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-Structure paper
タイトル | Structure of the actively translating plant 80S ribosome at 2.2 Å resolution. |
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ジャーナル・号・ページ | Nat Plants, Vol. 9, Issue 6, Page 987-981000, Year 2023 |
掲載日 | 2023年5月8日 |
著者 | Julia Smirnova / Justus Loerke / Gunnar Kleinau / Andrea Schmidt / Jörg Bürger / Etienne H Meyer / Thorsten Mielke / Patrick Scheerer / Ralph Bock / Christian M T Spahn / Reimo Zoschke / |
PubMed 要旨 | In plant cells, translation occurs in three compartments: the cytosol, the plastids and the mitochondria. While the structures of the (prokaryotic-type) ribosomes in plastids and mitochondria are ...In plant cells, translation occurs in three compartments: the cytosol, the plastids and the mitochondria. While the structures of the (prokaryotic-type) ribosomes in plastids and mitochondria are well characterized, high-resolution structures of the eukaryotic 80S ribosomes in the cytosol have been lacking. Here the structure of translating tobacco (Nicotiana tabacum) 80S ribosomes was solved by cryo-electron microscopy with a global resolution of 2.2 Å. The ribosome structure includes two tRNAs, decoded mRNA and the nascent peptide chain, thus providing insights into the molecular underpinnings of the cytosolic translation process in plants. The map displays conserved and plant-specific rRNA modifications and the positions of numerous ionic cofactors, and it uncovers the role of monovalent ions in the decoding centre. The model of the plant 80S ribosome enables broad phylogenetic comparisons that reveal commonalities and differences in the ribosomes of plants and those of other eukaryotes, thus putting our knowledge about eukaryotic translation on a firmer footing. |
リンク | Nat Plants / PubMed:37156858 / PubMed Central |
手法 | EM (単粒子) |
解像度 | 2.14 - 2.38 Å |
構造データ | EMDB-15674, PDB-8auv: EMDB-15773, PDB-8azw: EMDB-15806, PDB-8b2l: |
化合物 | ChemComp-MG: ChemComp-K: ChemComp-SPD: ChemComp-SPM: ChemComp-ZN: ChemComp-HOH: |
由来 |
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キーワード | RIBOSOME / Plant / 80S / Modifications / rRNA Modifications |