EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	LetB Structure Reveals a Tunnel for Lipid Transport across the Bacterial Envelope.
ジャーナル・号・ページ	Cell, Vol. 181, Issue 3, Page 653-664.e19, Year 2020
掲載日	2020年4月30日
著者	Georgia L Isom / Nicolas Coudray / Mark R MacRae / Collin T McManus / Damian C Ekiert / Gira Bhabha /
PubMed 要旨	Gram-negative bacteria are surrounded by an outer membrane composed of phospholipids and lipopolysaccharide, which acts as a barrier and contributes to antibiotic resistance. The systems that mediate ...Gram-negative bacteria are surrounded by an outer membrane composed of phospholipids and lipopolysaccharide, which acts as a barrier and contributes to antibiotic resistance. The systems that mediate phospholipid trafficking across the periplasm, such as MCE (Mammalian Cell Entry) transporters, have not been well characterized. Our ~3.5 Å cryo-EM structure of the E. coli MCE protein LetB reveals an ~0.6 megadalton complex that consists of seven stacked rings, with a central hydrophobic tunnel sufficiently long to span the periplasm. Lipids bind inside the tunnel, suggesting that it functions as a pathway for lipid transport. Cryo-EM structures in the open and closed states reveal a dynamic tunnel lining, with implications for gating or substrate translocation. Our results support a model in which LetB establishes a physical link between the two membranes and creates a hydrophobic pathway for the translocation of lipids across the periplasm.
リンク	Cell / PubMed:32359438 / PubMed Central
手法	EM (単粒子) / X線回折
解像度	2.15 - 3.78 Å
構造データ	20993 6v0c EMDB-20993: Lipophilic Envelope-spanning Tunnel B (LetB), Map 1 PDB-6v0c: Lipophilic Envelope-spanning Tunnel B (LetB), Model 1 手法: EM (単粒子) / 解像度: 3.46 Å 20994 6v0d EMDB-20994: Lipophilic Envelope-spanning Tunnel B (LetB), Map 2 PDB-6v0d: Lipophilic Envelope-spanning Tunnel B (LetB), Model 2 手法: EM (単粒子) / 解像度: 3.49 Å 20995 6v0e EMDB-20995: Lipophilic Envelope-spanning Tunnel B (LetB), Map 3 PDB-6v0e: Lipophilic Envelope-spanning Tunnel B (LetB), Model 3 手法: EM (単粒子) / 解像度: 3.06 Å 20996 6v0f EMDB-20996: Lipophilic Envelope-spanning Tunnel B (LetB), Map 4 PDB-6v0f: Lipophilic Envelope-spanning Tunnel B (LetB), Model 4 手法: EM (単粒子) / 解像度: 2.96 Å 20997 6v0g EMDB-20997: Lipophilic Envelope-spanning Tunnel B (LetB), Map 5 PDB-6v0g: Lipophilic Envelope-spanning Tunnel B (LetB), Model 5 手法: EM (単粒子) / 解像度: 3.03 Å 20998 6v0h EMDB-20998: Lipophilic Envelope-spanning Tunnel B (LetB), Map 6 PDB-6v0h: Lipophilic Envelope-spanning Tunnel B (LetB), Model 6 手法: EM (単粒子) / 解像度: 3.6 Å 20999 6v0i EMDB-20999: Lipophilic Envelope-spanning Tunnel B (LetB), Map 7 PDB-6v0i: Lipophilic Envelope-spanning Tunnel B (LetB), Model 7 手法: EM (単粒子) / 解像度: 3.43 Å 21000 6v0j EMDB-21000: Lipophilic Envelope-spanning Tunnel B (LetB), Map 8 PDB-6v0j: Lipophilic Envelope-spanning Tunnel B (LetB), Model 8 手法: EM (単粒子) / 解像度: 3.78 Å PDB-6vci: Lipophilic envelope-spanning tunnel protein (LetB), domains MCE2-MCE3 手法: X-RAY DIFFRACTION / 解像度: 2.15 Å
化合物	ChemComp-HOH: WATER
由来	Escherichia coli K-12 (大腸菌) escherichia coli (strain k12) (大腸菌)
キーワード	LIPID TRANSPORT / bacterial cell envelope / MCE / YebT / conformational dynamics / LetB