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-Structure paper
Title | Cryo-EM structures of RNA polymerase II-nucleosome complexes rewrapping transcribed DNA. |
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Journal, issue, pages | J Biol Chem, Vol. 299, Issue 12, Page 105477, Year 2023 |
Publish date | Nov 17, 2023 |
Authors | Munetaka Akatsu / Haruhiko Ehara / Tomoya Kujirai / Risa Fujita / Tomoko Ito / Ken Osumi / Mitsuo Ogasawara / Yoshimasa Takizawa / Shun-Ichi Sekine / Hitoshi Kurumizaka / |
PubMed Abstract | RNA polymerase II (RNAPII) transcribes DNA wrapped in the nucleosome by stepwise pausing, especially at nucleosomal superhelical locations -5 and -1 [SHL(-5) and SHL(-1), respectively]. In the ...RNA polymerase II (RNAPII) transcribes DNA wrapped in the nucleosome by stepwise pausing, especially at nucleosomal superhelical locations -5 and -1 [SHL(-5) and SHL(-1), respectively]. In the present study, we performed cryo-electron microscopy analyses of RNAPII-nucleosome complexes paused at a major nucleosomal pausing site, SHL(-1). We determined two previously undetected structures, in which the transcribed DNA behind RNAPII is sharply kinked at the RNAPII exit tunnel and rewrapped around the nucleosomal histones in front of RNAPII by DNA looping. This DNA kink shifts the DNA orientation toward the nucleosome, and the transcribed DNA region interacts with basic amino acid residues of histones H2A, H2B, and H3 exposed by the RNAPII-mediated nucleosomal DNA peeling. The DNA loop structure was not observed in the presence of the transcription elongation factors Spt4/5 and Elf1. These RNAPII-nucleosome structures provide important information for understanding the functional relevance of DNA looping during transcription elongation in the nucleosome. |
External links | J Biol Chem / PubMed:37981206 / PubMed Central |
Methods | EM (single particle) |
Resolution | 3.2 - 6.7 Å |
Structure data | EMDB-36251, PDB-8jh2: EMDB-36252, PDB-8jh3: EMDB-36253, PDB-8jh4: EMDB-37848: RNA polymerase II elongation complex bound with Elf1, Spt4/5 and foreign DNA, stalled at SHL(0) of the nucleosome |
Chemicals | ChemComp-ZN: ChemComp-MG: |
Source |
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Keywords | Transcription/DNA/RNA / Transcription-DNA-RNA complex / RNAPII / Cryo-EM |