EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Interconversion between Anticipatory and Active GID E3 Ubiquitin Ligase Conformations via Metabolically Driven Substrate Receptor Assembly.
ジャーナル・号・ページ	Mol Cell, Vol. 77, Issue 1, Page 150-163.e9, Year 2020
掲載日	2020年1月2日
著者	Shuai Qiao / Christine R Langlois / Jakub Chrustowicz / Dawafuti Sherpa / Ozge Karayel / Fynn M Hansen / Viola Beier / Susanne von Gronau / Daniel Bollschweiler / Tillman Schäfer / Arno F Alpi / Matthias Mann / J Rajan Prabu / Brenda A Schulman /
PubMed 要旨	Cells respond to environmental changes by toggling metabolic pathways, preparing for homeostasis, and anticipating future stresses. For example, in Saccharomyces cerevisiae, carbon stress-induced ...Cells respond to environmental changes by toggling metabolic pathways, preparing for homeostasis, and anticipating future stresses. For example, in Saccharomyces cerevisiae, carbon stress-induced gluconeogenesis is terminated upon glucose availability, a process that involves the multiprotein E3 ligase GID recruiting N termini and catalyzing ubiquitylation of gluconeogenic enzymes. Here, genetics, biochemistry, and cryoelectron microscopy define molecular underpinnings of glucose-induced degradation. Unexpectedly, carbon stress induces an inactive anticipatory complex (GID), which awaits a glucose-induced substrate receptor to form the active GID. Meanwhile, other environmental perturbations elicit production of an alternative substrate receptor assembling into a related E3 ligase complex. The intricate structure of GID enables anticipating and ultimately binding various N-degron-targeting (i.e., "N-end rule") substrate receptors, while the GID E3 forms a clamp-like structure juxtaposing substrate lysines with the ubiquitylation active site. The data reveal evolutionarily conserved GID complexes as a family of multisubunit E3 ubiquitin ligases responsive to extracellular stimuli.
リンク	Mol Cell / PubMed:31708416
手法	EM (単粒子)
解像度	3.2 - 9.3 Å
構造データ	EMDB-10326: Structure of inactive GID E3 ubiquitin ligase complex 手法: EM (単粒子) / 解像度: 3.7 Å EMDB-10327: Structure of active GID E3 ubiquitin ligase complex 手法: EM (単粒子) / 解像度: 3.8 Å EMDB-10328: Structure of GID Scaffold subcomplex 手法: EM (単粒子) / 解像度: 3.8 Å EMDB-10329: Structure of GID Scaffold subcomplex bound to substrate receptor Gid10 手法: EM (単粒子) / 解像度: 3.8 Å EMDB-10330: Structure of GID Scaffold Subcomplex bound to substrate receptor Gid4 手法: EM (単粒子) / 解像度: 3.4 Å EMDB-10331: Structure of endogenous inactive GID E3 ubiquitin ligase complex 手法: EM (単粒子) / 解像度: 9.3 Å EMDB-10332: Structure of active GID E3 ubiquitin ligase complex with RING domains deletion 手法: EM (単粒子) / 解像度: 7.3 Å 10333 6swy EMDB-10333, PDB-6swy: Structure of active GID E3 ubiquitin ligase complex minus Gid2 and delta Gid9 RING domain 手法: EM (単粒子) / 解像度: 3.2 Å
由来	Saccharomyces cerevisiae (パン酵母) saccharomyces cerevisiae s288c (パン酵母) saccharomyces cerevisiae (strain atcc 204508 / s288c) (パン酵母)
キーワード	LIGASE / Suppressed / Suppreseed