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Structure paper

タイトル	Atomic structures of an entire contractile injection system in both the extended and contracted states.
ジャーナル・号・ページ	Nat Microbiol, Vol. 4, Issue 11, Page 1885-1894, Year 2019
掲載日	2019年8月5日
著者	Ambroise Desfosses / Hariprasad Venugopal / Tapan Joshi / Jan Felix / Matthew Jessop / Hyengseop Jeong / Jaekyung Hyun / J Bernard Heymann / Mark R H Hurst / Irina Gutsche / Alok K Mitra /
PubMed 要旨	Contractile injection systems are sophisticated multiprotein nanomachines that puncture target cell membranes. Although the number of atomic-resolution insights into contractile bacteriophage tails, ...Contractile injection systems are sophisticated multiprotein nanomachines that puncture target cell membranes. Although the number of atomic-resolution insights into contractile bacteriophage tails, bacterial type six secretion systems and R-pyocins is rapidly increasing, structural information on the contraction of bacterial phage-like protein-translocation structures directed towards eukaryotic hosts is scarce. Here, we characterize the antifeeding prophage AFP from Serratia entomophila by cryo-electron microscopy. We present the high-resolution structure of the entire AFP particle in the extended state, trace 11 protein chains de novo from the apical cap to the needle tip, describe localization variants and perform specific structural comparisons with related systems. We analyse inter-subunit interactions and highlight their universal conservation within contractile injection systems while revealing the specificities of AFP. Furthermore, we provide the structure of the AFP sheath-baseplate complex in a contracted state. This study reveals atomic details of interaction networks that accompany and define the contraction mechanism of toxin-delivery tailocins, offering a comprehensive framework for understanding their mode of action and for their possible adaptation as biocontrol agents.
リンク	Nat Microbiol / PubMed:31384001 / PubMed Central
手法	EM (単粒子) / EM (らせん対称)
解像度	2.8 - 5.4 Å
構造データ	4782 6rao EMDB-4782, PDB-6rao: Cryo-EM structure of the anti-feeding prophage (AFP) baseplate, 6-fold symmetrised 手法: EM (単粒子) / 解像度: 3.1 Å EMDB-4783: Composite map of an entire contractile injection device : the anti-feeding prophage (AFP) 手法: EM (単粒子) / 解像度: 3.2 Å 4784 6rap EMDB-4784, PDB-6rap: Cryo-EM structure of the anti-feeding prophage cap (AFP tube terminating cap) 手法: EM (単粒子) / 解像度: 3.3 Å 4800 6rbk EMDB-4800, PDB-6rbk: Cryo-EM structure of the anti-feeding prophage (AFP) baseplate in extended state, 3-fold symmetrised 手法: EM (単粒子) / 解像度: 3.4 Å EMDB-4801: Cryo-EM structure of the anti-feeding prophage cap (AFP tube terminating cap), ending with Afp3 手法: EM (単粒子) / 解像度: 3.4 Å 4802 6rbn EMDB-4802, PDB-6rbn: Cryo-EM structure of the anti-feeding prophage (AFP) helical sheath-tube complex in extended state 手法: EM (らせん対称) / 解像度: 2.8 Å 4803 6rc8 EMDB-4803, PDB-6rc8: Cryo-EM structure of the anti-feeding prophage (AFP) helical sheath in contracted state 手法: EM (らせん対称) / 解像度: 3.8 Å EMDB-4859: Cryo-EM structure of the anti-feeding prophage (AFP) sheath-tube in contracted state, C6 symmetrized 手法: EM (単粒子) / 解像度: 4.2 Å EMDB-4871: Cryo-EM structure of the anti-feeding prophage (AFP) needle from signal-subtracted particles 手法: EM (単粒子) / 解像度: 3.7 Å 4876 6rgl EMDB-4876, PDB-6rgl: Cryo-EM structure of the anti-feeding prophage (AFP) baseplate in contracted state 手法: EM (単粒子) / 解像度: 5.4 Å
由来	serratia entomophila (バクテリア)
キーワード	VIRUS LIKE PARTICLE / Anti-feeding prophage / secretion system / AFP / contractile / cap / tube terminating protein / baseplate / sheath / tube / contracted