EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structures of the AMPA receptor in complex with its auxiliary subunit cornichon.
ジャーナル・号・ページ	Science, Vol. 366, Issue 6470, Page 1259-1263, Year 2019
掲載日	2019年12月6日
著者	Terunaga Nakagawa /
PubMed 要旨	In the brain, AMPA-type glutamate receptors (AMPARs) form complexes with their auxiliary subunits and mediate the majority of fast excitatory neurotransmission. Signals transduced by these complexes ...In the brain, AMPA-type glutamate receptors (AMPARs) form complexes with their auxiliary subunits and mediate the majority of fast excitatory neurotransmission. Signals transduced by these complexes are critical for synaptic plasticity, learning, and memory. The two major categories of AMPAR auxiliary subunits are transmembrane AMPAR regulatory proteins (TARPs) and cornichon homologs (CNIHs); these subunits share little homology and play distinct roles in controlling ion channel gating and trafficking of AMPAR. Here, I report high-resolution cryo-electron microscopy structures of AMPAR in complex with CNIH3. Contrary to its predicted membrane topology, CNIH3 lacks an extracellular domain and instead contains four membrane-spanning helices. The protein-protein interaction interface that dictates channel modulation and the lipids surrounding the complex are revealed. These structures provide insights into the molecular mechanism for ion channel modulation and assembly of AMPAR/CNIH3 complexes.
リンク	Science / PubMed:31806817
手法	EM (単粒子)
解像度	2.97 - 4.4 Å
構造データ	20330 6peq EMDB-20330, PDB-6peq: GluA2 in complex with its auxiliary subunit CNIH3 - map LBD-TMD-C3 - with antagonist ZK200775 -without NTD 手法: EM (単粒子) / 解像度: 2.97 Å EMDB-20332: GluA2 full length in complex with its auxiliary subunit CNIH3 in PS - with antagonist ZK200775 手法: EM (単粒子) / 解像度: 4.4 Å 20654 6u5s EMDB-20654, PDB-6u5s: NTD of GluA2 in complex with CNIH3 - with antagonist ZK200775 - in pseudo-symmetric global conformation 手法: EM (単粒子) / 解像度: 3.07 Å 20666 6u6i EMDB-20666, PDB-6u6i: NTD of GluA2 in complex with CNIH3 - with antagonist ZK200775 - in asymmetric global conformation 手法: EM (単粒子) / 解像度: 3.12 Å EMDB-20717: GluA2 in complex with complex full-length in AS, bound to antagonist ZK200775 手法: EM (単粒子) / 解像度: 4.4 Å 20727 6ucb EMDB-20727, PDB-6ucb: GluA2 in complex with its auxiliary subunit CNIH3 - with antagonist ZK200775, LBD, TMD, CNIH3, and lipids 手法: EM (単粒子) / 解像度: 3.28 Å EMDB-20732: GluA2 in complex with its auxiliary subunit CNIH3 in AS map I - (LBD-TMD-C3(AS) I) - with antagonist ZK200775, without NTD 手法: EM (単粒子) / 解像度: 3.5 Å 20733 6ud4 EMDB-20733, PDB-6ud4: GluA2 in complex with its auxiliary subunit CNIH3 in AS map II - (LBD-TMD-C3(AS) II)- with antagonist ZK200775, without NTD 手法: EM (単粒子) / 解像度: 3.3 Å 20734 6ud8 EMDB-20734, PDB-6ud8: GluA2 in complex with its auxiliary subunit CNIH3 - with antagonist ZK200775 手法: EM (単粒子) / 解像度: 3.2 Å
化合物	ChemComp-ZK1: {[7-morpholin-4-yl-2,3-dioxo-6-(trifluoromethyl)-3,4-dihydroquinoxalin-1(2H)-yl]methyl}phosphonic acid / ZK-200775 / アンタゴニスト, 薬剤YM ChemComp-OLC: (2R)-2,3-dihydroxypropyl (9Z)-octadec-9-enoate / L-グリセロ-ル3-オレア-ト ChemComp-PAM: PALMITOLEIC ACID / パルミトレイン酸 ChemComp-CLR: CHOLESTEROL / コレステロ-ル ChemComp-NAG*: 2-acetamido-2-deoxy-beta-D-glucopyranose / N-アセチル-β-D-グルコサミン
由来	rattus norvegicus (ドブネズミ) mus musculus (ハツカネズミ)
キーワード	TRANSPORT PROTEIN / ionotropic glutamate receptor / AMPA receptor / cornichon / auxiliary subunit / ion channel / ligand gated ion channel / synaptic transmission / excitatory synaptic transmission / neurotransmitter receptor / stargazin / TARP / ZK200775 / lipid / MPQX