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- EMDB-15773: Cryo-EM structure of the plant 60S subunit -

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Basic information

Entry
Database: EMDB / ID: EMD-15773
TitleCryo-EM structure of the plant 60S subunit
Map dataThe large subunit of the tobacco ribosome from cytosol
Sample
  • Complex: 40S subunit of the cytosolic 80S ribosome from tobacco
    • RNA: x 1 types
    • Protein or peptide: x 34 types
  • RNA: x 2 types
  • Protein or peptide: x 9 types
  • Ligand: x 6 types
KeywordsPlant / 80S / rRNA Modifications / ribosome
Function / homology
Function and homology information


protein-RNA complex assembly / maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / maturation of LSU-rRNA / ribosomal large subunit biogenesis / ribosome biogenesis / 5S rRNA binding / large ribosomal subunit rRNA binding / cytosolic large ribosomal subunit / cytoplasmic translation / rRNA binding ...protein-RNA complex assembly / maturation of LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / maturation of LSU-rRNA / ribosomal large subunit biogenesis / ribosome biogenesis / 5S rRNA binding / large ribosomal subunit rRNA binding / cytosolic large ribosomal subunit / cytoplasmic translation / rRNA binding / negative regulation of translation / ribosome / structural constituent of ribosome / ribonucleoprotein complex / translation / mRNA binding / RNA binding / metal ion binding
Similarity search - Function
Ribosomal protein L6, N-terminal / Ribosomal protein L6, N-terminal domain / Ribosomal protein L28e / Ribosomal protein L23 / Ribosomal L28e/Mak16 / Ribosomal L28e protein family / Ribosomal protein L41 / Ribosomal protein L41 / metallochaperone-like domain / TRASH domain ...Ribosomal protein L6, N-terminal / Ribosomal protein L6, N-terminal domain / Ribosomal protein L28e / Ribosomal protein L23 / Ribosomal L28e/Mak16 / Ribosomal L28e protein family / Ribosomal protein L41 / Ribosomal protein L41 / metallochaperone-like domain / TRASH domain / Ribosomal protein L29e / Ribosomal L29e protein family / Ribosomal protein L13e, conserved site / Ribosomal protein L13e signature. / Ribosomal protein L22e / Ribosomal protein L22e superfamily / Ribosomal L22e protein family / Ribosomal protein L38e / Ribosomal protein L38e superfamily / Ribosomal L38e protein family / Ribosomal protein L27e, conserved site / Ribosomal protein L27e signature. / Ribosomal protein L44e signature. / Ribosomal protein L10e, conserved site / Ribosomal protein L10e signature. / Ribosomal protein L10e / Ribosomal protein L13e / Ribosomal protein L13e / Ribosomal protein L19, eukaryotic / 60S ribosomal protein L18a/ L20, eukaryotes / Ribosomal protein L24e, conserved site / Ribosomal protein L24e signature. / Ribosomal protein L44e / Ribosomal protein L19/L19e conserved site / Ribosomal protein L44 / Ribosomal protein L19e signature. / Ribosomal protein L34e, conserved site / Ribosomal protein L34e signature. / Ribosomal protein L5 eukaryotic, C-terminal / Ribosomal L18 C-terminal region / 50S ribosomal protein L18Ae/60S ribosomal protein L20 and L18a / Ribosomal protein L30e signature 1. / Ribosomal protein 50S-L18Ae/60S-L20/60S-L18A / Ribosomal proteins 50S-L18Ae/60S-L20/60S-L18A / Eukaryotic Ribosomal Protein L27, KOW domain / Ribosomal protein L23/L25, N-terminal / Ribosomal protein L23, N-terminal domain / Ribosomal protein L30e signature 2. / Ribosomal protein L30e, conserved site / Ribosomal protein 60S L18 and 50S L18e / Ribosomal protein L27e / Ribosomal protein L27e superfamily / Ribosomal L27e protein family / Ribosomal protein L36e signature. / Ribosomal protein L39e, conserved site / Ribosomal protein L39e signature. / Ribosomal protein L34Ae / Ribosomal protein L34e / 60S ribosomal protein L19 / Ribosomal protein L35Ae, conserved site / Ribosomal protein L35Ae signature. / Ribosomal protein L30/YlxQ / 60S ribosomal protein L35 / Ribosomal Protein L6, KOW domain / Ribosomal protein L13, eukaryotic/archaeal / Ribosomal protein L7A/L8 / Ribosomal protein L6e / 60S ribosomal protein L6E / Ribosomal protein L18e / 60S ribosomal protein L4, C-terminal domain / 60S ribosomal protein L4 C-terminal domain / Ribosomal protein L37ae / Ribosomal protein L7, eukaryotic / Ribosomal protein L31e, conserved site / Ribosomal L37ae protein family / Ribosomal protein L31e signature. / Ribosomal_L19e / Ribosomal protein L30, N-terminal / Ribosomal L30 N-terminal domain / Ribosomal protein L19/L19e / Ribosomal protein L19/L19e, domain 1 / Ribosomal protein L19/L19e superfamily / Ribosomal protein L19e / Ribosomal protein L36e / Ribosomal protein L36e domain superfamily / Ribosomal protein L36e / Ribosomal protein L39e / Ribosomal protein L14e domain / Ribosomal protein L39e domain superfamily / Ribosomal L39 protein / Ribosomal protein L14 / Ribosomal protein L5 eukaryotic/L18 archaeal / Ribosomal large subunit proteins 60S L5, and 50S L18 / Ribosomal protein L35A / Ribosomal protein L35Ae / Ribosomal protein L35A superfamily / Ribosomal protein L32e, conserved site / Ribosomal protein L32e signature. / Ribosomal protein L14 / Ribosomal protein L6, conserved site-2
Similarity search - Domain/homology
60S ribosomal protein L35-like / 60S ribosomal protein L30-like / Ribosomal protein L19 / 60S ribosomal protein L4-like / 60S ribosomal protein L37a / 60S ribosomal protein L11-1 / 60S ribosomal protein L21-1-like / 60S ribosomal protein L5-like / 60S ribosomal protein L23 / 60S ribosomal protein L35a-3 ...60S ribosomal protein L35-like / 60S ribosomal protein L30-like / Ribosomal protein L19 / 60S ribosomal protein L4-like / 60S ribosomal protein L37a / 60S ribosomal protein L11-1 / 60S ribosomal protein L21-1-like / 60S ribosomal protein L5-like / 60S ribosomal protein L23 / 60S ribosomal protein L35a-3 / 60S ribosomal protein L8-like / 60S ribosomal protein L31 / 60S ribosomal protein L29 / 60S ribosomal protein L39-3 / 60S ribosomal protein L10-like / 60S ribosomal protein L32-1 / 60S ribosomal protein L3 / 60S ribosomal protein L7-4-like / 60S ribosomal protein L14-1 / 60S ribosomal protein L27 / 60S ribosomal protein L44 / 60S ribosomal protein L6-like isoform X1 / 60S ribosomal protein L27a-3-like / 60S ribosomal protein L24-like / 60S ribosomal protein L22-2-like / 60S ribosomal protein L9-1-like / 60S ribosomal protein L38 / 60S ribosomal protein L17-1-like / 60S ribosomal protein L18a / 60S ribosomal protein L13 / 60S ribosomal protein L28-1-like / 60S ribosomal protein L36 / Ribosomal protein L15 / 60S ribosomal protein L7a / 60S ribosomal protein L18-2-like / 60S ribosomal protein L13a-4 / Ribosomal protein L37 / 60S ribosomal protein L26-1-like / Large ribosomal subunit protein eL34 / Small ribosomal subunit protein eS32 / Large ribosomal subunit protein uL23
Similarity search - Component
Biological speciesNicotiana tabacum (common tobacco) / common tobacco (common tobacco)
Methodsingle particle reconstruction / cryo EM / Resolution: 2.14 Å
AuthorsSmirnova J / Loerke J / Kleinau G / Schmidt A / Buerger J / Meyer EH / Mielke T / Scheerer P / Bock R / Spahn CMT / Zoschke R
Funding support Germany, 1 items
OrganizationGrant numberCountry
German Research Foundation (DFG)416210002 Germany
CitationJournal: Nat Plants / Year: 2023
Title: Structure of the actively translating plant 80S ribosome at 2.2 Å resolution.
Authors: Julia Smirnova / Justus Loerke / Gunnar Kleinau / Andrea Schmidt / Jörg Bürger / Etienne H Meyer / Thorsten Mielke / Patrick Scheerer / Ralph Bock / Christian M T Spahn / Reimo Zoschke /
Abstract: In plant cells, translation occurs in three compartments: the cytosol, the plastids and the mitochondria. While the structures of the (prokaryotic-type) ribosomes in plastids and mitochondria are ...In plant cells, translation occurs in three compartments: the cytosol, the plastids and the mitochondria. While the structures of the (prokaryotic-type) ribosomes in plastids and mitochondria are well characterized, high-resolution structures of the eukaryotic 80S ribosomes in the cytosol have been lacking. Here the structure of translating tobacco (Nicotiana tabacum) 80S ribosomes was solved by cryo-electron microscopy with a global resolution of 2.2 Å. The ribosome structure includes two tRNAs, decoded mRNA and the nascent peptide chain, thus providing insights into the molecular underpinnings of the cytosolic translation process in plants. The map displays conserved and plant-specific rRNA modifications and the positions of numerous ionic cofactors, and it uncovers the role of monovalent ions in the decoding centre. The model of the plant 80S ribosome enables broad phylogenetic comparisons that reveal commonalities and differences in the ribosomes of plants and those of other eukaryotes, thus putting our knowledge about eukaryotic translation on a firmer footing.
History
DepositionSep 6, 2022-
Header (metadata) releaseJun 7, 2023-
Map releaseJun 7, 2023-
UpdateJun 28, 2023-
Current statusJun 28, 2023Processing site: PDBe / Status: Released

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Structure visualization

Supplemental images

emd_15773.png

Downloads & links

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Map

FileDownload / File: emd_15773.map.gz / Format: CCP4 / Size: 347.6 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationThe large subunit of the tobacco ribosome from cytosol
Projections & slices

Image control

Size
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Others
AxesZ (Sec.)Y (Row.)X (Col.)
0.85 Å/pix.
x 450 pix.
= 381.6 Å		0.85 Å/pix.
x 450 pix.
= 381.6 Å		0.85 Å/pix.
x 450 pix.
= 381.6 Å

Surface

Projections

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Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 0.848 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 1.05
Minimum - Maximum-7.826657 - 15.518784
Average (Standard dev.)0.020157967 (±0.3137763)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions450450450
Spacing450450450
CellA=B=C: 381.6 Å
α=β=γ: 90.0 °

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Supplemental data

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Mask #1

Fileemd_15773_msk_1.map
Projections & Slices
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Projections

Slices (1/2)
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Histogram

Histogram (log scale)

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Half map: Half-map A of the tobacco ribosome after local refinement...

Fileemd_15773_half_map_1.map
AnnotationHalf-map_A of the tobacco ribosome after local refinement of the large subunit (60S)
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: Half-map B of the tobacco ribosome after local refinement...

Fileemd_15773_half_map_2.map
AnnotationHalf-map_B of the tobacco ribosome after local refinement of the large subunit (60S)
Projections & Slices
AxesZYX

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Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : 40S subunit of the cytosolic 80S ribosome from tobacco

EntireName: 40S subunit of the cytosolic 80S ribosome from tobacco
Components
  • Complex: 40S subunit of the cytosolic 80S ribosome from tobacco
    • RNA: 5S rRNA
    • Protein or peptide: eL13 (60S ribosomal protein L13)
    • Protein or peptide: eL14 (60S ribosomal protein L14)
    • Protein or peptide: eL15 (60S ribosomal protein L15)
    • Protein or peptide: eL18 (60S ribosomal protein L18)
    • Protein or peptide: eL19 (60S ribosomal protein L19)
    • Protein or peptide: eL20 (60S ribosomal protein L18a)
    • Protein or peptide: eL21 (60S ribosomal protein L21)
    • Protein or peptide: eL22 (60S ribosomal protein L22)
    • Protein or peptide: eL24 (60S ribosomal protein L24)
    • Protein or peptide: eL27 (60S ribosomal protein L27)
    • Protein or peptide: eL28 (60S ribosomal protein L28)
    • Protein or peptide: eL29 (60S ribosomal protein L29)
    • Protein or peptide: eL30 (60S ribosomal protein L30)
    • Protein or peptide: eL31 (60S ribosomal protein L31)
    • Protein or peptide: eL32 (60S ribosomal protein L32)
    • Protein or peptide: eL33 (60S ribosomal protein L35a)
    • Protein or peptide: eL34 (60S ribosomal protein L34)
    • Protein or peptide: eL36 (60S ribosomal protein L36)
    • Protein or peptide: eL37 (60S ribosomal protein L37)
    • Protein or peptide: eL38 (60S ribosomal protein L38)
    • Protein or peptide: eL39 (60S ribosomal protein L39)
    • Protein or peptide: eL40 (60S ribosomal protein L40)
    • Protein or peptide: eL41 (60S ribosomal protein L41)
    • Protein or peptide: eL42 (60S ribosomal protein L42)
    • Protein or peptide: eL43 (60S ribosomal protein L37a)
    • Protein or peptide: eL6 (60S ribosomal protein L6)
    • Protein or peptide: eL8 (60S ribosomal protein L7a)
    • Protein or peptide: uL13 (60S ribosomal protein L13a)
    • Protein or peptide: uL14 (60S ribosomal protein L23)
    • Protein or peptide: uL15 (60S ribosomal protein L27a)
    • Protein or peptide: uL16 (60S ribosomal protein L10)
    • Protein or peptide: uL18 (60S ribosomal protein L5)
    • Protein or peptide: uL22 (60S ribosomal protein L17)
    • Protein or peptide: uL23 (60S ribosomal protein L23a)
  • RNA: 25S rRNA
  • RNA: 5.8S rRNA
  • Protein or peptide: uL24 (60S ribosomal protein L26)
  • Protein or peptide: uL29 (60S ribosomal protein L35)
  • Protein or peptide: uL2 (60S ribosomal protein L8)
  • Protein or peptide: uL30 (60S ribosomal protein L7)
  • Protein or peptide: uL3 (60S ribosomal protein L3)
  • Protein or peptide: uL4 (60S ribosomal protein L4)
  • Protein or peptide: uL5 (60S ribosomal protein L11)
  • Protein or peptide: uL6 (60S ribosomal protein L9)
  • Protein or peptide: nascent chain
  • Ligand: MAGNESIUM ION
  • Ligand: POTASSIUM ION
  • Ligand: ZINC ION
  • Ligand: SPERMIDINE
  • Ligand: SPERMINE
  • Ligand: water

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Supramolecule #1: 40S subunit of the cytosolic 80S ribosome from tobacco

SupramoleculeName: 40S subunit of the cytosolic 80S ribosome from tobacco
type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#35
Source (natural)Organism: Nicotiana tabacum (common tobacco)

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Macromolecule #1: 5S rRNA

MacromoleculeName: 5S rRNA / type: rna / ID: 1 / Number of copies: 1
Source (natural)Organism: common tobacco (common tobacco)
Molecular weightTheoretical: 38.328777 KDa
SequenceString:
GGAUGCGAUC AUACCAGCAC UAACGCACCG GAUCCCAUCA GAACUCCGAA GUUAAGCGUG CUUGGGCGAG AGUAGUACUA GGAUGGGUG ACCCCCUGGG AAGUCCUCGU GUUGCAUCCC

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Macromolecule #45: 25S rRNA

MacromoleculeName: 25S rRNA / type: rna / ID: 45 / Number of copies: 1
Source (natural)Organism: common tobacco (common tobacco)
Molecular weightTheoretical: 1.098268 MDa
SequenceString: GCGACCCCAG GUCAGGCGGG ACUACCCGCU GAG(PSU)UUAAG(OMC) A(PSU)A(OMU)CAA(OMU)AA GCGGAGGAAA AGAAACUUA CAAGGAUUCC CCUAGUAACG GCGAGCGAAC CGGGAACAGC CCAGCCUUAG AAUCGGGCGG CUCCGCCGUU C GAA(OMU)UGUA ...String:
GCGACCCCAG GUCAGGCGGG ACUACCCGCU GAG(PSU)UUAAG(OMC) A(PSU)A(OMU)CAA(OMU)AA GCGGAGGAAA AGAAACUUA CAAGGAUUCC CCUAGUAACG GCGAGCGAAC CGGGAACAGC CCAGCCUUAG AAUCGGGCGG CUCCGCCGUU C GAA(OMU)UGUA G(PSU)CUGGAGAA GCGUCCUCAG CGGCGGACCG GGCCCAAGUC CCCUGGAAGG GGGCGCCAGA GAGG GUGAG AGCCCCGUUG (PSU)GCCCGGACC CUGUCGCACC ACGAGGCGCU GUCUACGAGU CGGGUUGUU(PSU) GGGAAUG CA GCCCAAAUC(OMG) GGCGGUGAAU UCCG(PSU)CCAAG GCUAAAUACG GGCGAGAGAC CGAUAGCGAA CAAGUACCGC GAGGGAAAG AUG(A2M)AAAGGA CUUUGAAAAG AGAGUCAAAG AGU(OMG)CUUGAA AUUGUCGGGA GGGAAGCGGA UGG GGGCCG GCGAUGCGCC CCGGUCGGAU GUGGAACGG(PSU) GACGAGCCGG UCCGCCGAUC GACUCGGGGC GUGGACCAGC GUGGA(PSU)UGG GGGGGCGGCC AAAGCCCGGG CUUUCGAUAC GCCCGUGGAA CGCCGUCUCC UUGAUUGUGG UAGGCAG CG CGCGCCUCCG GCGUGCUUCG GCAUCUGCGC GCUCCGGACG CUGGCCUGUG GGCUCCCCAU UCGACCCGUC UUG(1MA) AAC(A2M)C GGACCAAGGA GU(OMC)(OMU)GACAUG UG(PSU)GCGAGUC AACGGGCGAG UAAACCCGUA AGGCG (PSU)AAG GAAGCUGAUU GGUGGGAUCC CCCUGAGGGG UGCACCGCCG ACCGACCUUG AUCUUCUGUG AAGGG(PSU) (OMU)CG AGUGUGAGCA UACC(OMU)GUCGG GACCC(OMG)A(A2M)AG AUGGUGA(A2M)C(PSU) AUGCCUGAGC GGG GCGAAG CCAGAGGAAA CUCUGGUGGA GGCCCGCAGC GAUACUG(A2M)CG UGCAAA(PSU)CGU UCG(PSU)CUGACU UG GGUAUAG (OMG)GGCGAAAGA CUAAUCGAAC CGUCUAGU(A2M)G CUGGUUCCCU CCGAAGUU(PSU)C CC(PSU)CAGG A(PSU) AGCUGGAGCU CGCGUGCGAG UUCUA(PSU)CGGG UAAAGCCAA(PSU) GAUUAGAGGC AUCGGGGGCG CAACGC CCU CGACCUAUUC (PSU)CAAACUU(PSU)A AA(OMU)AGGUAGG ACGGUGCGGC UGCUUUGUUG AGCCGCACCA CGGAA UCAA GAGCUCCAAG UGGGCCAU(PSU)(PSU) (PSU)UGGUAAGC(A2M) GAACUGGCGA UGCGGGAUGA ACCGGAAGCC GGGUUACGG UGCCAAACUG CGCGCUAACC UAGAUCCCAC AAAGGGUGUU GGUCGAUUAA GACAGCAGGA CGGUGGUCAU G GAAGUCGA AAUCCGCUAA GGAGUGUGUA ACAACUCACC UGCCGAAUCA ACUAGCCCCG AAAAUGGAUG GCGCUUAAGC GC GCGACCU ACACCCGGCC GUCGGGGCAA GUGCCAGGCC CCGAUG(A2M)GUA GGAGGGCGCG GCGGUCGCUG CAAAACCUU GGGCGCGAGC CUGGGCGGAG CGGCCGUCGG UGCAGAU(OMC)UU GGUGGUAGU(A2M) (OMG)CAAAUAUUC AAA(PSU) GAGAA (OMC)U(PSU)UGAAGGC CGAAGAGGGG AAAGGUUCCA UGUGAACGGC ACUUGCACAU GGGUUAG(OMU)CG AUC CUAAGG GUCGGGGGAA CCCCGACAGA UAGCGCGUUU CGCGCGUACU CCGAAAGGGA AUCGGGUUAA AAUUCCUGAA CCGG GACGU GGCGGUUGAC GGCAACGUUA GGAAGUCCGG AGACGUCGGC GGGGGCCUCG GGAAGAG(PSU)UA UCUUUUCUGU U UAACAGCC UGCCCACCCU GGAAACGACU CAGUCGGAGG UAGGGUCCAG CGGCUGGAAG AGCACCGCAC GUCGCGUGGU GU CCGGUGC GCCCCCGGCG GCCCUUGAAA AUCCGGAGGA CCGAGUGCCG UCCACGCCCG GUCGUACUCA UAA(OMC)CG (OMC)AU CA(OMG)GUCU(OMC)CA AGGUGAACAG CCUCUGGUCG AUGGAACAA(OMU) GUAGGCAAGG GAAGUCGGCA A AAUGGAUC CGUAACUUCG GGAAAAGGAU UGGCUCUGAG GGCUGGGCAC GGGGGUCCCA GUCCCGAACC CGUCGGCUGU CG GUGGACU GCUCGAGCUG CUCCCGCGGC GAGAGCGGGU CGCCGCGUGC CGGCCGGGGG ACGGACUGGG AACGGUUCCU UCG GGGGCC UUCCCCGGGC GUCGAACAGC CAACUCAGAA CUGGUACGGA CAAG(OMG)(OMG)G(A2M)AU CCGAC(PSU)G (PSU)U UAAUUAAAAC AAAGCAUUGC GAUGGUCCCU GCGGAUGUUU ACGCAAUGUG AUU(PSU)CUGCC(OMC) AGUGCU CUG AAUG(PSU)CAAAG UGA(A2M)GAAAUU CAACCAAGC(OMG) CGGGUAAACG GCGGGAG(PSU)A(A2M) C(PSU)A (PSU)GAC(PSU)C (PSU)CUUAAGGUA GC(5MC)AA(A2M)UGCC UC(OMG)UCAU(OMC)UA AUUAGUGACG CGCAUG AA(PSU) GGA(PSU)UA(A2M)CGA G(A2M)UUCCCACU GU(OMC)CCUGUCU AC(OMU)AUCCAGC GAAACCACAG (OMC)CAAGGGAA CGGGCUUGGC AGAAUCA(OMG)CG G(OMG)GAAAGAAG ACCCU(OMG)(OMU)UGA GC(PSU)UGAC (OMU)C UAGUCCGAC(PSU) UUGUGAAAUG ACUUGAGAGG UGUAGUAUAA GUGGGAGCCG AAAGGCGAAA GUGAAAUACC ACUACUUUU AACGUUAUUU UACUUAUUCC GUGAAUCGGA AGCGGGGCAC UGCCCCUCUU UUUGGACCCA AGGCUUGCUU G CAGGCCGA UCCGGGCGGA AGACAUUGUC AGGUGGGGAG UUUGGCUG(OMG)G GCGGCACAUC UGUUAAAAG(A2M) UAAC GCAGG (UY1)(OMG)UCCUAAGA UGAGCUCAAC GAGAACAGAA AU(OMC)UCGUGUG GAACAGAAGG GUAAAAGCUC GUU UGAU(OMU)C UGAUUUCCAG (OMU)ACGAA(OMU)ACG AACCG(PSU)GAAA GCGUGGCCUA ACGAUCCUUU AGACCUUC G GAAUUCGAAG CUA(OMG)A(OMG)GUGU CAG(A2M)AAAGUU ACCACAG(OMG)GA UAACUGGC(PSU)U GUGGCAGC (OMC) AAGCGUUCAU AGCGACG(PSU)UG CUUUUUGA(PSU)C CUU(5MC)GAUGUC GG(OMC)(PSU)CU(OMU)CCU A UCAUUG(PSU)G AAGCAGAAUU CACCA(A2M)GUGU U(OMG)GAU(OMU)(OMG)(PSU)UC ACCCACCAAU AGGGAACG (PSU) G(A2M)G(OMC)UGGG(UR3)U (PSU)AGA(OMC)CGUCG UGAGACAGGU (PSU)AGUUUUACC CUACUGAUGA C AGUGUCGC AAUAGUAAUU CAACCUAGUA CGAGAGGAAC CGUUGAUUCG CACAAUUGGU CAUCGCGCUU GGUUGAAAAG CC AGUGGCG CGAAGCUACC GUGCGCUGGA UUAUGAC(PSU)GA ACGCCUCUAA GUCAGAAUCC GGGCUAGAAG CGACGCAUG CGCCCGCCGU CCGCUUGCCG ACCCGCAGUA GGGGCCUUUG GCCCCCAAGG GCACGUGUCG UUGGCUAAGU CAUCGUGGCG GAAGAGCCG CGGUGGCCGC CUUGAAGUAC AAUUUCCAUC GAGCGGCGGG UAGAA(OMU)CCUU UGCAGACGAC (OMU)UA AAUACG CGACGGGGUA UUGUAAGUGG CAGAGUGGCC UUGCUGCCAC GAUCCACUGA GAUUCAGCCC UUUGUCGCUC CGAU UCGU

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Macromolecule #46: 5.8S rRNA

MacromoleculeName: 5.8S rRNA / type: rna / ID: 46 / Number of copies: 1
Source (natural)Organism: common tobacco (common tobacco)
Molecular weightTheoretical: 52.563258 KDa
SequenceString:
AAACGACUCU CGGCAACGGA (PSU)AUCUCGGCU CUCGCAUCGA UGAAG(A2M)ACGU AGCGAAAUGC GAUACUUGGU GU GAAU(PSU)(OMG)C AGAAUUCCGU GAACCA(PSU)CGA GUCUUUGAAC GCAAGUUGCG CCCGAAGCCA UUAGGCCGAG G GCACGUCU GCCUGGGCGU CACGC

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Macromolecule #2: eL13 (60S ribosomal protein L13)

MacromoleculeName: eL13 (60S ribosomal protein L13) / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: common tobacco (common tobacco)
Molecular weightTheoretical: 23.595523 KDa
SequenceString: MKHNNVIPNG HFKKHWQNYV RTWFNQPARK TRRRAARQQK AVKIFPRPTA GSLRPIVHGQ TLKYNMKVRA GRGFSLEELK AAGIPKKLA PTIGIAVDHR RRNRSLEGLQ TNVQRLKTYK AKLVIFPRRA KKVKAGDSSA EELATATQVQ GSYMPITREQ P AVDLVKVT ...String:
MKHNNVIPNG HFKKHWQNYV RTWFNQPARK TRRRAARQQK AVKIFPRPTA GSLRPIVHGQ TLKYNMKVRA GRGFSLEELK AAGIPKKLA PTIGIAVDHR RRNRSLEGLQ TNVQRLKTYK AKLVIFPRRA KKVKAGDSSA EELATATQVQ GSYMPITREQ P AVDLVKVT DEMKSFNAYG KLRIERTNAR HIGARLKRAA EAEKEEKK

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Macromolecule #3: eL14 (60S ribosomal protein L14)

MacromoleculeName: eL14 (60S ribosomal protein L14) / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: common tobacco (common tobacco)
Molecular weightTheoretical: 15.443432 KDa
SequenceString:
MPFKRFVEIG RVALVNYGKD YGKLVVIVDV IDQNRALIDA PDMVRSQINF KRLSLTDIKI DIKRIPKKKT LVAAMEAADV KNKWESSSW GRKLIVQKRR ASLNDFDRFK LMLAKIKRAG VVRQELAKLK KETVA

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Macromolecule #4: eL15 (60S ribosomal protein L15)

MacromoleculeName: eL15 (60S ribosomal protein L15) / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: common tobacco (common tobacco)
Molecular weightTheoretical: 24.228266 KDa
SequenceString: MGAYTYVSEL WRKKQSDVMR FLQRVRCWEY RQLPSIVRVT RPTRPDKARR LGYKAKQGYV VYRVRVKRGG RKRPVPKGIV YGKPTNQGV TQLKFQRSKR SVAEERAGRK LGGLKVLNSY WINEDSTYKY YEVILVDAAH AAIRNDPRIN WICNPVHKHR E LRGLTSAG ...String:
MGAYTYVSEL WRKKQSDVMR FLQRVRCWEY RQLPSIVRVT RPTRPDKARR LGYKAKQGYV VYRVRVKRGG RKRPVPKGIV YGKPTNQGV TQLKFQRSKR SVAEERAGRK LGGLKVLNSY WINEDSTYKY YEVILVDAAH AAIRNDPRIN WICNPVHKHR E LRGLTSAG KKYRGLRGKG HLYHKNRPSR RGTWKRNQTL SLRRYR

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Macromolecule #5: eL18 (60S ribosomal protein L18)

MacromoleculeName: eL18 (60S ribosomal protein L18) / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: common tobacco (common tobacco)
Molecular weightTheoretical: 20.905824 KDa
SequenceString:
MGIDLKAGGK SKKTKRTAPK SDDVYLKLLV KLYRFLVRRT GSKFNAVILK RLFMSKINKP PLSLSRLISY AKGKEDKVVV IVGTITDDV RAYEVPALKV CALRFTKTAR ARIEKAGGEC LTFDQLALRA PLGQNTLLLR GPKNAREAVK HFGPAPGVPH S HTKPFVRS KGRKFERARG RRNSRGYKA

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Macromolecule #6: eL19 (60S ribosomal protein L19)

MacromoleculeName: eL19 (60S ribosomal protein L19) / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: common tobacco (common tobacco)
Molecular weightTheoretical: 25.05574 KDa
SequenceString: MVSLKLQKRL AASVLKCGRG KVWLDPNEGN EISMANSRQN IRKLVKDGFI IRKPTKIHSR SRARRMKEAK RKGRHSGYGK RKGTREARL PTKVLWMRRM RVLRRLLRKY RESKKIDRHM YHDMYMKVKG NVFKNKRVLM ENIHKTKAEK AREKTLSDQF E ARRAKNKA ...String:
MVSLKLQKRL AASVLKCGRG KVWLDPNEGN EISMANSRQN IRKLVKDGFI IRKPTKIHSR SRARRMKEAK RKGRHSGYGK RKGTREARL PTKVLWMRRM RVLRRLLRKY RESKKIDRHM YHDMYMKVKG NVFKNKRVLM ENIHKTKAEK AREKTLSDQF E ARRAKNKA SRERKFARRE ERLAQGPVER PMQPAAPAAS ATPAQTAQGG SKKSKK

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Macromolecule #7: eL20 (60S ribosomal protein L18a)

MacromoleculeName: eL20 (60S ribosomal protein L18a) / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: common tobacco (common tobacco)
Molecular weightTheoretical: 21.513348 KDa
SequenceString:
MVTYKFHQYQ VVGRALPTEN DEHPKIYRMK LWATNEVRAK SKFWYYLRKL KKVKKSNGQM LAINEIFEKN PTKIKNYGIW LRYQSRTGY HNMYKEYRDT TLNGAVEQMY TEMASRHRVR HHCIQIIKTA TIPAKLCKRE STKQFHDSKI KFPLVFKKVR P PSRKLKTT YKATRPNLFM

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Macromolecule #8: eL21 (60S ribosomal protein L21)

MacromoleculeName: eL21 (60S ribosomal protein L21) / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: common tobacco (common tobacco)
Molecular weightTheoretical: 18.716885 KDa
SequenceString:
MPAGHGLRSR TRDSFSRAFR KKGTIPLSTY LRIFKIGDYV DIKVNGAIHK GMPHKFYHGR TGRVWNVTKR AVGVEVNKQV RNKILRKRI HVRIEHVQLS RCTEEVKERM KKNDQLKAEA KARGEVISTK RQPVGPKPGF MVEGATLETV TPIPYDVVND L KGGY

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Macromolecule #9: eL22 (60S ribosomal protein L22)

MacromoleculeName: eL22 (60S ribosomal protein L22) / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: common tobacco (common tobacco)
Molecular weightTheoretical: 14.277366 KDa
SequenceString:
MSKGSSGGGA MKGGKKKGAT FVIDCSKPVE DKIMEIASLE KFLQERIKVG GKAGALGDSV TVTRDKTKIT VTSDTTFSKR YLKYLTKKY LKKNNVRDWL RVISSNKDRN VYELRYFNIA ENEAEEED

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Macromolecule #10: eL24 (60S ribosomal protein L24)

MacromoleculeName: eL24 (60S ribosomal protein L24) / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: common tobacco (common tobacco)
Molecular weightTheoretical: 18.606016 KDa
SequenceString:
MVLKTELCRF SGGKIYPGRG IRFIRSDSQV FLFLNSKCKR YFHNRLKPSK LTWTAMYRKQ HKKDIAQEAV KKRRRATKKP YSRSIVGAT LEVIQKKRTE RPEVRDAARE AALREIKERI KKTKDEKKAK KAEVQAKSQK AGGKGNMPKG AGGKGPKLGG G GGKR

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Macromolecule #11: eL27 (60S ribosomal protein L27)

MacromoleculeName: eL27 (60S ribosomal protein L27) / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: common tobacco (common tobacco)
Molecular weightTheoretical: 15.722707 KDa
SequenceString:
MVKFLKPNKA VIILQGKYAG RKAVIIRAFD EGTRDRPYGH CLVAGISKYP KKVIRKDSAK KQAKKSRVKT FIKLVNYNHI MPTRYTLDV DLKDVVNVDV LQSRDKKVTA AKEAKARLEE RFKTGKNRWF FTKLRF

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Macromolecule #12: eL28 (60S ribosomal protein L28)

MacromoleculeName: eL28 (60S ribosomal protein L28) / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: common tobacco (common tobacco)
Molecular weightTheoretical: 15.954613 KDa
SequenceString:
MTTVPGQLVW EIVKKNNSFL VKEFGNGTAG VVFSKEPNNL YNLHSYKHSG LANKKTVTIQ AEGKDKSVLL ATSKTKKQNK PSTLLNKSV MKKEFRRMSK AVTNQVADNY YRPDLKKAAL ARLSAVNRSL KVAKSGVKKK NRQA

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Macromolecule #13: eL29 (60S ribosomal protein L29)

MacromoleculeName: eL29 (60S ribosomal protein L29) / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: common tobacco (common tobacco)
Molecular weightTheoretical: 7.132044 KDa
SequenceString:
MAKSKNHTAH NQSYKAHRNG IKKPRKHRHS STKGMDPKFL RNQRYARKHN NNKSVGSADE E

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Macromolecule #14: eL30 (60S ribosomal protein L30)

MacromoleculeName: eL30 (60S ribosomal protein L30) / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: common tobacco (common tobacco)
Molecular weightTheoretical: 12.420518 KDa
SequenceString:
MATTGKKTKK THESINNRLA LVMKSGKYSL GYKTVLKTLR SSKGKLILIS NNCPPLRKSE IEYYAMLAKV GVHHFNGNNV DLGTACGKY FRVSCLSIID PGDSDIIKSL PGDH

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Macromolecule #15: eL31 (60S ribosomal protein L31)

MacromoleculeName: eL31 (60S ribosomal protein L31) / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: common tobacco (common tobacco)
Molecular weightTheoretical: 13.894171 KDa
SequenceString:
MSDKTKGRKE EVVTREYTIN LHKRLHSCTF KKKAPTAIKE IRKFAQKAMG TKDVRVDVKL NKQIWSRGIR SVPRRVRVRI ARKRNDDED AKEELYSLVT VAEIPAEGLK GLGTKIIEDE D

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Macromolecule #16: eL32 (60S ribosomal protein L32)

MacromoleculeName: eL32 (60S ribosomal protein L32) / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: common tobacco (common tobacco)
Molecular weightTheoretical: 15.665528 KDa
SequenceString:
MAVPLLTKKV VKKRVKRFIR PQSDRRITVK ESWRRPKGID SRVRRKFKGC VLMPNIGYGS DKKTRHYLPN GFKKFVVHNA SELEVLMMH NRTYCAEIAH NVSTRKRKEI VERAAQLDVV ITNKLARLRS QEDE

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Macromolecule #17: eL33 (60S ribosomal protein L35a)

MacromoleculeName: eL33 (60S ribosomal protein L35a) / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: common tobacco (common tobacco)
Molecular weightTheoretical: 12.959179 KDa
SequenceString:
MVKGRQGERV RLYVRGTVLG YKRSKSNQYP STSLIQIEGV NTKEEVDWYL GKRMAYIYKA KTKKNNSHYR CIWGKVCRPH GNSGVVRAK FKSNLPPKSM GSKVRVFMYP SNI

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Macromolecule #18: eL34 (60S ribosomal protein L34)

MacromoleculeName: eL34 (60S ribosomal protein L34) / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: common tobacco (common tobacco)
Molecular weightTheoretical: 13.788358 KDa
SequenceString:
MVQRLTYRKR HSYATKSNQH RVVKTPGGKL IYQSTKKRAS GPKCPVTGKR IQGIPHLRPT EYKRSRLSRN RRTVNRAYGG VLSGSAVRE RIIRAFLVEE QKIVKKVLKI QKAKEKLAAK S

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Macromolecule #19: eL36 (60S ribosomal protein L36)

MacromoleculeName: eL36 (60S ribosomal protein L36) / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: common tobacco (common tobacco)
Molecular weightTheoretical: 12.361817 KDa
SequenceString:
MAPKQPNTGL SVGLNKGHVV TKKELAPRPS DRKGKTSKRV HFVRSLIREV AGFAPYEKRI TELLKVGKDK RALKVAKRKL GTHKRAKKK REEMSNALRK MRAAGGGEKK K

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Macromolecule #20: eL37 (60S ribosomal protein L37)

MacromoleculeName: eL37 (60S ribosomal protein L37) / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: common tobacco (common tobacco)
Molecular weightTheoretical: 10.879783 KDa
SequenceString:
MGKGTGSFGK RRNKTHTLCV RCGRRSFHIQ KSRCSACAYP AARLRKYNWS VKALRRKTTG TGRMRYLRNV PRRFKTNFRE GTEATPRKK GAAVAS

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Macromolecule #21: eL38 (60S ribosomal protein L38)

MacromoleculeName: eL38 (60S ribosomal protein L38) / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: common tobacco (common tobacco)
Molecular weightTheoretical: 8.101704 KDa
SequenceString:
MPKQIHEIKD FLLTARRKDA RTVKIKKNKD MVKFKVRCSK YLYTLCVSDF EKADKLKQSL PPGLSVQDL

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Macromolecule #22: eL39 (60S ribosomal protein L39)

MacromoleculeName: eL39 (60S ribosomal protein L39) / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: common tobacco (common tobacco)
Molecular weightTheoretical: 6.503839 KDa
SequenceString:
MPSHKTFMIK KKLAKKQRQN RPIPYWIRMR TDNTIRYNAK RRHWRRTKLG F

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Macromolecule #23: eL40 (60S ribosomal protein L40)

MacromoleculeName: eL40 (60S ribosomal protein L40) / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: common tobacco (common tobacco)
Molecular weightTheoretical: 14.717386 KDa
SequenceString:
MQIFVKTLTG KTITLEVESS DTIDNVKAKI QDKEGIPPDQ QRLIFAGKQL EDGRTLADYN IQKESTLHLV LRLRGGIIEP SLMALARKY NQEKMICRKC YARLHPRAVN CRKKKCGHSN QLRPKKKIK

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Macromolecule #24: eL41 (60S ribosomal protein L41)

MacromoleculeName: eL41 (60S ribosomal protein L41) / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: common tobacco (common tobacco)
Molecular weightTheoretical: 3.445437 KDa
SequenceString:
MRAKWKKKRM RRLKRKRRKM RQRSK

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Macromolecule #25: eL42 (60S ribosomal protein L42)

MacromoleculeName: eL42 (60S ribosomal protein L42) / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: common tobacco (common tobacco)
Molecular weightTheoretical: 12.037346 KDa
SequenceString:
MVNVPKTKKT YCKSKECKKH TLHKVTQYKK GKDSLAAQGK RRYDRKQSGY GGQTKPVFHK KAKTTKKIVL RLQCQGCKHV SQHPIKRCK HFEIGGDKKG KGTSLF

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Macromolecule #26: eL43 (60S ribosomal protein L37a)

MacromoleculeName: eL43 (60S ribosomal protein L37a) / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: common tobacco (common tobacco)
Molecular weightTheoretical: 10.280108 KDa
SequenceString:
MTKRTKKAGI VGKYGTRYGA SLRKQIKKME VSQHSKYFCE FCGKYAVKRK AVGIWGCKDC GKVKAGGAYT LNTASAVTVR STIRRLREQ TES

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Macromolecule #27: eL6 (60S ribosomal protein L6)

MacromoleculeName: eL6 (60S ribosomal protein L6) / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: common tobacco (common tobacco)
Molecular weightTheoretical: 25.536387 KDa
SequenceString: MAAKKSPRNP ELIRGVGKLS RSKMYHKKGL WAIKKKNGGS FPVHKKAAAV APPAVKPPKF YPADDVAKPL VNKHKPKPTK LRASITPGT VLIILAGRFK GKRVVFLKQL KSGLLLVSGP FKLNGVPLRR VNQAYVIGTS TKVDISGVNV EKIDDKYFAK Q AEKKQKKG ...String:
MAAKKSPRNP ELIRGVGKLS RSKMYHKKGL WAIKKKNGGS FPVHKKAAAV APPAVKPPKF YPADDVAKPL VNKHKPKPTK LRASITPGT VLIILAGRFK GKRVVFLKQL KSGLLLVSGP FKLNGVPLRR VNQAYVIGTS TKVDISGVNV EKIDDKYFAK Q AEKKQKKG EGEFFEDKKE EKKNVLPQGK KDDQKAVDEA LIKAVECVPE LKAYLSARFS LKSCMKPHEL VF

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Macromolecule #28: eL8 (60S ribosomal protein L7a)

MacromoleculeName: eL8 (60S ribosomal protein L7a) / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: common tobacco (common tobacco)
Molecular weightTheoretical: 29.432219 KDa
SequenceString: MAPKKGVAVA AKKKPVQAKV VNPLFEKRPK QFGIGGALPP KKDLTRFVRW PQVVRIQRKR RILKQRLKVP PSLNQFSKTL DKNLATNLF KMLLKYRPED KAAKKERLLK RAQAEAEGKT PETKKPIIVK YGLKHITYLI EQNKAQLVVI AHDVDPIELV V WLPALCRK ...String:
MAPKKGVAVA AKKKPVQAKV VNPLFEKRPK QFGIGGALPP KKDLTRFVRW PQVVRIQRKR RILKQRLKVP PSLNQFSKTL DKNLATNLF KMLLKYRPED KAAKKERLLK RAQAEAEGKT PETKKPIIVK YGLKHITYLI EQNKAQLVVI AHDVDPIELV V WLPALCRK MEIPYCIVKG KARLGSIVHK KTASALCLTT VKNEDKMEFS RILEAIKANF NDKYEENRKK WGGGVMGSKS QA RTKAKER VLAKEAAQRM N

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Macromolecule #29: uL13 (60S ribosomal protein L13a)

MacromoleculeName: uL13 (60S ribosomal protein L13a) / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: common tobacco (common tobacco)
Molecular weightTheoretical: 23.525217 KDa
SequenceString: MVSGSGISAR RIVVDARHHM LGRLSSILAK ELLNGQKVVV VRCEEICLSG GLVRQKMKYL RFLRKRMNTK PSHGPIHFRA PSKILWRTI RGMIPHKTKR GAAALARLKV YEGVPPPYDK IKRMVIPDAL KVLRLQAGHK YCLLGKLSSE VGWNHYDTIK E LENKRKER ...String:
MVSGSGISAR RIVVDARHHM LGRLSSILAK ELLNGQKVVV VRCEEICLSG GLVRQKMKYL RFLRKRMNTK PSHGPIHFRA PSKILWRTI RGMIPHKTKR GAAALARLKV YEGVPPPYDK IKRMVIPDAL KVLRLQAGHK YCLLGKLSSE VGWNHYDTIK E LENKRKER AQVAYERRKQ LAKLRVKAEK AAEEKLGPQL AIIAPIKY

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Macromolecule #30: uL14 (60S ribosomal protein L23)

MacromoleculeName: uL14 (60S ribosomal protein L23) / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: common tobacco (common tobacco)
Molecular weightTheoretical: 15.040858 KDa
SequenceString:
MSKRGRGGSA GNKFRMSLGL PVAATINCAD NTGAKNLYII SVKGIKGRLN RLPSACVGDM VMATVKKGKP DLRKKVMPAV VVRQRKPWR RKDGVFMYFE DNAGVIVNPK GEMKGSAITG PIGKECADLW PRIASAANAI V

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Macromolecule #31: uL15 (60S ribosomal protein L27a)

MacromoleculeName: uL15 (60S ribosomal protein L27a) / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: common tobacco (common tobacco)
Molecular weightTheoretical: 16.578416 KDa
SequenceString:
MTTRFKKNRK KRGHVSAGHG RVGKHRKHPG GRGNAGGMHH HRILFDKYHP GYFGKVGMRY FHKLRNKFHC PTINIDNLWS LVPQEVKDK AKSSNATAPF IDVTQYGYFK VLGKGVLPQN QAVVVKAKLI SKNAEKKIKE AGGAVVLTA

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Macromolecule #32: uL16 (60S ribosomal protein L10)

MacromoleculeName: uL16 (60S ribosomal protein L10) / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: common tobacco (common tobacco)
Molecular weightTheoretical: 25.01242 KDa
SequenceString: MGRRPARCYR QIKNKPYPKS RFCRGVPDPK IRIYDVGMKK KGVDEFPFCV HLVSWEKENV SSEALEAARI ACNKYMTKSA GKDAFHLRV RVHPFHVLRI NKMLSCAGAD RLQTGMRGAF GKPQGVCARV AIGQVLLSVR CKDGNANHAQ EALRRAKFKF P GRQKIIVS ...String:
MGRRPARCYR QIKNKPYPKS RFCRGVPDPK IRIYDVGMKK KGVDEFPFCV HLVSWEKENV SSEALEAARI ACNKYMTKSA GKDAFHLRV RVHPFHVLRI NKMLSCAGAD RLQTGMRGAF GKPQGVCARV AIGQVLLSVR CKDGNANHAQ EALRRAKFKF P GRQKIIVS RKWGFTKFSR TDYLKYKSEN RIIPDGVNAK LLGCHGRLAA RQPGRAFLEA VGN

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Macromolecule #33: uL18 (60S ribosomal protein L5)

MacromoleculeName: uL18 (60S ribosomal protein L5) / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: common tobacco (common tobacco)
Molecular weightTheoretical: 34.627273 KDa
SequenceString: MAFIKVQKTR AYFKRFQVKF KRRREGKTDY RARNRLINQD KNKYNTPKYR FVVRFTNKDI IAQIVSASIA GDMILASAYA RELPQYGLK VGLTNYAAAY CTGLLLARRV LKKLEMDEEY QGNPEASGED YSVEPAESRR PFRALLDVGL IRTTTGNRVF G ALKGALDG ...String:
MAFIKVQKTR AYFKRFQVKF KRRREGKTDY RARNRLINQD KNKYNTPKYR FVVRFTNKDI IAQIVSASIA GDMILASAYA RELPQYGLK VGLTNYAAAY CTGLLLARRV LKKLEMDEEY QGNPEASGED YSVEPAESRR PFRALLDVGL IRTTTGNRVF G ALKGALDG GLDIPHSEKR FAGFSKDSKQ LDADVHRKYI YGGHVATYMK TLTEDEPEKY QSHFSEYIKQ GLEADDLEEM YK KVHAAIR ADPSPKKSEK QPPKQHKRYN LKKLTYEERK AKLIERLNAL NSAAGNDDDE EDDD

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Macromolecule #34: uL22 (60S ribosomal protein L17)

MacromoleculeName: uL22 (60S ribosomal protein L17) / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: common tobacco (common tobacco)
Molecular weightTheoretical: 20.136207 KDa
SequenceString:
MVKYSKEPDN PTKSCKARGS SLRVHFKNTR ETAHALRKMP LDKAKRYLED VLAHKQAIPF TRFCRGVGRT AQAKNRHSSG QGRWPVKST KFILDLLKNA ESNADVKGLD VDLLFISHIQ VNQAQKQRRR TYRAHGRINP YMSHPCHIEL TLSEKEEYVK K EPETQLAP RKTKGSS

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Macromolecule #35: uL23 (60S ribosomal protein L23a)

MacromoleculeName: uL23 (60S ribosomal protein L23a) / type: protein_or_peptide / ID: 35 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: common tobacco (common tobacco)
Molecular weightTheoretical: 17.321516 KDa
SequenceString:
MAPAKADPSK KSDPKAQAAK VAKAVKSGST LKKKSQKIRT KVTFHRPKTL KKDRNPKYPR ISAPGRNKLD QYGILKYPLT TESAMKKIE DNNTLVFIVD IKADKKKIKD AVKKMYDIQT KKVNTLIRPD GTKKAYVRLT PDYDALDVAN KIGII

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Macromolecule #36: uL24 (60S ribosomal protein L26)

MacromoleculeName: uL24 (60S ribosomal protein L26) / type: protein_or_peptide / ID: 36 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: common tobacco (common tobacco)
Molecular weightTheoretical: 16.748537 KDa
SequenceString:
MKYNPRVSSS RRKSRKAHFT APSSVRRVLM SAPLSSDLRT KYNVRSMPVR KDDEVQVVRG TYKGREGKVV QVYRKKWVIH IERITREKV NGSTVNVGIH PSKVVVTKLR LDKDRKSLLD RKAKGRAAAD KDKGTKFTAE DIMQTVD

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Macromolecule #37: uL29 (60S ribosomal protein L35)

MacromoleculeName: uL29 (60S ribosomal protein L35) / type: protein_or_peptide / ID: 37 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: common tobacco (common tobacco)
Molecular weightTheoretical: 14.352466 KDa
SequenceString:
MARIKVHELR NKTKTELLAQ LKDLKAELAL LRVAKVTGGA PNKLSKIKVV RLSIAQVLTV ISQKQKTALR EAYKNKKYLP LDLRPKKTR AIRKRLTKHQ VSLKTEREKK KEMYFPIRKY AIKV

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Macromolecule #38: uL2 (60S ribosomal protein L8)

MacromoleculeName: uL2 (60S ribosomal protein L8) / type: protein_or_peptide / ID: 38 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: common tobacco (common tobacco)
Molecular weightTheoretical: 28.220734 KDa
SequenceString: MGRVIRAQRK GAGSVFKSHT HHRKGPAKFR SLDYGERNGY LKGVVSEIIH DPGRGAPLAR VTFRHPFRYK HQKELFVAAE GMYTGQFIY CGKKANLMVG NVLALRSIPE GAVVCNVEHK VGDRGVFARC SGDYAIVISH NPDNGTTRIK LPSGAKKIVP S GCRAMIGQ ...String:
MGRVIRAQRK GAGSVFKSHT HHRKGPAKFR SLDYGERNGY LKGVVSEIIH DPGRGAPLAR VTFRHPFRYK HQKELFVAAE GMYTGQFIY CGKKANLMVG NVLALRSIPE GAVVCNVEHK VGDRGVFARC SGDYAIVISH NPDNGTTRIK LPSGAKKIVP S GCRAMIGQ VAGGGRTEKP MLKAGNAYHK YRVKRNCWPK VRGVAMNPVE HPHGGGNHQH IGHASTVRRD APPGQKVGLI AA RRTGRLR GQAAATAAKA DKA

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Macromolecule #39: uL30 (60S ribosomal protein L7)

MacromoleculeName: uL30 (60S ribosomal protein L7) / type: protein_or_peptide / ID: 39 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: common tobacco (common tobacco)
Molecular weightTheoretical: 28.148949 KDa
SequenceString: MGEGGVLVPE SVLKKQKRSE EWALVKKQEL EAAKKKNTEN RKLIYNRAKQ YAKEYEAQER ELIQLKREAR LKGGFYVDPE AKLLFIIRI RGINAMAPQT KKILQLLRLR QIFNGVFLKV NKATVNMLHR VEPYVTYGYP NLKSIRELIY KRGYGKVEKQ R IALTDNSV ...String:
MGEGGVLVPE SVLKKQKRSE EWALVKKQEL EAAKKKNTEN RKLIYNRAKQ YAKEYEAQER ELIQLKREAR LKGGFYVDPE AKLLFIIRI RGINAMAPQT KKILQLLRLR QIFNGVFLKV NKATVNMLHR VEPYVTYGYP NLKSIRELIY KRGYGKVEKQ R IALTDNSV IEQVLGKHGI ICIEDLVHEI ITVGPHFKEA NNFLWPFQLK APLGGLKKKR NHYVEGGDAG NRENYINELI RR MN

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Macromolecule #40: uL3 (60S ribosomal protein L3)

MacromoleculeName: uL3 (60S ribosomal protein L3) / type: protein_or_peptide / ID: 40 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: common tobacco (common tobacco)
Molecular weightTheoretical: 44.507215 KDa
SequenceString: MSHRKFEHPR HGSLGFLPRK RAARHRGKVK AFPKDDPSKP CKLTAFLGYK AGMTHIVRDV EKPGSKLHKK ETCEAVTIIE TPPMVIVGV VGYVKTPRGL RCLNTVWAQH LSEELKRRFY KNWCKSKKKA FLKYSKKYET DEGKKDIQTQ LEKLKKYACV I RVLAHTQI ...String:
MSHRKFEHPR HGSLGFLPRK RAARHRGKVK AFPKDDPSKP CKLTAFLGYK AGMTHIVRDV EKPGSKLHKK ETCEAVTIIE TPPMVIVGV VGYVKTPRGL RCLNTVWAQH LSEELKRRFY KNWCKSKKKA FLKYSKKYET DEGKKDIQTQ LEKLKKYACV I RVLAHTQI RKMKGLKQKK AHLMEIQVNG GTIAQKVDFA YGFFEKQVPV DAVFQKDEMI DIIGVTKGKG YEGVVTRWGV TR LPRKTHR GLRKVACIGA WHPARVSYTV ARAGQNGYHH RTEMNKKVYK LGKAGQESHA AITDFDRTEK DITPMGGFPH YGV VKDDYL LIKGCCVGPK KRVVTLRQSL LNQTSRVALE EIKLKFIDTS SKFGHGRFQT TQEKQKFYGR LKA

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Macromolecule #41: uL4 (60S ribosomal protein L4)

MacromoleculeName: uL4 (60S ribosomal protein L4) / type: protein_or_peptide / ID: 41 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: common tobacco (common tobacco)
Molecular weightTheoretical: 44.706887 KDa
SequenceString: MATAAAIPTT TVQSFENDMA TDGAVPLPAV MKAPIRPDVV TYVHSNISKN ARQPYAVSKK AGHQTSAESW GTGRAVSRIP RVAGGGTHR AGQAAFGNMC RGGRMFAPTK IWRRWHRKIP VNQKRYAVAS AIAASAVPSL VLARGHRIES VPELPLVVSD S VESIEKTS ...String:
MATAAAIPTT TVQSFENDMA TDGAVPLPAV MKAPIRPDVV TYVHSNISKN ARQPYAVSKK AGHQTSAESW GTGRAVSRIP RVAGGGTHR AGQAAFGNMC RGGRMFAPTK IWRRWHRKIP VNQKRYAVAS AIAASAVPSL VLARGHRIES VPELPLVVSD S VESIEKTS NAIKALKQIG AYPDAEKAKD SHAIRPGKGK MRNRRYISRK GPLIVYGTEG AKLVKAFRNI PGVEICHVDR LN LLKLAPG GHLGRFIVWT KSAYEKLDGI YGSFDKASEK KKGYVLPRPK MANADLARII NSDEVQSVVR PIKKDVNKRA TLK KNPLKN LNVLLKLNPY AKTARRMSLL AEAQRVKAKK EKLDKKRHQV TKEEASAIRA AGTSWFKTMI SDSDYTEFEN FTKW LGVTQ

+
Macromolecule #42: uL5 (60S ribosomal protein L11)

MacromoleculeName: uL5 (60S ribosomal protein L11) / type: protein_or_peptide / ID: 42 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: common tobacco (common tobacco)
Molecular weightTheoretical: 20.794178 KDa
SequenceString:
MASEKKLSNP MREIKVQKLV LNISVGESGD RLTRAAKVLE QLSGQSPVFS KARYTVRSFG IRRNEKIACY VTVRGEKAMQ LLESGLKVK EYELLRRNFS ETGCFGFGIQ EHIDLGIKYD PSTGIYGMDF YVVLERPGYR VARRRRCKAR VGIQHRVTKD D AMKWFQVK YEGVILNKSS NIQ

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Macromolecule #43: uL6 (60S ribosomal protein L9)

MacromoleculeName: uL6 (60S ribosomal protein L9) / type: protein_or_peptide / ID: 43 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: common tobacco (common tobacco)
Molecular weightTheoretical: 22.057648 KDa
SequenceString: MKTILSSETM DIPDGVNIKV KAKQIEVEGP RGKLTRNFKH LNLDFQLIKD EETGKKKLKI DAWFGSRKTT AAIRTALSHV ENLITGVTK GYRYKMRFVY AHFPINASIS GGNKAIEIRN FLGEKRVRKV DMLEGVTVVR SEKVKDELVL DGNDIELVSR S AALINQKC ...String:
MKTILSSETM DIPDGVNIKV KAKQIEVEGP RGKLTRNFKH LNLDFQLIKD EETGKKKLKI DAWFGSRKTT AAIRTALSHV ENLITGVTK GYRYKMRFVY AHFPINASIS GGNKAIEIRN FLGEKRVRKV DMLEGVTVVR SEKVKDELVL DGNDIELVSR S AALINQKC HVKNKDIRKF LDGIYVSEKG RIVEEE

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Macromolecule #44: nascent chain

MacromoleculeName: nascent chain / type: protein_or_peptide / ID: 44 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: common tobacco (common tobacco)
Molecular weightTheoretical: 2.060531 KDa
SequenceString:
(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)(UNK) (UNK)(UNK)(UNK) (UNK)(UNK)(UNK)(UNK)(UNK)

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Macromolecule #47: MAGNESIUM ION

MacromoleculeName: MAGNESIUM ION / type: ligand / ID: 47 / Number of copies: 60 / Formula: MG
Molecular weightTheoretical: 24.305 Da

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Macromolecule #48: POTASSIUM ION

MacromoleculeName: POTASSIUM ION / type: ligand / ID: 48 / Number of copies: 94 / Formula: K
Molecular weightTheoretical: 39.098 Da

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Macromolecule #49: ZINC ION

MacromoleculeName: ZINC ION / type: ligand / ID: 49 / Number of copies: 4 / Formula: ZN
Molecular weightTheoretical: 65.409 Da

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Macromolecule #50: SPERMIDINE

MacromoleculeName: SPERMIDINE / type: ligand / ID: 50 / Number of copies: 1 / Formula: SPD
Molecular weightTheoretical: 145.246 Da
Chemical component information

ChemComp-SPD:
SPERMIDINE

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Macromolecule #51: SPERMINE

MacromoleculeName: SPERMINE / type: ligand / ID: 51 / Number of copies: 2 / Formula: SPM
Molecular weightTheoretical: 202.34 Da
Chemical component information

ChemComp-SPM:
SPERMINE

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Macromolecule #52: water

MacromoleculeName: water / type: ligand / ID: 52 / Number of copies: 4406 / Formula: HOH
Molecular weightTheoretical: 18.015 Da
Chemical component information

ChemComp-HOH:
WATER

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 8
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Image recordingFilm or detector model: GATAN K3 (6k x 4k) / Average electron dose: 27.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: OTHER / Imaging mode: OTHER / Nominal defocus max: 2.5 µm / Nominal defocus min: 1.0 µm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

+
Image processing

Startup modelType of model: OTHER
Final reconstructionResolution.type: BY AUTHOR / Resolution: 2.14 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 335291
Initial angle assignmentType: NOT APPLICABLE
Final angle assignmentType: OTHER
FSC plot (resolution estimation)

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