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-Structure paper
タイトル | Structure snapshots reveal the mechanism of a bacterial membrane lipoprotein -acyltransferase. |
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ジャーナル・号・ページ | Sci Adv, Vol. 9, Issue 26, Page eadf5799, Year 2023 |
掲載日 | 2023年6月30日 |
著者 | Luke Smithers / Oksana Degtjarik / Dietmar Weichert / Chia-Ying Huang / Coilín Boland / Katherine Bowen / Abraham Oluwole / Corinne Lutomski / Carol V Robinson / Eoin M Scanlan / Meitian Wang / Vincent Olieric / Moran Shalev-Benami / Martin Caffrey / |
PubMed 要旨 | Bacterial lipoproteins (BLPs) decorate the surface of membranes in the cell envelope. They function in membrane assembly and stability, as enzymes, and in transport. The final enzyme in the BLP ...Bacterial lipoproteins (BLPs) decorate the surface of membranes in the cell envelope. They function in membrane assembly and stability, as enzymes, and in transport. The final enzyme in the BLP synthesis pathway is the apolipoprotein -acyltransferase, Lnt, which is proposed to act by a ping-pong mechanism. Here, we use x-ray crystallography and cryo-electron microscopy to chart the structural changes undergone during the progress of the enzyme through the reaction. We identify a single active site that has evolved to bind, individually and sequentially, substrates that satisfy structural and chemical criteria to position reactive parts next to the catalytic triad for reaction. This study validates the ping-pong mechanism, explains the molecular bases for Lnt's substrate promiscuity, and should facilitate the design of antibiotics with minimal off-target effects. |
リンク | Sci Adv / PubMed:37390210 / PubMed Central |
手法 | EM (単粒子) / X線回折 |
解像度 | 2.395 - 3.13 Å |
構造データ | EMDB-15786, PDB-8b0k: EMDB-15787, PDB-8b0l: EMDB-15788, PDB-8b0m: EMDB-15789, PDB-8b0n: EMDB-15790, PDB-8b0o: EMDB-15791, PDB-8b0p: PDB-8aq2: PDB-8aq3: PDB-8aq4: |
化合物 | ChemComp-OLC: ChemComp-QGT: ChemComp-FLC: ChemComp-GOL: ChemComp-HOH: ChemComp-6OU: ChemComp-PG5: ChemComp-PG6: ChemComp-TRS: ChemComp-ACT: ChemComp-LMT: ChemComp-CL: ChemComp-PTY: ChemComp-OU9: ChemComp-OJF: ChemComp-IG7: |
由来 |
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キーワード | TRANSFERASE / Lnt / apolipoprotein N-acyltransferase / Bacterial lipoproteins. / bacterial lipoprotein / cryo-EM |