EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Outer membrane utilisomes mediate glycan uptake in gut Bacteroidetes.
ジャーナル・号・ページ	Nature, Vol. 618, Issue 7965, Page 583-589, Year 2023
掲載日	2023年6月7日
著者	Joshua B R White / Augustinas Silale / Matthew Feasey / Tiaan Heunis / Yiling Zhu / Hong Zheng / Akshada Gajbhiye / Susan Firbank / Arnaud Baslé / Matthias Trost / David N Bolam / Bert van den Berg / Neil A Ranson /
PubMed 要旨	Bacteroidetes are abundant members of the human microbiota, utilizing a myriad of diet- and host-derived glycans in the distal gut. Glycan uptake across the bacterial outer membrane of these bacteria ...Bacteroidetes are abundant members of the human microbiota, utilizing a myriad of diet- and host-derived glycans in the distal gut. Glycan uptake across the bacterial outer membrane of these bacteria is mediated by SusCD protein complexes, comprising a membrane-embedded barrel and a lipoprotein lid, which is thought to open and close to facilitate substrate binding and transport. However, surface-exposed glycan-binding proteins and glycoside hydrolases also play critical roles in the capture, processing and transport of large glycan chains. The interactions between these components in the outer membrane are poorly understood, despite being crucial for nutrient acquisition by our colonic microbiota. Here we show that for both the levan and dextran utilization systems of Bacteroides thetaiotaomicron, the additional outer membrane components assemble on the core SusCD transporter, forming stable glycan-utilizing machines that we term utilisomes. Single-particle cryogenic electron microscopy structures in the absence and presence of substrate reveal concerted conformational changes that demonstrate the mechanism of substrate capture, and rationalize the role of each component in the utilisome.
リンク	Nature / PubMed:37286596
手法	EM (単粒子) / X線回折
解像度	2.3 - 3.5 Å
構造データ	15288 8a9y EMDB-15288, PDB-8a9y: Substrate-free levan utilisation machinery (utilisome) 手法: EM (単粒子) / 解像度: 3.5 Å 15289 8aa0 EMDB-15289, PDB-8aa0: Levan utilisation machinery (utilisome) with levan fructo-oligosaccharides DP 8-12 手法: EM (単粒子) / 解像度: 3.2 Å 15290 8aa1 EMDB-15290, PDB-8aa1: Core SusCD transporter units from the levan utilisome with levan fructo-oligosaccharides DP 8-12 手法: EM (単粒子) / 解像度: 2.9 Å 15291 8aa2 EMDB-15291, PDB-8aa2: Inactive levan utilisation machinery (utilisome) in the presence of levan fructo-oligosaccharides DP 15-25 手法: EM (単粒子) / 解像度: 3.1 Å 15292 8aa3 EMDB-15292, PDB-8aa3: Core SusCD transporter units from the inactive levan utilisome in the presence of levan fructo-oligosaccharides DP 15-25 手法: EM (単粒子) / 解像度: 2.7 Å 15293 8aa4 EMDB-15293: Consensus reconstruction of the dextran utilisation system PDB-8aa4: SusC components of the dextran utilisation system (utilisome) 手法: EM (単粒子) / 解像度: 3.1 Å PDB-7znr: Inactive D62N mutant of BT1760 Endo-acting levanase from Bacteroides thetaiotaomicron VPI-5482 手法: X-RAY DIFFRACTION / 解像度: 2.65 Å PDB-7zns: Inactive D62N mutant of BT1760 Endo-acting levanase from Bacteroides thetaiotaomicron VPI-5482 手法: X-RAY DIFFRACTION / 解像度: 2.3 Å
化合物	ChemComp-SO4: SULFATE ION / 硫酸ジアニオン ChemComp-HOH: WATER ChemComp-MG: Unknown entry / マグネシウムジカチオン
由来	bacteroides thetaiotaomicron (バクテリア) bacteroides thetaiotaomicron vpi-5482 (バクテリア) bacteroides thetaiotaomicron (strain atcc 29148 / dsm 2079 / jcm 5827 / ccug 10774 / nctc 10582 / vpi-5482 / e50) (バクテリア)
キーワード	HYDROLASE / Levan / fructan / endo levanase / gut microbiota / TRANSPORT PROTEIN / Membrane protein transporter / glycan transporter / SusCD / utilisome / TonB dependent transporter / TBDT / MEMBRANE PROTEIN