EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Analysis of the conformational heterogeneity of the Rieske iron-sulfur protein in complex III by cryo-EM.
ジャーナル・号・ページ	IUCrJ, Vol. 10, Issue Pt 1, Page 27-37, Year 2023
掲載日	2023年1月1日
著者	Jan Philip Wieferig / Werner Kühlbrandt /
PubMed 要旨	Movement of the Rieske domain of the iron-sulfur protein is essential for intramolecular electron transfer within complex III (CIII) of the respiratory chain as it bridges a gap in the cofactor chain ...Movement of the Rieske domain of the iron-sulfur protein is essential for intramolecular electron transfer within complex III (CIII) of the respiratory chain as it bridges a gap in the cofactor chain towards the electron acceptor cytochrome c. We present cryo-EM structures of CIII from Yarrowia lipolytica at resolutions up to 2.0 Å under different conditions, with different redox states of the cofactors of the high-potential chain. All possible permutations of three primary positions were observed, indicating that the two halves of the dimeric complex act independently. Addition of the substrate analogue decylubiquinone to CIII with a reduced high-potential chain increased the occupancy of the Q site. The extent of Rieske domain interactions through hydrogen bonds to the cytochrome b and cytochrome c subunits varied depending on the redox state and substrate. In the absence of quinols, the reduced Rieske domain interacted more closely with cytochrome b and cytochrome c than in the oxidized state. Upon addition of the inhibitor antimycin A, the heterogeneity of the cd-helix and ef-loop increased, which may be indicative of a long-range effect on the Rieske domain.
リンク	IUCrJ / PubMed:36598500 / PubMed Central
手法	EM (単粒子)
解像度	2.0 - 3.7 Å
構造データ	15312 8ab6 EMDB-15312, PDB-8ab6: Complex III2 from Yarrowia lipolytica, combined datasets, consensus refinement 手法: EM (単粒子) / 解像度: 2.0 Å 15313 8ab7 EMDB-15313, PDB-8ab7: Complex III2 from Yarrowia lipolytica, atovaquone and antimycin A bound 手法: EM (単粒子) / 解像度: 3.3 Å 15314 8ab8 EMDB-15314, PDB-8ab8: Complex III2, b-position, with decylubiquinone and ascorbate-reduced 手法: EM (単粒子) / 解像度: 2.6 Å 15315 8ab9 EMDB-15315, PDB-8ab9: Complex III2 from Yarrowia lipolytica, ascorbate-reduced, b-position 手法: EM (単粒子) / 解像度: 3.3 Å 15316 8aba EMDB-15316, PDB-8aba: Complex III2 from Yarrowia lipolytica, ascorbate-reduced, int-position 手法: EM (単粒子) / 解像度: 3.2 Å 15317 8abb EMDB-15317, PDB-8abb: Complex III2 from Yarrowia lipolytica, ascorbate-reduced, c-position 手法: EM (単粒子) / 解像度: 3.2 Å 15318 8abe EMDB-15318, PDB-8abe: Complex III2 from Yarrowia lipolytica, oxidised with ferricyanide, b-position 手法: EM (単粒子) / 解像度: 2.3 Å 15319 8abf EMDB-15319, PDB-8abf: Complex III2 from Yarrowia lipolytica, oxidised with ferricyanide, int-position 手法: EM (単粒子) / 解像度: 2.3 Å 15320 8abg EMDB-15320, PDB-8abg: Complex III2 from Yarrowia lipolytica, oxidised with ferricyanide, c-position 手法: EM (単粒子) / 解像度: 2.3 Å 15321 8abh EMDB-15321, PDB-8abh: Complex III2 from Yarrowia lipolytica, antimycin A bound, b-position 手法: EM (単粒子) / 解像度: 3.0 Å 15322 8abi EMDB-15322, PDB-8abi: Complex III2 from Yarrowia lipolytica,antimycin A bound, int-position 手法: EM (単粒子) / 解像度: 3.0 Å 15323 8abj EMDB-15323, PDB-8abj: Complex III2 from Yarrowia lipolytica, antimycin A bound, c-position 手法: EM (単粒子) / 解像度: 3.7 Å 15324 8abk EMDB-15324, PDB-8abk: Complex III2 from Yarrowia lipolytica, decylubiquinol bound, b-position 手法: EM (単粒子) / 解像度: 2.5 Å 15325 8abl EMDB-15325, PDB-8abl: Complex III2 from Yarrowia lipolytica, with decylubiquinol and antimycin A, consensus refinement 手法: EM (単粒子) / 解像度: 2.1 Å 15326 8abm EMDB-15326, PDB-8abm: Complex III2 from Yarrowia lipolytica, apo, b-position 手法: EM (単粒子) / 解像度: 2.8 Å 15332 8ac3 EMDB-15332, PDB-8ac3: Complex III2 from Yarrowia lipolytica, apo, int-position 手法: EM (単粒子) / 解像度: 2.8 Å 15333 8ac4 EMDB-15333, PDB-8ac4: Complex III2 from Yarrowia lipolytica, apo, c-position 手法: EM (単粒子) / 解像度: 2.7 Å 15334 8ac5 EMDB-15334, PDB-8ac5: Complex III2 from Yarrowia lipolytica, with decylubiquinol, oxidised, b-position 手法: EM (単粒子) / 解像度: 3.1 Å
化合物	ChemComp-HEM: PROTOPORPHYRIN IX CONTAINING FE ChemComp-PC1: 1,2-DIACYL-SN-GLYCERO-3-PHOSPHOCHOLINE / リン脂質YM ChemComp-PTY: PHOSPHATIDYLETHANOLAMINE / リン脂質YM ChemComp-CDL: CARDIOLIPIN / リン脂質YM ChemComp-LMT: DODECYL-BETA-D-MALTOSIDE / ドデシルβ-D-マルトシド / 可溶化剤YM ChemComp-XP4: 1,2-DIMYRISTOYL-SN-GLYCERO-3-PHOSPHATE / DMPA, リン脂質YM ChemComp-HEC: HEME C ChemComp-HOH: WATER ChemComp-AWB: [(2R,3S,6S,7R,8R)-3-[(3-formamido-2-oxidanyl-phenyl)carbonylamino]-8-hexyl-2,6-dimethyl-4,9-bis(oxidanylidene)-1,5-dioxonan-7-yl] 3-methylbutanoate / アンチマイシンA_1b ChemComp-AOQ: 2-[trans-4-(4-chlorophenyl)cyclohexyl]-3-hydroxynaphthalene-1,4-dione / アトバコン / 薬剤, AntimicrobialYM ChemComp-FES: FE2/S2 (INORGANIC) CLUSTER ChemComp-DCQ: 2-decyl-5,6-dimethoxy-3-methylcyclohexa-2,5-diene-1,4-dione / デシルユビキノン
由来	yarrowia lipolytica (酵母)
キーワード	MEMBRANE PROTEIN / oxidoreductase / electron transport chain / inhibitor