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Structure paper

タイトル	Atypical chemoreceptor arrays accommodate high membrane curvature.
ジャーナル・号・ページ	Nat Commun, Vol. 11, Issue 1, Page 5763, Year 2020
掲載日	2020年11月13日
著者	Alise R Muok / Davi R Ortega / Kurni Kurniyati / Wen Yang / Zachary A Maschmann / Adam Sidi Mabrouk / Chunhao Li / Brian R Crane / Ariane Briegel /
PubMed 要旨	The prokaryotic chemotaxis system is arguably the best-understood signaling pathway in biology. In all previously described species, chemoreceptors organize into a hexagonal (P6 symmetry) extended ...The prokaryotic chemotaxis system is arguably the best-understood signaling pathway in biology. In all previously described species, chemoreceptors organize into a hexagonal (P6 symmetry) extended array. Here, we report an alternative symmetry (P2) of the chemotaxis apparatus that emerges from a strict linear organization of the histidine kinase CheA in Treponema denticola cells, which possesses arrays with the highest native curvature investigated thus far. Using cryo-ET, we reveal that Td chemoreceptor arrays assume an unusual arrangement of the supra-molecular protein assembly that has likely evolved to accommodate the high membrane curvature. The arrays have several atypical features, such as an extended dimerization domain of CheA and a variant CheW-CheR-like fusion protein that is critical for maintaining an ordered chemosensory apparatus. Furthermore, the previously characterized Td oxygen sensor ODP influences CheA ordering. These results suggest a greater diversity of the chemotaxis signaling system than previously thought.
リンク	Nat Commun / PubMed:33188180 / PubMed Central
手法	EM (サブトモグラム平均) / EM (トモグラフィー) / X線回折
解像度	1.5 - 46.0 Å
構造データ	EMDB-11381: Treponema denticola chemotaxis signalling arrays in an ODP (TDE2498) deletion mutant 手法: EM (サブトモグラム平均) / 解像度: 28.2 Å EMDB-11384: Treponema denticola chemotaxis signalling arrays in an ODP (TDE2498) and TDE2496 deletion mutant 手法: EM (サブトモグラム平均) / 解像度: 25.0 Å EMDB-11385: Treponema denticola chemotaxis signalling arrays 手法: EM (サブトモグラム平均) / 解像度: 16.0 Å EMDB-11386: Treponema denticola chemotaxis signalling arrays 手法: EM (トモグラフィー) / 解像度: 46.0 Å PDB-6y1y: CheA dimerization domain of Treponema denticola 手法: X-RAY DIFFRACTION / 解像度: 1.5 Å
化合物	ChemComp-HOH: WATER
由来	treponema denticola (バクテリア)
キーワード	SIGNALING PROTEIN / Histidine kinase / dimerization domain / coiled-coil