Database of articles cited by EMDB/PDB/SASBDB data

Keywords
Structure methods	All X-ray diffraction NMR electron microscopy small angle scattering neutron diffraction fiber diffraction powder diffraction infrared spectroscopy EPR fluorescence transfer theoretical model Hybrid
Author
Journal
IF	>30 >20 >10 >5 all

Title	In-cell architecture of the mitochondrial respiratory chain.
Journal, issue, pages	Science, Vol. 387, Issue 6740, Page 1296-1301, Year 2025
Publish date	Mar 21, 2025
Authors	Florent Waltz / Ricardo D Righetto / Lorenz Lamm / Thalia Salinas-Giegé / Ron Kelley / Xianjun Zhang / Martin Obr / Sagar Khavnekar / Abhay Kotecha / Benjamin D Engel /
PubMed Abstract	Mitochondria regenerate adenosine triphosphate (ATP) through oxidative phosphorylation. This process is carried out by five membrane-bound complexes collectively known as the respiratory chain, ...Mitochondria regenerate adenosine triphosphate (ATP) through oxidative phosphorylation. This process is carried out by five membrane-bound complexes collectively known as the respiratory chain, working in concert to transfer electrons and pump protons. The precise organization of these complexes in native cells is debated. We used in situ cryo-electron tomography to visualize the native structures and organization of several major mitochondrial complexes in cells. ATP synthases and respiratory complexes segregate into curved and flat crista membrane domains, respectively. Respiratory complexes I, III, and IV assemble into a respirasome supercomplex, from which we determined a native 5-angstrom (Å) resolution structure showing binding of electron carrier cytochrome . Combined with single-particle cryo-electron microscopy at 2.4-Å resolution, we model how the respiratory complexes organize inside native mitochondria.
External links	Science / PubMed:40112058
Methods	EM (single particle) / EM (subtomogram averaging)
Resolution	2.21 - 28.5 Å
Structure data	50202 9f5x EMDB-50202, PDB-9f5x: Structure of the Chlamydomonas reinhardtii respiratory supercomplex I1 III2 IV2 Method: EM (single particle) / Resolution: 2.82 Å 50203 9f5y EMDB-50203, PDB-9f5y: Structure of the Chlamydomonas reinhardtii respiratory complex I from respiratory supercomplex Method: EM (single particle) / Resolution: 2.51 Å 50204 9f5z EMDB-50204, PDB-9f5z: Structure of the Chlamydomonas reinhardtii respiratory complex III from respiratory supercomplex Method: EM (single particle) / Resolution: 2.39 Å 50205 9f60 EMDB-50205, PDB-9f60: Structure of the Chlamydomonas reinhardtii respiratory complex IV from respiratory supercomplex Method: EM (single particle) / Resolution: 2.39 Å 50206 9f61 EMDB-50206, PDB-9f61: Structure of the Chlamydomonas reinhardtii respiratory complex IV from respiratory supercomplex Method: EM (single particle) / Resolution: 2.55 Å EMDB-50207: Structure of the Chlamydomonas reinhardtii respiratory supercomplex, focused on complex I matrix part Method: EM (single particle) / Resolution: 2.34 Å EMDB-50208: Structure of the Chlamydomonas reinhardtii respiratory supercomplex, focused on complex I proximal membrane part Method: EM (single particle) / Resolution: 2.3 Å EMDB-50209: Structure of the Chlamydomonas reinhardtii respiratory supercomplex, focused on complex I distal membrane part Method: EM (single particle) / Resolution: 2.21 Å 50210 9f62 EMDB-50210: Subtomogram average of the Chlamydomonas reinhardtii mitochondrial respirasome - C2 symmetry PDB-9f62: Subtomogram average of the Chlamydomonas reinhardtii mitochondrial respirasome I2 III4 IV6 Method: EM (subtomogram averaging) / Resolution: 5.44 Å EMDB-50211: Subtomogram average of the Chlamydomonas reinhardtii mitochondrial respirasome - C2 expanded Method: EM (subtomogram averaging) / Resolution: 6.18 Å EMDB-50212: Structure of the putative Chlamydomonas mitochondrial prohibitin Method: EM (subtomogram averaging) / Resolution: 28.5 Å EMDB-50213: Subtotomogram average of Chlamydomonas mitochondrial ribosome Method: EM (subtomogram averaging) / Resolution: 21.8 Å EMDB-50214: Subtomogram average of the Chlamydomonas reinhardtii mitochondrial respirasome - C2 expanded, complex I focus Method: EM (subtomogram averaging) / Resolution: 5.6 Å EMDB-50215: Subtomogram average of the Chlamydomonas reinhardtii mitochondrial respirasome - C2 expanded, complex III focus Method: EM (subtomogram averaging) / Resolution: 6.2 Å EMDB-50216: Subtomogram average of the Chlamydomonas reinhardtii mitochondrial respirasome - C1 symmetry Method: EM (subtomogram averaging) / Resolution: 7.05 Å EMDB-50248: Structure of the Chlamydomonas reinhardtii respiratory supercomplex, focused on complex I Method: EM (single particle) / Resolution: 2.51 Å EMDB-50249: Structure of the Chlamydomonas reinhardtii respiratory supercomplex I1 III2 IV2 Method: EM (single particle) / Resolution: 2.82 Å
Chemicals	ChemComp-UQ5: 2,3-DIMETHOXY-5-METHYL-6-(3,11,15,19-TETRAMETHYL-EICOSA-2,6,10,14,18-PENTAENYL)-[1,4]BENZOQUINONE ChemComp-HEM: PROTOPORPHYRIN IX CONTAINING FE ChemComp-CDL: CARDIOLIPIN / phospholipidYM ChemComp-3PH: 1,2-DIACYL-GLYCEROL-3-SN-PHOSPHATE ChemComp-PTY: PHOSPHATIDYLETHANOLAMINE / phospholipidYM ChemComp-HEC: HEME C ChemComp-PC7: (7S)-4-HYDROXY-N,N,N-TRIMETHYL-9-OXO-7-[(PALMITOYLOXY)METHYL]-3,5,8-TRIOXA-4-PHOSPHAHEXACOSAN-1-AMINIUM 4-OXIDE / phospholipidYM ChemComp-ZN: Unknown entry ChemComp-PGT: (1S)-2-{[{[(2R)-2,3-DIHYDROXYPROPYL]OXY}(HYDROXY)PHOSPHORYL]OXY}-1-[(PALMITOYLOXY)METHYL]ETHYL STEARATE / phospholipidYM ChemComp-HEA: HEME-A ChemComp-CU: COPPER (II) ION ChemComp-MG: Unknown entry ChemComp-CUA: DINUCLEAR COPPER ION ChemComp-FES: FE2/S2 (INORGANIC) CLUSTER ChemComp-FMN: FLAVIN MONONUCLEOTIDE ChemComp-SF4: IRON/SULFUR CLUSTER ChemComp-8Q1: S-[2-({N-[(2R)-2-hydroxy-3,3-dimethyl-4-(phosphonooxy)butanoyl]-beta-alanyl}amino)ethyl] dodecanethioate ChemComp-NDP: NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE ChemComp-COO: CROTONYL COENZYME A ChemComp-HOH: WATER
Source	chlamydomonas reinhardtii (plant) Brassica oleracea var. botrytis (cauliflower)
Keywords	MEMBRANE PROTEIN / Mitochondria / respiratory complex / respiration / alga / mitoribosome / assembly factor / plant / RIBOSOME / respirasome