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Structure paper

TitleSlyB encapsulates outer membrane proteins in stress-induced lipid nanodomains
Journal, issue, pagesNature, Year 2023
Publish dateMay 14, 2021 (structure data deposition date)
AuthorsJanssens A / Nguyen VS / Cecil AJ / Van der Verren SE / Timmerman E / Deghelt M / Pak AJ / Collet JF / Impens F / Remaut H
External linksNature / Search PubMed
MethodsEM (single particle)
Resolution3.4 - 6.7 Å
Structure data

EMDB-12945: Cryo-EM reconstruction of decameric SlyB from Escherichia coli K12
Method: EM (single particle) / Resolution: 5.6 Å

EMDB-12946: Cryo-EM reconstruction of dodecameric SlyB from Escherichia coli K12
Method: EM (single particle) / Resolution: 4.3 Å

EMDB-12947: Cryo-EM structure of tridecameric SlyB from Escherichia coli K12
Method: EM (single particle) / Resolution: 5.8 Å

EMDB-12948: Cryo-EM reconstruction of SlyB14-BamA from Escherichia coli
Method: EM (single particle) / Resolution: 6.7 Å

12950

7ojg

EMDB-12950: Cryo-EM structure of undecameric SlyB from Escherichia coli K12
PDB-7ojg: CRYO-EM STRUCTURE OF UNDECAMERIC SLYB FROM ESCHERICHIA COLI K12
Method: EM (single particle) / Resolution: 3.4 Å

PDB-7ojf:
CRYO-EM STRUCTURE OF SLYB13-BAMA FROM ESCHERICHIA COLI
Method: ELECTRON MICROSCOPY / Resolution: 3.9 Å

Chemicals

ChemComp-PLM:
PALMITIC ACID

ChemComp-L8Z:
(2~{R},4~{R},5~{R},6~{R})-6-[(1~{R})-1,2-bis(oxidanyl)ethyl]-2-[[(2~{R},3~{S},4~{R},5~{R},6~{R})-5-[[(~{E},3~{R})-3-dodecanoyloxytetradec-5-enoyl]amino]-6-[[(2~{R},3~{S},4~{R},5~{R},6~{R})-3-oxidanyl-5-[[(~{E},3~{R})-3-oxidanyltetradec-11-enoyl]amino]-4-[(~{E},3~{R})-3-oxidanyltetradec-5-enoyl]oxy-6-phosphonooxy-oxan-2-yl]methoxy]-3-phosphonooxy-4-[(~{E},3~{R})-3-tetradecanoyloxytetradec-7-enoyl]oxy-oxan-2-yl]methoxy]-4,5-bis(oxidanyl)oxane-2-carboxylic acid

ChemComp-LPP:
2-(HEXADECANOYLOXY)-1-[(PHOSPHONOOXY)METHYL]ETHYL HEXADECANOATE / phospholipid*YM

ChemComp-GOL:
GLYCEROL

Source
  • Escherichia coli (E. coli)
  • escherichia coli bw25113 (bacteria)
KeywordsMEMBRANE PROTEIN / OUTER MEMBRANE CHAPERON / 2TM GLYCINE ZIPPER / OUTER MEMBRANE LIPOPROTEIN SLYB / LPS-LP BINDING PROTEIN / OUTER MEMBRANE CHAPERONE

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