Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	Coupling of ribosome biogenesis and translation initiation in human mitochondria.
Journal, issue, pages	Nat Commun, Vol. 16, Issue 1, Page 3641, Year 2025
Publish date	Apr 17, 2025
Authors	Marleen Heinrichs / Anna Franziska Finke / Shintaro Aibara / Angelique Krempler / Angela Boshnakovska / Peter Rehling / Hauke S Hillen / Ricarda Richter-Dennerlein /
PubMed Abstract	Biogenesis of mitoribosomes requires dedicated chaperones, RNA-modifying enzymes, and GTPases, and defects in mitoribosome assembly lead to severe mitochondriopathies in humans. Here, we characterize ...Biogenesis of mitoribosomes requires dedicated chaperones, RNA-modifying enzymes, and GTPases, and defects in mitoribosome assembly lead to severe mitochondriopathies in humans. Here, we characterize late-step assembly states of the small mitoribosomal subunit (mtSSU) by combining genetic perturbation and mutagenesis analysis with biochemical and structural approaches. Isolation of native mtSSU biogenesis intermediates via a FLAG-tagged variant of the GTPase MTG3 reveals three distinct assembly states, which show how factors cooperate to mature the 12S rRNA. In addition, we observe four distinct primed initiation mtSSU states with an incompletely matured rRNA, suggesting that biogenesis and translation initiation are not mutually exclusive processes but can occur simultaneously. Together, these results provide insights into mtSSU biogenesis and suggest a functional coupling between ribosome biogenesis and translation initiation in human mitochondria.
External links	Nat Commun / PubMed:40240327 / PubMed Central
Methods	EM (single particle)
Resolution	3.0 - 3.8 Å
Structure data	EMDB-51039: Translation-initiation state of human mitochondrial ribosome small subunit (State G) Method: EM (single particle) / Resolution: 3.1 Å EMDB-51042: Translation-initiation state of human mitochondrial ribosome small subunit (State G) Method: EM (single particle) / Resolution: 3.1 Å EMDB-51043: Translation-initiation state of human mitochondrial ribosome small subunit (State E) Method: EM (single particle) / Resolution: 3.6 Å EMDB-51045: Translation-initiation state of human mitochondrial ribosome small subunit (State E) Method: EM (single particle) / Resolution: 3.3 Å EMDB-51046: Translation-initiation state of human mitochondrial ribosome small subunit (State D) Method: EM (single particle) / Resolution: 3.1 Å EMDB-51047: Translation-initiation state of human mitochondrial ribosome small subunit (State D) Method: EM (single particle) / Resolution: 3.0 Å EMDB-51048: Translation-initiation state of human mitochondrial ribosome small subunit (State F) Method: EM (single particle) / Resolution: 3.8 Å EMDB-51049: Translation-initiation state of human mitochondrial ribosome small subunit (State F) Method: EM (single particle) / Resolution: 3.6 Å EMDB-51050: Translation-initiation state of human mitochondrial ribosome small subunit (State F) Method: EM (single particle) / Resolution: 3.3 Å EMDB-51051: Assembly intermediate of human mitochondrial ribosome small subunit (State C) Method: EM (single particle) / Resolution: 3.1 Å EMDB-51052: Assembly intermediate of human mitochondrial ribosome small subunit (State B) Method: EM (single particle) / Resolution: 3.1 Å EMDB-51053: Local B-factor-filtered map of an assembly intermediate of human mitochondrial ribosome small subunit (State A) Method: EM (single particle) / Resolution: 3.4 Å EMDB-51054: Translation-initiation state of human mitochondrial ribosome small subunit (State F) Method: EM (single particle) / Resolution: 3.2 Å EMDB-51055: Translation-initiation state of human mitochondrial ribosome small subunit (State F) Method: EM (single particle) / Resolution: 3.2 Å EMDB-51056: Assembly intermediate of human mitochondrial ribosome small subunit (State C) Method: EM (single particle) / Resolution: 3.1 Å EMDB-51057: Assembly intermediate of human mitochondrial ribosome small subunit (State C) Method: EM (single particle) / Resolution: 3.2 Å EMDB-51058: Assembly intermediate of human mitochondrial ribosome small subunit (State C) Method: EM (single particle) / Resolution: 3.1 Å EMDB-51059: Assembly intermediate of human mitochondrial ribosome small subunit (State C) Method: EM (single particle) / Resolution: 3.0 Å EMDB-51060: Assembly intermediate of human mitochondrial ribosome small subunit (State B) Method: EM (single particle) / Resolution: 3.4 Å EMDB-51061: Assembly intermediate of human mitochondrial ribosome small subunit (State B) Method: EM (single particle) / Resolution: 3.2 Å EMDB-51062: Assembly intermediate of human mitochondrial ribosome small subunit (State B) Method: EM (single particle) / Resolution: 3.1 Å EMDB-51063: Assembly intermediate of human mitochondrial ribosome small subunit (State B) Method: EM (single particle) / Resolution: 3.0 Å EMDB-51064: Head-focused refinement of an assembly intermediate of human mitochondrial ribosome small subunit (State A) Method: EM (single particle) / Resolution: 3.7 Å EMDB-51065: Body-focused refinement of an assembly intermediate of human mitochondrial ribosome small subunit (State A) Method: EM (single particle) / Resolution: 3.4 Å EMDB-51066: Consensus refinement of an assembly intermediate of human mitochondrial ribosome small subunit (State A) Method: EM (single particle) / Resolution: 3.2 Å 51083 9g5b EMDB-51083, PDB-9g5b: Assembly intermediate of human mitochondrial ribosome small subunit (State A) Method: EM (single particle) / Resolution: 3.2 Å 51084 9g5c EMDB-51084, PDB-9g5c: Assembly intermediate of human mitochondrial ribosome small subunit (State B) Method: EM (single particle) / Resolution: 3.0 Å 51085 9g5d EMDB-51085, PDB-9g5d: Assembly intermediate of human mitochondrial ribosome small subunit (State C) Method: EM (single particle) / Resolution: 3.0 Å 51086 9g5e EMDB-51086, PDB-9g5e: Translation-initiation state of human mitochondrial ribosome small subunit (State F) Method: EM (single particle) / Resolution: 3.2 Å EMDB-52062: Translation-initiation state of human mitochondrial ribosome small subunit (State D) Method: EM (single particle) / Resolution: 3.1 Å EMDB-52063: Translation-initiation state of human mitochondrial ribosome small subunit (State D) Method: EM (single particle) / Resolution: 3.3 Å EMDB-52064: Translation-initiation state of human mitochondrial ribosome small subunit (State E) Method: EM (single particle) / Resolution: 3.4 Å EMDB-52065: Translation-initiation state of human mitochondrial ribosome small subunit (State E) Method: EM (single particle) / Resolution: 3.7 Å EMDB-52066: Translation-initiation state of human mitochondrial ribosome small subunit (State G) Method: EM (single particle) / Resolution: 3.2 Å EMDB-52067: Translation-initiation state of human mitochondrial ribosome small subunit (State G) Method: EM (single particle) / Resolution: 3.2 Å EMDB-52068: Translation-initiation state of human mitochondrial ribosome small subunit (State G) Method: EM (single particle) / Resolution: 3.4 Å 52117 9hfm EMDB-52117, PDB-9hfm: Translation-initiation state of human mitochondrial ribosome small subunit (State D) Method: EM (single particle) / Resolution: 3.0 Å 52118 9hfn EMDB-52118, PDB-9hfn: Translation-initiation state of human mitochondrial ribosome small subunit (State E) Method: EM (single particle) / Resolution: 3.3 Å 52119 9hfo EMDB-52119, PDB-9hfo: Translation-initiation state of human mitochondrial ribosome small subunit (State G) Method: EM (single particle) / Resolution: 3.1 Å
Chemicals	ChemComp-MG: Unknown entry ChemComp-ZN: Unknown entry ChemComp-FES: FE2/S2 (INORGANIC) CLUSTER ChemComp-ATP: ADENOSINE-5'-TRIPHOSPHATE / ATP, energy-carrying moleculeYM ChemComp-GDP: GUANOSINE-5'-DIPHOSPHATE / GDP, energy-carrying moleculeYM ChemComp-K: Unknown entry ChemComp-SAH: S-ADENOSYL-L-HOMOCYSTEINE ChemComp-NAD: NICOTINAMIDE-ADENINE-DINUCLEOTIDE / NADYM ChemComp-GTP: GUANOSINE-5'-TRIPHOSPHATE / GTP, energy-carrying moleculeYM
Source	homo sapiens (human)
Keywords	RIBOSOME / mitochondrial ribosomal small subunit / assembly intermediate / MTG3 / single-particle cryo-EM / immature h44 / translation-initiation intermediate