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-Structure paper
タイトル | Mechanisms of OCT4-SOX2 motif readout on nucleosomes. |
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ジャーナル・号・ページ | Science, Vol. 368, Issue 6498, Page 1460-1465, Year 2020 |
掲載日 | 2020年6月26日 |
著者 | Alicia K Michael / Ralph S Grand / Luke Isbel / Simone Cavadini / Zuzanna Kozicka / Georg Kempf / Richard D Bunker / Andreas D Schenk / Alexandra Graff-Meyer / Ganesh R Pathare / Joscha Weiss / Syota Matsumoto / Lukas Burger / Dirk Schübeler / Nicolas H Thomä / |
PubMed 要旨 | Transcription factors (TFs) regulate gene expression through chromatin where nucleosomes restrict DNA access. To study how TFs bind nucleosome-occupied motifs, we focused on the reprogramming factors ...Transcription factors (TFs) regulate gene expression through chromatin where nucleosomes restrict DNA access. To study how TFs bind nucleosome-occupied motifs, we focused on the reprogramming factors OCT4 and SOX2 in mouse embryonic stem cells. We determined TF engagement throughout a nucleosome at base-pair resolution in vitro, enabling structure determination by cryo-electron microscopy at two preferred positions. Depending on motif location, OCT4 and SOX2 differentially distort nucleosomal DNA. At one position, OCT4-SOX2 removes DNA from histone H2A and histone H3; however, at an inverted motif, the TFs only induce local DNA distortions. OCT4 uses one of its two DNA-binding domains to engage DNA in both structures, reading out a partial motif. These findings explain site-specific nucleosome engagement by the pluripotency factors OCT4 and SOX2, and they reveal how TFs distort nucleosomes to access chromatinized motifs. |
リンク | Science / PubMed:32327602 |
手法 | EM (単粒子) |
解像度 | 3.05 - 3.49 Å |
構造データ | EMDB-10406, PDB-6t90: EMDB-10408, PDB-6t93: EMDB-10864, PDB-6yov: |
化合物 | ChemComp-PTD: |
由来 |
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キーワード | TRANSCRIPTION / nucleosome / OCT4 / SOX2 / transcription factor |