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-Structure paper
Title | Structure and mechanism of the Nap adhesion complex from the human pathogen Mycoplasma genitalium. |
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Journal, issue, pages | Nat Commun, Vol. 11, Issue 1, Page 2877, Year 2020 |
Publish date | Jun 8, 2020 |
Authors | David Aparicio / Margot P Scheffer / Marina Marcos-Silva / David Vizarraga / Lasse Sprankel / Mercè Ratera / Miriam S Weber / Anja Seybert / Sergi Torres-Puig / Luis Gonzalez-Gonzalez / Julian Reitz / Enrique Querol / Jaume Piñol / Oscar Q Pich / Ignacio Fita / Achilleas S Frangakis / |
PubMed Abstract | Mycoplasma genitalium is a human pathogen adhering to host target epithelial cells and causing urethritis, cervicitis and pelvic inflammatory disease. Essential for infectivity is a transmembrane ...Mycoplasma genitalium is a human pathogen adhering to host target epithelial cells and causing urethritis, cervicitis and pelvic inflammatory disease. Essential for infectivity is a transmembrane adhesion complex called Nap comprising proteins P110 and P140. Here we report the crystal structure of P140 both alone and in complex with the N-terminal domain of P110. By cryo-electron microscopy (cryo-EM) and tomography (cryo-ET) we find closed and open Nap conformations, determined at 9.8 and 15 Å, respectively. Both crystal structures and the cryo-EM structure are found in a closed conformation, where the sialic acid binding site in P110 is occluded. By contrast, the cryo-ET structure shows an open conformation, where the binding site is accessible. Structural information, in combination with functional studies, suggests a mechanism for attachment and release of M. genitalium to and from the host cell receptor, in which Nap conformations alternate to sustain motility and guarantee infectivity. |
External links | Nat Commun / PubMed:32513917 / PubMed Central |
Methods | EM (subtomogram averaging) / EM (single particle) / X-ray diffraction |
Resolution | 2.65 - 15.0 Å |
Structure data | EMDB-10259: EMDB-10260: EMDB-10890: cryo-electron density map of the P140-P110 heterodimer PDB-6rut: PDB-6s3u: |
Chemicals | ChemComp-HOH: |
Source |
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Keywords | SUGAR BINDING PROTEIN / Adhesion complex / PROTEIN BINDING / CELL ADHESION / P110/P140 Nap Mycoplasma genitalium surface adhesin |