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- EMDB-51085: Assembly intermediate of human mitochondrial ribosome small subun... -

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Basic information

Entry
Database: EMDB / ID: EMD-51085
TitleAssembly intermediate of human mitochondrial ribosome small subunit (State C)
Map dataComposite map of state C
Sample
  • Complex: Assembly intermediate of human mitochondrial ribosome small subunit (State C)
    • Protein or peptide: x 31 types
  • RNA: x 1 types
  • Ligand: x 8 types
Keywordsmitochondrial ribosomal small subunit / assembly intermediate / immature h44 / single-particle cryo-EM / RIBOSOME
Function / homology
Function and homology information


rRNA (cytosine-N4-)-methyltransferase activity / mitochondrial ribosome assembly / Mitochondrial translation elongation / Mitochondrial translation termination / Mitochondrial translation initiation / negative regulation of mitotic nuclear division / rRNA base methylation / mitochondrial ribosome / mitochondrial small ribosomal subunit / mitochondrial translation ...rRNA (cytosine-N4-)-methyltransferase activity / mitochondrial ribosome assembly / Mitochondrial translation elongation / Mitochondrial translation termination / Mitochondrial translation initiation / negative regulation of mitotic nuclear division / rRNA base methylation / mitochondrial ribosome / mitochondrial small ribosomal subunit / mitochondrial translation / apoptotic mitochondrial changes / positive regulation of proteolysis / ribosomal small subunit binding / Mitochondrial protein degradation / Transferases; Transferring one-carbon groups; Methyltransferases / apoptotic signaling pathway / rRNA processing / cell junction / regulation of translation / ribosomal small subunit assembly / small ribosomal subunit / small ribosomal subunit rRNA binding / nuclear membrane / Hydrolases; Acting on acid anhydrides; Acting on GTP to facilitate cellular and subcellular movement / tRNA binding / cell population proliferation / mitochondrial inner membrane / rRNA binding / structural constituent of ribosome / ribosome / translation / mitochondrial matrix / protein domain specific binding / intracellular membrane-bounded organelle / mRNA binding / GTP binding / nucleolus / mitochondrion / RNA binding / nucleoplasm / nucleus / plasma membrane / cytoplasm / cytosol
Similarity search - Function
Ribosomal RNA small subunit methyltransferase H / S-adenosyl-L-methionine-dependent methyltransferase, MraW, recognition domain superfamily / MraW methylase family / Putative ribosome-binding factor A, mitochondrial / Ribosome-binding factor A, conserved site / Ribosome-binding factor A signature. / Ribosome-binding factor A / Ribosome-binding factor A domain superfamily / Ribosome-binding factor A / 28S ribosomal protein S26 ...Ribosomal RNA small subunit methyltransferase H / S-adenosyl-L-methionine-dependent methyltransferase, MraW, recognition domain superfamily / MraW methylase family / Putative ribosome-binding factor A, mitochondrial / Ribosome-binding factor A, conserved site / Ribosome-binding factor A signature. / Ribosome-binding factor A / Ribosome-binding factor A domain superfamily / Ribosome-binding factor A / 28S ribosomal protein S26 / Mitochondrial ribosome subunit S26 / 28S ribosomal protein S27, mitochondrial / MRPS27/PTCD2 / Mitochondrial 28S ribosomal protein S27 / Ribosomal protein S22, mitochondrial / Ribosomal protein S23, mitochondrial / Ribosomal protein S23/S25, mitochondrial / Mitochondrial 28S ribosomal protein S31 / : / Mitochondrial 28S ribosomal protein S22 / Mitochondrial ribosomal protein S23 / Mitochondrial 28S ribosomal protein S31 / Ribosomal protein S29, mitochondrial / Ribosomal protein S28, mitochondrial / Mitochondrial 28S ribosomal protein S34 / 28S ribosomal protein S10, mitochondrial / Mitochondrial ribosomal protein MRP-S35 / Mitochondrial 28S ribosomal protein S34 / 28S ribosomal protein S24, mitochondrial / Pentatricopeptide repeat domain-containing protein 3 / 28S ribosomal protein S17, mitochondrial / 28S ribosomal protein S18b, mitochondrial / : / : / Mitochondrial ribosome subunit S24 / Small ribosomal subunit protein uS5m, N-terminal / Small ribosomal subunit protein mS39, PPR / 28S ribosomal protein S25, mitochondrial / Pentatricopeptide repeat domain / Ribosomal protein S27/S33, mitochondrial / Ribosomal protein S24/S35, mitochondrial / Mitochondrial ribosomal subunit S27 / Ribosomal protein S24/S35, mitochondrial, conserved domain / Mitochondrial ribosomal subunit protein / Pentatricopeptide (PPR) repeat profile. / Ribosomal protein S23/S29, mitochondrial / Mitochondrial ribosomal death-associated protein 3 / Pentatricopeptide repeat / Mitochondrial mRNA-processing protein COX24, C-terminal / Mitochondrial mRNA-processing protein COX24, C-terminal / Mitochondrial domain of unknown function (DUF1713) / Mitochondrial ribosomal protein L51 / S25 / CI-B8 domain / Ribosomal protein/NADH dehydrogenase domain / Mitochondrial ribosomal protein L51 / S25 / CI-B8 domain / Ribosomal protein S21 superfamily / Ribosomal protein S21 / Ribosomal protein S21 / Ribosomal protein S2, bacteria/mitochondria/plastid / Ribosomal protein S9, bacterial/plastid / Ribosomal protein S18, conserved site / Ribosomal protein S18 signature. / Ribosomal protein S16 / Ribosomal protein S16 domain superfamily / Ribosomal protein S16 / Ribosomal protein S15, bacterial-type / Ribosomal protein S6 / Ribosomal protein S6 / Ribosomal protein S6 superfamily / Ribosomal protein S12, bacterial-type / Translation elongation factor EF1B/ribosomal protein S6 / Ribosomal protein S18 / Ribosomal protein S18 / Ribosomal protein S18 superfamily / Ribosomal protein S2 signature 1. / Ribosomal protein S5, N-terminal, conserved site / Ribosomal protein S5 signature. / Ribosomal protein S2, conserved site / Ribosomal protein S2 / Ribosomal protein S2, flavodoxin-like domain superfamily / Ribosomal protein S2 / Ribosomal protein S5 / S5 double stranded RNA-binding domain profile. / Ribosomal protein S5, N-terminal / Ribosomal protein S5, C-terminal / Ribosomal protein S5, N-terminal domain / Ribosomal protein S5, C-terminal domain / Ribosomal protein S14 / Ribosomal protein S14p/S29e / K homology domain-like, alpha/beta / Tetratricopeptide-like helical domain superfamily / Ribosomal S11, conserved site / Ribosomal protein S11 signature. / Ribosomal protein S10p/S20e / Ribosomal protein S9, conserved site / Ribosomal protein S9 signature. / Ribosomal protein S11 / Ribosomal protein S10 domain / Ribosomal protein S10 domain superfamily / Ribosomal protein S10p/S20e / Ribosomal protein S12 signature.
Similarity search - Domain/homology
12S rRNA N(4)-cytidine methyltransferase METTL15 / Small ribosomal subunit protein uS12m / Small ribosomal subunit protein uS14m / Small ribosomal subunit protein mS29 / Small ribosomal subunit protein mS22 / Small ribosomal subunit protein mS25 / Small ribosomal subunit protein uS10m / Small ribosomal subunit protein mS35 / Small ribosomal subunit protein uS5m / Small ribosomal subunit protein uS11m ...12S rRNA N(4)-cytidine methyltransferase METTL15 / Small ribosomal subunit protein uS12m / Small ribosomal subunit protein uS14m / Small ribosomal subunit protein mS29 / Small ribosomal subunit protein mS22 / Small ribosomal subunit protein mS25 / Small ribosomal subunit protein uS10m / Small ribosomal subunit protein mS35 / Small ribosomal subunit protein uS5m / Small ribosomal subunit protein uS11m / Small ribosomal subunit protein uS15m / Small ribosomal subunit protein bS21m / Small ribosomal subunit protein mS34 / Small ribosomal subunit protein bS6m / Small ribosomal subunit protein uS9m / Putative ribosome-binding factor A, mitochondrial / Small ribosomal subunit protein mS27 / Small ribosomal subunit protein mS31 / Small ribosomal subunit protein uS3m / Small ribosomal subunit protein mS39 / Small ribosomal subunit protein mS26 / Small ribosomal subunit protein mS38 / Small ribosomal subunit protein mS33 / Small ribosomal subunit protein bS1m / Small ribosomal subunit protein uS17m / Small ribosomal subunit protein uS7m / Small ribosomal subunit protein uS2m / Small ribosomal subunit protein bS16m / Small ribosomal subunit protein bS18m / Small ribosomal subunit protein mS23 / Small ribosomal subunit protein mS40
Similarity search - Component
Biological speciesHomo sapiens (human)
Methodsingle particle reconstruction / cryo EM / Resolution: 3.0 Å
AuthorsFinke AF / Heinrichs M / Aibara S / Richter-Dennerlein R / Hillen HS
Funding support Germany, European Union, 6 items
OrganizationGrant numberCountry
German Research Foundation (DFG)EXC 2067/1- 390729940 Germany
German Research Foundation (DFG)RI 2715/1- 1 Germany
German Research Foundation (DFG)FOR2848 Germany
German Research Foundation (DFG)SFB1190 Germany
German Research Foundation (DFG)SFB1565 Germany
European Research Council (ERC)101116869European Union
CitationJournal: Nat Commun / Year: 2025
Title: Coupling of ribosome biogenesis and translation initiation in human mitochondria.
Authors: Marleen Heinrichs / Anna Franziska Finke / Shintaro Aibara / Angelique Krempler / Angela Boshnakovska / Peter Rehling / Hauke S Hillen / Ricarda Richter-Dennerlein /
Abstract: Biogenesis of mitoribosomes requires dedicated chaperones, RNA-modifying enzymes, and GTPases, and defects in mitoribosome assembly lead to severe mitochondriopathies in humans. Here, we characterize ...Biogenesis of mitoribosomes requires dedicated chaperones, RNA-modifying enzymes, and GTPases, and defects in mitoribosome assembly lead to severe mitochondriopathies in humans. Here, we characterize late-step assembly states of the small mitoribosomal subunit (mtSSU) by combining genetic perturbation and mutagenesis analysis with biochemical and structural approaches. Isolation of native mtSSU biogenesis intermediates via a FLAG-tagged variant of the GTPase MTG3 reveals three distinct assembly states, which show how factors cooperate to mature the 12S rRNA. In addition, we observe four distinct primed initiation mtSSU states with an incompletely matured rRNA, suggesting that biogenesis and translation initiation are not mutually exclusive processes but can occur simultaneously. Together, these results provide insights into mtSSU biogenesis and suggest a functional coupling between ribosome biogenesis and translation initiation in human mitochondria.
History
DepositionJul 16, 2024-
Header (metadata) releaseApr 23, 2025-
Map releaseApr 23, 2025-
UpdateApr 30, 2025-
Current statusApr 30, 2025Processing site: PDBe / Status: Released

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Structure visualization

Supplemental images

emd_51085.png

Downloads & links

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Map

FileDownload / File: emd_51085.map.gz / Format: CCP4 / Size: 325 MB / Type: IMAGE STORED AS FLOATING POINT NUMBER (4 BYTES)
AnnotationComposite map of state C
Projections & slices

Image control

Size
Brightness
Contrast
Others
AxesZ (Sec.)Y (Row.)X (Col.)
1.05 Å/pix.
x 440 pix.
= 462. Å		1.05 Å/pix.
x 440 pix.
= 462. Å		1.05 Å/pix.
x 440 pix.
= 462. Å

Surface

Projections

Slices (1/3)

Slices (1/2)

Slices (2/3)

Images are generated by Spider.

Voxel sizeX=Y=Z: 1.05 Å
Density

Histogram

Histogram (log scale)
Contour LevelBy AUTHOR: 0.39
Minimum - Maximum-0.1963171 - 2.1126633
Average (Standard dev.)0.015008831 (±0.053760428)
SymmetrySpace group: 1
Details

EMDB XML:

Map geometry
Axis orderXYZ
Origin000
Dimensions440440440
Spacing440440440
CellA=B=C: 461.99997 Å
α=β=γ: 90.0 °

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Supplemental data

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Sample components

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Entire : Assembly intermediate of human mitochondrial ribosome small subun...

EntireName: Assembly intermediate of human mitochondrial ribosome small subunit (State C)
Components
  • Complex: Assembly intermediate of human mitochondrial ribosome small subunit (State C)
    • Protein or peptide: 28S ribosomal protein S34, mitochondrial
    • Protein or peptide: 28S ribosomal protein S35, mitochondrial
    • Protein or peptide: Aurora kinase A-interacting protein
    • Protein or peptide: Pentatricopeptide repeat domain-containing protein 3, mitochondrial
    • Protein or peptide: 28S ribosomal protein S2, mitochondrial
    • Protein or peptide: 28S ribosomal protein S24, mitochondrial
    • Protein or peptide: 28S ribosomal protein S5, mitochondrial
    • Protein or peptide: 28S ribosomal protein S6, mitochondrial
    • Protein or peptide: 28S ribosomal protein S7, mitochondrial
    • Protein or peptide: 28S ribosomal protein S9, mitochondrial
    • Protein or peptide: 28S ribosomal protein S10, mitochondrial
    • Protein or peptide: 28S ribosomal protein S11, mitochondrial
    • Protein or peptide: 28S ribosomal protein S12, mitochondrial
    • Protein or peptide: 28S ribosomal protein S14, mitochondrial
    • Protein or peptide: 28S ribosomal protein S15, mitochondrial
    • Protein or peptide: 28S ribosomal protein S16, mitochondrial
    • Protein or peptide: 28S ribosomal protein S17, mitochondrial
    • Protein or peptide: 28S ribosomal protein S18b, mitochondrial
    • Protein or peptide: 28S ribosomal protein S18c, mitochondrial
    • Protein or peptide: 28S ribosomal protein S21, mitochondrial
    • Protein or peptide: 28S ribosomal protein S22, mitochondrial
    • Protein or peptide: 28S ribosomal protein S23, mitochondrial
    • Protein or peptide: 28S ribosomal protein S25, mitochondrial
    • Protein or peptide: 28S ribosomal protein S26, mitochondrial
    • Protein or peptide: 28S ribosomal protein S27, mitochondrial
    • Protein or peptide: 28S ribosomal protein S28, mitochondrial
    • Protein or peptide: 28S ribosomal protein S29, mitochondrial
    • Protein or peptide: 28S ribosomal protein S31, mitochondrial
    • Protein or peptide: 28S ribosomal protein S33, mitochondrial
    • Protein or peptide: Putative ribosome-binding factor A, mitochondrial
    • Protein or peptide: 12S rRNA N4-methylcytidine (m4C) methyltransferase
  • RNA: 12S mitochondrial rRNA
  • Ligand: MAGNESIUM ION
  • Ligand: POTASSIUM ION
  • Ligand: NICOTINAMIDE-ADENINE-DINUCLEOTIDE
  • Ligand: ZINC ION
  • Ligand: FE2/S2 (INORGANIC) CLUSTER
  • Ligand: GUANOSINE-5'-DIPHOSPHATE
  • Ligand: ADENOSINE-5'-TRIPHOSPHATE
  • Ligand: S-ADENOSYL-L-HOMOCYSTEINE

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Supramolecule #1: Assembly intermediate of human mitochondrial ribosome small subun...

SupramoleculeName: Assembly intermediate of human mitochondrial ribosome small subunit (State C)
type: complex / ID: 1 / Parent: 0 / Macromolecule list: #1-#4, #6-#32
Details: Early assembly mtSSU intermediate in complex with METTL15, and mtRBFA(out), and missing mS37, with immature h44 and partially h24.
Source (natural)Organism: Homo sapiens (human) / Strain: HEK293T
Molecular weightTheoretical: 990 KDa

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Macromolecule #1: 28S ribosomal protein S34, mitochondrial

MacromoleculeName: 28S ribosomal protein S34, mitochondrial / type: protein_or_peptide / ID: 1 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 25.695549 KDa
SequenceString: MARKKVRPRL IAELARRVRA LREQLNRPRD SQLYAVDYET LTRPFSGRRL PVRAWADVRR ESRLLQLLGR LPLFGLGRLV TRKSWLWQH DEPCYWRLTR VRPDYTAQNL DHGKAWGILT FKGKTESEAR EIEHVMYHDW RLVPKHEEEA FTAFTPAPED S LASVPYPP ...String:
MARKKVRPRL IAELARRVRA LREQLNRPRD SQLYAVDYET LTRPFSGRRL PVRAWADVRR ESRLLQLLGR LPLFGLGRLV TRKSWLWQH DEPCYWRLTR VRPDYTAQNL DHGKAWGILT FKGKTESEAR EIEHVMYHDW RLVPKHEEEA FTAFTPAPED S LASVPYPP LLRAMIIAER QKNGDTSTEE PMLNVQRIRM EPWDYPAKQE DKGRAKGTPV

UniProtKB: Small ribosomal subunit protein mS34

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Macromolecule #2: 28S ribosomal protein S35, mitochondrial

MacromoleculeName: 28S ribosomal protein S35, mitochondrial / type: protein_or_peptide / ID: 2 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 36.898328 KDa
SequenceString: MAAAALPAWL SLQSRARTLR AFSTAVYSAT PVPTPSLPER TPGNERPPRR KALPPRTEKM AVDQDWPSVY PVAAPFKPSA VPLPVRMGY PVKKGVPMAK EGNLELLKIP NFLHLTPVAI KKHCEALKDF CTEWPAALDS DEKCEKHFPI EIDSTDYVSS G PSVRNPRA ...String:
MAAAALPAWL SLQSRARTLR AFSTAVYSAT PVPTPSLPER TPGNERPPRR KALPPRTEKM AVDQDWPSVY PVAAPFKPSA VPLPVRMGY PVKKGVPMAK EGNLELLKIP NFLHLTPVAI KKHCEALKDF CTEWPAALDS DEKCEKHFPI EIDSTDYVSS G PSVRNPRA RVVVLRVKLS SLNLDDHAKK KLIKLVGERY CKTTDVLTIK TDRCPLRRQN YDYAVYLLTV LYHESWNTEE WE KSKTEAD MEEYIWENSS SERNILETLL QMKAAEKNME INKEELLGTK EIEEYKKSVV SLKNEEENEN SISQYKESVK RLL NVT

UniProtKB: Small ribosomal subunit protein mS35

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Macromolecule #3: Aurora kinase A-interacting protein

MacromoleculeName: Aurora kinase A-interacting protein / type: protein_or_peptide / ID: 3 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 22.395326 KDa
SequenceString: MLLGRLTSQL LRAVPWAGGR PPWPVSGVLG SRVCGPLYST SPAGPGRAAS LPRKGAQLEL EEMLVPRKMS VSPLESWLTA RCFLPRLDT GTAGTVAPPQ SYQCPPSQIG EGAEQGDEGV ADAPQIQCKN VLKIRRRKMN HHKYRKLVKK TRFLRRKVQE G RLRRKQIK ...String:
MLLGRLTSQL LRAVPWAGGR PPWPVSGVLG SRVCGPLYST SPAGPGRAAS LPRKGAQLEL EEMLVPRKMS VSPLESWLTA RCFLPRLDT GTAGTVAPPQ SYQCPPSQIG EGAEQGDEGV ADAPQIQCKN VLKIRRRKMN HHKYRKLVKK TRFLRRKVQE G RLRRKQIK FEKDLRRIWL KAGLKEAPEG WQTPKIYLRG K

UniProtKB: Small ribosomal subunit protein mS38

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Macromolecule #4: Pentatricopeptide repeat domain-containing protein 3, mitochondrial

MacromoleculeName: Pentatricopeptide repeat domain-containing protein 3, mitochondrial
type: protein_or_peptide / ID: 4 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 78.648547 KDa
SequenceString: MAVVSAVRWL GLRSRLGQPL TGRRAGLCEQ ARSCRFYSGS ATLSKVEGTD VTGIEEVVIP KKKTWDKVAV LQALASTVNR DTTAVPYVF QDDPYLMPAS SLESRSFLLA KKSGENVAKF IINSYPKYFQ KDIAEPHIPC LMPEYFEPQI KDISEAALKE R IELRKVKA ...String:
MAVVSAVRWL GLRSRLGQPL TGRRAGLCEQ ARSCRFYSGS ATLSKVEGTD VTGIEEVVIP KKKTWDKVAV LQALASTVNR DTTAVPYVF QDDPYLMPAS SLESRSFLLA KKSGENVAKF IINSYPKYFQ KDIAEPHIPC LMPEYFEPQI KDISEAALKE R IELRKVKA SVDMFDQLLQ AGTTVSLETT NSLLDLLCYY GDQEPSTDYH FQQTGQSEAL EEENDETSRR KAGHQFGVTW RA KNNAERI FSLMPEKNEH SYCTMIRGMV KHRAYEQALN LYTELLNNRL HADVYTFNAL IEATVCAINE KFEEKWSKIL ELL RHMVAQ KVKPNLQTFN TILKCLRRFH VFARSPALQV LREMKAIGIE PSLATYHHII RLFDQPGDPL KRSSFIIYDI MNEL MGKRF SPKDPDDDKF FQSAMSICSS LRDLELAYQV HGLLKTGDNW KFIGPDQHRN FYYSKFFDLI CLMEQIDVTL KWYED LIPS AYFPHSQTMI HLLQALDVAN RLEVIPKIWK DSKEYGHTFR SDLREEILML MARDKHPPEL QVAFADCAAD IKSAYE SQP IRQTAQDWPA TSLNCIAILF LRAGRTQEAW KMLGLFRKHN KIPRSELLNE LMDSAKVSNS PSQAIEVVEL ASAFSLP IC EGLTQRVMSD FAINQEQKEA LSNLTALTSD SDTDSSSDSD SDTSEGK

UniProtKB: Small ribosomal subunit protein mS39

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Macromolecule #6: 28S ribosomal protein S2, mitochondrial

MacromoleculeName: 28S ribosomal protein S2, mitochondrial / type: protein_or_peptide / ID: 6 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 33.298238 KDa
SequenceString: MATSSAALPR ILGAGARAPS RWLGFLGKAT PRPARPSRRT LGSATALMIR ESEDSTDFND KILNEPLKHS DFFNVKELFS VRSLFDARV HLGHKAGCRH RFMEPYIFGS RLDHDIIDLE QTATHLQLAL NFTAHMAYRK GIILFISRNR QFSYLIENMA R DCGEYAHT ...String:
MATSSAALPR ILGAGARAPS RWLGFLGKAT PRPARPSRRT LGSATALMIR ESEDSTDFND KILNEPLKHS DFFNVKELFS VRSLFDARV HLGHKAGCRH RFMEPYIFGS RLDHDIIDLE QTATHLQLAL NFTAHMAYRK GIILFISRNR QFSYLIENMA R DCGEYAHT RYFRGGMLTN ARLLFGPTVR LPDLIIFLHT LNNIFEPHVA VRDAAKMNIP TVGIVDTNCN PCLITYPVPG ND DSPLAVH LYCRLFQTAI TRAKEKRQQV EALYRLQGQK EPGDQGPAHP PGADMSHSL

UniProtKB: Small ribosomal subunit protein uS2m

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Macromolecule #7: 28S ribosomal protein S24, mitochondrial

MacromoleculeName: 28S ribosomal protein S24, mitochondrial / type: protein_or_peptide / ID: 7 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 19.046164 KDa
SequenceString:
MAASVCSGLL GPRVLSWSRE LPCAWRALHT SPVCAKNRAA RVRVSKGDKP VTYEEAHAPH YIAHRKGWLS LHTGNLDGED HAAERTVED VFLRKFMWGT FPGCLADQLV LKRRGNQLEI CAVVLRQLSP HKYYFLVGYS ETLLSYFYKC PVRLHLQTVP S KVVYKYL

UniProtKB: Small ribosomal subunit protein uS3m

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Macromolecule #8: 28S ribosomal protein S5, mitochondrial

MacromoleculeName: 28S ribosomal protein S5, mitochondrial / type: protein_or_peptide / ID: 8 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 48.094594 KDa
SequenceString: MATAVRAVGC LPVLCSGTAG HLLGRQCSLN TLPAASILAW KSVLGNGHLS SLGTRDTHPY ASLSRALQTQ CCISSPSHLM SQQYRPYSF FTKLTADELW KGALAETGAG AKKGRGKRTK KKKRKDLNRG QIIGEGRYGF LWPGLNVPLM KNGAVQTIAQ R SKEEQEKV ...String:
MATAVRAVGC LPVLCSGTAG HLLGRQCSLN TLPAASILAW KSVLGNGHLS SLGTRDTHPY ASLSRALQTQ CCISSPSHLM SQQYRPYSF FTKLTADELW KGALAETGAG AKKGRGKRTK KKKRKDLNRG QIIGEGRYGF LWPGLNVPLM KNGAVQTIAQ R SKEEQEKV EADMIQQREE WDRKKKMKVK RERGWSGNSW GGISLGPPDP GPCGETYEDF DTRILEVRNV FTMTAKEGRK KS IRVLVAV GNGKGAAGFS IGKATDRMDA FRKAKNRAVH HLHYIERYED HTIFHDISLR FKRTHIKMKK QPKGYGLRCH RAI ITICRL IGIKDMYAKV SGSINMLSLT QGLFRGLSRQ ETHQQLADKK GLHVVEIREE CGPLPIVVAS PRGPLRKDPE PEDE VPDVK LDWEDVKTAQ GMKRSVWSNL KRAAT

UniProtKB: Small ribosomal subunit protein uS5m

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Macromolecule #9: 28S ribosomal protein S6, mitochondrial

MacromoleculeName: 28S ribosomal protein S6, mitochondrial / type: protein_or_peptide / ID: 9 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 14.250588 KDa
SequenceString:
MPRYELALIL KAMQRPETAA TLKRTIEALM DRGAIVRDLE NLGERALPYR ISAHSQQHNR GGYFLVDFYA PTAAVESMVE HLSRDIDVI RGNIVKHPLT QELKECEGIV PVPLAEKLYS TKKRKK

UniProtKB: Small ribosomal subunit protein bS6m

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Macromolecule #10: 28S ribosomal protein S7, mitochondrial

MacromoleculeName: 28S ribosomal protein S7, mitochondrial / type: protein_or_peptide / ID: 10 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 28.186154 KDa
SequenceString: MAAPAVKVAR GWSGLALGVR RAVLQLPGLT QVRWSRYSPE FKDPLIDKEY YRKPVEELTE EEKYVRELKK TQLIKAAPAG KTSSVFEDP VISKFTNMMM IGGNKVLARS LMIQTLEAVK RKQFEKYHAA SAEEQATIER NPYTIFHQAL KNCEPMIGLV P ILKGGRFY ...String:
MAAPAVKVAR GWSGLALGVR RAVLQLPGLT QVRWSRYSPE FKDPLIDKEY YRKPVEELTE EEKYVRELKK TQLIKAAPAG KTSSVFEDP VISKFTNMMM IGGNKVLARS LMIQTLEAVK RKQFEKYHAA SAEEQATIER NPYTIFHQAL KNCEPMIGLV P ILKGGRFY QVPVPLPDRR RRFLAMKWMI TECRDKKHQR TLMPEKLSHK LLEAFHNQGP VIKRKHDLHK MAEANRALAH YR WW

UniProtKB: Small ribosomal subunit protein uS7m

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Macromolecule #11: 28S ribosomal protein S9, mitochondrial

MacromoleculeName: 28S ribosomal protein S9, mitochondrial / type: protein_or_peptide / ID: 11 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 45.909859 KDa
SequenceString: MAAPCVSYGG AVSYRLLLWG RGSLARKQGL WKTAAPELQT NVRSQILRLR HTAFVIPKKN VPTSKRETYT EDFIKKQIEE FNIGKRHLA NMMGEDPETF TQEDIDRAIA YLFPSGLFEK RARPVMKHPE QIFPRQRAIQ WGEDGRPFHY LFYTGKQSYY S LMHDVYGM ...String:
MAAPCVSYGG AVSYRLLLWG RGSLARKQGL WKTAAPELQT NVRSQILRLR HTAFVIPKKN VPTSKRETYT EDFIKKQIEE FNIGKRHLA NMMGEDPETF TQEDIDRAIA YLFPSGLFEK RARPVMKHPE QIFPRQRAIQ WGEDGRPFHY LFYTGKQSYY S LMHDVYGM LLNLEKHQSH LQAKSLLPEK TVTRDVIGSR WLIKEELEEM LVEKLSDLDY MQFIRLLEKL LTSQCGAAEE EF VQRFRRS VTLESKKQLI EPVQYDEQGM AFSKSEGKRK TAKAEAIVYK HGSGRIKVNG IDYQLYFPIT QDREQLMFPF HFV DRLGKH DVTCTVSGGG RSAQAGAIRL AMAKALCSFV TEDEVEWMRQ AGLLTTDPRV RERKKPGQEG ARRKFTWKKR

UniProtKB: Small ribosomal subunit protein uS9m

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Macromolecule #12: 28S ribosomal protein S10, mitochondrial

MacromoleculeName: 28S ribosomal protein S10, mitochondrial / type: protein_or_peptide / ID: 12 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 23.0335 KDa
SequenceString: MAARTAFGAV CRRLWQGLGN FSVNTSKGNT AKNGGLLLST NMKWVQFSNL HVDVPKDLTK PVVTISDEPD ILYKRLSVLV KGHDKAVLD SYEYFAVLAA KELGISIKVH EPPRKIERFT LLQSVHIYKK HRVQYEMRTL YRCLELEHLT GSTADVYLEY I QRNLPEGV ...String:
MAARTAFGAV CRRLWQGLGN FSVNTSKGNT AKNGGLLLST NMKWVQFSNL HVDVPKDLTK PVVTISDEPD ILYKRLSVLV KGHDKAVLD SYEYFAVLAA KELGISIKVH EPPRKIERFT LLQSVHIYKK HRVQYEMRTL YRCLELEHLT GSTADVYLEY I QRNLPEGV AMEVTKTQLE QLPEHIKEPI WETLSEEKEE SKS

UniProtKB: Small ribosomal subunit protein uS10m

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Macromolecule #13: 28S ribosomal protein S11, mitochondrial

MacromoleculeName: 28S ribosomal protein S11, mitochondrial / type: protein_or_peptide / ID: 13 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 20.648793 KDa
SequenceString: MQAVRNAGSR FLRSWTWPQT AGRVVARTPA GTICTGARQL QDAAAKQKVE QNAAPSHTKF SIYPPIPGEE SSLRWAGKKF EEIPIAHIK ASHNNTQIQV VSASNEPLAF ASCGTEGFRN AKKGTGIAAQ TAGIAAAARA KQKGVIHIRV VVKGLGPGRL S AMHGLIMG ...String:
MQAVRNAGSR FLRSWTWPQT AGRVVARTPA GTICTGARQL QDAAAKQKVE QNAAPSHTKF SIYPPIPGEE SSLRWAGKKF EEIPIAHIK ASHNNTQIQV VSASNEPLAF ASCGTEGFRN AKKGTGIAAQ TAGIAAAARA KQKGVIHIRV VVKGLGPGRL S AMHGLIMG GLEVISITDN TPIPHNGCRP RKARKL

UniProtKB: Small ribosomal subunit protein uS11m

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Macromolecule #14: 28S ribosomal protein S12, mitochondrial

MacromoleculeName: 28S ribosomal protein S12, mitochondrial / type: protein_or_peptide / ID: 14 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 15.200947 KDa
SequenceString:
MSWSGLLHGL NTSLTCGPAL VPRLWATCSM ATLNQMHRLG PPKRPPRKLG PTEGRPQLKG VVLCTFTRKP KKPNSANRKC CRVRLSTGR EAVCFIPGEG HTLQEHQIVL VEGGRTQDLP GVKLTVVRGK YDCGHVQKK

UniProtKB: Small ribosomal subunit protein uS12m

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Macromolecule #15: 28S ribosomal protein S14, mitochondrial

MacromoleculeName: 28S ribosomal protein S14, mitochondrial / type: protein_or_peptide / ID: 15 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 15.168788 KDa
SequenceString:
MAAFMLGSLL RTFKQMVPSS ASGQVRSHYV DWRMWRDVKR RKMAYEYADE RLRINSLRKN TILPKILQDV ADEEIAALPR DSCPVRIRN RCVMTSRPRG VKRRWRLSRI VFRHLADHGQ LSGIQRATW

UniProtKB: Small ribosomal subunit protein uS14m

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Macromolecule #16: 28S ribosomal protein S15, mitochondrial

MacromoleculeName: 28S ribosomal protein S15, mitochondrial / type: protein_or_peptide / ID: 16 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 29.903293 KDa
SequenceString: MLRVAWRTLS LIRTRAVTQV LVPGLPGGGS AKFPFNQWGL QPRSLLLQAA RGYVVRKPAQ SRLDDDPPPS TLLKDYQNVP GIEKVDDVV KRLLSLEMAN KKEMLKIKQE QFMKKIVANP EDTRSLEARI IALSVKIRSY EEHLEKHRKD KAHKRYLLMS I DQRKKMLK ...String:
MLRVAWRTLS LIRTRAVTQV LVPGLPGGGS AKFPFNQWGL QPRSLLLQAA RGYVVRKPAQ SRLDDDPPPS TLLKDYQNVP GIEKVDDVV KRLLSLEMAN KKEMLKIKQE QFMKKIVANP EDTRSLEARI IALSVKIRSY EEHLEKHRKD KAHKRYLLMS I DQRKKMLK NLRNTNYDVF EKICWGLGIE YTFPPLYYRR AHRRFVTKKA LCIRVFQETQ KLKKRRRALK AAAAAQKQAK RR NPDSPAK AIPKTLKDSQ

UniProtKB: Small ribosomal subunit protein uS15m

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Macromolecule #17: 28S ribosomal protein S16, mitochondrial

MacromoleculeName: 28S ribosomal protein S16, mitochondrial / type: protein_or_peptide / ID: 17 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 15.371899 KDa
SequenceString:
MVHLTTLLCK AYRGGHLTIR LALGGCTNRP FYRIVAAHNK CPRDGRFVEQ LGSYDPLPNS HGEKLVALNL DRIRHWIGCG AHLSKPMEK LLGLAGFFPL HPMMITNAER LRRKRAREVL LASQKTDAEA TDTEATET

UniProtKB: Small ribosomal subunit protein bS16m

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Macromolecule #18: 28S ribosomal protein S17, mitochondrial

MacromoleculeName: 28S ribosomal protein S17, mitochondrial / type: protein_or_peptide / ID: 18 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 14.526078 KDa
SequenceString:
MSVVRSSVHA RWIVGKVIGT KMQKTAKVRV TRLVLDPYLL KYFNKRKTYF AHDALQQCTV GDIVLLRALP VPRAKHVKHE LAEIVFKVG KVIDPVTGKP CAGTTYLESP LSSETTQLSK NLEELNISSA Q

UniProtKB: Small ribosomal subunit protein uS17m

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Macromolecule #19: 28S ribosomal protein S18b, mitochondrial

MacromoleculeName: 28S ribosomal protein S18b, mitochondrial / type: protein_or_peptide / ID: 19 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 29.440629 KDa
SequenceString: MAASVLNTVL RRLPMLSLFR GSHRVQVPLQ TLCTKAPSEE DSLSSVPISP YKDEPWKYLE SEEYQERYGS RPVWADYRRN HKGGVPPQR TRKTCIRRNK VVGNPCPICR DHKLHVDFRN VKLLEQFVCA HTGIIFYAPY TGVCVKQHKR LTQAIQKARD H GLLIYHIP ...String:
MAASVLNTVL RRLPMLSLFR GSHRVQVPLQ TLCTKAPSEE DSLSSVPISP YKDEPWKYLE SEEYQERYGS RPVWADYRRN HKGGVPPQR TRKTCIRRNK VVGNPCPICR DHKLHVDFRN VKLLEQFVCA HTGIIFYAPY TGVCVKQHKR LTQAIQKARD H GLLIYHIP QVEPRDLDFS TSHGAVSATP PAPTLVSGDP WYPWYNWKQP PERELSRLRR LYQGHLQEES GPPPESMPKM PP RTPAEAS STGQTGPQSA L

UniProtKB: Small ribosomal subunit protein mS40

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Macromolecule #20: 28S ribosomal protein S18c, mitochondrial

MacromoleculeName: 28S ribosomal protein S18c, mitochondrial / type: protein_or_peptide / ID: 20 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 15.876786 KDa
SequenceString:
MAAVVAVCGG LGRKKLTHLV TAAVSLTHPG THTVLWRRGC SQQVSSNEDL PISMENPYKE PLKKCILCGK HVDYKNVQLL SQFVSPFTG CIYGRHITGL CGKKQKEITK AIKRAQIMGF MPVTYKDPAY LKDPKVCNIR YRE

UniProtKB: Small ribosomal subunit protein bS18m

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Macromolecule #21: 28S ribosomal protein S21, mitochondrial

MacromoleculeName: 28S ribosomal protein S21, mitochondrial / type: protein_or_peptide / ID: 21 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 10.764652 KDa
SequenceString:
MAKHLKFIAR TVMVQEGNVE SAYRTLNRIL TMDGLIEDIK HRRYYEKPCR RRQRESYERC RRIYNMEMAR KINFLMRKNR ADPWQGC

UniProtKB: Small ribosomal subunit protein bS21m

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Macromolecule #22: 28S ribosomal protein S22, mitochondrial

MacromoleculeName: 28S ribosomal protein S22, mitochondrial / type: protein_or_peptide / ID: 22 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 41.337316 KDa
SequenceString: MAPLGTTVLL WSLLRSSPGV ERVCFRARIQ PWHGGLLQPL PCSFEMGLPR RRFSSEAAES GSPETKKPTF MDEEVQSILT KMTGLNLQK TFKPAIQELK PPTYKLMTQA QLEEATRQAV EAAKVRLKMP PVLEERVPIN DVLAEDKILE GTETTKYVFT D ISYSIPHR ...String:
MAPLGTTVLL WSLLRSSPGV ERVCFRARIQ PWHGGLLQPL PCSFEMGLPR RRFSSEAAES GSPETKKPTF MDEEVQSILT KMTGLNLQK TFKPAIQELK PPTYKLMTQA QLEEATRQAV EAAKVRLKMP PVLEERVPIN DVLAEDKILE GTETTKYVFT D ISYSIPHR ERFIVVREPS GTLRKASWEE RDRMIQVYFP KEGRKILTPI IFKEENLRTM YSQDRHVDVL NLCFAQFEPD ST EYIKVHH KTYEDIDKRG KYDLLRSTRY FGGMVWYFVN NKKIDGLLID QIQRDLIDDA TNLVQLYHVL HPDGQSAQGA KDQ AAEGIN LIKVFAKTEA QKGAYIELTL QTYQEALSRH SAAS

UniProtKB: Small ribosomal subunit protein mS22

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Macromolecule #23: 28S ribosomal protein S23, mitochondrial

MacromoleculeName: 28S ribosomal protein S23, mitochondrial / type: protein_or_peptide / ID: 23 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 21.805664 KDa
SequenceString:
MAGSRLETVG SIFSRTRDLV RAGVLKEKPL WFDVYDAFPP LREPVFQRPR VRYGKAKAPI QDIWYHEDRI RAKFYSVYGS GQRAFDLFN PNFKSTCQRF VEKYTELQKL GETDEEKLFV ETGKALLAEG VILRRVGEAR TQHGGSHVSR KSEHLSVRPQ T ALEENETQ KEVPQDQHLE APADQSKGLL PP

UniProtKB: Small ribosomal subunit protein mS23

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Macromolecule #24: 28S ribosomal protein S25, mitochondrial

MacromoleculeName: 28S ribosomal protein S25, mitochondrial / type: protein_or_peptide / ID: 24 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 20.146428 KDa
SequenceString:
MPMKGRFPIR RTLQYLSQGN VVFKDSVKVM TVNYNTHGEL GEGARKFVFF NIPQIQYKNP WVQIMMFKNM TPSPFLRFYL DSGEQVLVD VETKSNKEIM EHIRKILGKN EETLREEEEE KKQLSHPANF GPRKYCLREC ICEVEGQVPC PSLVPLPKEM R GKYKAALK ADAQD

UniProtKB: Small ribosomal subunit protein mS25

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Macromolecule #25: 28S ribosomal protein S26, mitochondrial

MacromoleculeName: 28S ribosomal protein S26, mitochondrial / type: protein_or_peptide / ID: 25 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 24.25982 KDa
SequenceString: MLRALSRLGA GTPCRPRAPL VLPARGRKTR HDPLAKSKIE RVNMPPAVDP AEFFVLMERY QHYRQTVRAL RMEFVSEVQR KVHEARAGV LAERKALKDA AEHRELMAWN QAENRRLHEL RIARLRQEER EQEQRQALEQ ARKAEEVQAW AQRKEREVLQ L QEEVKNFI ...String:
MLRALSRLGA GTPCRPRAPL VLPARGRKTR HDPLAKSKIE RVNMPPAVDP AEFFVLMERY QHYRQTVRAL RMEFVSEVQR KVHEARAGV LAERKALKDA AEHRELMAWN QAENRRLHEL RIARLRQEER EQEQRQALEQ ARKAEEVQAW AQRKEREVLQ L QEEVKNFI TRENLEARVE AALDSRKNYN WAITREGLVV RPQRRDS

UniProtKB: Small ribosomal subunit protein mS26

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Macromolecule #26: 28S ribosomal protein S27, mitochondrial

MacromoleculeName: 28S ribosomal protein S27, mitochondrial / type: protein_or_peptide / ID: 26 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 47.669262 KDa
SequenceString: MAASIVRRGM LLARQVVLPQ LSPAGKRYLL SSAYVDSHKW EAREKEHYCL ADLASLMDKT FERKLPVSSL TISRLIDNIS SREEIDHAE YYLYKFRHSP NCWYLRNWTI HTWIRQCLKY DAQDKALYTL VNKVQYGIFP DNFTFNLLMD SFIKKENYKD A LSVVFEVM ...String:
MAASIVRRGM LLARQVVLPQ LSPAGKRYLL SSAYVDSHKW EAREKEHYCL ADLASLMDKT FERKLPVSSL TISRLIDNIS SREEIDHAE YYLYKFRHSP NCWYLRNWTI HTWIRQCLKY DAQDKALYTL VNKVQYGIFP DNFTFNLLMD SFIKKENYKD A LSVVFEVM MQEAFEVPST QLLSLYVLFH CLAKKTDFSW EEERNFGASL LLPGLKQKNS VGFSSQLYGY ALLGKVELQQ GL RAVYHNM PLIWKPGYLD RALQVMEKVA ASPEDIKLCR EALDVLGAVL KALTSADGAS EEQSQNDEDN QGSEKLVEQL DIE ETEQSK LPQYLERFKA LHSKLQALGK IESEGLLSLT TQLVKEKLST CEAEDIATYE QNLQQWHLDL VQLIQREQQQ REQA KQEYQ AQKAAKASA

UniProtKB: Small ribosomal subunit protein mS27

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Macromolecule #27: 28S ribosomal protein S28, mitochondrial

MacromoleculeName: 28S ribosomal protein S28, mitochondrial / type: protein_or_peptide / ID: 27 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 20.878846 KDa
SequenceString:
MAALCRTRAV AAESHFLRVF LFFRPFRGVG TESGSESGSS NAKEPKTRAG GFASALERHS ELLQKVEPLQ KGSPKNVESF ASMLRHSPL TQMGPAKDKL VIGRIFHIVE NDLYIDFGGK FHCVCRRPEV DGEKYQKGTR VRLRLLDLEL TSRFLGATTD T TVLEANAV LLGIQESKDS RSKEEHHEK

UniProtKB: Small ribosomal subunit protein bS1m

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Macromolecule #28: 28S ribosomal protein S29, mitochondrial

MacromoleculeName: 28S ribosomal protein S29, mitochondrial / type: protein_or_peptide / ID: 28 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 45.634406 KDa
SequenceString: MMLKGITRLI SRIHKLDPGR FLHMGTQARQ SIAAHLDNQV PVESPRAISR TNENDPAKHG DQHEGQHYNI SPQDLETVFP HGLPPRFVM QVKTFSEACL MVRKPALELL HYLKNTSFAY PAIRYLLYGE KGTGKTLSLC HVIHFCAKQD WLILHIPDAH L WVKNCRDL ...String:
MMLKGITRLI SRIHKLDPGR FLHMGTQARQ SIAAHLDNQV PVESPRAISR TNENDPAKHG DQHEGQHYNI SPQDLETVFP HGLPPRFVM QVKTFSEACL MVRKPALELL HYLKNTSFAY PAIRYLLYGE KGTGKTLSLC HVIHFCAKQD WLILHIPDAH L WVKNCRDL LQSSYNKQRF DQPLEASTWL KNFKTTNERF LNQIKVQEKY VWNKRESTEK GSPLGEVVEQ GITRVRNATD AV GIVLKEL KRQSSLGMFH LLVAVDGINA LWGRTTLKRE DKSPIAPEEL ALVHNLRKMM KNDWHGGAIV SALSQTGSLF KPR KAYLPQ ELLGKEGFDA LDPFIPILVS NYNPKEFESC IQYYLENNWL QHEKAPTEEG KKELLFLSNA NPSLLERHCA YL

UniProtKB: Small ribosomal subunit protein mS29

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Macromolecule #29: 28S ribosomal protein S31, mitochondrial

MacromoleculeName: 28S ribosomal protein S31, mitochondrial / type: protein_or_peptide / ID: 29 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 45.391473 KDa
SequenceString: MFPRVSTFLP LRPLSRHPLS SGSPETSAAA IMLLTVRHGT VRYRSSALLA RTKNNIQRYF GTNSVICSKK DKQSVRTEET SKETSESQD SEKENTKKDL LGIIKGMKVE LSTVNVRTTK PPKRRPLKSL EATLGRLRRA TEYAPKKRIE PLSPELVAAA S AVADSLPF ...String:
MFPRVSTFLP LRPLSRHPLS SGSPETSAAA IMLLTVRHGT VRYRSSALLA RTKNNIQRYF GTNSVICSKK DKQSVRTEET SKETSESQD SEKENTKKDL LGIIKGMKVE LSTVNVRTTK PPKRRPLKSL EATLGRLRRA TEYAPKKRIE PLSPELVAAA S AVADSLPF DKQTTKSELL SQLQQHEEES RAQRDAKRPK ISFSNIISDM KVARSATARV RSRPELRIQF DEGYDNYPGQ EK TDDLKKR KNIFTGKRLN IFDMMAVTKE APETDTSPSL WDVEFAKQLA TVNEQPLQNG FEELIQWTKE GKLWEFPINN EAG FDDDGS EFHEHIFLEK HLESFPKQGP IRHFMELVTC GLSKNPYLSV KQKVEHIEWF RNYFNEKKDI LKESNIQFN

UniProtKB: Small ribosomal subunit protein mS31

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Macromolecule #30: 28S ribosomal protein S33, mitochondrial

MacromoleculeName: 28S ribosomal protein S33, mitochondrial / type: protein_or_peptide / ID: 30 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 12.657776 KDa
SequenceString:
MSSLSEYAFR MSRLSARLFG EVTRPTNSKS MKVVKLFSEL PLAKKKETYD WYPNHHTYAE LMQTLRFLGL YRDEHQDFMD EQKRLKKLR GKEKPKKGEG KRAAKRK

UniProtKB: Small ribosomal subunit protein mS33

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Macromolecule #31: Putative ribosome-binding factor A, mitochondrial

MacromoleculeName: Putative ribosome-binding factor A, mitochondrial / type: protein_or_peptide / ID: 31 / Number of copies: 1 / Enantiomer: LEVO
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 38.417434 KDa
SequenceString: MWAAAGGLWR SRAGLRALFR SRDAALFPGC ERGLHCSAVS CKNWLKKFAS KTKKKVWYES PSLGSHSTYK PSKLEFLMRS TSKKTRKED HARLRALNGL LYKALTDLLC TPEVSQELYD LNVELSKVSL TPDFSACRAY WKTTLSAEQN AHMEAVLQRS A AHMRHLLM ...String:
MWAAAGGLWR SRAGLRALFR SRDAALFPGC ERGLHCSAVS CKNWLKKFAS KTKKKVWYES PSLGSHSTYK PSKLEFLMRS TSKKTRKED HARLRALNGL LYKALTDLLC TPEVSQELYD LNVELSKVSL TPDFSACRAY WKTTLSAEQN AHMEAVLQRS A AHMRHLLM SQQTLRNVPP IVFVQDKGNA ALAELDQLLA VADFGPRDER DNFVQNDFRD PDAPQPCGTT EPTTSSSLCG ID HEALNKQ IMEYKRRKDK GLGGLVWQGQ VAELTTQMKK GRKRAKPRLE QDSSLKSYLS GEEVEDDLDL VGAPEYECYA PDT EELEAE RGGGRTEDGH SCGASRE

UniProtKB: Putative ribosome-binding factor A, mitochondrial

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Macromolecule #32: 12S rRNA N4-methylcytidine (m4C) methyltransferase

MacromoleculeName: 12S rRNA N4-methylcytidine (m4C) methyltransferase / type: protein_or_peptide / ID: 32 / Number of copies: 1 / Enantiomer: LEVO
EC number: Transferases; Transferring one-carbon groups; Methyltransferases
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 46.18402 KDa
SequenceString: MLRYPYFCRM YKECLSCWLE SGIPNLGVWP NRIHTTAEKY REYEAREQTD QTQAQELHRS QDRDFETMAK LHIPVMVDEV VHCLSPQKG QIFLDMTFGS GGHTKAILQK ESDIVLYALD RDPTAYALAE HLSELYPKQI RAMLGQFSQA EALLMKAGVQ P GTFDGVLM ...String:
MLRYPYFCRM YKECLSCWLE SGIPNLGVWP NRIHTTAEKY REYEAREQTD QTQAQELHRS QDRDFETMAK LHIPVMVDEV VHCLSPQKG QIFLDMTFGS GGHTKAILQK ESDIVLYALD RDPTAYALAE HLSELYPKQI RAMLGQFSQA EALLMKAGVQ P GTFDGVLM DLGCSSMQLD TPERGFSLRK DGPLDMRMDG GRYPDMPTAA DVVNALDQQA LASILRTYGE EKHAKKIASA IV QARSIYP ITRTQQLASI VAGAFPPSAI YTRKDLLQRS THIATKTFQA LRIFVNNELN ELYTGLKTAQ KFLRPGGRLV ALS FHSLED RIVKRFLLGI SMTERFNLSV RQQVMKTSQL GSDHENTEEV SMRRAPLMWE LIHKKVLSPQ DQDVQDNPRG RSAK LRAAI KL

UniProtKB: 12S rRNA N(4)-cytidine methyltransferase METTL15

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Macromolecule #5: 12S mitochondrial rRNA

MacromoleculeName: 12S mitochondrial rRNA / type: rna / ID: 5 / Number of copies: 1
Source (natural)Organism: Homo sapiens (human)
Molecular weightTheoretical: 306.449312 KDa
SequenceString: AAUAGGUUUG GUCCUAGCCU UUCUAUUAGC UCUUAGUAAG AUUACACAUG CAAGCAUCCC CGUUCCAGUG AGUUCACCCU CUAAAUCAC CACGAUCAAA AGGGACAAGC AUCAAGCACG CAGCAAUGCA GCUCAAAACG CUUAGCCUAG CCACACCCCC A CGGGAAAC ...String:
AAUAGGUUUG GUCCUAGCCU UUCUAUUAGC UCUUAGUAAG AUUACACAUG CAAGCAUCCC CGUUCCAGUG AGUUCACCCU CUAAAUCAC CACGAUCAAA AGGGACAAGC AUCAAGCACG CAGCAAUGCA GCUCAAAACG CUUAGCCUAG CCACACCCCC A CGGGAAAC AGCAGUGAUU AACCUUUAGC AAUAAACGAA AGUUUAACUA AGCUAUACUA ACCCCAGGGU UGGUCAAUUU CG UGCCAGC CACCGCGGUC ACACGAUUAA CCCAAGUCAA UAGAAGCCGG CGUAAAGAGU GUUUUAGAUC ACCCCCUCCC CAA UAAAGC UAAAACUCAC CUGAGUUGUA AAAAACUCCA GUUGACACAA AAUAGACUAC GAAAGUGGCU UUAACAUAUC UGAA CACAC AAUAGCUAAG ACCCAAACUG GGAUUAGAUA CCCCACUAUG CUUAGCCCUA AACCUCAACA GUUAAAUCAA CAAAA CUGC UCGCCAGAAC ACUACGAGCC ACAGCUUAAA ACUCAAAGGA CCUGGCGGUG CUUCAUAUCC CUCUAGAGGA GCCUGU UCU GUAAUCGAUA AACCCCGAUC AACCUCACCA CCUCUUGCUC AGCCUAUAUA CCGCCAUCUU CAGCAAACCC UGAUGAA GG CUACAAAGUA AGCGCAAGUA CCCACGUAAA GACGUUAGGU CAAGGUGUAG CCCAUGAGGU GGCAAGAAAU GGGCUACA U UUUCUACCCC AGAAAACUAC GAUAGCCCUU AUGAAACUUA AGGGUCGAAG GUGGAUUUAG CAGUAAACUG AGAGUAGAG UGCUUAGUUG AACAGGGCCC UGAAGCGCGU ACACACCGCC CGUCACCCUC CUCAAGUAUA CUUCAAAGGA CAUUUAACUA AAACCCCUA CGCAUUUAUA UAGAGGAGAC AAGUCGUAAC AUGGUAAGUG UACUGGAAAG UGCACUUGGA CGAACC

GENBANK: GENBANK: MN849517.1

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Macromolecule #33: MAGNESIUM ION

MacromoleculeName: MAGNESIUM ION / type: ligand / ID: 33 / Number of copies: 10 / Formula: MG
Molecular weightTheoretical: 24.305 Da

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Macromolecule #34: POTASSIUM ION

MacromoleculeName: POTASSIUM ION / type: ligand / ID: 34 / Number of copies: 1 / Formula: K
Molecular weightTheoretical: 39.098 Da

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Macromolecule #35: NICOTINAMIDE-ADENINE-DINUCLEOTIDE

MacromoleculeName: NICOTINAMIDE-ADENINE-DINUCLEOTIDE / type: ligand / ID: 35 / Number of copies: 1 / Formula: NAD
Molecular weightTheoretical: 663.425 Da
Chemical component information

ChemComp-NAD:
NICOTINAMIDE-ADENINE-DINUCLEOTIDE / NAD*YM

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Macromolecule #36: ZINC ION

MacromoleculeName: ZINC ION / type: ligand / ID: 36 / Number of copies: 1 / Formula: ZN
Molecular weightTheoretical: 65.409 Da

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Macromolecule #37: FE2/S2 (INORGANIC) CLUSTER

MacromoleculeName: FE2/S2 (INORGANIC) CLUSTER / type: ligand / ID: 37 / Number of copies: 2 / Formula: FES
Molecular weightTheoretical: 175.82 Da
Chemical component information

ChemComp-FES:
FE2/S2 (INORGANIC) CLUSTER

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Macromolecule #38: GUANOSINE-5'-DIPHOSPHATE

MacromoleculeName: GUANOSINE-5'-DIPHOSPHATE / type: ligand / ID: 38 / Number of copies: 1 / Formula: GDP
Molecular weightTheoretical: 443.201 Da
Chemical component information

ChemComp-GDP:
GUANOSINE-5'-DIPHOSPHATE / GDP, energy-carrying molecule*YM

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Macromolecule #39: ADENOSINE-5'-TRIPHOSPHATE

MacromoleculeName: ADENOSINE-5'-TRIPHOSPHATE / type: ligand / ID: 39 / Number of copies: 1 / Formula: ATP
Molecular weightTheoretical: 507.181 Da
Chemical component information

ChemComp-ATP:
ADENOSINE-5'-TRIPHOSPHATE / ATP, energy-carrying molecule*YM

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Macromolecule #40: S-ADENOSYL-L-HOMOCYSTEINE

MacromoleculeName: S-ADENOSYL-L-HOMOCYSTEINE / type: ligand / ID: 40 / Number of copies: 1 / Formula: SAH
Molecular weightTheoretical: 384.411 Da
Chemical component information

ChemComp-SAH:
S-ADENOSYL-L-HOMOCYSTEINE

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Experimental details

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Structure determination

Methodcryo EM
Processingsingle particle reconstruction
Aggregation stateparticle

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Sample preparation

BufferpH: 7.4
Component:
ConcentrationNameFormula
20.0 mMHepes-HCl
100.0 mMPotassium chlorideKCl
20.0 mMMagnesium chlorideMgCl2
0.02 %DDM
1.0 mMPMSF
1.0 Xprotease-inhibitor mix
0.5 mMGMP-PNP

Details: 20mM HEPES-HCl, 100mM KCl, 20mM MgCl2, 0.02% DDM, 1mM PMSF, 1x protease-inhibitor mix, 0.5mM GMP-PNP
GridModel: Quantifoil R3.5/1 / Material: COPPER / Support film - #0 - Film type ID: 1 / Support film - #0 - Material: CARBON / Support film - #0 - topology: HOLEY / Support film - #1 - Film type ID: 2 / Support film - #1 - Material: CARBON / Support film - #1 - topology: CONTINUOUS / Pretreatment - Type: GLOW DISCHARGE / Details: The grid was precoated with a 2-3 nm carbon layer.
VitrificationCryogen name: ETHANE / Chamber humidity: 95 % / Chamber temperature: 277.15 K / Instrument: FEI VITROBOT MARK IV
DetailsThe sample was crosslinked with 0.15% glutaraldehyde for 10 min on ice. The reaction was stopped by adding 50 mM lysine pH 7.5 and 50 mM aspartate pH 7.5, and subsequently desalted against the final buffer prior to vitrification.

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Electron microscopy

MicroscopeTFS KRIOS
Image recordingFilm or detector model: GATAN K3 BIOQUANTUM (6k x 4k) / Average exposure time: 3.0 sec. / Average electron dose: 40.0 e/Å2
Electron beamAcceleration voltage: 300 kV / Electron source: FIELD EMISSION GUN
Electron opticsIllumination mode: OTHER / Imaging mode: BRIGHT FIELD / Cs: 2.7 mm / Nominal defocus max: 1.6 µm / Nominal defocus min: 0.6 µm / Nominal magnification: 81000
Sample stageSpecimen holder model: FEI TITAN KRIOS AUTOGRID HOLDER / Cooling holder cryogen: NITROGEN
Experimental equipment
Model: Titan Krios / Image courtesy: FEI Company

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Image processing

Particle selectionNumber selected: 5730547
Startup modelType of model: NONE / Details: ab initio reconstruction after 2D classification
Final reconstructionNumber classes used: 1 / Algorithm: FOURIER SPACE / Resolution.type: BY AUTHOR / Resolution: 3.0 Å / Resolution method: FSC 0.143 CUT-OFF / Software - Name: cryoSPARC (ver. 4.4.1.) / Number images used: 79037
Initial angle assignmentType: MAXIMUM LIKELIHOOD / Software - Name: RELION (ver. 3.0)
Final angle assignmentType: MAXIMUM LIKELIHOOD / Software - Name: RELION (ver. 3.0)
Final 3D classificationNumber classes: 8 / Avg.num./class: 77919 / Software - Name: RELION (ver. 3.0)

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Atomic model buiding 1

Initial model
PDB IDChain

7pnx

chain_id: A, source_name: PDB, initial_model_type: experimental model

7pnx

chain_id: B, source_name: PDB, initial_model_type: experimental model

7pnx

chain_id: C, source_name: PDB, initial_model_type: experimental model

7pnx

chain_id: D, source_name: PDB, initial_model_type: experimental model

7pnx

chain_id: E, source_name: PDB, initial_model_type: experimental model

7pnx

chain_id: F, source_name: PDB, initial_model_type: experimental model

7pnx

chain_id: G, source_name: PDB, initial_model_type: experimental model

7pnx

chain_id: H, source_name: PDB, initial_model_type: experimental model

7pnx

chain_id: I, source_name: PDB, initial_model_type: experimental model

7pnx

chain_id: J, source_name: PDB, initial_model_type: experimental model

7pnx

chain_id: K, source_name: PDB, initial_model_type: experimental model

7pnx

chain_id: L, source_name: PDB, initial_model_type: experimental model

7pnx

chain_id: M, source_name: PDB, initial_model_type: experimental model

7pnx

chain_id: N, source_name: PDB, initial_model_type: experimental model

7pnx

chain_id: O, source_name: PDB, initial_model_type: experimental model

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chain_id: P, source_name: PDB, initial_model_type: experimental model

7pnx

chain_id: Q, source_name: PDB, initial_model_type: experimental model

7pnx

chain_id: R, source_name: PDB, initial_model_type: experimental model

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chain_id: S, source_name: PDB, initial_model_type: experimental model

7pnx

chain_id: T, source_name: PDB, initial_model_type: experimental model

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chain_id: U, source_name: PDB, initial_model_type: experimental model

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chain_id: V, source_name: PDB, initial_model_type: experimental model

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chain_id: W, source_name: PDB, initial_model_type: experimental model

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chain_id: X, source_name: PDB, initial_model_type: experimental model

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chain_id: Y, source_name: PDB, initial_model_type: experimental model

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chain_id: Z, source_name: PDB, initial_model_type: experimental model

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chain_id: 0, source_name: PDB, initial_model_type: experimental model

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chain_id: 1, source_name: PDB, initial_model_type: experimental model

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chain_id: 3, source_name: PDB, initial_model_type: experimental model

7pnx

chain_id: 4, source_name: PDB, initial_model_type: experimental model

7pnx

chain_id: a, source_name: PDB, initial_model_type: experimental model

7pnx

chain_id: b, source_name: PDB, initial_model_type: experimental model
RefinementSpace: REAL / Protocol: RIGID BODY FIT
Output model

PDB-9g5d:
Assembly intermediate of human mitochondrial ribosome small subunit (State C)

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