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TitleTranscription termination factor ρ polymerizes under stress.
Journal, issue, pagesbioRxiv, Year 2023
Publish dateAug 18, 2023
AuthorsBing Wang / Nelly Said / Tarek Hilal / Mark Finazzo / Markus C Wahl / Irina Artsimovitch /
PubMed AbstractBacterial RNA helicase ρ is a genome sentinel that terminates synthesis of damaged and junk RNAs that are not translated by the ribosome. Co-transcriptional RNA surveillance by ρ is essential for ...Bacterial RNA helicase ρ is a genome sentinel that terminates synthesis of damaged and junk RNAs that are not translated by the ribosome. Co-transcriptional RNA surveillance by ρ is essential for quality control of the transcriptome during optimal growth. However, it is unclear how bacteria protect their RNAs from overzealous ρ during dormancy or stress, conditions common in natural habitats. Here we used cryogenic electron microscopy, biochemical, and genetic approaches to show that residue substitutions, ADP, or ppGpp promote hyper-oligomerization of ρ. Our results demonstrate that nucleotides bound at subunit interfaces control ρ switching from active hexamers to inactive higher-order oligomers and extended filaments. Polymers formed upon exposure to antibiotics or ppGpp disassemble when stress is relieved, thereby directly linking termination activity to cellular physiology. Inactivation of ρ through hyper-oligomerization is a regulatory strategy shared by RNA polymerases, ribosomes, and metabolic enzymes across all life.
External linksbioRxiv / PubMed:37645988 / PubMed Central
MethodsEM (single particle) / EM (helical sym.)
Resolution2.63 - 3.5 Å
Structure data

18130

8q3n

EMDB-18130, PDB-8q3n:
Bacterial transcription termination factor Rho + ADP
Method: EM (single particle) / Resolution: 2.63 Å

18131

8q3o

EMDB-18131, PDB-8q3o:
Bacterial transcription termination factor Rho + pppGpp
Method: EM (single particle) / Resolution: 3.0 Å

18132

8q3p

EMDB-18132, PDB-8q3p:
Bacterial transcription termination factor Rho G150D mutant
Method: EM (helical sym.) / Resolution: 3.5 Å

18133

8q3q

EMDB-18133, PDB-8q3q:
Bacterial transcription termination factor Rho G152D mutant
Method: EM (helical sym.) / Resolution: 3.3 Å

Chemicals

ChemComp-ADP:
ADENOSINE-5'-DIPHOSPHATE / ADP, energy-carrying molecule*YM

ChemComp-MG:
Unknown entry

ChemComp-0O2:
guanosine 5'-(tetrahydrogen triphosphate) 3'-(trihydrogen diphosphate)

Source
  • escherichia coli (E. coli)
  • escherichia (bacteria)
KeywordsTRANSCRIPTION / Rho / termination

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