides within the pore and disrupts the canonical picrotoxin site. These results explain why inclusion of the β-subunit breaks receptor symmetry and alters ion channel pharmacology. We also find incomplete receptor complexes and, by elucidating their structures, reveal the architectures of partially assembled α-trimers and α-tetramers.
リンク	Nature / PubMed:34555840 / PubMed Central
手法	EM (単粒子)
解像度	2.7 - 12.0 Å
構造データ	23910 7mlu EMDB-23910, PDB-7mlu: Cryo-EM reveals partially and fully assembled native glycine receptors,homomeric pentamer 手法: EM (単粒子) / 解像度: 4.1 Å 23911 7mlv EMDB-23911, PDB-7mlv: Cryo-EM reveals partially and fully assembled native glycine receptors,homomeric tetramer 手法: EM (単粒子) / 解像度: 4.1 Å EMDB-23912: Cryo-EM reveals partially and fully assembled native glycine receptors, homomeric trimer 手法: EM (単粒子) / 解像度: 12.0 Å 23913 7mly EMDB-23913, PDB-7mly: Cryo-EM reveals partially and fully assembled native glycine receptors,heteromeric pentamer 手法: EM (単粒子) / 解像度: 2.7 Å
化合物	ChemComp-NAG: 2-acetamido-2-deoxy-beta-D-glucopyranose / N-アセチル-β-D-グルコサミン ChemComp-MAN: alpha-D-mannopyranose / α-D-マンノピラノ-ス ChemComp-BMA: beta-D-mannopyranose / β-D-マンノピラノ-ス ChemComp-HP6: HEPTANE / ヘプタン-1,1,1-トリイルラジカル ChemComp-DD9: nonane / ノナン ChemComp-LNK: PENTANE / ペンタン ChemComp-MYS: PENTADECANE / ペンタデカン ChemComp-D10: DECANE / デカン ChemComp-NBU: N-BUTANE / ブタン ChemComp-GLY: GLYCINE / グリシン ChemComp-D12: DODECANE / ドデカン ChemComp-OCT: N-OCTANE / オクタン ChemComp-HEX: HEXANE / ヘキサン ChemComp-UND: UNDECANE / ウンデカン
由来	sus scrofa (ブタ) rattus norvegicus (ドブネズミ) Pig (ブタ)
キーワード	MEMBRANE PROTEIN / SIGNALING PROTEIN / glycine receptor / ion channel / homomeric pentamer / homomeric tetramer / heteromeric pentamer