EMDB/PDB/SASBDBから引用されている文献のデータベース

キーワード
構造解析手法	All X線回折 NMR 電子顕微鏡小角散乱中性子線回折線維回折粉末回折赤外分光 EPR FRET 理論モデルハイブリッド
著者・登録者
ジャーナル
IF	>30 >20 >10 >5 all

タイトル	In-cell architecture of the mitochondrial respiratory chain.
ジャーナル・号・ページ	Science, Vol. 387, Issue 6740, Page 1296-1301, Year 2025
掲載日	2025年3月21日
著者	Florent Waltz / Ricardo D Righetto / Lorenz Lamm / Thalia Salinas-Giegé / Ron Kelley / Xianjun Zhang / Martin Obr / Sagar Khavnekar / Abhay Kotecha / Benjamin D Engel /
PubMed 要旨	Mitochondria regenerate adenosine triphosphate (ATP) through oxidative phosphorylation. This process is carried out by five membrane-bound complexes collectively known as the respiratory chain, ...Mitochondria regenerate adenosine triphosphate (ATP) through oxidative phosphorylation. This process is carried out by five membrane-bound complexes collectively known as the respiratory chain, working in concert to transfer electrons and pump protons. The precise organization of these complexes in native cells is debated. We used in situ cryo-electron tomography to visualize the native structures and organization of several major mitochondrial complexes in cells. ATP synthases and respiratory complexes segregate into curved and flat crista membrane domains, respectively. Respiratory complexes I, III, and IV assemble into a respirasome supercomplex, from which we determined a native 5-angstrom (Å) resolution structure showing binding of electron carrier cytochrome . Combined with single-particle cryo-electron microscopy at 2.4-Å resolution, we model how the respiratory complexes organize inside native mitochondria.
リンク	Science / PubMed:40112058
手法	EM (単粒子) / EM (サブトモグラム平均)
解像度	2.21 - 28.5 Å
構造データ	50202 9f5x EMDB-50202, PDB-9f5x: Structure of the Chlamydomonas reinhardtii respiratory supercomplex I1 III2 IV2 手法: EM (単粒子) / 解像度: 2.82 Å 50203 9f5y EMDB-50203, PDB-9f5y: Structure of the Chlamydomonas reinhardtii respiratory complex I from respiratory supercomplex 手法: EM (単粒子) / 解像度: 2.51 Å 50204 9f5z EMDB-50204, PDB-9f5z: Structure of the Chlamydomonas reinhardtii respiratory complex III from respiratory supercomplex 手法: EM (単粒子) / 解像度: 2.39 Å 50205 9f60 EMDB-50205, PDB-9f60: Structure of the Chlamydomonas reinhardtii respiratory complex IV from respiratory supercomplex 手法: EM (単粒子) / 解像度: 2.39 Å 50206 9f61 EMDB-50206, PDB-9f61: Structure of the Chlamydomonas reinhardtii respiratory complex IV from respiratory supercomplex 手法: EM (単粒子) / 解像度: 2.55 Å EMDB-50207: Structure of the Chlamydomonas reinhardtii respiratory supercomplex, focused on complex I matrix part 手法: EM (単粒子) / 解像度: 2.34 Å EMDB-50208: Structure of the Chlamydomonas reinhardtii respiratory supercomplex, focused on complex I proximal membrane part 手法: EM (単粒子) / 解像度: 2.3 Å EMDB-50209: Structure of the Chlamydomonas reinhardtii respiratory supercomplex, focused on complex I distal membrane part 手法: EM (単粒子) / 解像度: 2.21 Å 50210 9f62 EMDB-50210: Subtomogram average of the Chlamydomonas reinhardtii mitochondrial respirasome - C2 symmetry PDB-9f62: Subtomogram average of the Chlamydomonas reinhardtii mitochondrial respirasome I2 III4 IV6 手法: EM (サブトモグラム平均) / 解像度: 5.44 Å EMDB-50211: Subtomogram average of the Chlamydomonas reinhardtii mitochondrial respirasome - C2 expanded 手法: EM (サブトモグラム平均) / 解像度: 6.18 Å EMDB-50212: Structure of the putative Chlamydomonas mitochondrial prohibitin 手法: EM (サブトモグラム平均) / 解像度: 28.5 Å EMDB-50213: Subtotomogram average of Chlamydomonas mitochondrial ribosome 手法: EM (サブトモグラム平均) / 解像度: 21.8 Å EMDB-50214: Subtomogram average of the Chlamydomonas reinhardtii mitochondrial respirasome - C2 expanded, complex I focus 手法: EM (サブトモグラム平均) / 解像度: 5.6 Å EMDB-50215: Subtomogram average of the Chlamydomonas reinhardtii mitochondrial respirasome - C2 expanded, complex III focus 手法: EM (サブトモグラム平均) / 解像度: 6.2 Å EMDB-50216: Subtomogram average of the Chlamydomonas reinhardtii mitochondrial respirasome - C1 symmetry 手法: EM (サブトモグラム平均) / 解像度: 7.05 Å EMDB-50248: Structure of the Chlamydomonas reinhardtii respiratory supercomplex, focused on complex I 手法: EM (単粒子) / 解像度: 2.51 Å EMDB-50249: Structure of the Chlamydomonas reinhardtii respiratory supercomplex I1 III2 IV2 手法: EM (単粒子) / 解像度: 2.82 Å
化合物	ChemComp-UQ5: 2,3-DIMETHOXY-5-METHYL-6-(3,11,15,19-TETRAMETHYL-EICOSA-2,6,10,14,18-PENTAENYL)-[1,4]BENZOQUINONE / ユビキノン5 ChemComp-HEM: PROTOPORPHYRIN IX CONTAINING FE ChemComp-CDL: CARDIOLIPIN / リン脂質YM ChemComp-3PH: 1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE / ジステアロイルホスファチジン酸 ChemComp-PTY: PHOSPHATIDYLETHANOLAMINE / リン脂質YM ChemComp-HEC: HEME C ChemComp-PC7: (7S)-4-HYDROXY-N,N,N-TRIMETHYL-9-OXO-7-[(PALMITOYLOXY)METHYL]-3,5,8-TRIOXA-4-PHOSPHAHEXACOSAN-1-AMINIUM 4-OXIDE / リン脂質YM ChemComp-ZN: Unknown entry ChemComp-PGT: (1S)-2-{[{[(2R)-2,3-DIHYDROXYPROPYL]OXY}(HYDROXY)PHOSPHORYL]OXY}-1-[(PALMITOYLOXY)METHYL]ETHYL STEARATE / リン脂質YM ChemComp-HEA: HEME-A ChemComp-CU: COPPER (II) ION ChemComp-MG: Unknown entry / マグネシウムジカチオン ChemComp-CUA: DINUCLEAR COPPER ION ChemComp-FES: FE2/S2 (INORGANIC) CLUSTER ChemComp-FMN: FLAVIN MONONUCLEOTIDE / FMN ChemComp-SF4: IRON/SULFUR CLUSTER ChemComp-8Q1: S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate ChemComp-NDP: NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE / NADPH ChemComp-COO: CROTONYL COENZYME A ChemComp-HOH: WATER
由来	chlamydomonas reinhardtii (クラミドモナス) Brassica oleracea var. botrytis (カリフラワー)
キーワード	MEMBRANE PROTEIN / Mitochondria / respiratory complex / respiration / alga / mitoribosome / assembly factor / plant / RIBOSOME / respirasome