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-Structure paper
タイトル | Structures of B. subtilis Maturation RNases Captured on 50S Ribosome with Pre-rRNAs. |
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ジャーナル・号・ページ | Mol Cell, Vol. 80, Issue 2, Page 227-236.e5, Year 2020 |
掲載日 | 2020年10月15日 |
著者 | Stephanie Oerum / Tom Dendooven / Marjorie Catala / Laetitia Gilet / Clément Dégut / Aude Trinquier / Maxime Bourguet / Pierre Barraud / Sarah Cianferani / Ben F Luisi / Ciarán Condon / Carine Tisné / |
PubMed 要旨 | The pathways for ribosomal RNA (rRNA) maturation diverge greatly among the domains of life. In the Gram-positive model bacterium, Bacillus subtilis, the final maturation steps of the two large ...The pathways for ribosomal RNA (rRNA) maturation diverge greatly among the domains of life. In the Gram-positive model bacterium, Bacillus subtilis, the final maturation steps of the two large ribosomal subunit (50S) rRNAs, 23S and 5S pre-rRNAs, are catalyzed by the double-strand specific ribonucleases (RNases) Mini-RNase III and RNase M5, respectively. Here we present a protocol that allowed us to solve the 3.0 and 3.1 Å resolution cryoelectron microscopy structures of these RNases poised to cleave their pre-rRNA substrates within the B. subtilis 50S particle. These data provide the first structural insights into rRNA maturation in bacteria by revealing how these RNases recognize and process double-stranded pre-rRNA. Our structures further uncover how specific ribosomal proteins act as chaperones to correctly fold the pre-rRNA substrates and, for Mini-III, anchor the RNase to the ribosome. These r-proteins thereby serve a quality-control function in the process from accurate ribosome assembly to rRNA processing. |
リンク | Mol Cell / PubMed:32991829 / PubMed Central |
手法 | EM (単粒子) / X線回折 |
解像度 | 1.3 - 3.07 Å |
構造データ | EMDB-10535, PDB-6tnn: EMDB-10543, PDB-6tpq: PDB-6tg6: PDB-6tgj: |
化合物 | ChemComp-HOH: ChemComp-MG: ChemComp-ZN: |
由来 |
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キーワード | HYDROLASE / RNase M5 / RNase / rRNA / 5S rRNA / precursor rRNA / pre-5S rRNA / ribosomal RNA / ribonuclease / catalytic domain / toprim domain / RNA BINDING PROTEIN / novel fold / RNA-binding domain / RIBOSOMAL PROTEIN / Mini-RNase III / Mini-III / complex / ribosome / 50S / RNA maturation / RNA processing / precursor 23S rRNA / pre-23S rRNA / 23S rRNA / L3 / RNase III-like fold / M5 / precursor 5S rRNA / L18 |