+
検索条件

キーワード
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

-
Structure paper

タイトル	Structural principles of SNARE complex recognition by the AAA+ protein NSF.
ジャーナル・号・ページ	Elife, Vol. 7, Year 2018
掲載日	2018年9月10日
著者	K Ian White / Minglei Zhao / Ucheor B Choi / Richard A Pfuetzner / Axel T Brunger /
PubMed 要旨	The recycling of SNARE proteins following complex formation and membrane fusion is an essential process in eukaryotic trafficking. A highly conserved AAA+ protein, NSF (-ethylmaleimide sensitive ...The recycling of SNARE proteins following complex formation and membrane fusion is an essential process in eukaryotic trafficking. A highly conserved AAA+ protein, NSF (-ethylmaleimide sensitive factor) and an adaptor protein, SNAP (soluble NSF attachment protein), disassemble the SNARE complex. We report electron-cryomicroscopy structures of the complex of NSF, αSNAP, and the full-length soluble neuronal SNARE complex (composed of syntaxin-1A, synaptobrevin-2, SNAP-25A) in the presence of ATP under non-hydrolyzing conditions at ~3.9 Å resolution. These structures reveal electrostatic interactions by which two αSNAP molecules interface with a specific surface of the SNARE complex. This interaction positions the SNAREs such that the 15 N-terminal residues of SNAP-25A are loaded into the D1 ring pore of NSF via a spiral pattern of interactions between a conserved tyrosine NSF residue and SNAP-25A backbone atoms. This loading process likely precedes ATP hydrolysis. Subsequent ATP hydrolysis then drives complete disassembly.
リンク	Elife / PubMed:30198481 / PubMed Central
手法	EM (単粒子)
解像度	3.8 - 4.4 Å
構造データ	9100 6mdm EMDB-9100, PDB-6mdm: The 20S supercomplex engaging the SNAP-25 N-terminus (class 1) 手法: EM (単粒子) / 解像度: 4.4 Å 9101 6mdn EMDB-9101, PDB-6mdn: The 20S supercomplex engaging the SNAP-25 N-terminus (class 2) 手法: EM (単粒子) / 解像度: 4.4 Å 9102 6mdo EMDB-9102, PDB-6mdo: The D1 and D2 domain rings of NSF engaging the SNAP-25 N-terminus within the 20S supercomplex (focused refinement on D1/D2 rings, class 1) 手法: EM (単粒子) / 解像度: 3.9 Å 9103 6mdp EMDB-9103, PDB-6mdp: The D1 and D2 domain rings of NSF engaging the SNAP-25 N-terminus within the 20S supercomplex (focused refinement on D1/D2 rings, class 2) 手法: EM (単粒子) / 解像度: 3.8 Å
化合物	ChemComp-ATP: ADENOSINE-5'-TRIPHOSPHATE / ATP / ATP, エネルギー貯蔵分子YM / アデノシン三リン酸 ChemComp-ADP: ADENOSINE-5'-DIPHOSPHATE / ADP / ADP, エネルギー貯蔵分子YM / アデノシン二リン酸
由来	cricetulus griseus (モンゴルキヌゲネズミ) rattus norvegicus (ドブネズミ)
キーワード	HYDROLASE (加水分解酵素) / SNARE / NSF / SNAP / ATPase (ATPアーゼ) / AAA / disassembly (逆アセンブラ) / synapse (シナプス) / membrane fusion / exocytosis (エキソサイトーシス)