EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Cryo-EM structures of cardiac thin filaments reveal the 3D architecture of troponin.
ジャーナル・号・ページ	J Struct Biol, Vol. 209, Issue 3, Page 107450, Year 2020
掲載日	2020年3月1日
著者	Toshiyuki Oda / Haruaki Yanagisawa / Takeyuki Wakabayashi /
PubMed 要旨	Troponin is an essential component of striated muscle and it regulates the sliding of actomyosin system in a calcium-dependent manner. Despite its importance, the structure of troponin has been ...Troponin is an essential component of striated muscle and it regulates the sliding of actomyosin system in a calcium-dependent manner. Despite its importance, the structure of troponin has been elusive due to its high structural heterogeneity. In this study, we analyzed the 3D structures of murine cardiac thin filaments using a cryo-electron microscope equipped with a Volta phase plate (VPP). Contrast enhancement by a VPP enabled us to reconstruct the entire repeat of the thin filament. We determined the orientation of troponin relative to F-actin and tropomyosin, and characterized the interactions between troponin and tropomyosin. This study provides a structural basis for understanding the molecular mechanism of actomyosin system.
リンク	J Struct Biol / PubMed:31954841
手法	EM (単粒子)
解像度	3.0 - 12.0 Å
構造データ	0711 6kll EMDB-0711, PDB-6kll: F-actin of cardiac thin filament in low-calcium state 手法: EM (単粒子) / 解像度: 3.0 Å 0712 6kln EMDB-0712, PDB-6kln: F-actin of cardiac thin filament in high-calcium state 手法: EM (単粒子) / 解像度: 3.4 Å 0714 6klp EMDB-0714, PDB-6klp: Tropomyosin of cardiac thin filament in low-calcium state 手法: EM (単粒子) / 解像度: 6.6 Å 0715 6klq EMDB-0715, PDB-6klq: Tropomyosin of cardiac thin filament in high-calcium state 手法: EM (単粒子) / 解像度: 7.7 Å 0717 6klt EMDB-0717, PDB-6klt: Troponin of cardiac thin filament in low-calcium state 手法: EM (単粒子) / 解像度: 12.0 Å 0718 6klu EMDB-0718, PDB-6klu: Troponin of cardiac thin filament in high-calcium state 手法: EM (単粒子) / 解像度: 12.0 Å EMDB-0796: F-actin part of cardiac thin filament, actin levels II-IV, low calcium state 手法: EM (単粒子) / 解像度: 3.4 Å EMDB-0797: Tropomyosin in low calcium state, actin levels II-IV 手法: EM (単粒子) / 解像度: 5.3 Å EMDB-0798: F-actin part of cardiac thin filament in low calcium state, actin levels VI-VII, I 手法: EM (単粒子) / 解像度: 3.5 Å EMDB-0799: Tropomyosin of cardiac thin filament in low Ca state, actin levels VI-VII, I 手法: EM (単粒子) / 解像度: 6.6 Å EMDB-0802: F-actin part of cardiac thin filament in high Ca state, actin levels II-IV 手法: EM (単粒子) / 解像度: 5.5 Å EMDB-0804: Tropomyosin in high Ca state, actin levels II-IV 手法: EM (単粒子) / 解像度: 5.5 Å EMDB-0805: F-actin part of cardiac thin filament in high Ca state, actin levels VI-VII, I 手法: EM (単粒子) / 解像度: 3.7 Å EMDB-0806: Tropomyosin in high Ca state, actin levels VI-VII, I 手法: EM (単粒子) / 解像度: 7.4 Å EMDB-0807: Cardiac thin filament in low calcium state 手法: EM (単粒子) / 解像度: 3.2 Å EMDB-0808: Cardiac thin filament in high calcium state 手法: EM (単粒子) / 解像度: 3.4 Å
化合物	ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-ADP: ADENOSINE-5'-DIPHOSPHATE / ADP / ADP, エネルギー貯蔵分子YM ChemComp-CA*: Unknown entry / カルシウムジカチオン
由来	mus musculus (ハツカネズミ) Mouse (ネズミ)
キーワード	CONTRACTILE PROTEIN / Cardiac thin filament