EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	CryoEM Structures of Native Quinol-Dependent Nitric Oxide Reductase in Resting and Quinol-Bound States.
ジャーナル・号・ページ	ACS Bio Med Chem Au, Vol. 6, Issue 2, Page 145-159, Year 2026
掲載日	2026年4月15日
著者	Faisal T Khaja / Allegra Mboukou / Louie P Aspinall / Charlotte E Hawksworth / Robert R Eady / Svetlana V Antonyuk / Stephen P Muench / S Samar Hasnain /
PubMed 要旨	The membrane-bound quinol-dependent nitric oxide reductases (qNORs), which are members of the respiratory heme-copper oxidase superfamily, are of major importance to food production, environment, and ...The membrane-bound quinol-dependent nitric oxide reductases (qNORs), which are members of the respiratory heme-copper oxidase superfamily, are of major importance to food production, environment, and human health. They are unique to bacteria and catalyze N-N bond formation, converting nitric oxide (NO) to generate the enzymatic product, nitrous oxide (NO), in agricultural and pathogenic conditions. High-resolution qNOR structures have been reported from two bacterial species, in which the molecular size of the protein was increased by the insertion of apocytochrome b (BRIL) at the C-terminus to facilitate cryoEM structure determination. However, it remains uncertain how BRIL fusion alters the native structure of these metalloenzymes. Here, we present the first high-resolution structure of qNOR (qNOR) determined without a fusion tag at two different pH values, revealing structural differences near the catalytic core as well as overall conformational changes between the native and fusion-tagged structures. The native enzyme shows a bell-shaped pH dependence of enzymatic activity, like nitrite reductase, the preceding enzyme in the denitrification pathway, which generates the substrate NO. In addition, we report structures of qNOR bound to quinol and hydroxyquinol that provide valuable insight into the potential electron transfer pathway originating from Trp718 to the redox centers.
リンク	ACS Bio Med Chem Au / PubMed:42006251 / PubMed Central
手法	EM (単粒子)
解像度	2.3 - 3.4 Å
構造データ	55213 9st9 EMDB-55213, PDB-9st9: CryoEM structure of native quinol dependent Nitric Oxide Reductase at pH 6.5 手法: EM (単粒子) / 解像度: 3.1 Å 55214 9sta EMDB-55214, PDB-9sta: CryoEM structure of native quinol dependent Nitric Oxide Reductase with HQE at pH 6.5 手法: EM (単粒子) / 解像度: 2.4 Å 56718 28pn EMDB-56718, PDB-28pn: CryoEM structure of native quinol dependent Nitric Oxide Reductase at pH 8.0 on gold grid. 手法: EM (単粒子) / 解像度: 2.7 Å 56720 28pp EMDB-56720, PDB-28pp: CryoEM structure of native quinol dependent Nitric Oxide Reductase Arg720Ala variant at pH 6.5 on gold grid. 手法: EM (単粒子) / 解像度: 2.9 Å 56721 28pq EMDB-56721, PDB-28pq: CryoEM structure of native quinol dependent Nitric Oxide Reductase Trp718Ala variant at pH 6.5 on gold grid. 手法: EM (単粒子) / 解像度: 2.4 Å 56722 28pr EMDB-56722, PDB-28pr: CryoEM structure of native quinol dependent Nitric Oxide Reductase Trp718Ala variant with quino at pH 6.5 on gold grid. 手法: EM (単粒子) / 解像度: 2.4 Å 66367 9wyk EMDB-66367, PDB-9wyk: CryoEM structure of native quinol dependent Nitric Oxide Reductase at pH 8.0. 手法: EM (単粒子) / 解像度: 2.65 Å 66368 9wyl EMDB-66368, PDB-9wyl: CryoEM structure of quinol dependent Nitric Oxide Reductase with BRIL 手法: EM (単粒子) / 解像度: 3.4 Å 66369 9wym EMDB-66369, PDB-9wym: CryoEM structure of native quinol dependent Nitric Oxide Reductase with HQN at pH 6.5 手法: EM (単粒子) / 解像度: 2.3 Å
化合物	ChemComp-HEM: PROTOPORPHYRIN IX CONTAINING FE ChemComp-CA: Unknown entry ChemComp-FE: Unknown entry ChemComp-HOH: WATER ChemComp-LMT: DODECYL-BETA-D-MALTOSIDE / 可溶化剤YM ChemComp-UQ5: 2,3-DIMETHOXY-5-METHYL-6-(3,11,15,19-TETRAMETHYL-EICOSA-2,6,10,14,18-PENTAENYL)-[1,4]BENZOQUINONE ChemComp-HQE: benzene-1,4-diol ChemComp-CU: COPPER (II) ION ChemComp-HQN*: benzene-1,2,4-triol
由来	achromobacter xylosoxidans (バクテリア)
キーワード	MEMBRANE PROTEIN / quinol-dependent Nitric Oxide Reductase