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-Structure paper
タイトル | Unconventional stabilization of the human T-cell leukemia virus type 1 immature Gag lattice. |
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ジャーナル・号・ページ | bioRxiv, Year 2023 |
掲載日 | 2023年7月24日 |
著者 | Martin Obr / Mathias Percipalle / Darya Chernikova / Huixin Yang / Andreas Thader / Gergely Pinke / Dario Porley / Louis M Mansky / Robert A Dick / Florian Km Schur / |
PubMed 要旨 | Human T-cell leukemia virus type 1 (HTLV-1) has an atypical immature particle morphology compared to other retroviruses. This indicates that these particles are formed in a way that is unique. Here ...Human T-cell leukemia virus type 1 (HTLV-1) has an atypical immature particle morphology compared to other retroviruses. This indicates that these particles are formed in a way that is unique. Here we report the results of cryo-electron tomography (cryo-ET) studies of HTLV-1 virus-like particles (VLPs) assembled , as well as derived from cells. This work shows that HTLV-1 employs an unconventional mechanism of Gag-Gag interactions to form the immature viral lattice. Analysis of high-resolution structural information from immature CA tubular arrays reveals that the primary stabilizing component in HTLV-1 is CA-NTD. Mutagenesis and biophysical analysis support this observation. This distinguishes HTLV-1 from other retroviruses, in which the stabilization is provided primarily by the CA-CTD. These results are the first to provide structural details of the quaternary arrangement of Gag for an immature deltaretrovirus, and this helps explain why HTLV-1 particles are morphologically distinct. |
リンク | bioRxiv / PubMed:37546793 / PubMed Central |
手法 | EM (サブトモグラム平均) / EM (トモグラフィー) |
解像度 | 3.4 - 6.9 Å |
構造データ | EMDB-17929, PDB-8pu6: EMDB-17930, PDB-8pu7: EMDB-17931, PDB-8pu8: EMDB-17932, PDB-8pu9: EMDB-17933, PDB-8pua: EMDB-17934, PDB-8pub: EMDB-17935, PDB-8puc: EMDB-17936, PDB-8pud: EMDB-17937, PDB-8pue: EMDB-17938: Cryo-electron tomogram of HTLV-1 MA126CANC tubes EMDB-17939: Cryo-electron tomogram of HTLV-1 MA126CANC tubes EMDB-17940, PDB-8puf: EMDB-17941: Cryo-electron tomogram containing HTLV-1 Gag-based VLPs EMDB-17942, PDB-8pug: EMDB-17943, PDB-8puh: |
由来 |
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キーワード | VIRAL PROTEIN / Retrovirus / HTLV / immature capsid / CA / CA-NTD |