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キーワード
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

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Structure paper

タイトル	Electron cryo-microscopy of bacteriophage PR772 reveals the elusive vertex complex and the capsid architecture.
ジャーナル・号・ページ	Elife, Vol. 8, Year 2019
掲載日	2019年9月12日
著者	Hemanth Kn Reddy / Marta Carroni / Janos Hajdu / Martin Svenda /
PubMed 要旨	Bacteriophage PR772, a member of the family, has a 70 nm diameter icosahedral protein capsid that encapsulates a lipid membrane, dsDNA, and various internal proteins. An icosahedrally averaged ...Bacteriophage PR772, a member of the family, has a 70 nm diameter icosahedral protein capsid that encapsulates a lipid membrane, dsDNA, and various internal proteins. An icosahedrally averaged CryoEM reconstruction of the wild-type virion and a localized reconstruction of the vertex region reveal the composition and the structure of the vertex complex along with new protein conformations that play a vital role in maintaining the capsid architecture of the virion. The overall resolution of the virion is 2.75 Å, while the resolution of the protein capsid is 2.3 Å. The conventional penta-symmetron formed by the capsomeres is replaced by a large vertex complex in the pseudo T = 25 capsid. All the vertices contain the host-recognition protein, P5; two of these vertices show the presence of the receptor-binding protein, P2. The 3D structure of the vertex complex shows interactions with the viral membrane, indicating a possible mechanism for viral infection.
リンク	Elife / PubMed:31513011 / PubMed Central
手法	EM (単粒子)
解像度	2.75 - 10.0 Å
構造データ	EMDB-10237: Localized Reconstruction of the vertex region of Bacteriophage PR772 showing the heteropentameric penton 手法: EM (単粒子) / 解像度: 4.41 Å EMDB-10238: Focused Classification of the vertex region of Bacteriophage PR772 showing the heteropentameric penton 手法: EM (単粒子) / 解像度: 4.25 Å 4461 6q5u EMDB-4461, PDB-6q5u: High resolution electron cryo-microscopy structure of the bacteriophage PR772 手法: EM (単粒子) / 解像度: 2.75 Å EMDB-4462: Vertex Complex of Bacteriophage PR772 手法: EM (単粒子) / 解像度: 10.0 Å
由来	enterobacteria phage pr772 (ファージ)
キーワード	VIRUS / Phage / Tectiviridae / Membrane / double-barrel / helix turn helix / helix with a kink / beta-propeller / heteropentamer / penton