EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structures of a Complete Human V-ATPase Reveal Mechanisms of Its Assembly.
ジャーナル・号・ページ	Mol Cell, Vol. 80, Issue 3, Page 501-511.e3, Year 2020
掲載日	2020年11月5日
著者	Longfei Wang / Di Wu / Carol V Robinson / Hao Wu / Tian-Min Fu /
PubMed 要旨	Vesicular- or vacuolar-type adenosine triphosphatases (V-ATPases) are ATP-driven proton pumps comprised of a cytoplasmic V complex for ATP hydrolysis and a membrane-embedded V complex for proton ...Vesicular- or vacuolar-type adenosine triphosphatases (V-ATPases) are ATP-driven proton pumps comprised of a cytoplasmic V complex for ATP hydrolysis and a membrane-embedded V complex for proton transfer. They play important roles in acidification of intracellular vesicles, organelles, and the extracellular milieu in eukaryotes. Here, we report cryoelectron microscopy structures of human V-ATPase in three rotational states at up to 2.9-Å resolution. Aided by mass spectrometry, we build all known protein subunits with associated N-linked glycans and identify glycolipids and phospholipids in the V complex. We define ATP6AP1 as a structural hub for V complex assembly because it connects to multiple V subunits and phospholipids in the c-ring. The glycolipids and the glycosylated V subunits form a luminal glycan coat critical for V-ATPase folding, localization, and stability. This study identifies mechanisms of V-ATPase assembly and biogenesis that rely on the integrated roles of ATP6AP1, glycans, and lipids.
リンク	Mol Cell / PubMed:33065002 / PubMed Central
手法	EM (単粒子)
解像度	2.9 - 3.6 Å
構造データ	21844 6wlw EMDB-21844, PDB-6wlw: The Vo region of human V-ATPase in state 1 (focused refinement) 手法: EM (単粒子) / 解像度: 3.0 Å 21845 6wlz EMDB-21845, PDB-6wlz: The V1 region of human V-ATPase in state 1 (focused refinement) 手法: EM (単粒子) / 解像度: 2.9 Å 21847 6wm2 EMDB-21847, PDB-6wm2: Human V-ATPase in state 1 with SidK and ADP 手法: EM (単粒子) / 解像度: 3.1 Å 21848 6wm3 EMDB-21848, PDB-6wm3: Human V-ATPase in state 2 with SidK and ADP 手法: EM (単粒子) / 解像度: 3.4 Å 21849 6wm4 EMDB-21849, PDB-6wm4: Human V-ATPase in state 3 with SidK and ADP 手法: EM (単粒子) / 解像度: 3.6 Å
化合物	ChemComp-WSS: tri(methyl)-[2-[[(2~{R})-2-[(~{Z})-octadec-9-enoyl]oxy-3-[(~{E})-1-oxidanylideneoctadec-9-enoxy]propoxy]-oxidanyl-phosphoryl]oxyethyl]azanium ChemComp-PTY: PHOSPHATIDYLETHANOLAMINE / リン脂質YM / ホスファチジルエタノールアミン ChemComp-WJS: (2~{S})-2-$l^{4}-azanyl-3-[[(2~{R})-3-octadecanoyloxy-2-oxidanyl-propoxy]-oxidanyl-oxidanylidene-$l^{6}-phosphanyl]oxy-propanoic acid ChemComp-CLR: CHOLESTEROL / コレステロ-ル / コレステロール ChemComp-PSF: 1,2-DICAPROYL-SN-PHOSPHATIDYL-L-SERINE / 1,2-ジカプロイル-sn-ホスファチジル-L-セリン / ホスファチジルセリン ChemComp-WJP: methyl (3R,6Z,10E,14E)-3,7,11,15,19-pentamethylicosa-6,10,14,18-tetraen-1-yl dihydrogen diphosphate ChemComp-NAG: 2-acetamido-2-deoxy-beta-D-glucopyranose / N-アセチル-β-D-グルコサミン / N-アセチルグルコサミン ChemComp-ADP: ADENOSINE-5'-DIPHOSPHATE / ADP / ADP, エネルギー貯蔵分子YM / アデノシン二リン酸
由来	homo sapiens (ヒト) Human (ヒト) Legionella pneumophila subsp. pneumophila (レジオネラ・ニューモフィラ) legionella pneumophila subsp. pneumophila (strain philadelphia 1 / atcc 33152 / dsm 7513) (レジオネラ・ニューモフィラ) legionella pneumophila (レジオネラ・ニューモフィラ)
キーワード	MEMBRANE PROTEIN (膜タンパク質) / V-ATPase / proton pump (プロトンポンプ) / pump (ポンプ)