EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Antibody Lineages with Vaccine-Induced Antigen-Binding Hotspots Develop Broad HIV Neutralization.
ジャーナル・号・ページ	Cell, Vol. 178, Issue 3, Page 567-584.e19, Year 2019
掲載日	2019年7月25日
著者	Rui Kong / Hongying Duan / Zizhang Sheng / Kai Xu / Priyamvada Acharya / Xuejun Chen / Cheng Cheng / Adam S Dingens / Jason Gorman / Mallika Sastry / Chen-Hsiang Shen / Baoshan Zhang / Tongqing Zhou / Gwo-Yu Chuang / Cara W Chao / Ying Gu / Alexander J Jafari / Mark K Louder / Sijy O'Dell / Ariana P Rowshan / Elise G Viox / Yiran Wang / Chang W Choi / Martin M Corcoran / Angela R Corrigan / Venkata P Dandey / Edward T Eng / Hui Geng / Kathryn E Foulds / Yicheng Guo / Young D Kwon / Bob Lin / Kevin Liu / Rosemarie D Mason / Martha C Nason / Tiffany Y Ohr / Li Ou / Reda Rawi / Edward K Sarfo / Arne Schön / John P Todd / Shuishu Wang / Hui Wei / Winston Wu / / James C Mullikin / Robert T Bailer / Nicole A Doria-Rose / Gunilla B Karlsson Hedestam / Diana G Scorpio / Julie Overbaugh / Jesse D Bloom / Bridget Carragher / Clinton S Potter / Lawrence Shapiro / Peter D Kwong / John R Mascola /
PubMed 要旨	The vaccine-mediated elicitation of antibodies (Abs) capable of neutralizing diverse HIV-1 strains has been a long-standing goal. To understand how broadly neutralizing antibodies (bNAbs) can be ...The vaccine-mediated elicitation of antibodies (Abs) capable of neutralizing diverse HIV-1 strains has been a long-standing goal. To understand how broadly neutralizing antibodies (bNAbs) can be elicited, we identified, characterized, and tracked five neutralizing Ab lineages targeting the HIV-1-fusion peptide (FP) in vaccinated macaques over time. Genetic and structural analyses revealed two of these lineages to belong to a reproducible class capable of neutralizing up to 59% of 208 diverse viral strains. B cell analysis indicated each of the five lineages to have been initiated and expanded by FP-carrier priming, with envelope (Env)-trimer boosts inducing cross-reactive neutralization. These Abs had binding-energy hotspots focused on FP, whereas several FP-directed Abs induced by immunization with Env trimer-only were less FP-focused and less broadly neutralizing. Priming with a conserved subregion, such as FP, can thus induce Abs with binding-energy hotspots coincident with the target subregion and capable of broad neutralization.
リンク	Cell / PubMed:31348886 / PubMed Central
手法	EM (単粒子) / X線回折
解像度	1.99 - 4.3 Å
構造データ	20189 6osy EMDB-20189, PDB-6osy: Cryo-EM structure of vaccine-elicited antibody 0PV-a.01 in complex with HIV-1 Env BG505 DS-SOSIP and antibodies VRC03 and PGT122 手法: EM (単粒子) / 解像度: 4.3 Å 20191 6ot1 EMDB-20191, PDB-6ot1: Cryo-EM structure of vaccine-elicited antibody 0PV-b.01 in complex with HIV-1 Env BG505 DS-SOSIP and antibodies VRC03 and PGT122 手法: EM (単粒子) / 解像度: 3.5 Å 8977 6mpg EMDB-8977, PDB-6mpg: Cryo-EM structure at 3.2 A resolution of HIV-1 fusion peptide-directed antibody, A12V163-b.01, elicited by vaccination of Rhesus macaques, in complex with stabilized HIV-1 Env BG505 DS-SOSIP, which was also bound to antibodies VRC03 and PGT122 手法: EM (単粒子) / 解像度: 3.18 Å 9189 6mph EMDB-9189, PDB-6mph: Cryo-EM structure at 3.8 A resolution of HIV-1 fusion peptide-directed antibody, DF1W-a.01, elicited by vaccination of Rhesus macaques, in complex with stabilized HIV-1 Env BG505 DS-SOSIP, which was also bound to antibodies VRC03 and PGT122 手法: EM (単粒子) / 解像度: 3.8 Å 9319 6n1v EMDB-9319, PDB-6n1v: Cryo-EM structure at 4.0 A resolution of vaccine-elicited antibody A12V163-a.01 in complex with HIV-1 Env BG505 DS-SOSIP, and antibodies VRC03 and PGT122 手法: EM (単粒子) / 解像度: 4.0 Å 9320 6n1w EMDB-9320, PDB-6n1w: Cryo-EM structure at 4.2 A resolution of vaccine-elicited antibody DFPH-a.15 in complex with HIV-1 Env BG505 DS-SOSIP, and antibodies VRC03 and PGT122 手法: EM (単粒子) / 解像度: 4.2 Å 9359 6nf2 EMDB-9359, PDB-6nf2: Cryo-EM structure of vaccine-elicited antibody 0PV-c.01 in complex with HIV-1 Env BG505 DS-SOSIP and antibodies VRC03 and PGT122 手法: EM (単粒子) / 解像度: 3.7 Å PDB-6mqc: Vaccine-elicited NHP FP-targeting neutralizing antibody 0PV-c.01 in complex with FP (residue 512-519) 手法: X-RAY DIFFRACTION / 解像度: 1.99 Å PDB-6mqe: Vaccine-elicited NHP FP-targeting HIV neutralizing antibody DFPH-a.15 in complex with HIV fusion peptide (residue 512-519) 手法: X-RAY DIFFRACTION / 解像度: 2.459 Å PDB-6mqm: Vaccine-elicited NHP FP-targeting neutralizing antibody DF1W-a.01 in complex with HIV fusion peptide (residue 512-519) 手法: X-RAY DIFFRACTION / 解像度: 3.484 Å PDB-6mqr: Vaccine-elicited NHP FP-targeting neutralizing antibody 0PV-a.01 in complex with FP (residue 512-519) 手法: X-RAY DIFFRACTION / 解像度: 2.45 Å PDB-6mqs: Vaccine-elicited NHP FP-targeting HIV neutralizing antibody A12V163-a.01 in complex with HIV fusion peptide (residue 512-519) 手法: X-RAY DIFFRACTION / 解像度: 2.997 Å PDB-6n16: Vaccine-elicited NHP FP-targeting neutralizing antibody 0PV-b.01 in complex with HIV fusion peptide (residue 512-519) 手法: X-RAY DIFFRACTION / 解像度: 2.302 Å
化合物	ChemComp-NAG: 2-acetamido-2-deoxy-beta-D-glucopyranose / N-アセチル-β-D-グルコサミン ChemComp-HOH: WATER ChemComp-SO4: SULFATE ION / 硫酸ジアニオン ChemComp-CA: Unknown entry / カルシウムジカチオン
由来	human immunodeficiency virus 1 (ヒト免疫不全ウイルス) homo sapiens (ヒト) macaca mulatta (アカゲザル)
キーワード	VIRAL PROTEIN / Complex / Fusion peptide / Neutralizing antibody / HIV-1 Envelope / IMMUNE SYSTEM / HIV / neutralizing / NHP / FP / vaccine / HIV-1 Env complex / fusion peptide-directed / HIV-1 / SOSIP