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キーワード
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Structure paper

タイトル	Shielding and activation of a viral membrane fusion protein.
ジャーナル・号・ページ	Nat Commun, Vol. 9, Issue 1, Page 349, Year 2018
掲載日	2018年1月24日
著者	Steinar Halldorsson / Sai Li / Mengqiu Li / Karl Harlos / Thomas A Bowden / Juha T Huiskonen /
PubMed 要旨	Entry of enveloped viruses relies on insertion of hydrophobic residues of the viral fusion protein into the host cell membrane. However, the intermediate conformations during fusion remain unknown. ...Entry of enveloped viruses relies on insertion of hydrophobic residues of the viral fusion protein into the host cell membrane. However, the intermediate conformations during fusion remain unknown. Here, we address the fusion mechanism of Rift Valley fever virus. We determine the crystal structure of the Gn glycoprotein and fit it with the Gc fusion protein into cryo-electron microscopy reconstructions of the virion. Our analysis reveals how the Gn shields the hydrophobic fusion loops of the Gc, preventing premature fusion. Electron cryotomography of virions interacting with membranes under acidic conditions reveals how the fusogenic Gc is activated upon removal of the Gn shield. Repositioning of the Gn allows extension of Gc and insertion of fusion loops in the outer leaflet of the target membrane. These data show early structural transitions that enveloped viruses undergo during host cell entry and indicate that analogous shielding mechanisms are utilized across diverse virus families.
リンク	Nat Commun / PubMed:29367607 / PubMed Central
手法	EM (単粒子) / EM (サブトモグラム平均) / X線回折
解像度	1.6 - 20.0 Å
構造データ	4197 6f9b EMDB-4197: Icosahedral reconstruction of Rift Valley fever virus virion PDB-6f9b: Asymmetric unit of Rift Valley fever virus glycoprotein shell 手法: EM (単粒子) / 解像度: 13.3 Å 4198 6f9c EMDB-4198: Localized reconstruction of the Rift Valley fever virus glycoprotein hexamer type 1 PDB-6f9c: Model of the Rift Valley fever virus glycoprotein hexamer type 1 手法: EM (単粒子) / 解像度: 8.0 Å 4199 6f9d EMDB-4199: Localized reconstruction of the Rift Valley fever virus glycoprotein hexamer type 2 PDB-6f9d: Model of the Rift Valley fever virus glycoprotein hexamer type 2 手法: EM (単粒子) / 解像度: 13.3 Å 4200 6f9e EMDB-4200: Localized reconstruction of the Rift Valley fever virus glycoprotein hexamer type 3 PDB-6f9e: Model of the Rift Valley fever virus glycoprotein hexamer type 3 手法: EM (単粒子) / 解像度: 13.3 Å 4201 6f9f EMDB-4201: Localized reconstruction of the Rift Valley fever virus glycoprotein pentamer PDB-6f9f: Model of the Rift Valley fever virus glycoprotein pentamer 手法: EM (単粒子) / 解像度: 13.3 Å EMDB-4202: Subtomogram average of Rift Valley fever virus pentamer at pH 7.5 手法: EM (サブトモグラム平均) / 解像度: 14.0 Å EMDB-4203: Subtomogram average of Rift Valley fever virus hexamer at pH 7.5 手法: EM (サブトモグラム平均) / 解像度: 14.0 Å EMDB-4204: Subtomogram average of Rift Valley fever virus pentamer at pH 7.5 in absence of target membrane 手法: EM (サブトモグラム平均) / 解像度: 17.0 Å EMDB-4205: Subtomogram average of Rift Valley fever virus hexamer at pH 7.5 in absence of target membrane 手法: EM (サブトモグラム平均) / 解像度: 14.0 Å EMDB-4206: Subtomogram average of Rift Valley fever virus pentamer at pH 7.5 in presence of target membrane 手法: EM (サブトモグラム平均) / 解像度: 18.0 Å EMDB-4207: Subtomogram average of Rift Valley fever virus hexamer at pH 7.5 in presence of target membrane 手法: EM (サブトモグラム平均) / 解像度: 20.0 Å EMDB-4208: Subtomogram average of Rift Valley fever virus pentamer at pH 5.0 in absence of target membrane 手法: EM (サブトモグラム平均) / 解像度: 20.0 Å EMDB-4209: Subtomogram average of Rift Valley fever virus hexamer at pH 5.0 in absence of target membrane 手法: EM (サブトモグラム平均) / 解像度: 16.0 Å EMDB-4210: Subtomogram average of Rift Valley fever virus pentamer at pH 5.0 in presence of target membrane 手法: EM (サブトモグラム平均) / 解像度: 20.0 Å EMDB-4211: Subtomogram average of Rift Valley fever virus hexamer at pH 5.0 in presence of target membrane 手法: EM (サブトモグラム平均) / 解像度: 18.0 Å PDB-6f8p: Crystal structure of Gn from Rift Valley fever virus 手法: X-RAY DIFFRACTION / 解像度: 1.6 Å
化合物	ChemComp-HOH: WATER / 水
由来	rift valley fever virus (リフトバレー熱ウイルス)
キーワード	VIRAL PROTEIN (ウイルスタンパク質) / phlebovirus / glycoprotein (糖タンパク質) / bunyavirus (ブニヤウイルス目) / host cell entry / VIRUS (ウイルス) / enveloped virus (エンベロープ (ウイルス)) / RVFV (リフトバレー熱) / fusion protein (融合タンパク質)