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| Title | Nearly complete structure of bacteriophage DT57C reveals architecture of head-to-tail interface and lateral tail fibers. |
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| Journal, issue, pages | Nat Commun, Vol. 14, Issue 1, Page 8205, Year 2023 |
| Publish date | Dec 11, 2023 |
 Authors | Rafael Ayala / Andrey V Moiseenko / Ting-Hua Chen / Eugene E Kulikov / Alla K Golomidova / Philipp S Orekhov / Maya A Street / Olga S Sokolova / Andrey V Letarov / Matthias Wolf /     |
| PubMed Abstract | The T5 family of viruses are tailed bacteriophages characterized by a long non-contractile tail. The bacteriophage DT57C is closely related to the paradigmal T5 phage, though it recognizes a ...The T5 family of viruses are tailed bacteriophages characterized by a long non-contractile tail. The bacteriophage DT57C is closely related to the paradigmal T5 phage, though it recognizes a different receptor (BtuB) and features highly divergent lateral tail fibers (LTF). Considerable portions of T5-like phages remain structurally uncharacterized. Here, we present the structure of DT57C determined by cryo-EM, and an atomic model of the virus, which was further explored using all-atom molecular dynamics simulations. The structure revealed a unique way of LTF attachment assisted by a dodecameric collar protein LtfC, and an unusual composition of the phage neck constructed of three protein rings. The tape measure protein (TMP) is organized within the tail tube in a three-stranded parallel α-helical coiled coil which makes direct contact with the genomic DNA. The presence of the C-terminal fragment of the TMP that remains within the tail tip suggests that the tail tip complex returns to its original state after DNA ejection. Our results provide a complete atomic structure of a T5-like phage, provide insights into the process of DNA ejection as well as a structural basis for the design of engineered phages and future mechanistic studies. |
 External links | Nat Commun / PubMed:38081816 / PubMed Central |
| Methods | EM (single particle) / EM (helical sym.) / EM (tomography) |
| Resolution | 2.9 - 7.9 Å |
| Structure data | EMDB-34920, PDB-8ho3: EMDB-34949, PDB-8hqk: EMDB-34952, PDB-8hqo: EMDB-34955, PDB-8hqz: EMDB-34968, PDB-8hre: EMDB-34972, PDB-8hrg:  EMDB-34973: Curved tail segment of the DT57C bacteriophage in the full state  EMDB-35003: Focused refinement of the TTrP region of the neck of the DT57C bacteriophage in the full state  EMDB-35006: Map of the neck of the DT57C bacteriophage in the full state with C12 symmetry  EMDB-35099: Consensus refinement for the baseplate of DT57C bacteriophage  EMDB-35100: Focused refinement of the baseplate of DT57C focused around its core region  EMDB-35101: Focused refinement of the baseplate of DT57C focused around the baseplate hub protein  EMDB-37449: Focused map for the TMP N-terminus of DT57C  EMDB-37518: Tomogram of DT57C bacteriophage (1 of 9)  EMDB-37519: Tomogram of DT57C bacteriophage (2 out of 9)  EMDB-37521: Tomogram of DT57C bacteriophage (3 out of 9)  EMDB-37531: Tomogram of DT57C bacteriophage (4 out of 9)  EMDB-37534: Tomogram of DT57C bacteriophage (5 out of 9)  EMDB-37536: Tomogram of DT57C bacteriophage (6 out of 9)  EMDB-37539: Tomogram of DT57C bacteriophage (7 out of 9)  EMDB-37543: Tomogram of DT57C bacteriophage (8 out of 9)  EMDB-37544: Tomogram of DT57C bacteriophage (9 out of 9) |
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 Keywords | VIRUS / Capsid / T5 / MHP / VIRAL PROTEIN / Neck / Portal / Baseplate / Straight fiber / Tail tube |
escherichia phage dt57c (virus)