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Structure paper

タイトル	CryoEM Structure of an Influenza Virus Receptor-Binding Site Antibody-Antigen Interface.
ジャーナル・号・ページ	J Mol Biol, Vol. 429, Issue 12, Page 1829-1839, Year 2017
掲載日	2017年6月16日
著者	Yuhang Liu / Junhua Pan / Simon Jenni / Donald D Raymond / Tim Caradonna / Khoi T Do / Aaron G Schmidt / Stephen C Harrison / Nikolaus Grigorieff /
PubMed 要旨	Structure-based vaccine design depends on extensive structural analyses of antigen-antibody complexes.Single-particle electron cryomicroscopy (cryoEM) can circumvent some of the problems of x-ray ...Structure-based vaccine design depends on extensive structural analyses of antigen-antibody complexes.Single-particle electron cryomicroscopy (cryoEM) can circumvent some of the problems of x-ray crystallography as a pipeline for obtaining the required structures. We have examined the potential of single-particle cryoEM for determining the structure of influenza-virus hemagglutinin (HA):single-chain variable-domain fragment complexes, by studying a complex we failed to crystallize in pursuing an extended project on the human immune response to influenza vaccines.The result shows that a combination of cryoEM and molecular modeling can yield details of the antigen-antibody interface, although small variation in the twist of the rod-likeHA trimer limited the overall resolution to about 4.5Å.Comparison of principal 3D classes suggests ways to modify the HA trimer to overcome this limitation. A closely related antibody from the same donor did yield crystals when bound with the same HA, giving us an independent validation of the cryoEM results.The two structures also augment our understanding of receptor-binding site recognition by antibodies that neutralize a wide range of influenza-virus variants.
リンク	J Mol Biol / PubMed:28506635 / PubMed Central
手法	EM (単粒子) / X線回折
解像度	3.4 - 4.8 Å
構造データ	8561 5ujz EMDB-8561, PDB-5ujz: CryoEM structure of an influenza virus receptor-binding site antibody-antigen interface - Class 1 手法: EM (単粒子) / 解像度: 4.8 Å 8562 5uk0 EMDB-8562, PDB-5uk0: CryoEM structure of an influenza virus receptor-binding site antibody-antigen interface - Class 2 手法: EM (単粒子) / 解像度: 4.8 Å 8563 5uk1 EMDB-8563, PDB-5uk1: CryoEM structure of an influenza virus receptor-binding site antibody-antigen interface - Class 3 手法: EM (単粒子) / 解像度: 4.8 Å 8564 5uk2 EMDB-8564, PDB-5uk2: CryoEM structure of an influenza virus receptor-binding site antibody-antigen interface - Class 4 手法: EM (単粒子) / 解像度: 4.8 Å PDB-5ug0: Human antibody H2897 in complex with influenza hemagglutinin H1 Solomon Islands/03/2006 手法: X-RAY DIFFRACTION / 解像度: 3.4 Å
化合物	ChemComp-NAG: 2-acetamido-2-deoxy-beta-D-glucopyranose / N-アセチル-β-D-グルコサミン / N-アセチルグルコサミン
由来	influenza a virus (a/solomon islands/3/2006(h1n1)) (A型インフルエンザウイルス) influenza a virus (A型インフルエンザウイルス) homo sapiens (ヒト)
キーワード	VIRAL PROTEIN/IMMUNE SYSTEM (ウイルス性) / Influenza HA / antibody (抗体) / complex / VIRAL PROTEIN-IMMUNE SYSTEM complex (ウイルス性) / Viral glycoprotein / Hemagglutinin (ヘマグルチニン) / Antibody fragment