Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	Molecular mechanism of dynein-dynactin complex assembly by LIS1.
Journal, issue, pages	Science, Vol. 383, Issue 6690, Page eadk8544, Year 2024
Publish date	Mar 29, 2024
Authors	Kashish Singh / Clinton K Lau / Giulia Manigrasso / José B Gama / Reto Gassmann / Andrew P Carter /
PubMed Abstract	Cytoplasmic dynein is a microtubule motor vital for cellular organization and division. It functions as a ~4-megadalton complex containing its cofactor dynactin and a cargo-specific coiled-coil ...Cytoplasmic dynein is a microtubule motor vital for cellular organization and division. It functions as a ~4-megadalton complex containing its cofactor dynactin and a cargo-specific coiled-coil adaptor. However, how dynein and dynactin recognize diverse adaptors, how they interact with each other during complex formation, and the role of critical regulators such as lissencephaly-1 (LIS1) protein (LIS1) remain unclear. In this study, we determined the cryo-electron microscopy structure of dynein-dynactin on microtubules with LIS1 and the lysosomal adaptor JIP3. This structure reveals the molecular basis of interactions occurring during dynein activation. We show how JIP3 activates dynein despite its atypical architecture. Unexpectedly, LIS1 binds dynactin's p150 subunit, tethering it along the length of dynein. Our data suggest that LIS1 and p150 constrain dynein-dynactin to ensure efficient complex formation.
External links	Science / PubMed:38547289 / PubMed Central
Methods	EM (single particle)
Resolution	3.5 - 10.0 Å
Structure data	17825 8pqv EMDB-17825, PDB-8pqv: Cytoplasmic dynein-1 motor domain in post-powerstroke state Method: EM (single particle) / Resolution: 4.0 Å 17826 8pqw EMDB-17826, PDB-8pqw: Cytoplasmic dynein-1 motor domain bound to dynactin-p150glued and LIS1 Method: EM (single particle) / Resolution: 4.2 Å 17828 8pqy EMDB-17828, PDB-8pqy: Cytoplasmic dynein-1 motor domain bound to LIS1 Method: EM (single particle) / Resolution: 3.8 Å 17829 8pqz EMDB-17829, PDB-8pqz: Cytoplasmic dynein-1 A1/A2 motor domains bound to LIS1 Method: EM (single particle) / Resolution: 5.5 Å 17830 8pr0 EMDB-17830, PDB-8pr0: Cytoplasmic dynein-A heavy chain bound to dynactin-p150glued and IC-LC tower Method: EM (single particle) / Resolution: 9.4 Å 17831 8pr1 EMDB-17831, PDB-8pr1: Cytoplasmic dynein-B heavy chain bound to IC-LC tower Method: EM (single particle) / Resolution: 8.2 Å 17832 8pr2 EMDB-17832, PDB-8pr2: Cytoplasmic dynein-1 heavy chain bound to JIP3-LZI Method: EM (single particle) / Resolution: 3.8 Å 17833 8pr3 EMDB-17833, PDB-8pr3: Cytoplasmic dynein-1 heavy chain bound to JIP3-RH1 Method: EM (single particle) / Resolution: 3.9 Å 17834 8pr4 EMDB-17834, PDB-8pr4: Dynactin pointed end bound to JIP3 Method: EM (single particle) / Resolution: 3.5 Å EMDB-17835: Consensus cryo-EM structure of Dynein-Dynactin-JIP3(1-185)-LIS1 Method: EM (single particle) / Resolution: 4.5 Å EMDB-17836: Consensus cryo-EM structure of Dynein-dynactin-JIP3(1-560)-LIS1 Method: EM (single particle) / Resolution: 4.9 Å 17873 8ptk EMDB-17873, PDB-8ptk: Composite structure of Dynein-Dynactin-JIP3-LIS1 Method: EM (single particle) / Resolution: 10.0 Å PDB-8pr5: Structure of the autoinhibited dynactin p150glued projection Method: ELECTRON MICROSCOPY / Resolution: 8.6 Å
Chemicals	ChemComp-ADP: ADENOSINE-5'-DIPHOSPHATE / ADP, energy-carrying moleculeYM ChemComp-MG: Unknown entry ChemComp-ATP: ADENOSINE-5'-TRIPHOSPHATE / ATP, energy-carrying moleculeYM ChemComp-ZN: Unknown entry ChemComp-ANP: PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER / AMP-PNP, energy-carrying molecule analogue*YM
Source	homo sapiens (human) sus scrofa (pig)
Keywords	MOTOR PROTEIN / Dynein / AAA-Atpase / p150 / LIS1 / dynactin / LC8 / TCTEX1 / JIP3