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-Structure paper
タイトル | Structural basis of DNA packaging by a ring-type ATPase from an archetypal viral system. |
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ジャーナル・号・ページ | Nucleic Acids Res, Vol. 50, Issue 15, Page 8719-8732, Year 2022 |
掲載日 | 2022年8月26日 |
![]() | Herman K H Fung / Shelley Grimes / Alexis Huet / Robert L Duda / Maria Chechik / Joseph Gault / Carol V Robinson / Roger W Hendrix / Paul J Jardine / James F Conway / Christoph G Baumann / Alfred A Antson / ![]() ![]() |
PubMed 要旨 | Many essential cellular processes rely on substrate rotation or translocation by a multi-subunit, ring-type NTPase. A large number of double-stranded DNA viruses, including tailed bacteriophages and ...Many essential cellular processes rely on substrate rotation or translocation by a multi-subunit, ring-type NTPase. A large number of double-stranded DNA viruses, including tailed bacteriophages and herpes viruses, use a homomeric ring ATPase to processively translocate viral genomic DNA into procapsids during assembly. Our current understanding of viral DNA packaging comes from three archetypal bacteriophage systems: cos, pac and phi29. Detailed mechanistic understanding exists for pac and phi29, but not for cos. Here, we reconstituted in vitro a cos packaging system based on bacteriophage HK97 and provided a detailed biochemical and structural description. We used a photobleaching-based, single-molecule assay to determine the stoichiometry of the DNA-translocating ATPase large terminase. Crystal structures of the large terminase and DNA-recruiting small terminase, a first for a biochemically defined cos system, reveal mechanistic similarities between cos and pac systems. At the same time, mutational and biochemical analyses indicate a new regulatory mechanism for ATPase multimerization and coordination in the HK97 system. This work therefore establishes a framework for studying the evolutionary relationships between ATP-dependent DNA translocation machineries in double-stranded DNA viruses. |
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手法 | EM (単粒子) / X線回折 |
解像度 | 1.4 - 22.0 Å |
構造データ | ![]() EMDB-22099: ![]() EMDB-22100: ![]() EMDB-22101: ![]() PDB-6z6d: ![]() PDB-6z6e: |
化合物 | ![]() ChemComp-BR: ![]() ChemComp-HOH: ![]() ChemComp-IOD: |
由来 |
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![]() | VIRAL PROTEIN / genome packaging / bacteriophage / ATPase / nuclease / DNA binding |