+
検索条件

キーワード
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

-
Structure paper

タイトル	Activation of the Endonuclease that Defines mRNA 3' Ends Requires Incorporation into an 8-Subunit Core Cleavage and Polyadenylation Factor Complex.
ジャーナル・号・ページ	Mol Cell, Vol. 73, Issue 6, Page 1217-1231.e11, Year 2019
掲載日	2019年3月21日
著者	Chris H Hill / Vytautė Boreikaitė / Ananthanarayanan Kumar / Ana Casañal / Peter Kubík / Gianluca Degliesposti / Sarah Maslen / Angelica Mariani / Ottilie von Loeffelholz / Mathias Girbig / Mark Skehel / Lori A Passmore /
PubMed 要旨	Cleavage and polyadenylation factor (CPF/CPSF) is a multi-protein complex essential for formation of eukaryotic mRNA 3' ends. CPF cleaves pre-mRNAs at a specific site and adds a poly(A) tail. The ...Cleavage and polyadenylation factor (CPF/CPSF) is a multi-protein complex essential for formation of eukaryotic mRNA 3' ends. CPF cleaves pre-mRNAs at a specific site and adds a poly(A) tail. The cleavage reaction defines the 3' end of the mature mRNA, and thus the activity of the endonuclease is highly regulated. Here, we show that reconstitution of specific pre-mRNA cleavage with recombinant yeast proteins requires incorporation of the Ysh1 endonuclease into an eight-subunit "CPF" complex. Cleavage also requires the accessory cleavage factors IA and IB, which bind substrate pre-mRNAs and CPF, likely facilitating assembly of an active complex. Using X-ray crystallography, electron microscopy, and mass spectrometry, we determine the structure of Ysh1 bound to Mpe1 and the arrangement of subunits within CPF. Together, our data suggest that the active mRNA 3' end processing machinery is a dynamic assembly that is licensed to cleave only when all protein factors come together at the polyadenylation site.
リンク	Mol Cell / PubMed:30737185 / PubMed Central
手法	EM (単粒子) / X線回折
解像度	2.28 - 25.0 Å
構造データ	EMDB-0324: Negative-stain map of CPFcore 手法: EM (単粒子) / 解像度: 25.0 Å EMDB-0325: Cryo-EM map of the Ysh1-Mpe1 nuclease complex 手法: EM (単粒子) / 解像度: 6.0 Å PDB-6i1d: Structure of the Ysh1-Mpe1 nuclease complex from S.cerevisiae 手法: X-RAY DIFFRACTION / 解像度: 2.28 Å
化合物	ChemComp-ZN: Unknown entry ChemComp-GOL: GLYCEROL / グリセロ-ル / グリセリン ChemComp-HOH: WATER / 水
由来	Saccharomyces cerevisiae (パン酵母) saccharomyces cerevisiae (strain atcc 204508 / s288c) (パン酵母)
キーワード	GENE REGULATION (遺伝子発現の調節) / pre-mRNA (一次転写産物) / mRNA (伝令RNA) / nuclease (ヌクレアーゼ) / endonuclease (エンドヌクレアーゼ) / cleavage / polyadenylation (ポリアデニル化) / polyA / CPF / metallo-beta-lactamase / 3' ends