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Structure paper

TitleAsymmetric Molecular Architecture of the Human γ-Tubulin Ring Complex.
Journal, issue, pagesCell, Vol. 180, Issue 1, Page 165-175.e16, Year 2020
Publish dateJan 9, 2020
AuthorsMichal Wieczorek / Linas Urnavicius / Shih-Chieh Ti / Kelly R Molloy / Brian T Chait / Tarun M Kapoor /
PubMed AbstractThe γ-tubulin ring complex (γ-TuRC) is an essential regulator of centrosomal and acentrosomal microtubule formation, yet its structure is not known. Here, we present a cryo-EM reconstruction of the ...The γ-tubulin ring complex (γ-TuRC) is an essential regulator of centrosomal and acentrosomal microtubule formation, yet its structure is not known. Here, we present a cryo-EM reconstruction of the native human γ-TuRC at ∼3.8 Å resolution, revealing an asymmetric, cone-shaped structure. Pseudo-atomic models indicate that GCP4, GCP5, and GCP6 form distinct Y-shaped assemblies that structurally mimic GCP2/GCP3 subcomplexes distal to the γ-TuRC "seam." We also identify an unanticipated structural bridge that includes an actin-like protein and spans the γ-TuRC lumen. Despite its asymmetric architecture, the γ-TuRC arranges γ-tubulins into a helical geometry poised to nucleate microtubules. Diversity in the γ-TuRC subunits introduces large (>100,000 Å) surfaces in the complex that allow for interactions with different regulatory factors. The observed compositional complexity of the γ-TuRC could self-regulate its assembly into a cone-shaped structure to control microtubule formation across diverse contexts, e.g., within biological condensates or alongside existing filaments.
External linksCell / PubMed:31862189 / PubMed Central
MethodsEM (single particle)
Resolution3.4 - 6.6 Å
Structure data

21054

6v5v

EMDB-21054, PDB-6v5v:
Structure of gamma-tubulin in the native human gamma-tubulin ring complex
Method: EM (single particle) / Resolution: 3.8 Å

21060

6v69

EMDB-21060, PDB-6v69:
Structures of GCP4 and GCP5 in the native human gamma-tubulin ring complex
Method: EM (single particle) / Resolution: 4.2 Å

EMDB-21063:
Density map surrounding actin-like protein in reconstruction of the native gamma-tubulin ring complex
Method: EM (single particle) / Resolution: 4.1 Å

21067

6v6b

EMDB-21067, PDB-6v6b:
Structures of GCP2 and GCP3 in the native human gamma-tubulin ring complex
Method: EM (single particle) / Resolution: 3.8 Å

21068

6v6c

EMDB-21068, PDB-6v6c:
Structure of GCP6 in the native human gamma-tubulin ring complex
Method: EM (single particle) / Resolution: 4.5 Å

EMDB-21069:
Desnity map of lumenal bridge in reconstruction of the native human gamma-tubulin ring complex
Method: EM (single particle) / Resolution: 3.4 Å

EMDB-21070:
Density map for overlap region in reconstruction of native human gamma-tubulin ring complex
Method: EM (single particle) / Resolution: 6.6 Å

21073

6v6s

EMDB-21073, PDB-6v6s:
Structure of the native human gamma-tubulin ring complex
Method: EM (single particle) / Resolution: 4.3 Å

EMDB-21074:
Overall density map of the native human gamma-tubulin ring complex
Method: EM (single particle) / Resolution: 3.8 Å

Chemicals

ChemComp-GDP:
GUANOSINE-5'-DIPHOSPHATE / GDP, energy-carrying molecule*YM

ChemComp-ADP:
ADENOSINE-5'-DIPHOSPHATE / ADP, energy-carrying molecule*YM

Source
  • homo sapiens (human)
KeywordsSTRUCTURAL PROTEIN / Tubulin / gamma-tubulin / gamma-tubulin ring complex / gTuRC / g-TuRC / microtubule / microtubule nucleation / single particle cryo-EM structure / GCP / GCP4 / GCP5 / GCP2 / GCP3 / gamma-TuSC / gTuSC / GCP6

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