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TitleStructure and function of a near fully-activated intermediate GPCR-Gαβγ complex.
Journal, issue, pagesNat Commun, Vol. 16, Issue 1, Page 1100, Year 2025
Publish dateJan 28, 2025
AuthorsMaxine Bi / Xudong Wang / Jinan Wang / Jun Xu / Wenkai Sun / Victor Ayo Adediwura / Yinglong Miao / Yifan Cheng / Libin Ye /
PubMed AbstractUnraveling the signaling roles of intermediate complexes is pivotal for G protein-coupled receptor (GPCR) drug development. Despite hundreds of GPCR-Gαβγ structures, these snapshots primarily ...Unraveling the signaling roles of intermediate complexes is pivotal for G protein-coupled receptor (GPCR) drug development. Despite hundreds of GPCR-Gαβγ structures, these snapshots primarily capture the fully activated complex. Consequently, the functions of intermediate GPCR-G protein complexes remain elusive. Guided by a conformational landscape visualized via F quantitative NMR and molecular dynamics (MD) simulations, we determined the structure of an intermediate GPCR-mini-Gαβγ complex at 2.6 Å using cryo-EM, by blocking its transition to the fully activated complex. Furthermore, we present direct evidence that the complex at this intermediate state initiates a rate-limited nucleotide exchange before transitioning to the fully activated complex. In this state, BODIPY-GDP/GTP based nucleotide exchange assays further indicated the α-helical domain of the Gα is partially open, allowing it to grasp a nucleotide at a non-canonical binding site, distinct from the canonical nucleotide-binding site. These advances bridge a significant gap in our understanding of the complexity of GPCR signaling.
External linksNat Commun / PubMed:39875358 / PubMed Central
MethodsEM (single particle)
Resolution2.63 - 3.36 Å
Structure data

47951

9ee8

EMDB-47951, PDB-9ee8:
Cryo-EM structure of the adenosine A2A receptor intermediate bound to a miniGs heterotrimer
Method: EM (single particle) / Resolution: 2.63 Å

47952

9ee9

EMDB-47952, PDB-9ee9:
Cryo-EM structure of the adenosine A2A receptor intermediate bound to a miniGs heterotrimer
Method: EM (single particle) / Resolution: 3.16 Å

47953

9eea

EMDB-47953, PDB-9eea:
Cryo-EM structure of the adenosine A2A receptor intermediate bound to a miniGs heterotrimer
Method: EM (single particle) / Resolution: 3.36 Å

Chemicals

ChemComp-ADN:
ADENOSINE

Source
  • homo sapiens (human)
  • lama glama (llama)
KeywordsSIGNALING PROTEIN/IMMUNE SYSTEM / GPCR / Heterotrimer / Receptor / cryo-EM / SIGNALING PROTEIN-IMMUNE SYSTEM complex

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