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TitleStructure of Aspergillus fumigatus Cytosolic Thiolase: Trapped Tetrahedral Reaction Intermediates and Activation by Monovalent Cations
Journal, issue, pagesAcs Catalysis, Vol. 8(3), Page 1973-1989, Year 2018
Publish dateAug 21, 2017 (structure data deposition date)
AuthorsMarshall, A.C. / Bond, C.S. / Bruning, J.B.
External linksAcs Catalysis / Search PubMed
MethodsX-ray diffraction
Resolution1.702 - 2.25 Å
Structure data

PDB-6aqp:
Aspergillus fumigatus Cytosolic Thiolase: Acetylated enzyme in complex with CoA and potassium ions
Method: X-RAY DIFFRACTION / Resolution: 1.8 Å

PDB-6are:
Aspergillus fumigatus Cytosolic Thiolase in complex with two tetrahedral reaction intermediates and ammonium ions
Method: X-RAY DIFFRACTION / Resolution: 1.75 Å

PDB-6arf:
Aspergillus fumigatus Cytosolic Thiolase: Apo enzyme in complex with potassium ions
Method: X-RAY DIFFRACTION / Resolution: 1.702 Å

PDB-6arg:
Aspergillus fumigatus Cytosolic Thiolase: Apo enzyme in complex with rubidium ions
Method: X-RAY DIFFRACTION / Resolution: 1.782 Å

PDB-6arl:
Aspergillus fumigatus Cytosolic Thiolase: Apo enzyme in complex with rubidium ions
Method: X-RAY DIFFRACTION / Resolution: 1.9 Å

PDB-6arr:
Aspergillus fumigatus Cytosolic Thiolase: Apo enzyme in complex with cesium ions
Method: X-RAY DIFFRACTION / Resolution: 1.822 Å

PDB-6art:
Aspergillus fumigatus Cytosolic Thiolase: Apo enzyme in complex with cesium ions
Method: X-RAY DIFFRACTION / Resolution: 2.25 Å

Chemicals

ChemComp-COA:
COENZYME A

ChemComp-K:
Unknown entry

ChemComp-CL:
Unknown entry

ChemComp-ACO:
ACETYL COENZYME *A

ChemComp-HOH:
WATER

ChemComp-NH4:
AMMONIUM ION

ChemComp-ACT:
ACETATE ION

ChemComp-GOL:
GLYCEROL

ChemComp-RB:
RUBIDIUM ION

ChemComp-CS:
Unknown entry

Source
  • neosartorya fumigata (strain atcc mya-4609 / af293 / cbs 101355 / fgsc a1100) (mold)
  • aspergillus fumigatus af293 (mold)
KeywordsTRANSFERASE / thiolase / Claisen condensation / reaction intermediate / monovalent cation binding

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