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TitleA bipolar spindle of antiparallel ParM filaments drives bacterial plasmid segregation.
Journal, issue, pagesScience, Vol. 338, Issue 6112, Page 1334-1337, Year 2012
Publish dateDec 7, 2012
AuthorsP Gayathri / T Fujii / J Møller-Jensen / F van den Ent / K Namba / J Löwe /
PubMed AbstractTo ensure their stable inheritance by daughter cells during cell division, bacterial low-copy-number plasmids make simple DNA segregating machines that use an elongating protein filament between ...To ensure their stable inheritance by daughter cells during cell division, bacterial low-copy-number plasmids make simple DNA segregating machines that use an elongating protein filament between sister plasmids. In the ParMRC system of the Escherichia coli R1 plasmid, ParM, an actinlike protein, forms the spindle between ParRC complexes on sister plasmids. By using a combination of structural work and total internal reflection fluorescence microscopy, we show that ParRC bound and could accelerate growth at only one end of polar ParM filaments, mechanistically resembling eukaryotic formins. The architecture of ParM filaments enabled two ParRC-bound filaments to associate in an antiparallel orientation, forming a bipolar spindle. The spindle elongated as a bundle of at least two antiparallel filaments, thereby pushing two plasmid clusters toward the poles.
External linksScience / PubMed:23112295 / PubMed Central
MethodsEM (helical sym.) / X-ray diffraction / EM (single particle)
Resolution2 - 7.2 Å
Structure data

1980

4a6j

EMDB-1980: CryoEM map of ParM filament at 7.2 Angstrom resolution
PDB-4a6j: Structural model of ParM filament based on CryoEM map
Method: EM (helical sym.) / Resolution: 7.2 Å

PDB-4a61:
ParM from plasmid R1 in complex with AMPPNP
Method: X-RAY DIFFRACTION / Resolution: 2 Å

PDB-4a62:
ParM from R1 plasmid in complex with peptide from C-terminus of ParR
Method: X-RAY DIFFRACTION / Resolution: 2.2 Å

Chemicals

ChemComp-ANP:
PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER / AMP-PNP, energy-carrying molecule analogue*YM

ChemComp-MG:
Unknown entry

ChemComp-HOH:
WATER

Source
  • escherichia coli (E. coli)
KeywordsTRANSPORT PROTEIN / PLASMID SEGREGATION / ACTIN-FOLD

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