EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	Structures and operating principles of the replisome.
ジャーナル・号・ページ	Science, Vol. 363, Issue 6429, Year 2019
掲載日	2019年2月22日
著者	Yang Gao / Yanxiang Cui / Tara Fox / Shiqiang Lin / Huaibin Wang / Natalia de Val / Z Hong Zhou / Wei Yang /
PubMed 要旨	Visualization in atomic detail of the replisome that performs concerted leading- and lagging-DNA strand synthesis at a replication fork has not been reported. Using bacteriophage T7 as a model ...Visualization in atomic detail of the replisome that performs concerted leading- and lagging-DNA strand synthesis at a replication fork has not been reported. Using bacteriophage T7 as a model system, we determined cryo-electron microscopy structures up to 3.2-angstroms resolution of helicase translocating along DNA and of helicase-polymerase-primase complexes engaging in synthesis of both DNA strands. Each domain of the spiral-shaped hexameric helicase translocates sequentially hand-over-hand along a single-stranded DNA coil, akin to the way AAA+ ATPases (adenosine triphosphatases) unfold peptides. Two lagging-strand polymerases are attached to the primase, ready for Okazaki fragment synthesis in tandem. A β hairpin from the leading-strand polymerase separates two parental DNA strands into a T-shaped fork, thus enabling the closely coupled helicase to advance perpendicular to the downstream DNA duplex. These structures reveal the molecular organization and operating principles of a replisome.
リンク	Science / PubMed:30679383 / PubMed Central
手法	EM (単粒子)
解像度	3.2 - 13.8 Å
構造データ	0357 6n7i EMDB-0357, PDB-6n7i: Structure of bacteriophage T7 E343Q mutant gp4 helicase-primase in complex with ssDNA, dTTP, AC dinucleotide and CTP (gp4(5)-DNA) 手法: EM (単粒子) / 解像度: 3.2 Å 0359 6n7n EMDB-0359, PDB-6n7n: Structure of bacteriophage T7 E343Q mutant gp4 helicase-primase in complex with ssDNA, dTTP, AC dinucleotide and CTP (form I) 手法: EM (単粒子) / 解像度: 3.5 Å 0362 6n7s EMDB-0362, PDB-6n7s: Structure of bacteriophage T7 E343Q mutant gp4 helicase-primase in complex with ssDNA, dTTP, AC dinucleotide and CTP (form II) 手法: EM (単粒子) / 解像度: 4.6 Å 0363 6n7t EMDB-0363, PDB-6n7t: Structure of bacteriophage T7 E343Q mutant gp4 helicase-primase in complex with ssDNA, dTTP, AC dinucleotide and CTP (form III) 手法: EM (単粒子) / 解像度: 3.9 Å 0364 6n7v EMDB-0364, PDB-6n7v: Structure of bacteriophage T7 gp4 (helicase-primase, E343Q mutant) in complex with ssDNA, dTTP, AC dinucleotide, and CTP (from multiple lead complexes) 手法: EM (単粒子) / 解像度: 3.8 Å 0365 6n7w EMDB-0365, PDB-6n7w: Structure of bacteriophage T7 leading-strand DNA polymerase (D5A/E7A)/Trx in complex with a DNA fork and incoming dTTP (from multiple lead complexes) 手法: EM (単粒子) / 解像度: 4.5 Å 0379 6n9u EMDB-0379, PDB-6n9u: Structure of bacteriophage T7 lagging-strand DNA polymerase (D5A/E7A) interacting with primase domains of two gp4 subunits bound to an RNA/DNA hybrid and dTTP (from LagS1) 手法: EM (単粒子) / 解像度: 3.7 Å 0380 6n9v EMDB-0380, PDB-6n9v: Structure of bacteriophage T7 lagging-strand DNA polymerase (D5A/E7A) and gp4 (helicase/primase) bound to DNA including RNA/DNA hybrid, and an incoming dTTP (LagS1) 手法: EM (単粒子) / 解像度: 4.0 Å 0381 6n9w EMDB-0381, PDB-6n9w: Structure of bacteriophage T7 lagging-strand DNA polymerase (D5A/E7A) and gp4 (helicase/primase) bound to DNA including RNA/DNA hybrid, and an incoming dTTP (LagS2) 手法: EM (単粒子) / 解像度: 4.0 Å 0382 6n9x EMDB-0382, PDB-6n9x: Structure of bacteriophage T7 lagging-strand DNA polymerase (D5A/E7A) and gp4 (helicase/primase) bound to DNA including RNA/DNA hybrid, and an incoming dTTP (LagS3) 手法: EM (単粒子) / 解像度: 4.1 Å EMDB-0386: Structure of two bacteriophage T7 lagging-strand DNA polymerase (D5A/E7A )/Trx interacting with primase domains, one Pol with RNA/DNA hybrid, and dTTP interacting and the second Pol in apo form (LagS4) 手法: EM (単粒子) / 解像度: 8.6 Å EMDB-0387: Structure of bacteriophage T7 lagging-strand DNA polymerase (D5A/E7A)/Trx interacting with primase domains of two gp4 subunits (E and F), with gp4 helicase bound to a DNA fork and dTTP (LagL1) 手法: EM (単粒子) / 解像度: 6.6 Å EMDB-0388: Structure of bacteriophage T7 lagging-strand DNA polymerase (D5A/E7A)/Trx interacting with primase domains of two gp4 subunits (C and D), with gp4 helicase bound to a DNA fork and dTTP (LagL2) 手法: EM (単粒子) / 解像度: 7.1 Å EMDB-0389: Structure of bacteriophage T7 lagging-strand DNA polymerase (D5A/E7A)/Trx interacting with primase domains of two gp4 subunits (B and C), with gp4 helicase bound to a DNA fork and dTTP (LagL3) 手法: EM (単粒子) / 解像度: 7.8 Å EMDB-0390: Structure of bacteriophage T7 lagging-strand DNA polymerase (D5A/E7A)/Trx interacting with primase domains of two gp4 subunits (A and B), with gp4 helicase bound to a DNA fork and dTTP (LagL4) 手法: EM (単粒子) / 解像度: 8.3 Å EMDB-0391: Structure of bacteriophage T7 leading-strand DNA polymerase (D5A/E7A)/Trx and of gp4 (E343Q) bound to a DNA fork (Lead1) 手法: EM (単粒子) / 解像度: 11.2 Å EMDB-0392: Structure of bacteriophage T7 leading-strand DNA polymerase (D5A/E7A)/Trx and of gp4 (E343Q) bound to a DNA fork (Lead2) 手法: EM (単粒子) / 解像度: 13.3 Å EMDB-0393: Structure of bacteriophage T7 leading-strand DNA polymerase (D5A/E7A)/Trx and of T7 gp4 (E343Q) bound to a DNA fork, and dTTP (Lead3) 手法: EM (単粒子) / 解像度: 11.8 Å EMDB-0394: Structure of bacteriophage T7 leading-strand DNA polymerase (D5A/E7A)/Trx and of gp4 (E343Q) bound to a DNA fork (Lead4) 手法: EM (単粒子) / 解像度: 9.6 Å EMDB-0395: Structure of bacteriophage T7 leading-strand DNA polymerase (D5A/E7A)/Trx and of gp4 (E343Q) bound to a DNA fork (Lead5) 手法: EM (単粒子) / 解像度: 13.8 Å
化合物	ChemComp-TTP: THYMIDINE-5'-TRIPHOSPHATE / dTTP / チミジン三リン酸 ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-HOH: WATER / 水 ChemComp-ZN: Unknown entry
由来	enterobacteria phage t7 (ファージ) escherichia coli (大腸菌)
キーワード	HYDROLASE (加水分解酵素) / TRANSFERASE/DNA / helicase (ヘリカーゼ) / ATPase (ATPアーゼ) / hexamer (オリゴマー) / DNA replication (DNA複製) / TRANSFERASE-DNA complex / DNA polymerase (DNAポリメラーゼ) / replisome / primase (DNAプライマーゼ)