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- EMDB-15674: Cryo-EM structure of the plant 40S subunit -

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Basic information

Entry
Database: EMDB / ID: EMD-15674
TitleCryo-EM structure of the plant 40S subunit
Map dataThe small subunit (40S) of the tobacco ribosome from cytosol
Sample
  • Complex: 40S subunit of the cytosolic 80S ribosome from tobacco
    • Protein or peptide: x 33 types
    • RNA: x 2 types
  • Ligand: x 6 types
KeywordsPlant / 80S / Modifications / RIBOSOME
Function / homology
Function and homology information


endonucleolytic cleavage to generate mature 3'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / translation regulator activity / endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / rescue of stalled ribosome / 90S preribosome / maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / maturation of SSU-rRNA / small-subunit processome / protein kinase C binding / maintenance of translational fidelity ...endonucleolytic cleavage to generate mature 3'-end of SSU-rRNA from (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / translation regulator activity / endonucleolytic cleavage in ITS1 to separate SSU-rRNA from 5.8S rRNA and LSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / rescue of stalled ribosome / 90S preribosome / maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) / maturation of SSU-rRNA / small-subunit processome / protein kinase C binding / maintenance of translational fidelity / modification-dependent protein catabolic process / rRNA processing / protein tag activity / ribosome binding / ribosomal small subunit biogenesis / ribosomal small subunit assembly / small ribosomal subunit / small ribosomal subunit rRNA binding / cytosolic small ribosomal subunit / cytoplasmic translation / rRNA binding / ribosome / protein ubiquitination / structural constituent of ribosome / ribonucleoprotein complex / translation / positive regulation of protein phosphorylation / mRNA binding / ubiquitin protein ligase binding / nucleolus / RNA binding / zinc ion binding / nucleus / metal ion binding / cytoplasm / cytosol
Similarity search - Function
: / Ribosomal protein S26e signature. / : / Ribosomal protein S26e / Ribosomal protein S26e superfamily / Ribosomal protein S26e / Ribosomal protein S21e, conserved site / Ribosomal protein S21e signature. / Ribosomal protein S12e signature. / Ribosomal protein S12e ...: / Ribosomal protein S26e signature. / : / Ribosomal protein S26e / Ribosomal protein S26e superfamily / Ribosomal protein S26e / Ribosomal protein S21e, conserved site / Ribosomal protein S21e signature. / Ribosomal protein S12e signature. / Ribosomal protein S12e / Ribosomal protein S5, eukaryotic/archaeal / Ribosomal protein S19e, conserved site / Ribosomal protein S19e signature. / Small (40S) ribosomal subunit Asc1/RACK1 / Ribosomal protein S2, eukaryotic / Ribosomal protein S21e / Ribosomal protein S21e superfamily / Ribosomal protein S21e / 40S Ribosomal protein S10 / S27a-like superfamily / Ribosomal protein S10, eukaryotic/archaeal / Plectin/S10, N-terminal / Plectin/S10 domain / Ribosomal protein S25 / S25 ribosomal protein / Ribosomal protein S2, eukaryotic/archaeal / : / Ribosomal protein S17e, conserved site / Ribosomal protein S17e signature. / Ribosomal protein S27a / Ribosomal protein S27a / Ribosomal protein S27a / Ribosomal protein S8e subdomain, eukaryotes / Ribosomal protein S30 / Ribosomal protein S30 / 40S ribosomal protein S29/30S ribosomal protein S14 type Z / Ribosomal protein S7e signature. / Ribosomal protein S3, eukaryotic/archaeal / Ribosomal protein S19e / Ribosomal protein S3Ae, conserved site / Ribosomal protein S19e / Ribosomal protein S3Ae signature. / Ribosomal_S19e / Ribosomal protein S27e signature. / Ribosomal protein S4e, N-terminal, conserved site / Ribosomal protein S4e signature. / 40S ribosomal protein S4, C-terminal domain / 40S ribosomal protein S4 C-terminus / Ribosomal protein S19A/S15e / Ribosomal protein S8e, conserved site / Ribosomal protein S8e signature. / Ribosomal protein S17e / Ribosomal protein S17e-like superfamily / Ribosomal S17 / Ribosomal protein S6, eukaryotic / 40S ribosomal protein S1/3, eukaryotes / Ribosomal protein S4e, N-terminal / RS4NT (NUC023) domain / Ribosomal S24e conserved site / Ribosomal protein S24e signature. / 40S ribosomal protein S11, N-terminal / Ribosomal_S17 N-terminal / Ribosomal protein S7e / Ribosomal protein S7e / Ribosomal protein S4, KOW domain / Ribosomal protein S4e / Ribosomal protein S4e, central region / Ribosomal protein S4e, central domain superfamily / Ribosomal family S4e / Ribosomal protein S27, zinc-binding domain superfamily / Ribosomal protein S24e / Ribosomal protein S23, eukaryotic/archaeal / Ribosomal protein S24e / Ribosomal protein S6/S6e/A/B/2, conserved site / Ribosomal protein S6e signature. / Ribosomal protein S27 / Ribosomal protein S27 / Ribosomal protein S8e / Ribosomal protein S17, archaeal/eukaryotic / Ribosomal protein S3Ae / Ribosomal protein S28e conserved site / Ribosomal S3Ae family / Ribosomal protein S28e signature. / Ribosomal S3Ae family / Ribosomal protein S28e / Ribosomal protein S28e / Ribosomal protein S6e / Ribosomal protein S5/S7, eukaryotic/archaeal / Ribosomal protein S6e / Ribosomal protein S6e / Ribosomal protein S13/S15, N-terminal / Ribosomal protein S15P / Ribosomal S13/S15 N-terminal domain / Ribosomal S13/S15 N-terminal domain / Ribosomal protein S4/S9, eukaryotic/archaeal / Ribosomal protein S8e/ribosomal biogenesis NSA2 / Ribosomal protein S8e / Ribosomal protein S14/S29 / Ribosomal protein L7Ae/L30e/S12e/Gadd45 / Ribosomal protein L7Ae/L30e/S12e/Gadd45 family
Similarity search - Domain/homology
40S ribosomal protein S25 / 40S ribosomal protein S14-2 / 40S ribosomal protein S2-3-like / 40S ribosomal protein S30 / 40S ribosomal protein S20-2 / 40S ribosomal protein S27 / Small ribosomal subunit protein uS2 / 40S ribosomal protein S19-3 / 40S ribosomal protein S18 / 40S ribosomal protein S6 ...40S ribosomal protein S25 / 40S ribosomal protein S14-2 / 40S ribosomal protein S2-3-like / 40S ribosomal protein S30 / 40S ribosomal protein S20-2 / 40S ribosomal protein S27 / Small ribosomal subunit protein uS2 / 40S ribosomal protein S19-3 / 40S ribosomal protein S18 / 40S ribosomal protein S6 / 40S ribosomal protein S7 / 40S ribosomal protein S17-like / 40S ribosomal protein S13 / 40S ribosomal protein S24 / 40S ribosomal protein S21 / Guanine nucleotide-binding protein subunit beta-like protein / 40S ribosomal protein S28 / 40S ribosomal protein S16 / 40S ribosomal protein S12 / 40S ribosomal protein S9-2-like / 40S ribosomal protein S11-like / 40S ribosomal protein S5-like / 40S ribosomal protein S3-2-like / Ubiquitin-40S ribosomal protein S27a-like / 40S ribosomal protein S23 / 40S ribosomal protein S26 / 40S ribosomal protein S8 / 40S ribosomal protein S15a-1 / 40S ribosomal protein S15-like / 40S ribosomal protein S10-1-like / 40S ribosomal protein S29 / 40S ribosomal protein S4 / Small ribosomal subunit protein eS1
Similarity search - Component
Biological speciesNicotiana tabacum (common tobacco)
Methodsingle particle reconstruction / cryo EM / Resolution: 2.38 Å
AuthorsSmirnova J / Loerke J / Kleinau G / Schmidt A / Buerger J / Meyer EH / Mielke T / Scheerer P / Bock R / Spahn CMT / Zoschke R
Funding support Germany, 2 items
OrganizationGrant numberCountry
German Research Foundation (DFG)416210002 Germany
German Research Foundation (DFG)INST 335/588-1 Germany
CitationJournal: Nat Plants / Year: 2023
Title: Structure of the actively translating plant 80S ribosome at 2.2 Å resolution.
Authors: Julia Smirnova / Justus Loerke / Gunnar Kleinau / Andrea Schmidt / Jörg Bürger / Etienne H Meyer / Thorsten Mielke / Patrick Scheerer / Ralph Bock / Christian M T Spahn / Reimo Zoschke /
Abstract: In plant cells, translation occurs in three compartments: the cytosol, the plastids and the mitochondria. While the structures of the (prokaryotic-type) ribosomes in plastids and mitochondria are ...In plant cells, translation occurs in three compartments: the cytosol, the plastids and the mitochondria. While the structures of the (prokaryotic-type) ribosomes in plastids and mitochondria are well characterized, high-resolution structures of the eukaryotic 80S ribosomes in the cytosol have been lacking. Here the structure of translating tobacco (Nicotiana tabacum) 80S ribosomes was solved by cryo-electron microscopy with a global resolution of 2.2 Å. The ribosome structure includes two tRNAs, decoded mRNA and the nascent peptide chain, thus providing insights into the molecular underpinnings of the cytosolic translation process in plants. The map displays conserved and plant-specific rRNA modifications and the positions of numerous ionic cofactors, and it uncovers the role of monovalent ions in the decoding centre. The model of the plant 80S ribosome enables broad phylogenetic comparisons that reveal commonalities and differences in the ribosomes of plants and those of other eukaryotes, thus putting our knowledge about eukaryotic translation on a firmer footing.
History
DepositionAug 25, 2022-
Header (metadata) releaseJun 7, 2023-
Map releaseJun 7, 2023-
UpdateApr 24, 2024-
Current statusApr 24, 2024Processing site: PDBe / Status: Released

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Structure visualization

Supplemental images

emd_15674.png

Downloads & links

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Map

FileDownload / File: emd_15674.map.gz / Format: CCP4 / Size: 347.6 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationThe small subunit (40S) of the tobacco ribosome from cytosol
Voxel sizeX=Y=Z: 0.848 Å
Density
Contour LevelBy AUTHOR: 0.426
Minimum - Maximum-6.21226 - 11.349276
Average (Standard dev.)0.010203969 (±0.18939641)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions450450450
Spacing450450450
CellA=B=C: 381.6 Å
α=β=γ: 90.0 °

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Supplemental data

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Mask #1

Fileemd_15674_msk_1.map
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: Half-map A of the tobacco ribosome after local refinement...

Fileemd_15674_half_map_1.map
AnnotationHalf-map_A of the tobacco ribosome after local refinement of the small subunit (40S)
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Half map: Half-map B of the tobacco ribosome after local refinement...

Fileemd_15674_half_map_2.map
AnnotationHalf-map_B of the tobacco ribosome after local refinement of the small subunit (40S)
Projections & Slices
AxesZYX

Projections

Slices (1/2)
Density Histograms

Histogram

Histogram (log scale)

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Sample components

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Entire : 40S subunit of the cytosolic 80S ribosome from tobacco

EntireName: 40S subunit of the cytosolic 80S ribosome from tobacco
Components
  • Complex: 40S subunit of the cytosolic 80S ribosome from tobacco
    • Protein or peptide: eS4 (40S ribosomal protein S4)
    • Protein or peptide: eS6 (40S ribosomal protein S6)
    • RNA: 18S rRNA
    • Protein or peptide: eS10 (40S ribosomal protein S10)
    • Protein or peptide: eS12 (40S ribosomal protein S12)
    • Protein or peptide: eS17 (40S ribosomal protein S17)
    • Protein or peptide: eS19 (40S ribosomal protein S19)
    • Protein or peptide: eS1 (40S ribosomal protein S3a)
    • Protein or peptide: eS21 (40S ribosomal protein S21)
    • Protein or peptide: eS24 (40S ribosomal protein S24)
    • Protein or peptide: eS25 (40S ribosomal protein S25)
    • Protein or peptide: eS26 (40S ribosomal protein S26)
    • Protein or peptide: eS27 (40S ribosomal protein S27)
    • Protein or peptide: eS28 (40S ribosomal protein S28)
    • Protein or peptide: eS30 (40S ribosomal protein S30)
    • Protein or peptide: eS31 (ubiquitin-40S ribosomal protein S27a)
    • Protein or peptide: eS7 (40S ribosomal protein S7)
    • Protein or peptide: eS8 (40S ribosomal protein S8)
    • Protein or peptide: RACK1 (guanine nucleotide-binding protein subunit beta-like protein)
    • Protein or peptide: uS10 (40S ribosomal protein S20)
    • Protein or peptide: uS11 (40S ribosomal protein S14)
    • Protein or peptide: uS12 (40S ribosomal protein S23)
    • Protein or peptide: uS13 (40S ribosomal protein S18)
    • Protein or peptide: uS14 (40S ribosomal protein S29)
    • Protein or peptide: uS15 (40S ribosomal protein S13)
    • Protein or peptide: uS17 (40S ribosomal protein S11)
    • Protein or peptide: uS19 (40S ribosomal protein S15)
    • Protein or peptide: uS2 (40S ribosomal protein SA)
    • Protein or peptide: uS3 (40S ribosomal protein S3)
    • Protein or peptide: uS4 (40S ribosomal protein S9)
    • Protein or peptide: uS5 (40S ribosomal protein S2)
    • Protein or peptide: uS7 (40S ribosomal protein S5)
    • Protein or peptide: uS8 (40S ribosomal protein S15a)
    • Protein or peptide: uS9 (40S ribosomal protein S16)
    • RNA: mRNA
  • Ligand: MAGNESIUM ION
  • Ligand: POTASSIUM ION
  • Ligand: SPERMIDINE
  • Ligand: SPERMINE
  • Ligand: ZINC ION
  • Ligand: water

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Supramolecule #1: 40S subunit of the cytosolic 80S ribosome from tobacco

SupramoleculeName: 40S subunit of the cytosolic 80S ribosome from tobacco
type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#35
Source (natural)Organism: Nicotiana tabacum (common tobacco)

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Macromolecule #1: eS4 (40S ribosomal protein S4)

MacromoleculeName: eS4 (40S ribosomal protein S4) / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Nicotiana tabacum (common tobacco)
Molecular weightTheoretical: 29.971178 KDa
SequenceString: MARGLKKHLK RLNAPKHWML DKLGGAFAPK PSSGPHKSRE CLPLIIILRN RLKYALTYRE VISILMQRQV MVDSKVRTDK TYPAGFMDV VSIPKTNENF RLLYDTKGRF RLHSVRDEEA KFKLCKVRSV QFGQKGIPYL NTYDGRTIRY PDPLIKANDT I KLDLESNK ...String:
MARGLKKHLK RLNAPKHWML DKLGGAFAPK PSSGPHKSRE CLPLIIILRN RLKYALTYRE VISILMQRQV MVDSKVRTDK TYPAGFMDV VSIPKTNENF RLLYDTKGRF RLHSVRDEEA KFKLCKVRSV QFGQKGIPYL NTYDGRTIRY PDPLIKANDT I KLDLESNK IVDFIKFDVG NVVMVTGGRN RGRVGVIKNR EKHKGSFETV HIQDALGHEF ATRLGNVFTL GKGTKPWVSL PK GKGIKLS IIEEARKRLA AQSATTA

UniProtKB: 40S ribosomal protein S4

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Macromolecule #2: eS6 (40S ribosomal protein S6)

MacromoleculeName: eS6 (40S ribosomal protein S6) / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Nicotiana tabacum (common tobacco)
Molecular weightTheoretical: 28.368293 KDa
SequenceString: MKFNIANPTT GCQKKLEIDD DQKLRAFFDK RISQEVSGDA LGDEFKGYVF KIMGGCDKQG FPMKQGVLTP GRVRLLLYRG TPCFRGYGR RNGERRRKSV RGCIVSPDLS VLNLVIVKKG ENDLPGLTDT EKPRMRGPKR ASKIRKLFNL SKEDDVRKYV N TYRRNFTT ...String:
MKFNIANPTT GCQKKLEIDD DQKLRAFFDK RISQEVSGDA LGDEFKGYVF KIMGGCDKQG FPMKQGVLTP GRVRLLLYRG TPCFRGYGR RNGERRRKSV RGCIVSPDLS VLNLVIVKKG ENDLPGLTDT EKPRMRGPKR ASKIRKLFNL SKEDDVRKYV N TYRRNFTT KNGKKASKAP KIQRLVTPLT LQRKRARIAD KKKRIAKAKS EAAEYQKLLA SRLKEQREKR SESLAKKRSR LS AASKPSI AA

UniProtKB: 40S ribosomal protein S6

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Macromolecule #4: eS10 (40S ribosomal protein S10)

MacromoleculeName: eS10 (40S ribosomal protein S10) / type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Nicotiana tabacum (common tobacco)
Molecular weightTheoretical: 23.12141 KDa
SequenceString: MVQSFCCCNR LWLQCDFSPL SPLLPAAMII SEKNRREISK YLFQEGVCYA KKDYNLAKHP LIDVPNLQVI KLMQSFKSKE YVRETFAWM HYYWYLTNDG IEFLRTYLNL PSEIVPATLK KSAKPLGRPM GGPPGDRPRG PPRFEGDRPR FGDREGYRAG P RGPPGEFG ...String:
MVQSFCCCNR LWLQCDFSPL SPLLPAAMII SEKNRREISK YLFQEGVCYA KKDYNLAKHP LIDVPNLQVI KLMQSFKSKE YVRETFAWM HYYWYLTNDG IEFLRTYLNL PSEIVPATLK KSAKPLGRPM GGPPGDRPRG PPRFEGDRPR FGDREGYRAG P RGPPGEFG GEKGGAPADY QPAFRGSGGR PGFGRGSGGF GGAPPSSSFS

UniProtKB: 40S ribosomal protein S10-1-like

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Macromolecule #5: eS12 (40S ribosomal protein S12)

MacromoleculeName: eS12 (40S ribosomal protein S12) / type: protein_or_peptide / ID: 5 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Nicotiana tabacum (common tobacco)
Molecular weightTheoretical: 15.190561 KDa
SequenceString:
MSGEDAAVPV VAAAETPAPA LGEPMDIMTA LQLVLKKSKA HGGLARGLHE GAKVIEKHAA QLCVLAEDCD QPDYVKLVKA LCADHNVSL ITVPNAKTLG EWAGLCKIDS EGKARKVVGC GCVVVKDYGE ETEGLHIVQE YVKSH

UniProtKB: 40S ribosomal protein S12

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Macromolecule #6: eS17 (40S ribosomal protein S17)

MacromoleculeName: eS17 (40S ribosomal protein S17) / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Nicotiana tabacum (common tobacco)
Molecular weightTheoretical: 16.643303 KDa
SequenceString:
MGRVRTKTVK KSSRQVIERY YSKMTLDFHT NKKILEEVAI IPSKRLRNKI AGFSTHLMKR IQKGPVRGIS LKLQEEERER RMDFVPDES AIQTDRIEVD KETIDLLASL GMSELPGVVL KEEQPVAIAA PVGFGGRGGF GGAGGRRGGY

UniProtKB: 40S ribosomal protein S17-like

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Macromolecule #7: eS19 (40S ribosomal protein S19)

MacromoleculeName: eS19 (40S ribosomal protein S19) / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Nicotiana tabacum (common tobacco)
Molecular weightTheoretical: 16.034395 KDa
SequenceString:
MEAARTVKDV SPHEFVKAYA AHLKRSGKME LPEWTDIVKT GKLKELAPYD PDWYYIRAAS MARKIYLRGG LGVGGFRRIY GGNQRNGSR PRHFCKSSGS VARNILQQLQ NMNIVDFDPK GGRRITSNGQ RDLDQVAGRI AAAL

UniProtKB: 40S ribosomal protein S19-3

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Macromolecule #8: eS1 (40S ribosomal protein S3a)

MacromoleculeName: eS1 (40S ribosomal protein S3a) / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Nicotiana tabacum (common tobacco)
Molecular weightTheoretical: 29.782572 KDa
SequenceString: MAVGKNKRIS KGKKGGKKKA ADPYAKKDWY DIKAPSVFDI KNVGKTLVTR TQGTKIASEG LKHRVFEVSL ADLQKDEDQS FRKIRLRAE DVQGKNVLTN FWGMDFTTDK LRSLVKKWQT LIEAHVDVKT TDSYTLRMFC IAFTKKRPNQ QKRTCYAQSS Q IRQIRRKM ...String:
MAVGKNKRIS KGKKGGKKKA ADPYAKKDWY DIKAPSVFDI KNVGKTLVTR TQGTKIASEG LKHRVFEVSL ADLQKDEDQS FRKIRLRAE DVQGKNVLTN FWGMDFTTDK LRSLVKKWQT LIEAHVDVKT TDSYTLRMFC IAFTKKRPNQ QKRTCYAQSS Q IRQIRRKM VEIMRNQASS CDLKELVAKF IPESIGREIE KATSSIFPLQ NVYIRKVKIL KAPKFDIGKL MEVHGDYSED VG VKLDRPA DETVAEAEPE IPGA

UniProtKB: Small ribosomal subunit protein eS1

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Macromolecule #9: eS21 (40S ribosomal protein S21)

MacromoleculeName: eS21 (40S ribosomal protein S21) / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Nicotiana tabacum (common tobacco)
Molecular weightTheoretical: 9.307461 KDa
SequenceString:
MQNEEGQNVD LYIPRKCSAT NRVITSKDHA SVQLNVGHLD DKGLYIPGSF TTFALCGFIR AQGDADSALD RLWQKKKVEA RQQ

UniProtKB: 40S ribosomal protein S21

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Macromolecule #10: eS24 (40S ribosomal protein S24)

MacromoleculeName: eS24 (40S ribosomal protein S24) / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Nicotiana tabacum (common tobacco)
Molecular weightTheoretical: 15.429315 KDa
SequenceString:
MADKAVTIRT RKFMTNRLLA RKQFIIDVLH PGRANVSKAE LKEKLARMYE VKDPNAIFVF KFRTHFGGGK STGFGLIYDS VENAKKYEP KYRLIRNGLD TKVEKSRKQM KERKNRAKKV RGVKKTKAGD AKKK

UniProtKB: 40S ribosomal protein S24

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Macromolecule #11: eS25 (40S ribosomal protein S25)

MacromoleculeName: eS25 (40S ribosomal protein S25) / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Nicotiana tabacum (common tobacco)
Molecular weightTheoretical: 11.795916 KDa
SequenceString:
MAPKKAAPPP SSKPAKSGGG KQKKKKWSKG KQKEKVNNMV LFDKATYDKL LSEAPKYKLI TPSVLSDRLR ISGSLARKAI RDLMARGSI RMVSAHASQQ IYTRATNT

UniProtKB: 40S ribosomal protein S25

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Macromolecule #12: eS26 (40S ribosomal protein S26)

MacromoleculeName: eS26 (40S ribosomal protein S26) / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Nicotiana tabacum (common tobacco)
Molecular weightTheoretical: 14.540069 KDa
SequenceString:
MTFKRRNGGR NKHGRGHTKF IRCSNCGKCC PKDKAIKRFL VRNIVEQAAV RDVQEACAFE TYTLPKLYLK MQYCVSCAIH SKVVRVRSV TDRRVREPPQ RFRRPRDDLP KPGQAPRPTG GAPAAPRT

UniProtKB: 40S ribosomal protein S26

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Macromolecule #13: eS27 (40S ribosomal protein S27)

MacromoleculeName: eS27 (40S ribosomal protein S27) / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Nicotiana tabacum (common tobacco)
Molecular weightTheoretical: 9.613144 KDa
SequenceString:
MTLPNDVDLL NPPAELEKRK HKLKRLVQSP NSFFMDVKCQ GCFNITTVFS HSQTVVVCGN CQTVLCQPTG GRARLTEGCS FRRKGD

UniProtKB: 40S ribosomal protein S27

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Macromolecule #14: eS28 (40S ribosomal protein S28)

MacromoleculeName: eS28 (40S ribosomal protein S28) / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Nicotiana tabacum (common tobacco)
Molecular weightTheoretical: 7.481715 KDa
SequenceString:
MESATKHAIV VKVMGRTGSR GQVTQVRVKF LDDQNRFIMR NVKGPVREGD VLTLLESERE ARRLR

UniProtKB: 40S ribosomal protein S28

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Macromolecule #15: eS30 (40S ribosomal protein S30)

MacromoleculeName: eS30 (40S ribosomal protein S30) / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Nicotiana tabacum (common tobacco)
Molecular weightTheoretical: 6.953253 KDa
SequenceString:
MGKVHGSLAR AGKVRGQTPK VAKQDKKKKP RGRAHKRMQY NRRFVTAVVG FGKKRGPNSS EK

UniProtKB: 40S ribosomal protein S30

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Macromolecule #16: eS31 (ubiquitin-40S ribosomal protein S27a)

MacromoleculeName: eS31 (ubiquitin-40S ribosomal protein S27a) / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Nicotiana tabacum (common tobacco)
Molecular weightTheoretical: 17.739734 KDa
SequenceString:
MQIFVKTLTG KTITLEVESS DTIDNVKAKI QDKEGIPPDQ QRLIFAGKQL EDGRTLADYN IQKESTLHLV LRLRGGAKKR KKKTYTKPK KIKHKKKKVK LAVLQFYKVD DSGKVQRLRK ECPNAECGAG TFMANHFDRH YCGKCGLTYV YQKAGGD

UniProtKB: Ubiquitin-40S ribosomal protein S27a-like

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Macromolecule #17: eS7 (40S ribosomal protein S7)

MacromoleculeName: eS7 (40S ribosomal protein S7) / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Nicotiana tabacum (common tobacco)
Molecular weightTheoretical: 22.121541 KDa
SequenceString:
MYTSRQKIHK DKDAEPTEFE ESVAQALFDM ENTNQELKSE LKDLYINSAV QIDVSGNKKA VVIHVPYRLR KAFRKIHVRL VRELEKKFS GKDVIFIATR RIARPPKRGS AAQRPRSRTL TSVHDAILED VVVPAEIVGK RVRYRVDGSK IMKVFLDPKE R NNTEYKLE TFSAVYRKLS GKDVVFEYPI TEA

UniProtKB: 40S ribosomal protein S7

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Macromolecule #18: eS8 (40S ribosomal protein S8)

MacromoleculeName: eS8 (40S ribosomal protein S8) / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Nicotiana tabacum (common tobacco)
Molecular weightTheoretical: 25.164039 KDa
SequenceString: MGISRDSMHK RRATGGKKKA WRKKRKYELG RQPANTKISA NKTVRRIRVR GGNVKWRALR LDTGNYSWGS EAVTRKTRIL DVVYNASNN ELVRTQTLVK SAIVQVDAAP FKQWYLQHYG VDIGRKKKGP AKKETTEEGE GAAAAAEETK KSNHVLRKIE T RQKDRKLD ...String:
MGISRDSMHK RRATGGKKKA WRKKRKYELG RQPANTKISA NKTVRRIRVR GGNVKWRALR LDTGNYSWGS EAVTRKTRIL DVVYNASNN ELVRTQTLVK SAIVQVDAAP FKQWYLQHYG VDIGRKKKGP AKKETTEEGE GAAAAAEETK KSNHVLRKIE T RQKDRKLD PHIEEQFGGG RLLACISSRP GQCGRADGYI LEGKELEFYM KKLQKKKGKA GAGGAA

UniProtKB: 40S ribosomal protein S8

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Macromolecule #19: RACK1 (guanine nucleotide-binding protein subunit beta-like protein)

MacromoleculeName: RACK1 (guanine nucleotide-binding protein subunit beta-like protein)
type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Nicotiana tabacum (common tobacco)
Molecular weightTheoretical: 36.061844 KDa
SequenceString: MAQESLVLRG TMKAHTDWVT AIATPIDNSD MIVTSSRDKS LIVWSLTKDG PQYGVPRRRL TGHGHFVQDV VLSSDGMFAL SGSWDGELR LWDLQAGTTA RRFVGHTKDV LSVAFSVDNR QIVSASRDKT IKLWNTLGEC KYTIQEGDSH SDWVSCVRFS P NTLQPTIV ...String:
MAQESLVLRG TMKAHTDWVT AIATPIDNSD MIVTSSRDKS LIVWSLTKDG PQYGVPRRRL TGHGHFVQDV VLSSDGMFAL SGSWDGELR LWDLQAGTTA RRFVGHTKDV LSVAFSVDNR QIVSASRDKT IKLWNTLGEC KYTIQEGDSH SDWVSCVRFS P NTLQPTIV SGSWDRTVKI WNLTNCKLRS TLAGHAGYVN TVAVSPDGSL CASGGKDGTI LLWDLAEGKK LYSLDAGSII HA LCFSPNR YWLCAATETS IKIWDLESKS IVVDLKVDLK QESEMATEGT TGSACKNKIM YCTCLSWSAD GSTLFSGYTD GLI RVWGIG RY

UniProtKB: Guanine nucleotide-binding protein subunit beta-like protein

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Macromolecule #20: uS10 (40S ribosomal protein S20)

MacromoleculeName: uS10 (40S ribosomal protein S20) / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Nicotiana tabacum (common tobacco)
Molecular weightTheoretical: 13.651932 KDa
SequenceString:
MAYAAMKATK PGLEEPQEQI HKIRITLSSK NVKNLEKVCA DLVRGAKDKR LRVKGPVRMP TKVLNITTRK SPCGEGTNTW DRFELRVHK RVIDLFSSAD VVKQITSITI EPGVEVEVTI ADS

UniProtKB: 40S ribosomal protein S20-2

+
Macromolecule #21: uS11 (40S ribosomal protein S14)

MacromoleculeName: uS11 (40S ribosomal protein S14) / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Nicotiana tabacum (common tobacco)
Molecular weightTheoretical: 16.375829 KDa
SequenceString:
MSKRRTREPK EETVTLGPAT REGELVFGVA HIFASFNDTF IHVTDLSGRE TMVRITGGMK VKADRDESSP YAAMLAAQDV SQRCKELGI NALHIKLRAT GGNKTKTPGP GAQSALRALA RSGMKIGRIE DVTPIPTDST RRKGGRRGRR L

UniProtKB: 40S ribosomal protein S14-2

+
Macromolecule #22: uS12 (40S ribosomal protein S23)

MacromoleculeName: uS12 (40S ribosomal protein S23) / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Nicotiana tabacum (common tobacco)
Molecular weightTheoretical: 15.771567 KDa
SequenceString:
MGKTRGMGAG RKLKSHRRRQ RWADKSYKKS HLGNEWKKPF AGSSHAKGIV LEKIGIEAKQ PNSAIRKCAR VQLIKNGKKI AAFVPNDGC LNYIEENDEV LIAGFGRKGH AVGDIPGVRF KVVKVSGVSL LALFKEKKEK PRS

UniProtKB: 40S ribosomal protein S23

+
Macromolecule #23: uS13 (40S ribosomal protein S18)

MacromoleculeName: uS13 (40S ribosomal protein S18) / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Nicotiana tabacum (common tobacco)
Molecular weightTheoretical: 17.578473 KDa
SequenceString:
MSLVANEEFQ HILRVQNTNV DGKQKIMFAM TSIKGIGRRF ANIACKKADI DMNKRAGELS AAELDSLMVV VANPRQFKIP DWFLNRQKD YKDGKFSQVT SNALDMKLRD DLERLKKIRN HRGLRHYWGL RVRGQHTKTT GRRGKTVGVS KKR

UniProtKB: 40S ribosomal protein S18

+
Macromolecule #24: uS14 (40S ribosomal protein S29)

MacromoleculeName: uS14 (40S ribosomal protein S29) / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Nicotiana tabacum (common tobacco)
Molecular weightTheoretical: 6.455581 KDa
SequenceString:
MGHSNIWNAH PKNYGPGSRT CRVCGNPHAI IRKYGLMCCR QCFRSNAKEI GFIKYR

UniProtKB: 40S ribosomal protein S29

+
Macromolecule #25: uS15 (40S ribosomal protein S13)

MacromoleculeName: uS15 (40S ribosomal protein S13) / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Nicotiana tabacum (common tobacco)
Molecular weightTheoretical: 17.099254 KDa
SequenceString:
MGRMHSRGKG ISASALPYKR TPPSWLKISA PDVEDNICKF AKKGLTPSQI GVILRDSHGI AQVKSVTGSK ILRILKAHGL APEIPEDLY HLIKKAVAIR KHLERNRKDK DSKFRLILVE SRIHRLARYY KKTKKLPPVW KYESTTASTL VA

UniProtKB: 40S ribosomal protein S13

+
Macromolecule #26: uS17 (40S ribosomal protein S11)

MacromoleculeName: uS17 (40S ribosomal protein S11) / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Nicotiana tabacum (common tobacco)
Molecular weightTheoretical: 17.724816 KDa
SequenceString:
MAEQTEKAFL KQPGVFLSSK KTGKGKRPGK GGNRYFKSIG LGFKTPREAT EGTYIDKKCP FTGNVSIRGR ILAGTCHSAK MNRTIIVRR NYLHYVKKYQ RYEKRHSNIP AHISPCFRVK EGDHVIIGQC RPLSKTVRFN VLKVIPAGSA GGGKKAFTGM

UniProtKB: 40S ribosomal protein S11-like

+
Macromolecule #27: uS19 (40S ribosomal protein S15)

MacromoleculeName: uS19 (40S ribosomal protein S15) / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Nicotiana tabacum (common tobacco)
Molecular weightTheoretical: 17.274441 KDa
SequenceString:
MADVEGADVA AGQPKKRTFK KFSYRGVDLD ALLDMSTDEL VKLFPARPRR RFQRGLKRKP MALIKKLRKA KREAPPGEKP EVVRTHLRN MIIVPEMIGS VIGIYNGKTF NQIEVKPEMI SHYLAEFSIS YKPVKHGRPG IGATHSSRFI PLK

UniProtKB: 40S ribosomal protein S15-like

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Macromolecule #28: uS2 (40S ribosomal protein SA)

MacromoleculeName: uS2 (40S ribosomal protein SA) / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Nicotiana tabacum (common tobacco)
Molecular weightTheoretical: 35.894105 KDa
SequenceString: MAAEGRTLST KEADIQMMLA AEVHLGTKNC DFQMERYVFK RRNDGIYIIN LGKTWEKLQM AARVIVAIEN PKDIIVQSAR PYGQRAVLK FAQYTGANAI AGRHTPGTFT NQLQTSYSEP RLLILTDPRT DHQPIKEAAL GNIPTIAFCD TDSPMRYVDI G IPANNKGK ...String:
MAAEGRTLST KEADIQMMLA AEVHLGTKNC DFQMERYVFK RRNDGIYIIN LGKTWEKLQM AARVIVAIEN PKDIIVQSAR PYGQRAVLK FAQYTGANAI AGRHTPGTFT NQLQTSYSEP RLLILTDPRT DHQPIKEAAL GNIPTIAFCD TDSPMRYVDI G IPANNKGK HSIGVLFWLL ARMVLQMRGS INQGHKWDVM VDLFFYREPE EAKEQQEEEA PAIDYADYSA GGDWSSSQIP EA QWTGDAA PSGPVVASGW SGEGVAEGGG WDTAAAPVPV PVSDAAPTAG GGWDTAAAPV PVPVSDAAPT AGGGWDTAAA PVP VPVSDA APTAGATGWE

UniProtKB: Small ribosomal subunit protein uS2

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Macromolecule #29: uS3 (40S ribosomal protein S3)

MacromoleculeName: uS3 (40S ribosomal protein S3) / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Nicotiana tabacum (common tobacco)
Molecular weightTheoretical: 27.758545 KDa
SequenceString: MDTPVTQISK KRKFVADGVF FAELNEVLTR ELAEDGYSGV EVRVTPVRTE IIIRATRTQN VLGEKGRRIR ELTSVVQKRF KFKENSVEL YAEKVNNRGL CAVAQAESLR YKLLGGLAVR RACYGVLRFI MESGAKGCEV IVSGKLRAQR AKSMKFKDGY M ISSGQPVK ...String:
MDTPVTQISK KRKFVADGVF FAELNEVLTR ELAEDGYSGV EVRVTPVRTE IIIRATRTQN VLGEKGRRIR ELTSVVQKRF KFKENSVEL YAEKVNNRGL CAVAQAESLR YKLLGGLAVR RACYGVLRFI MESGAKGCEV IVSGKLRAQR AKSMKFKDGY M ISSGQPVK EYIDSAVRHI LMRQGVLGIK VKIMLDWDPK GKQGPTTPLP DLVTIHSPKE EEDYIRASVV VPDVVPTMLP TD IPIPVPV M

UniProtKB: 40S ribosomal protein S3-2-like

+
Macromolecule #30: uS4 (40S ribosomal protein S9)

MacromoleculeName: uS4 (40S ribosomal protein S9) / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Nicotiana tabacum (common tobacco)
Molecular weightTheoretical: 23.103805 KDa
SequenceString: MVHVAFYRNY GKTFKKPRRP YEKERLDAEL KLVGEYGLRC KRELWRVQYA LSRIRNAARM LLTLDEKDPR RIFEGEALLR RMNRYGLLD ESQNKLDYVL SLTVENFLER RLQTLVFKTG MAKSIHHARV LIRQRHIRVG RQVVNVPSFM VRLDSQKHID F SLISPFGG ...String:
MVHVAFYRNY GKTFKKPRRP YEKERLDAEL KLVGEYGLRC KRELWRVQYA LSRIRNAARM LLTLDEKDPR RIFEGEALLR RMNRYGLLD ESQNKLDYVL SLTVENFLER RLQTLVFKTG MAKSIHHARV LIRQRHIRVG RQVVNVPSFM VRLDSQKHID F SLISPFGG GRPGRVKRKN QKAAAKKASG GDGDEEDEE

UniProtKB: 40S ribosomal protein S9-2-like

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Macromolecule #31: uS5 (40S ribosomal protein S2)

MacromoleculeName: uS5 (40S ribosomal protein S2) / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Nicotiana tabacum (common tobacco)
Molecular weightTheoretical: 30.652682 KDa
SequenceString: MAERGGERGG FGRGFGGRGG RGGDRGGRGR GGRRPRRETE EEKWVPVTKL GRLVKDNKIR SLEQIYLHSL PIKEFQIIDT LIGPSLKDE VMKIMPVQKQ TRAGQRTRFK AFVVVGDGNG HVGLGVKCSK EVATAIRGAI ILAKLSVIPV RRGYWGNKIG K PHTVPCKV ...String:
MAERGGERGG FGRGFGGRGG RGGDRGGRGR GGRRPRRETE EEKWVPVTKL GRLVKDNKIR SLEQIYLHSL PIKEFQIIDT LIGPSLKDE VMKIMPVQKQ TRAGQRTRFK AFVVVGDGNG HVGLGVKCSK EVATAIRGAI ILAKLSVIPV RRGYWGNKIG K PHTVPCKV TGKCGSVTVR MVPAPRGAGI VAARVPKKVL QFAGIEDVFT SSRGSTKTLG NFVKATFDCL MKTYGFLTPD FW KETRFTK SPFQEYADIL SKPTSKVIIS TTEEAPAPER VEA

UniProtKB: 40S ribosomal protein S2-3-like

+
Macromolecule #32: uS7 (40S ribosomal protein S5)

MacromoleculeName: uS7 (40S ribosomal protein S5) / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Nicotiana tabacum (common tobacco)
Molecular weightTheoretical: 23.373857 KDa
SequenceString: MDAGVVAAPV DASAENKVHS DVMLFNRWTY DDVQINDISV EDYITATASK HPVYMPHTAG RYQAKRFRKA QCPIVERLTN SLMMHGRNN GKKLMAVRII KHAMEIIHLL TDQNPIQVIV DAVINSGPRE DATRIGSAGV VRRQAVDISP LRRVNQAIYL L TTGARESA ...String:
MDAGVVAAPV DASAENKVHS DVMLFNRWTY DDVQINDISV EDYITATASK HPVYMPHTAG RYQAKRFRKA QCPIVERLTN SLMMHGRNN GKKLMAVRII KHAMEIIHLL TDQNPIQVIV DAVINSGPRE DATRIGSAGV VRRQAVDISP LRRVNQAIYL L TTGARESA FRNIKTIAEC LADELINAAK GSSNSYAIKK KDEIERVAKA NR

UniProtKB: 40S ribosomal protein S5-like

+
Macromolecule #33: uS8 (40S ribosomal protein S15a)

MacromoleculeName: uS8 (40S ribosomal protein S15a) / type: protein_or_peptide / ID: 33 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Nicotiana tabacum (common tobacco)
Molecular weightTheoretical: 14.815363 KDa
SequenceString:
MVRVSVLNDA LKSMYNAEKR GKRQVMIRPS SKVIIKFLIV MQKHGYIGEF EYVDDHRSGK IVVELNGRLN KCGVISPRFD VGVKEIEGW TARLLPSRQF GYIVLTTSAG IMDHEEARRK NVGGKVLGFF Y

UniProtKB: 40S ribosomal protein S15a-1

+
Macromolecule #34: uS9 (40S ribosomal protein S16)

MacromoleculeName: uS9 (40S ribosomal protein S16) / type: protein_or_peptide / ID: 34 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Nicotiana tabacum (common tobacco)
Molecular weightTheoretical: 16.731734 KDa
SequenceString:
MNAAPAAVES VQCFGRKKTA VAVTHCKRGR GLIKINGVPI ELVQPEILRY KAFEPILLLG RHRFAGVDMR IRVKGGGHTS QIYAIRQSI AKALVAFYQK YVDEQQKKEI KDILVRYDRT LLVADPRRCE PKKFGGRGAR ARFQKSYR

UniProtKB: 40S ribosomal protein S16

+
Macromolecule #3: 18S rRNA

MacromoleculeName: 18S rRNA / type: rna / ID: 3 / Number of copies: 1
Source (natural)Organism: Nicotiana tabacum (common tobacco)
Molecular weightTheoretical: 583.622188 KDa
SequenceString: UACCUGGUUG AUCCUGCCAG UAGUCAU(A2M)UG CUUGUCU(OMC)AA AGAUUAAGCC AUGCAUGUGU AAGUAUGAAC AA AUUCAGA CUGUGAAACU GCGAA(PSU)GGCU CAU(PSU)AAAUCA GUUAUAGUUU G(PSU)U(OMU)GAUGGU AUCUACU AC U(OMC) ...String:
UACCUGGUUG AUCCUGCCAG UAGUCAU(A2M)UG CUUGUCU(OMC)AA AGAUUAAGCC AUGCAUGUGU AAGUAUGAAC AA AUUCAGA CUGUGAAACU GCGAA(PSU)GGCU CAU(PSU)AAAUCA GUUAUAGUUU G(PSU)U(OMU)GAUGGU AUCUACU AC U(OMC)GGAUAACC GUAGUAAUUC UAG(A2M)GCUAAU ACGUGCAACA AACCCCGACU UCUGGAAGGG AUGCAUUUAU UAGAUAAAA GGUCGACGCG GGCUCUGCCC GUUGCUGC(OMG)A UGAUUCAUGA (PSU)AACUCGACG GAUCGCACGG CCA UCGUGC CGGCGACGCA UCAUUCAAA(PSU) UUC(PSU)GCCCUA UCAACUUUCG AUGGUAGGAU AG(PSU)GGCCUAC CA UGGUGGU GACGGG(PSU)GAC GGAGAAU(OMU)AG GGUUCGAUUC CGGAGA(OMG)GGA GCCUGAGAAA CGGCUACCAC A (PSU)(OMC)CAAGG(A2M) AGGCAGCAGG CGCGC(A2M)AAUU ACCCAA(PSU)CCU GACACGGGGA GGU(A2M)G (PSU)GA(OMC) AAUAAAUAAC AAUACCGGGC UCUAUGAGUC UGGUAAUUGG AAUGAGUACA AUCUAAAUCC CUUAACGA G G(A2M)UCCAUUGG AGGGCAAGUC UGGUGCCAGC AGCCGCGG(OMU)A A(PSU)UCCAGCUC CAAUA(OMG)CGUA (UY1)A(PSU)(PSU)(PSU)AAGU UG(OMU)UGCAGUU (A2M)AAAAGCUCG UAG(PSU)UGGACU UUGGGAUGGG CCG GCCGGU CCGCCUUAGG UGUGCACCGG UCGUCUCGUC CCUUCUGCCG GCGAUGCGCU CCUGGCCUUA AUUGGCCGGG UCGU GCCUC CGGCGCUGUU ACU(PSU)UGAAGA AA(PSU)(PSU)AGAGUG CUCAAAGCAA GCCUACGCUC UGUAUACAUU (A2M)GCAUGGGA (PSU)AACAUUAUA GGAUUUCGGU CCUAUUACGU UGGCCUUCGG GAUCGGAGUA AUGAUUAACA GGG ACAGUC GGGGGCAU(OMU)C GUAUUUCAUA GUCAGAGGUG AAAUUCUUGG AUUUAUGAAA GACGAACAAC UGCGAAAGCA UU(PSU)GCCAAG GAUG(PSU)UUUCA UUAAUCAAGA (A2M)CGAAAGUUG GGGGCUCGAA GACGA(PSU)CAGA UACCG (OMU)CCU AGUCUCAACC A(PSU)AAACGAUG CCGACCAGGG AUCGGCGGAU GUUGCUUUUA GGACUCCGCC GGCACCUU A UGAGAAAUCA AAGUUUUUGG G(PSU)UCCGGGGG GAGUA(PSU)GGUC GCAAGGCUGA AACUUAAAGG AAUUGACGGA A GGGCACCA CCAGGAGUGG AGCC(PSU)GCGGC (PSU)UAAUU(PSU)GAC (B8N)CAACACGGG GAAACU(PSU)ACC AG G(PSU)(OMC)CAGA CAUAGUAAGG A(OMU)UGACAGAC UGAGAGCUCU UUCUUGAUUC UAUGGGUGG(OMU) G(OMG)U GCAUGG C(4AC)GUUCUUAG U(PSU)GGUGGAGC GA(PSU)UUG(PSU)CUG GUUAAUUCCG UUAACGA(A2M)CG AGAC CUCAG CCUGCUAACU AGCUAUGCGG AGGUAUCCCU UCGCGGCCAG CU(OMU)CUUAGAG GGACUACGGC CUUUUAGGCC G CGGAAGUU UGAGGCAAUA ACA(OMG)GUCUGU GAUGCCC(OMU)UA GAUGUUCUGG GCCGCACGCG CGCUACACUG AUGU A(PSU)UCA ACGAGUUUAU AGCCUUGGCC GACAGGCCCG GGUAA(PSU)CUUU GAAAUU(PSU)CAU CGUGAUGGGG AUA GAUCAU UGCAAUUGU(PSU) GGUCUUCAAC G(A2M)GGAAUUCC UAGUAAGCGC GAGUCAUCAG CUCGCGU(PSU)GA CU ACGUCCC UGCCCUU(PSU)GU ACACACCG(OMC)C CGUCGCUCCU ACCGAUUGAA UGAUCCGGUG AAAUGUUCGG AUCGC GGCG ACGUGGGCGG UUCGCUGCCC GCGACGUCGC GAGAAGUCCA UUGAACCUUA UCAUUUAGAG GA(A2M)GGAGAAG UC GUA(6MZ)CAA GGUUUC(4AC)GUA GG(PSU)G(MA6)(MA6)CCUG CGGAAGGAUC AUUG

GENBANK: GENBANK: HQ384692.1

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Macromolecule #35: mRNA

MacromoleculeName: mRNA / type: rna / ID: 35 / Number of copies: 1
Source (natural)Organism: Nicotiana tabacum (common tobacco)
Molecular weightTheoretical: 3.629032 KDa
SequenceString:
UUUUUUUUUU UU

+
Macromolecule #36: MAGNESIUM ION

MacromoleculeName: MAGNESIUM ION / type: ligand / ID: 36 / Number of copies: 76 / Formula: MG
Molecular weightTheoretical: 24.305 Da

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Macromolecule #37: POTASSIUM ION

MacromoleculeName: POTASSIUM ION / type: ligand / ID: 37 / Number of copies: 47 / Formula: K
Molecular weightTheoretical: 39.098 Da

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Macromolecule #38: SPERMIDINE

MacromoleculeName: SPERMIDINE / type: ligand / ID: 38 / Number of copies: 1 / Formula: SPD
Molecular weightTheoretical: 145.246 Da
Chemical component information

ChemComp-SPD:
SPERMIDINE

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Macromolecule #39: SPERMINE

MacromoleculeName: SPERMINE / type: ligand / ID: 39 / Number of copies: 3 / Formula: SPM
Molecular weightTheoretical: 202.34 Da
Chemical component information

ChemComp-SPM:
SPERMINE

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Macromolecule #40: ZINC ION

MacromoleculeName: ZINC ION / type: ligand / ID: 40 / Number of copies: 3 / Formula: ZN
Molecular weightTheoretical: 65.409 Da

+
Macromolecule #41: water

MacromoleculeName: water / type: ligand / ID: 41 / Number of copies: 2372 / Formula: HOH
Molecular weightTheoretical: 18.015 Da
Chemical component information

ChemComp-HOH:
WATER

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Experimental details

-
Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

-
Sample preparation

BufferpH: 8
VitrificationCryogen name: ETHANE

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Electron microscopy

MicroscopeFEI TITAN KRIOS
Image recordingFilm or detector model: GATAN K3 (6k x 4k) / Average electron dose: 27.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: OTHER / Imaging mode: OTHER / Nominal defocus max: 2.5 µm / Nominal defocus min: 1.0 µm
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

+
Image processing

Startup modelType of model: OTHER
Final reconstructionResolution.type: BY AUTHOR / Resolution: 2.38 Å / Resolution method: FSC 0.143 CUT-OFF / Number images used: 335672
Initial angle assignmentType: NOT APPLICABLE
Final angle assignmentType: OTHER
FSC plot (resolution estimation)

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