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Structure paper

タイトル	The cryo-electron microscopy structure of human transcription factor IIH.
ジャーナル・号・ページ	Nature, Vol. 549, Issue 7672, Page 414-417, Year 2017
掲載日	2017年9月21日
著者	Basil J Greber / Thi Hoang Duong Nguyen / Jie Fang / Pavel V Afonine / Paul D Adams / Eva Nogales /
PubMed 要旨	Human transcription factor IIH (TFIIH) is part of the general transcriptional machinery required by RNA polymerase II for the initiation of eukaryotic gene transcription. Composed of ten subunits ...Human transcription factor IIH (TFIIH) is part of the general transcriptional machinery required by RNA polymerase II for the initiation of eukaryotic gene transcription. Composed of ten subunits that add up to a molecular mass of about 500 kDa, TFIIH is also essential for nucleotide excision repair. The seven-subunit TFIIH core complex formed by XPB, XPD, p62, p52, p44, p34, and p8 is competent for DNA repair, while the CDK-activating kinase subcomplex, which includes the kinase activity of CDK7 as well as the cyclin H and MAT1 subunits, is additionally required for transcription initiation. Mutations in the TFIIH subunits XPB, XPD, and p8 lead to severe premature ageing and cancer propensity in the genetic diseases xeroderma pigmentosum, Cockayne syndrome, and trichothiodystrophy, highlighting the importance of TFIIH for cellular physiology. Here we present the cryo-electron microscopy structure of human TFIIH at 4.4 Å resolution. The structure reveals the molecular architecture of the TFIIH core complex, the detailed structures of its constituent XPB and XPD ATPases, and how the core and kinase subcomplexes of TFIIH are connected. Additionally, our structure provides insight into the conformational dynamics of TFIIH and the regulation of its activity.
リンク	Nature / PubMed:28902838 / PubMed Central
手法	EM (単粒子)
解像度	4.4 - 5.4 Å
構造データ	3802 5of4 EMDB-3802, PDB-5of4: The cryo-EM structure of human TFIIH 手法: EM (単粒子) / 解像度: 4.4 Å EMDB-8816: TFIIH reconstruction from particles sorted for density near the XPD ARCH domain using signal-subtracted classification. 手法: EM (単粒子) / 解像度: 5.4 Å EMDB-8817: TFIIH reconstruction from particles sorted for the XPD FeS-cluster domain using signal-subtracted classification. 手法: EM (単粒子) / 解像度: 4.4 Å
化合物	ChemComp-SF4: IRON/SULFUR CLUSTER / 鉄・硫黄クラスター
由来	homo sapiens (ヒト) Human (ヒト)
キーワード	TRANSCRIPTION (転写 (生物学)) / transcription initiation (転写 (生物学)) / DNA repair (DNA修復) / multiprotein complex (タンパク質複合体) / kinase (キナーゼ) / helicase (ヘリカーゼ)