EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Architecture of the AP2/clathrin coat on the membranes of clathrin-coated vesicles.
ジャーナル・号・ページ	Sci Adv, Vol. 6, Issue 30, Page eaba8381, Year 2020
掲載日	2020年7月22日
著者	Oleksiy Kovtun / Veronica Kane Dickson / Bernard T Kelly / David J Owen / John A G Briggs /
PubMed 要旨	Clathrin-mediated endocytosis (CME) is crucial for modulating the protein composition of a cell's plasma membrane. Clathrin forms a cage-like, polyhedral outer scaffold around a vesicle, to which ...Clathrin-mediated endocytosis (CME) is crucial for modulating the protein composition of a cell's plasma membrane. Clathrin forms a cage-like, polyhedral outer scaffold around a vesicle, to which cargo-selecting clathrin adaptors are attached. Adaptor protein complex (AP2) is the key adaptor in CME. Crystallography has shown AP2 to adopt a range of conformations. Here, we used cryo-electron microscopy, tomography, and subtomogram averaging to determine structures, interactions, and arrangements of clathrin and AP2 at the key steps of coat assembly, from AP2 in solution to membrane-assembled clathrin-coated vesicles (CCVs). AP2 binds cargo and PtdIns(4,5) (phosphatidylinositol 4,5-bisphosphate)-containing membranes via multiple interfaces, undergoing conformational rearrangement from its cytosolic state. The binding mode of AP2 β2 appendage into the clathrin lattice in CCVs and buds implies how the adaptor structurally modulates coat curvature and coat disassembly.
リンク	Sci Adv / PubMed:32743075 / PubMed Central
手法	EM (単粒子) / EM (サブトモグラム平均)
解像度	3.9 - 11.9 Å
構造データ	10747 6yae EMDB-10747, PDB-6yae: AP2 core in physiological buffer 手法: EM (単粒子) / 解像度: 3.9 Å 10748 6yaf EMDB-10748, PDB-6yaf: AP2 on a membrane containing tyrosine-based cargo peptide 手法: EM (サブトモグラム平均) / 解像度: 9.1 Å EMDB-10749: AP2 dimer on a membrane containing tyrosine-based cargo peptide 手法: EM (サブトモグラム平均) / 解像度: 10.2 Å EMDB-10750: AP2 in clathrin coats assembled on a membrane containing tyrosine-based cargo peptide 手法: EM (サブトモグラム平均) / 解像度: 11.9 Å 10751 6yah EMDB-10751, PDB-6yah: AP2 in clathrin coats assembled on a membrane containing dileucine- and tyrosine-based cargo peptides 手法: EM (サブトモグラム平均) / 解像度: 10.2 Å EMDB-10752: Hexagon bounding clathrin leg in clathrin coats assembled on a membrane 手法: EM (サブトモグラム平均) / 解像度: 7.7 Å EMDB-10753: Pentagon bounding clathrin legs in clathrin coats assembled on a membrane 手法: EM (サブトモグラム平均) / 解像度: 7.7 Å 10754 6yai EMDB-10754, PDB-6yai: Clathrin with bound beta2 appendage of AP2 手法: EM (サブトモグラム平均) / 解像度: 9.2 Å
由来	rattus norvegicus (ドブネズミ) homo sapiens (ヒト) mus musculus (ハツカネズミ) sus scrofa (ブタ)
キーワード	ENDOCYTOSIS / clathrin / clathrin adaptor / ap2 / clathrin assembly